P53808 (PPCT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylcholine transfer protein
Short name=PC-TP

Alternative name(s):
START domain-containing protein 2
Short name=StARD2
StAR-related lipid transfer protein 2

Gene names

Name:	Pctp
Synonyms:	Stard2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the transfer of phosphatidylcholine between membranes. Binds a single lipid molecule.
Subunit structure	Interacts with ACOT13/THEM2. Ref.4
Subcellular location	Cytoplasm Ref.1.
Tissue specificity	Abundant in liver of pups but levels in liver decrease 10-fold about 2 weeks after birth. In adult, highly expressed in epididymis, testis, kidney and bone-marrow derived mast cells. Ref.1
Sequence similarities	Contains 1 START domain.

Ontologies

Keywords
Biological process	Lipid transport Transport
Cellular component	Cytoplasm
Ligand	Lipid-binding
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cholesterol metabolic process Inferred from mutant phenotype PubMed 15845870. Source: MGI
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphatidylcholine binding Inferred from sequence or structural similarity. Source: UniProtKB phosphatidylcholine transporter activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 214	214	Phosphatidylcholine transfer protein		PRO_0000220659
Regions
	Domain	1 – 212	212	START
Sites
	Binding site	72	1	Phosphatidylcholine By similarity
	Binding site	78	1	Phosphatidylcholine By similarity
	Binding site	157	1	Phosphatidylcholine By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
Experimental info
	Sequence conflict	90	1	E → D in AAF08346. Ref.3
	Sequence conflict	127	1	R → G in AAF08346. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P53808 [UniParc]. Last modified August 14, 2001. Version 2. Checksum: 8BE2B046314DAE70	FASTA	214	24,785
	10 20 30 40 50 60 MAGAACCFSD EQFREACAEL QKPALTGADW QLLVEASGIT IYRLLDQPSG LYEYKVFGVL 70 80 90 100 110 120 EGCSPALLTD VYMDLDYRKQ WDQYVKELYE KESDEQMVAY WEVKYPFPLS NRDYVYTRQR 130 140 150 160 170 180 RDLDVDRRKI YVVLAQSISA PQFPEKSGVI RVKQYKQSLA IESDGKKGSR VFMYYFDNPG 190 200 210 GQIPSWLINW AAKNGVPNFL KDMVKACQNY HKKT

References

[1]	"Mice without phosphatidylcholine transfer protein have no defects in the secretion of PC into bile or into the lung airspaces." van Helvoort A., de Brouwer A., Ottenhoff R., Brouwers J.F.H.M., Wijnholds J., Beijnen J.H., Rijneveld A., van der Poll T., van der Valk M.A., Majoor D., Voorhout W., Wirtz K.W.A., Oude Elferink R.P.J., Borst P. Proc. Natl. Acad. Sci. U.S.A. 96:11501-11506(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: 129/Ola. Tissue: Liver.
[2]	"cDNA cloning and tissue-specific expression of the phosphatidylcholine transfer protein gene." Geijtenbeek T.B.H., Smith A.J., Borst P., Wirtz K.W.A. Biochem. J. 316:49-55(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 41-214. Tissue: Lung.
[3]	"Cloning, tissue-specific expression, gene structure and chromosomal localization of human phosphatidylcholine transfer protein." Cohen D.E., Green R.M., Wu M.K., Beier D.R. Biochim. Biophys. Acta 1447:265-270(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 82-214. Strain: C57BL/6 X CBA. Tissue: Liver.
[4]	"Interacting proteins dictate function of the minimal START domain phosphatidylcholine transfer protein/StarD2." Kanno K., Wu M.K., Agate D.S., Fanelli B.J., Wagle N., Scapa E.F., Ukomadu C., Cohen D.E. J. Biol. Chem. 282:30728-30736(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ACOT13/THEM2 AND PAX3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF151639 mRNA. Translation: AAF02537.1. Z50024 mRNA. Translation: CAA90328.1. AF114437 mRNA. Translation: AAF08346.1.
IPI	IPI00135725.
PIR	S68246.
RefSeq	NP_032822.2. NM_008796.2.
UniGene	Mm.5062.
3D structure databases
ProteinModelPortal	P53808.
SMR	P53808. Positions 8-210.
ModBase	Search...
PTM databases
PhosphoSite	P53808.
Proteomic databases
PaxDb	P53808.
PRIDE	P53808.
Protocols and materials databases
DNASU	18559.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	18559.
KEGG	mmu:18559.
Organism-specific databases
CTD	58488.
MGI	MGI:107375. Pctp.
Phylogenomic databases
eggNOG	NOG264966.
HOGENOM	HOG000261644.
HOVERGEN	HBG008237.
InParanoid	P53808.
OrthoDB	EOG47SSFK.
Enzyme and pathway databases
SABIO-RK	P53808.
Gene expression databases
CleanEx	MM_PCTP.
Genevestigator	P53808.
GermOnline	ENSMUSG00000020553. Mus musculus.
Family and domain databases
Gene3D	3.30.530.20. 1 hit.
InterPro	IPR023393. START-like_dom. IPR002913. START_lipid-bd_dom. [Graphical view]
Pfam	PF01852. START. 1 hit. [Graphical view]
SMART	SM00234. START. 1 hit. [Graphical view]
PROSITE	PS50848. START. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	294384.
SOURCE	Search...

Entry information

Entry name

PPCT_MOUSE

Accession

Primary (citable) accession number: P53808
Secondary accession number(s): Q9QZX0, Q9R058

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	August 14, 2001
Last modified:	April 3, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program