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Protein

Phosphatidylinositol transfer protein beta isoform

Gene

Pitpnb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of PtdIns and phosphatidylcholine between membranes.

GO - Molecular functioni

  • lipid binding Source: UniProtKB-KW
  • phosphatidylinositol transporter activity Source: RGD
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

ReactomeiR-RNO-1483196. PI and PC transport between ER and Golgi membranes.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol transfer protein beta isoform
Short name:
PI-TP-beta
Short name:
PtdIns transfer protein beta
Short name:
PtdInsTP beta
Gene namesi
Name:Pitpnb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi620143. Pitpnb.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 271270Phosphatidylinositol transfer protein beta isoformPRO_0000191646Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei215 – 2151N6-acetyllysineBy similarity
Modified residuei262 – 2621PhosphoserineBy similarity

Post-translational modificationi

Constitutive phosphorylation of Ser-262 has no effect on phospholipid transfer activity but is required for Golgi targeting.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP53812.
PRIDEiP53812.

PTM databases

iPTMnetiP53812.
PhosphoSiteiP53812.

Expressioni

Tissue specificityi

Expressed abundantly in brain, kidney, liver, and lung, but in a lesser amount in testis.

Gene expression databases

ExpressionAtlasiP53812. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000050368.

Structurei

Secondary structure

1
271
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 1311Combined sources
Helixi15 – 3117Combined sources
Beta strandi38 – 4811Combined sources
Beta strandi55 – 639Combined sources
Turni65 – 673Combined sources
Helixi70 – 756Combined sources
Beta strandi82 – 909Combined sources
Beta strandi93 – 997Combined sources
Turni101 – 1033Combined sources
Helixi104 – 1063Combined sources
Beta strandi107 – 12014Combined sources
Helixi130 – 1334Combined sources
Beta strandi136 – 1416Combined sources
Helixi146 – 1483Combined sources
Turni151 – 1533Combined sources
Helixi156 – 1583Combined sources
Helixi160 – 1623Combined sources
Turni166 – 1683Combined sources
Helixi177 – 1826Combined sources
Beta strandi190 – 20011Combined sources
Turni203 – 2053Combined sources
Helixi206 – 23025Combined sources
Helixi232 – 2354Combined sources
Helixi240 – 25718Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2A1LX-ray2.18A2-271[»]
ProteinModelPortaliP53812.
SMRiP53812. Positions 2-271.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53812.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3668. Eukaryota.
COG5083. LUCA.
HOGENOMiHOG000006717.
HOVERGENiHBG058915.
InParanoidiP53812.

Family and domain databases

Gene3Di3.30.530.20. 1 hit.
InterProiIPR001666. PI_transfer.
IPR023393. START-like_dom.
[Graphical view]
PANTHERiPTHR10658. PTHR10658. 1 hit.
PfamiPF02121. IP_trans. 1 hit.
[Graphical view]
PRINTSiPR00391. PITRANSFER.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P53812-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVLIKEFRVV LPCSVQEYQV GQLYSVAEAS KNETGGGEGI EVLKNEPYEN
60 70 80 90 100
DGEKGQYTHK IYHLKSKVPA FVRMIAPEGS LVFHEKAWNA YPYCRTIVTN
110 120 130 140 150
EYMKDDFFIK IETWHKPDLG TLENVHGLDP NTWKTVEIVH IDIADRSQVE
160 170 180 190 200
PADYKADEDP ALFQSVKTKR GPLGPNWKKE LANTPDCPKM CAYKLVTIKF
210 220 230 240 250
KWWGLQSKVE NFIQKQEKRI FTNLHRQLFC WIDKWIDLTM EDIRRMEDET
260 270
QKELETMRKK GSVRGTSAAD A
Length:271
Mass (Da):31,450
Last modified:January 23, 2007 - v2
Checksum:i936F9C5E068422B6
GO
Isoform 2 (identifier: P53812-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     257-271: MRKKGSVRGTSAADA → LRSQGQVRGTSAACDD

Show »
Length:272
Mass (Da):31,579
Checksum:iF184190642EAFC23
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei257 – 27115MRKKG…SAADA → LRSQGQVRGTSAACDD in isoform 2. CuratedVSP_012763Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21132 mRNA. Translation: BAA04669.1.
BC061538 mRNA. Translation: AAH61538.1.
PIRiJX0316.
RefSeqiNP_446194.1. NM_053742.1. [P53812-1]
XP_006249584.1. XM_006249522.2. [P53812-2]
UniGeneiRn.2399.

Genome annotation databases

EnsembliENSRNOT00000066892; ENSRNOP00000058957; ENSRNOG00000000665. [P53812-1]
GeneIDi114561.
KEGGirno:114561.
UCSCiRGD:620143. rat. [P53812-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21132 mRNA. Translation: BAA04669.1.
BC061538 mRNA. Translation: AAH61538.1.
PIRiJX0316.
RefSeqiNP_446194.1. NM_053742.1. [P53812-1]
XP_006249584.1. XM_006249522.2. [P53812-2]
UniGeneiRn.2399.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2A1LX-ray2.18A2-271[»]
ProteinModelPortaliP53812.
SMRiP53812. Positions 2-271.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000050368.

PTM databases

iPTMnetiP53812.
PhosphoSiteiP53812.

Proteomic databases

PaxDbiP53812.
PRIDEiP53812.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000066892; ENSRNOP00000058957; ENSRNOG00000000665. [P53812-1]
GeneIDi114561.
KEGGirno:114561.
UCSCiRGD:620143. rat. [P53812-1]

Organism-specific databases

CTDi23760.
RGDi620143. Pitpnb.

Phylogenomic databases

eggNOGiKOG3668. Eukaryota.
COG5083. LUCA.
HOGENOMiHOG000006717.
HOVERGENiHBG058915.
InParanoidiP53812.

Enzyme and pathway databases

ReactomeiR-RNO-1483196. PI and PC transport between ER and Golgi membranes.

Miscellaneous databases

EvolutionaryTraceiP53812.
PROiP53812.

Gene expression databases

ExpressionAtlasiP53812. baseline and differential.

Family and domain databases

Gene3Di3.30.530.20. 1 hit.
InterProiIPR001666. PI_transfer.
IPR023393. START-like_dom.
[Graphical view]
PANTHERiPTHR10658. PTHR10658. 1 hit.
PfamiPF02121. IP_trans. 1 hit.
[Graphical view]
PRINTSiPR00391. PITRANSFER.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a cDNA encoding a second phosphatidylinositol transfer protein of rat brain by complementation of the yeast sec14 mutation."
    Tanaka S., Hosaka K.
    J. Biochem. 115:981-984(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Wistar.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.

Entry informationi

Entry nameiPIPNB_RAT
AccessioniPrimary (citable) accession number: P53812
Secondary accession number(s): Q6P7S3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.