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Protein

Phosphatidylinositol transfer protein beta isoform

Gene

Pitpnb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of PtdIns and phosphatidylcholine between membranes.

GO - Molecular functioni

GO - Biological processi

  • in utero embryonic development Source: MGI
  • transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-1483196. PI and PC transport between ER and Golgi membranes.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol transfer protein beta isoform
Short name:
PI-TP-beta
Short name:
PtdIns transfer protein beta
Short name:
PtdInsTP beta
Gene namesi
Name:Pitpnb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1927542. Pitpnb.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi165 – 1651S → A: No effect on phosphorylation by PKC; abolishes phospholipid transfer activity. 1 Publication
Mutagenesisi262 – 2621S → A: Abolishes phosphorylation by PKC and impairs Golgi targeting; no effect on phospholipid transfer activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 271270Phosphatidylinositol transfer protein beta isoformPRO_0000191644Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei215 – 2151N6-acetyllysineBy similarity
Modified residuei262 – 2621Phosphoserine; by PKC1 Publication

Post-translational modificationi

Constitutive phosphorylation of Ser-262 has no effect on phospholipid transfer activity but is required for Golgi targeting.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP53811.
MaxQBiP53811.
PaxDbiP53811.
PeptideAtlasiP53811.
PRIDEiP53811.

PTM databases

iPTMnetiP53811.
PhosphoSiteiP53811.
SwissPalmiP53811.

Expressioni

Gene expression databases

BgeeiP53811.
CleanExiMM_PITPNB.
ExpressionAtlasiP53811. baseline and differential.
GenevisibleiP53811. MM.

Interactioni

Protein-protein interaction databases

BioGridi207889. 1 interaction.
IntActiP53811. 2 interactions.
MINTiMINT-4107657.
STRINGi10090.ENSMUSP00000083835.

Structurei

3D structure databases

ProteinModelPortaliP53811.
SMRiP53811. Positions 2-271.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3668. Eukaryota.
COG5083. LUCA.
GeneTreeiENSGT00760000119216.
HOGENOMiHOG000006717.
HOVERGENiHBG058915.
InParanoidiP53811.
OrthoDBiEOG7PGDRB.
PhylomeDBiP53811.
TreeFamiTF313279.

Family and domain databases

Gene3Di3.30.530.20. 1 hit.
InterProiIPR001666. PI_transfer.
IPR023393. START-like_dom.
[Graphical view]
PANTHERiPTHR10658. PTHR10658. 1 hit.
PfamiPF02121. IP_trans. 1 hit.
[Graphical view]
PRINTSiPR00391. PITRANSFER.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLIKEFRVV LPCSVQEYQV GQLYSVAEAS KNETGGGEGI EVLKNEPYEN
60 70 80 90 100
DGEKGQYTHK IYHLKSKVPA FVRMIAPEGS LVFHEKAWNA YPYCRTIVTN
110 120 130 140 150
EYMKDDFFIK IETWHKPDLG TLENVHGLDP NTWKTVEIVH IDIADRSQVE
160 170 180 190 200
PADYKADEDP ALFHSVKTKR GPLGPNWKKE LANTPDCPRM CAYKLVTIKF
210 220 230 240 250
KWWGLQSKVE NFIQKQEKRI FTNLHRQLFC WIDKWIDLTM EDIRRMEDET
260 270
QKELETMRKK GSVRGTSAAD A
Length:271
Mass (Da):31,487
Last modified:January 23, 2007 - v2
Checksum:i9111B615909548CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46934 mRNA. Translation: AAA87593.1.
AK028235 mRNA. Translation: BAC25830.1.
CCDSiCCDS39213.1.
RefSeqiNP_001288573.1. NM_001301644.1.
NP_062614.1. NM_019640.5.
UniGeneiMm.200516.
Mm.474970.

Genome annotation databases

EnsembliENSMUST00000086635; ENSMUSP00000083835; ENSMUSG00000050017.
GeneIDi56305.
KEGGimmu:56305.
UCSCiuc008ysb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46934 mRNA. Translation: AAA87593.1.
AK028235 mRNA. Translation: BAC25830.1.
CCDSiCCDS39213.1.
RefSeqiNP_001288573.1. NM_001301644.1.
NP_062614.1. NM_019640.5.
UniGeneiMm.200516.
Mm.474970.

3D structure databases

ProteinModelPortaliP53811.
SMRiP53811. Positions 2-271.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207889. 1 interaction.
IntActiP53811. 2 interactions.
MINTiMINT-4107657.
STRINGi10090.ENSMUSP00000083835.

PTM databases

iPTMnetiP53811.
PhosphoSiteiP53811.
SwissPalmiP53811.

Proteomic databases

EPDiP53811.
MaxQBiP53811.
PaxDbiP53811.
PeptideAtlasiP53811.
PRIDEiP53811.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000086635; ENSMUSP00000083835; ENSMUSG00000050017.
GeneIDi56305.
KEGGimmu:56305.
UCSCiuc008ysb.2. mouse.

Organism-specific databases

CTDi23760.
MGIiMGI:1927542. Pitpnb.

Phylogenomic databases

eggNOGiKOG3668. Eukaryota.
COG5083. LUCA.
GeneTreeiENSGT00760000119216.
HOGENOMiHOG000006717.
HOVERGENiHBG058915.
InParanoidiP53811.
OrthoDBiEOG7PGDRB.
PhylomeDBiP53811.
TreeFamiTF313279.

Enzyme and pathway databases

ReactomeiR-MMU-1483196. PI and PC transport between ER and Golgi membranes.

Miscellaneous databases

PROiP53811.
SOURCEiSearch...

Gene expression databases

BgeeiP53811.
CleanExiMM_PITPNB.
ExpressionAtlasiP53811. baseline and differential.
GenevisibleiP53811. MM.

Family and domain databases

Gene3Di3.30.530.20. 1 hit.
InterProiIPR001666. PI_transfer.
IPR023393. START-like_dom.
[Graphical view]
PANTHERiPTHR10658. PTHR10658. 1 hit.
PfamiPF02121. IP_trans. 1 hit.
[Graphical view]
PRINTSiPR00391. PITRANSFER.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Geijtenbeek T.B.H., der Helm H.A., Snoek G.T., Wirtz K.W.A.
    Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: SWR/J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  3. "The Golgi localization of phosphatidylinositol transfer protein beta requires the protein kinase C-dependent phosphorylation of serine 262 and is essential for maintaining plasma membrane sphingomyelin levels."
    van Tiel C.M., Westerman J., Paasman M.A., Hoebens M.M., Wirtz K.W., Snoek G.T.
    J. Biol. Chem. 277:22447-22452(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-262, MUTAGENESIS OF SER-165 AND SER-262, IDENTIFICATION BY MASS SPECTROMETRY.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas and Spleen.

Entry informationi

Entry nameiPIPNB_MOUSE
AccessioniPrimary (citable) accession number: P53811
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.