Reviewed, UniProtKB/Swiss-Prot P53810 (PIPNA_MOUSE)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol transfer protein alpha isoform
      Short name=PtdIns transfer protein alpha
      Short name=PtdInsTP
      Short name=PI-TP-alpha

Gene names

Name:	Pitpna
Synonyms:	Pitpn

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the transfer of PtdIns and phosphatidylcholine between membranes.
Subcellular location	Cytoplasm.
Involvement in disease	Defects in Pitpna are the cause of the vibrator phenotype which is characterized by early-onset progressive action tremor, degeneration of brain stem and spinal cord neurons, and juvenile death. The mutation is due to the insertion of an intracisternal A particle retrotransposon in intron 4 which results in a 5-fold reduction in protein levels. Ref.2
Sequence similarities	Belongs to the PtdIns transfer protein family. Class I subfamily.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cytoplasm
Ligand	Lipid-binding
PTM	Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 271

270

Phosphatidylinositol transfer protein alpha isoform

PRO_0000191640

Amino acid modifications

Modified residue

58

1

Phosphotyrosine By similarity

Secondary structure

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271

Helix Strand Turn

Details...

Beta strand

3 – 13

11

Helix

15 – 30

16

Turn

36 – 39

4

Beta strand

40 – 49

10

Beta strand

55 – 64

10

Helix

66 – 68

3

Helix

71 – 74

4

Turn

79 – 82

4

Beta strand

83 – 91

9

Beta strand

94 – 101

8

Turn

102 – 104

3

Helix

105 – 107

3

Beta strand

108 – 121

14

Helix

131 – 134

4

Beta strand

138 – 142

5

Helix

147 – 149

3

Helix

152 – 154

3

Helix

157 – 159

3

Turn

161 – 163

3

Turn

167 – 169

3

Helix

178 – 182

5

Beta strand

191 – 201

11

Helix

207 – 231

25

Helix

233 – 236

4

Helix

241 – 253

13

Sequences

	Sequence		Length	Mass (Da)
	P53810-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: E9561EE5BBBADC3A	FASTA	271	31,893
	10 20 30 40 50 60 MVLLKEYRVI LPVSVDEYQV GQLYSVAEAS KNETGGGEGV EVLVNEPYEK DDGEKGQYTH 70 80 90 100 110 120 KIYHLQSKVP TFVRMLAPEG ALNIHEKAWN AYPYCRTVIT NEYMKEDFLI KIETWHKPDL 130 140 150 160 170 180 GTQENVHKLE PEAWKHVEAI YIDIADRSQV LSKDYKAEED PAKFKSVKTG RGPLGPNWKQ 190 200 210 220 230 240 ELVNQKDCPY MCAYKLVTVK FKWWGLQNKV ENFIHKQEKR LFTNFHRQLF CWLDKWVDLT 250 260 270 MDDIRRMEEE TKRQLDEMRQ KDPVKGMTAD D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of mouse phosphatidylinositol transfer protein expressed in Escherichia coli." Geijtenbeek T.B.H., de Groot E., van Baal J., Brunink F., Westerman J., Snoek G.T., Wirtz K.W. Biochim. Biophys. Acta 1213:309-318(1994) [PubMed: 8049244] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Swiss.
[2]	"The vibrator mutation causes neurodegeneration via reduced expression of PITP alpha: positional complementation cloning and extragenic suppression." Hamilton B.A., Smith D.J., Mueller K.L., Kerrebrock A.W., Bronson R.T., van Berkel V., Daly M.J., Kruglyak L., Reeve M.P., Nemhauser J.L., Hawkins T.L., Rubin E.M., Lander E.S. Neuron 18:711-722(1997) [PubMed: 9182797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], DISEASE. Strain: DBA/2J. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[4]	Lubec G., Klug S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 9-50; 88-96; 112-128 AND 136-147, MASS SPECTROMETRY. Tissue: Hippocampus.
[5]	"Structure of apo-phosphatidylinositol transfer protein alpha provides insight into membrane association." Schouten A., Agianian B., Westerman J., Kroon J., Wirtz K.W., Gros P. EMBO J. 21:2117-2121(2002) [PubMed: 11980708] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

S72681 mRNA. Translation: AAC60690.1.
U96725 mRNA. Translation: AAC53266.1.
U96726 Genomic DNA. Translation: AAC60756.1.
BC056171 mRNA. Translation: AAH56171.1.

IPI

IPI00230003.

RefSeq

NP_032876.1.

UniGene

Mm.3128

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KCM	X-ray	2.00	A	2-271	[»]

ModBase

Search...

PTM databases

PhosphoSite

P53810.

2-D gel databases

REPRODUCTION-2DPAGE

P53810.

Proteomic databases

PRIDE

P53810.

Genome annotation databases

Ensembl

ENSMUSG00000017781. Mus musculus. [Contig view]

GeneID

18738.

KEGG

mmu:18738.

Organism-specific databases

MGI

MGI:99887. Pitpna.

Phylogenomic databases

HOVERGEN

P53810.

OMA

P53810. QSKVPTF.

Gene expression databases

ArrayExpress

P53810.

Bgee

P53810.

CleanEx

MM_PITPNA.

GermOnline

ENSMUSG00000017781. Mus musculus.

Family and domain databases

InterPro

IPR001666. PI_transfer.
[Graphical view]

PANTHER

PTHR10658. PI_transfer. 1 hit.

Pfam

PF02121. IP_trans. 1 hit.
[Graphical view]

PRINTS

PR00391. PITRANSFER.

ProtoNet

Search...

Other Resources

NextBio

294873.

SOURCE

Search...

Entry information

Entry name

PIPNA_MOUSE

Accession

Primary (citable) accession number: P53810

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)