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P53803

- RPAB4_HUMAN

UniProt

P53803 - RPAB4_HUMAN

Protein

DNA-directed RNA polymerases I, II, and III subunit RPABC4

Gene

POLR2K

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi19 – 191ZincBy similarity
    Metal bindingi22 – 221ZincBy similarity
    Metal bindingi36 – 361ZincBy similarity
    Metal bindingi39 – 391ZincBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri19 – 3921C4-typeSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. DNA-directed RNA polymerase activity Source: UniProtKB-KW
    3. zinc ion binding Source: ProtInc

    GO - Biological processi

    1. 7-methylguanosine mRNA capping Source: Reactome
    2. DNA repair Source: Reactome
    3. gene expression Source: Reactome
    4. innate immune response Source: Reactome
    5. mRNA splicing, via spliceosome Source: Reactome
    6. nucleotide-excision repair Source: Reactome
    7. positive regulation of type I interferon production Source: Reactome
    8. positive regulation of viral transcription Source: Reactome
    9. regulation of transcription from RNA polymerase I promoter Source: ProtInc
    10. RNA splicing Source: Reactome
    11. termination of RNA polymerase III transcription Source: Reactome
    12. termination of RNA polymerase I transcription Source: Reactome
    13. transcription-coupled nucleotide-excision repair Source: Reactome
    14. transcription elongation from RNA polymerase III promoter Source: Reactome
    15. transcription elongation from RNA polymerase II promoter Source: Reactome
    16. transcription elongation from RNA polymerase I promoter Source: Reactome
    17. transcription from RNA polymerase III promoter Source: Reactome
    18. transcription from RNA polymerase II promoter Source: UniProtKB
    19. transcription from RNA polymerase I promoter Source: Reactome
    20. transcription initiation from RNA polymerase II promoter Source: Reactome
    21. transcription initiation from RNA polymerase I promoter Source: Reactome
    22. viral process Source: Reactome

    Keywords - Biological processi

    Transcription

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_1036. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
    REACT_1074. RNA Polymerase I Transcription Termination.
    REACT_12417. MicroRNA (miRNA) biogenesis.
    REACT_125. Processing of Capped Intron-Containing Pre-mRNA.
    REACT_1470. mRNA Capping.
    REACT_1628. Transcription-coupled NER (TC-NER).
    REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
    REACT_1753. mRNA Splicing - Minor Pathway.
    REACT_1851. RNA Polymerase II Transcription Initiation.
    REACT_1913. RNA Polymerase I Promoter Escape.
    REACT_1941. Formation of transcription-coupled NER (TC-NER) repair complex.
    REACT_200856. NoRC negatively regulates rRNA expression.
    REACT_2089. RNA Polymerase II Promoter Escape.
    REACT_2204. RNA Polymerase I Chain Elongation.
    REACT_22107. RNA Polymerase II Pre-transcription Events.
    REACT_22201. Formation of HIV elongation complex in the absence of HIV Tat.
    REACT_2222. Dual incision reaction in TC-NER.
    REACT_22339. RNA Polymerase III Abortive And Retractive Initiation.
    REACT_347. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
    REACT_467. mRNA Splicing - Major Pathway.
    REACT_571. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
    REACT_6143. Pausing and recovery of Tat-mediated HIV elongation.
    REACT_6162. Tat-mediated elongation of the HIV-1 transcript.
    REACT_6233. Transcription of the HIV genome.
    REACT_6237. RNA Pol II CTD phosphorylation and interaction with CE.
    REACT_6244. Pausing and recovery of HIV elongation.
    REACT_6253. RNA Polymerase II HIV Promoter Escape.
    REACT_6259. HIV elongation arrest and recovery.
    REACT_6261. Abortive elongation of HIV-1 transcript in the absence of Tat.
    REACT_63. RNA Polymerase III Transcription Termination.
    REACT_6319. Formation of the HIV-1 Early Elongation Complex.
    REACT_6332. HIV Transcription Initiation.
    REACT_6344. Tat-mediated HIV elongation arrest and recovery.
    REACT_6346. Formation of HIV-1 elongation complex containing HIV-1 Tat.
    REACT_6354. Viral Messenger RNA Synthesis.
    REACT_756. RNA Polymerase III Chain Elongation.
    REACT_833. RNA Polymerase II Transcription Elongation.
    REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.
    REACT_846. Formation of the Early Elongation Complex.
    REACT_953. RNA Polymerase I Transcription Initiation.
    REACT_975. RNA Pol II CTD phosphorylation and interaction with CE.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-directed RNA polymerases I, II, and III subunit RPABC4
    Short name:
    RNA polymerases I, II, and III subunit ABC4
    Alternative name(s):
    ABC10-alpha
    DNA-directed RNA polymerase II subunit K
    RNA polymerase II 7.0 kDa subunit
    Short name:
    RPB7.0
    RPB10alpha
    Gene namesi
    Name:POLR2K
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:9198. POLR2K.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. DNA-directed RNA polymerase I complex Source: RefGenome
    3. DNA-directed RNA polymerase II, core complex Source: UniProtKB
    4. DNA-directed RNA polymerase III complex Source: RefGenome
    5. nucleoplasm Source: Reactome
    6. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    DNA-directed RNA polymerase, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33518.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 5858DNA-directed RNA polymerases I, II, and III subunit RPABC4PRO_0000159750Add
    BLAST

    Proteomic databases

    MaxQBiP53803.
    PRIDEiP53803.

    Expressioni

    Gene expression databases

    ArrayExpressiP53803.
    BgeeiP53803.
    CleanExiHS_POLR2K.
    GenevestigatoriP53803.

    Interactioni

    Subunit structurei

    Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively.By similarity

    Protein-protein interaction databases

    BioGridi111436. 15 interactions.
    IntActiP53803. 2 interactions.
    STRINGi9606.ENSP00000342889.

    Structurei

    3D structure databases

    ProteinModelPortaliP53803.
    SMRiP53803. Positions 15-58.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri19 – 3921C4-typeSequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG1996.
    HOVERGENiHBG054239.
    InParanoidiP53803.
    KOiK03009.
    OMAiPRQDMEY.
    OrthoDBiEOG73540T.
    PhylomeDBiP53803.
    TreeFamiTF103045.

    Family and domain databases

    Gene3Di2.20.28.30. 1 hit.
    InterProiIPR006591. RNAP_P/RPABC4.
    IPR029040. RPABC4/Spt4.
    [Graphical view]
    PfamiPF03604. DNA_RNApol_7kD. 1 hit.
    [Graphical view]
    SMARTiSM00659. RPOLCX. 1 hit.
    [Graphical view]
    SUPFAMiSSF63393. SSF63393. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P53803-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDTQKDVQPP KQQPMIYICG ECHTENEIKS RDPIRCRECG YRIMYKKRTK   50
    RLVVFDAR 58
    Length:58
    Mass (Da):7,004
    Last modified:October 1, 1996 - v1
    Checksum:i239BA3A67416F02C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z47727 mRNA. Translation: CAA87656.1.
    AJ252078 Genomic DNA. Translation: CAB91873.1.
    CR456870 mRNA. Translation: CAG33151.1.
    CH471060 Genomic DNA. Translation: EAW91798.1.
    BC000806 mRNA. Translation: AAH00806.1.
    BC018157 mRNA. Translation: AAH18157.1.
    CCDSiCCDS6285.1.
    PIRiI37558.
    RefSeqiNP_005025.1. NM_005034.3.
    UniGeneiHs.351475.

    Genome annotation databases

    EnsembliENST00000353107; ENSP00000342889; ENSG00000147669.
    GeneIDi5440.
    KEGGihsa:5440.
    UCSCiuc003yjf.3. human.

    Polymorphism databases

    DMDMi1710664.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z47727 mRNA. Translation: CAA87656.1 .
    AJ252078 Genomic DNA. Translation: CAB91873.1 .
    CR456870 mRNA. Translation: CAG33151.1 .
    CH471060 Genomic DNA. Translation: EAW91798.1 .
    BC000806 mRNA. Translation: AAH00806.1 .
    BC018157 mRNA. Translation: AAH18157.1 .
    CCDSi CCDS6285.1.
    PIRi I37558.
    RefSeqi NP_005025.1. NM_005034.3.
    UniGenei Hs.351475.

    3D structure databases

    ProteinModelPortali P53803.
    SMRi P53803. Positions 15-58.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111436. 15 interactions.
    IntActi P53803. 2 interactions.
    STRINGi 9606.ENSP00000342889.

    Polymorphism databases

    DMDMi 1710664.

    Proteomic databases

    MaxQBi P53803.
    PRIDEi P53803.

    Protocols and materials databases

    DNASUi 5440.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000353107 ; ENSP00000342889 ; ENSG00000147669 .
    GeneIDi 5440.
    KEGGi hsa:5440.
    UCSCi uc003yjf.3. human.

    Organism-specific databases

    CTDi 5440.
    GeneCardsi GC08P101162.
    HGNCi HGNC:9198. POLR2K.
    MIMi 606033. gene.
    neXtProti NX_P53803.
    PharmGKBi PA33518.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1996.
    HOVERGENi HBG054239.
    InParanoidi P53803.
    KOi K03009.
    OMAi PRQDMEY.
    OrthoDBi EOG73540T.
    PhylomeDBi P53803.
    TreeFami TF103045.

    Enzyme and pathway databases

    Reactomei REACT_1036. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
    REACT_1074. RNA Polymerase I Transcription Termination.
    REACT_12417. MicroRNA (miRNA) biogenesis.
    REACT_125. Processing of Capped Intron-Containing Pre-mRNA.
    REACT_1470. mRNA Capping.
    REACT_1628. Transcription-coupled NER (TC-NER).
    REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
    REACT_1753. mRNA Splicing - Minor Pathway.
    REACT_1851. RNA Polymerase II Transcription Initiation.
    REACT_1913. RNA Polymerase I Promoter Escape.
    REACT_1941. Formation of transcription-coupled NER (TC-NER) repair complex.
    REACT_200856. NoRC negatively regulates rRNA expression.
    REACT_2089. RNA Polymerase II Promoter Escape.
    REACT_2204. RNA Polymerase I Chain Elongation.
    REACT_22107. RNA Polymerase II Pre-transcription Events.
    REACT_22201. Formation of HIV elongation complex in the absence of HIV Tat.
    REACT_2222. Dual incision reaction in TC-NER.
    REACT_22339. RNA Polymerase III Abortive And Retractive Initiation.
    REACT_347. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
    REACT_467. mRNA Splicing - Major Pathway.
    REACT_571. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
    REACT_6143. Pausing and recovery of Tat-mediated HIV elongation.
    REACT_6162. Tat-mediated elongation of the HIV-1 transcript.
    REACT_6233. Transcription of the HIV genome.
    REACT_6237. RNA Pol II CTD phosphorylation and interaction with CE.
    REACT_6244. Pausing and recovery of HIV elongation.
    REACT_6253. RNA Polymerase II HIV Promoter Escape.
    REACT_6259. HIV elongation arrest and recovery.
    REACT_6261. Abortive elongation of HIV-1 transcript in the absence of Tat.
    REACT_63. RNA Polymerase III Transcription Termination.
    REACT_6319. Formation of the HIV-1 Early Elongation Complex.
    REACT_6332. HIV Transcription Initiation.
    REACT_6344. Tat-mediated HIV elongation arrest and recovery.
    REACT_6346. Formation of HIV-1 elongation complex containing HIV-1 Tat.
    REACT_6354. Viral Messenger RNA Synthesis.
    REACT_756. RNA Polymerase III Chain Elongation.
    REACT_833. RNA Polymerase II Transcription Elongation.
    REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.
    REACT_846. Formation of the Early Elongation Complex.
    REACT_953. RNA Polymerase I Transcription Initiation.
    REACT_975. RNA Pol II CTD phosphorylation and interaction with CE.

    Miscellaneous databases

    GeneWikii POLR2K.
    GenomeRNAii 5440.
    NextBioi 21051.
    PROi P53803.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P53803.
    Bgeei P53803.
    CleanExi HS_POLR2K.
    Genevestigatori P53803.

    Family and domain databases

    Gene3Di 2.20.28.30. 1 hit.
    InterProi IPR006591. RNAP_P/RPABC4.
    IPR029040. RPABC4/Spt4.
    [Graphical view ]
    Pfami PF03604. DNA_RNApol_7kD. 1 hit.
    [Graphical view ]
    SMARTi SM00659. RPOLCX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF63393. SSF63393. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Four subunits that are shared by the three classes of RNA polymerase are functionally interchangeable between Homo sapiens and Saccharomyces cerevisiae."
      Shpakovski G.V., Acker J., Wintzerith M., Lacroix J.F., Thuriaux P., Vigneron M.
      Mol. Cell. Biol. 15:4702-4710(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Organization of genes encoding subunits of eucaryotic nuclear RNA polymerases shows non random intron distribution and correlates with the subunit modular structure."
      Shpakovski G.V., Lebedenko E.N., Grandemange S., Schaller S., Vigneron M., Kedinger C.
      Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Placenta.
    3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Ovary and Placenta.

    Entry informationi

    Entry nameiRPAB4_HUMAN
    AccessioniPrimary (citable) accession number: P53803
    Secondary accession number(s): Q6IBD4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3