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Protein

DNA-directed RNA polymerases I, II, and III subunit RPABC4

Gene

POLR2K

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi19 – 191ZincBy similarity
Metal bindingi22 – 221ZincBy similarity
Metal bindingi36 – 361ZincBy similarity
Metal bindingi39 – 391ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri19 – 3921C4-typeSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. DNA-directed RNA polymerase activity Source: UniProtKB-KW
  3. zinc ion binding Source: ProtInc

GO - Biological processi

  1. 7-methylguanosine mRNA capping Source: Reactome
  2. DNA repair Source: Reactome
  3. gene expression Source: Reactome
  4. innate immune response Source: Reactome
  5. mRNA splicing, via spliceosome Source: Reactome
  6. negative regulation of gene expression, epigenetic Source: Reactome
  7. nucleotide-excision repair Source: Reactome
  8. piRNA metabolic process Source: Reactome
  9. positive regulation of type I interferon production Source: Reactome
  10. positive regulation of viral transcription Source: Reactome
  11. regulation of gene expression, epigenetic Source: Reactome
  12. regulation of transcription from RNA polymerase I promoter Source: ProtInc
  13. RNA splicing Source: Reactome
  14. somatic stem cell maintenance Source: Reactome
  15. termination of RNA polymerase III transcription Source: Reactome
  16. termination of RNA polymerase I transcription Source: Reactome
  17. transcription-coupled nucleotide-excision repair Source: Reactome
  18. transcription elongation from RNA polymerase III promoter Source: Reactome
  19. transcription elongation from RNA polymerase II promoter Source: Reactome
  20. transcription elongation from RNA polymerase I promoter Source: Reactome
  21. transcription from RNA polymerase III promoter Source: Reactome
  22. transcription from RNA polymerase II promoter Source: UniProtKB
  23. transcription from RNA polymerase I promoter Source: Reactome
  24. transcription initiation from RNA polymerase II promoter Source: Reactome
  25. transcription initiation from RNA polymerase I promoter Source: Reactome
  26. viral process Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Transcription

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_1036. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
REACT_1074. RNA Polymerase I Transcription Termination.
REACT_118823. Cytosolic sensors of pathogen-associated DNA.
REACT_12417. MicroRNA (miRNA) biogenesis.
REACT_125. Processing of Capped Intron-Containing Pre-mRNA.
REACT_1470. mRNA Capping.
REACT_1628. Transcription-coupled NER (TC-NER).
REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_1753. mRNA Splicing - Minor Pathway.
REACT_1845. Formation of RNA Pol II elongation complex.
REACT_1851. RNA Polymerase II Transcription Initiation.
REACT_1913. RNA Polymerase I Promoter Escape.
REACT_1941. Formation of transcription-coupled NER (TC-NER) repair complex.
REACT_2089. RNA Polymerase II Promoter Escape.
REACT_2204. RNA Polymerase I Chain Elongation.
REACT_22107. RNA Polymerase II Pre-transcription Events.
REACT_22201. Formation of HIV elongation complex in the absence of HIV Tat.
REACT_2222. Dual incision reaction in TC-NER.
REACT_22339. RNA Polymerase III Abortive And Retractive Initiation.
REACT_263856. Transcriptional regulation of pluripotent stem cells.
REACT_263965. NoRC negatively regulates rRNA expression.
REACT_264617. POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation.
REACT_267790. PIWI-interacting RNA (piRNA) biogenesis.
REACT_268530. Transcriptional regulation by small RNAs.
REACT_347. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
REACT_467. mRNA Splicing - Major Pathway.
REACT_571. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
REACT_6143. Pausing and recovery of Tat-mediated HIV elongation.
REACT_6162. Tat-mediated elongation of the HIV-1 transcript.
REACT_6233. Transcription of the HIV genome.
REACT_6237. RNA Pol II CTD phosphorylation and interaction with CE.
REACT_6244. Pausing and recovery of HIV elongation.
REACT_6253. RNA Polymerase II HIV Promoter Escape.
REACT_6259. HIV elongation arrest and recovery.
REACT_6261. Abortive elongation of HIV-1 transcript in the absence of Tat.
REACT_63. RNA Polymerase III Transcription Termination.
REACT_6319. Formation of the HIV-1 Early Elongation Complex.
REACT_6332. HIV Transcription Initiation.
REACT_6344. Tat-mediated HIV elongation arrest and recovery.
REACT_6346. Formation of HIV-1 elongation complex containing HIV-1 Tat.
REACT_6354. Viral Messenger RNA Synthesis.
REACT_756. RNA Polymerase III Chain Elongation.
REACT_833. RNA Polymerase II Transcription Elongation.
REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_846. Formation of the Early Elongation Complex.
REACT_953. RNA Polymerase I Transcription Initiation.
REACT_975. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerases I, II, and III subunit RPABC4
Short name:
RNA polymerases I, II, and III subunit ABC4
Alternative name(s):
ABC10-alpha
DNA-directed RNA polymerase II subunit K
RNA polymerase II 7.0 kDa subunit
Short name:
RPB7.0
RPB10alpha
Gene namesi
Name:POLR2K
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:9198. POLR2K.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. DNA-directed RNA polymerase I complex Source: GO_Central
  3. DNA-directed RNA polymerase II, core complex Source: UniProtKB
  4. DNA-directed RNA polymerase III complex Source: GO_Central
  5. nucleoplasm Source: Reactome
  6. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33518.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5858DNA-directed RNA polymerases I, II, and III subunit RPABC4PRO_0000159750Add
BLAST

Proteomic databases

MaxQBiP53803.
PRIDEiP53803.

Expressioni

Gene expression databases

BgeeiP53803.
CleanExiHS_POLR2K.
ExpressionAtlasiP53803. baseline and differential.
GenevestigatoriP53803.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively.By similarity

Protein-protein interaction databases

BioGridi111436. 20 interactions.
IntActiP53803. 2 interactions.
STRINGi9606.ENSP00000342889.

Structurei

3D structure databases

ProteinModelPortaliP53803.
SMRiP53803. Positions 15-58.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri19 – 3921C4-typeSequence AnalysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG1996.
GeneTreeiENSGT00390000008918.
HOVERGENiHBG054239.
InParanoidiP53803.
KOiK03009.
OMAiPRQDMEY.
OrthoDBiEOG73540T.
PhylomeDBiP53803.
TreeFamiTF103045.

Family and domain databases

Gene3Di2.20.28.30. 1 hit.
InterProiIPR006591. RNAP_P/RPABC4.
IPR029040. RPABC4/Spt4.
[Graphical view]
PfamiPF03604. DNA_RNApol_7kD. 1 hit.
[Graphical view]
SMARTiSM00659. RPOLCX. 1 hit.
[Graphical view]
SUPFAMiSSF63393. SSF63393. 1 hit.

Sequencei

Sequence statusi: Complete.

P53803-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTQKDVQPP KQQPMIYICG ECHTENEIKS RDPIRCRECG YRIMYKKRTK

RLVVFDAR
Length:58
Mass (Da):7,004
Last modified:October 1, 1996 - v1
Checksum:i239BA3A67416F02C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47727 mRNA. Translation: CAA87656.1.
AJ252078 Genomic DNA. Translation: CAB91873.1.
CR456870 mRNA. Translation: CAG33151.1.
CH471060 Genomic DNA. Translation: EAW91798.1.
BC000806 mRNA. Translation: AAH00806.1.
BC018157 mRNA. Translation: AAH18157.1.
CCDSiCCDS6285.1.
PIRiI37558.
RefSeqiNP_005025.1. NM_005034.3.
UniGeneiHs.351475.

Genome annotation databases

EnsembliENST00000353107; ENSP00000342889; ENSG00000147669.
GeneIDi5440.
KEGGihsa:5440.
UCSCiuc003yjf.3. human.

Polymorphism databases

DMDMi1710664.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47727 mRNA. Translation: CAA87656.1.
AJ252078 Genomic DNA. Translation: CAB91873.1.
CR456870 mRNA. Translation: CAG33151.1.
CH471060 Genomic DNA. Translation: EAW91798.1.
BC000806 mRNA. Translation: AAH00806.1.
BC018157 mRNA. Translation: AAH18157.1.
CCDSiCCDS6285.1.
PIRiI37558.
RefSeqiNP_005025.1. NM_005034.3.
UniGeneiHs.351475.

3D structure databases

ProteinModelPortaliP53803.
SMRiP53803. Positions 15-58.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111436. 20 interactions.
IntActiP53803. 2 interactions.
STRINGi9606.ENSP00000342889.

Polymorphism databases

DMDMi1710664.

Proteomic databases

MaxQBiP53803.
PRIDEiP53803.

Protocols and materials databases

DNASUi5440.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000353107; ENSP00000342889; ENSG00000147669.
GeneIDi5440.
KEGGihsa:5440.
UCSCiuc003yjf.3. human.

Organism-specific databases

CTDi5440.
GeneCardsiGC08P101162.
HGNCiHGNC:9198. POLR2K.
MIMi606033. gene.
neXtProtiNX_P53803.
PharmGKBiPA33518.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1996.
GeneTreeiENSGT00390000008918.
HOVERGENiHBG054239.
InParanoidiP53803.
KOiK03009.
OMAiPRQDMEY.
OrthoDBiEOG73540T.
PhylomeDBiP53803.
TreeFamiTF103045.

Enzyme and pathway databases

ReactomeiREACT_1036. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
REACT_1074. RNA Polymerase I Transcription Termination.
REACT_118823. Cytosolic sensors of pathogen-associated DNA.
REACT_12417. MicroRNA (miRNA) biogenesis.
REACT_125. Processing of Capped Intron-Containing Pre-mRNA.
REACT_1470. mRNA Capping.
REACT_1628. Transcription-coupled NER (TC-NER).
REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_1753. mRNA Splicing - Minor Pathway.
REACT_1845. Formation of RNA Pol II elongation complex.
REACT_1851. RNA Polymerase II Transcription Initiation.
REACT_1913. RNA Polymerase I Promoter Escape.
REACT_1941. Formation of transcription-coupled NER (TC-NER) repair complex.
REACT_2089. RNA Polymerase II Promoter Escape.
REACT_2204. RNA Polymerase I Chain Elongation.
REACT_22107. RNA Polymerase II Pre-transcription Events.
REACT_22201. Formation of HIV elongation complex in the absence of HIV Tat.
REACT_2222. Dual incision reaction in TC-NER.
REACT_22339. RNA Polymerase III Abortive And Retractive Initiation.
REACT_263856. Transcriptional regulation of pluripotent stem cells.
REACT_263965. NoRC negatively regulates rRNA expression.
REACT_264617. POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation.
REACT_267790. PIWI-interacting RNA (piRNA) biogenesis.
REACT_268530. Transcriptional regulation by small RNAs.
REACT_347. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
REACT_467. mRNA Splicing - Major Pathway.
REACT_571. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
REACT_6143. Pausing and recovery of Tat-mediated HIV elongation.
REACT_6162. Tat-mediated elongation of the HIV-1 transcript.
REACT_6233. Transcription of the HIV genome.
REACT_6237. RNA Pol II CTD phosphorylation and interaction with CE.
REACT_6244. Pausing and recovery of HIV elongation.
REACT_6253. RNA Polymerase II HIV Promoter Escape.
REACT_6259. HIV elongation arrest and recovery.
REACT_6261. Abortive elongation of HIV-1 transcript in the absence of Tat.
REACT_63. RNA Polymerase III Transcription Termination.
REACT_6319. Formation of the HIV-1 Early Elongation Complex.
REACT_6332. HIV Transcription Initiation.
REACT_6344. Tat-mediated HIV elongation arrest and recovery.
REACT_6346. Formation of HIV-1 elongation complex containing HIV-1 Tat.
REACT_6354. Viral Messenger RNA Synthesis.
REACT_756. RNA Polymerase III Chain Elongation.
REACT_833. RNA Polymerase II Transcription Elongation.
REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_846. Formation of the Early Elongation Complex.
REACT_953. RNA Polymerase I Transcription Initiation.
REACT_975. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

GeneWikiiPOLR2K.
GenomeRNAii5440.
NextBioi21051.
PROiP53803.
SOURCEiSearch...

Gene expression databases

BgeeiP53803.
CleanExiHS_POLR2K.
ExpressionAtlasiP53803. baseline and differential.
GenevestigatoriP53803.

Family and domain databases

Gene3Di2.20.28.30. 1 hit.
InterProiIPR006591. RNAP_P/RPABC4.
IPR029040. RPABC4/Spt4.
[Graphical view]
PfamiPF03604. DNA_RNApol_7kD. 1 hit.
[Graphical view]
SMARTiSM00659. RPOLCX. 1 hit.
[Graphical view]
SUPFAMiSSF63393. SSF63393. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Four subunits that are shared by the three classes of RNA polymerase are functionally interchangeable between Homo sapiens and Saccharomyces cerevisiae."
    Shpakovski G.V., Acker J., Wintzerith M., Lacroix J.F., Thuriaux P., Vigneron M.
    Mol. Cell. Biol. 15:4702-4710(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Organization of genes encoding subunits of eucaryotic nuclear RNA polymerases shows non random intron distribution and correlates with the subunit modular structure."
    Shpakovski G.V., Lebedenko E.N., Grandemange S., Schaller S., Vigneron M., Kedinger C.
    Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Placenta.
  3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary and Placenta.

Entry informationi

Entry nameiRPAB4_HUMAN
AccessioniPrimary (citable) accession number: P53803
Secondary accession number(s): Q6IBD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 1, 2015
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.