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P53803 (RPAB4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerases I, II, and III subunit RPABC4

Short name=RNA polymerases I, II, and III subunit ABC4
Alternative name(s):
ABC10-alpha
DNA-directed RNA polymerase II subunit K
RNA polymerase II 7.0 kDa subunit
Short name=RPB7.0
RPB10alpha
Gene names
Name:POLR2K
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length58 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively.

Subunit structure

Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the archaeal RpoP/eukaryotic RPC10 RNA polymerase subunit family.

Ontologies

Keywords
   Biological processTranscription
   Cellular componentDNA-directed RNA polymerase
Nucleus
   DomainZinc-finger
   LigandMetal-binding
Zinc
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_process7-methylguanosine mRNA capping

Traceable author statement. Source: Reactome

DNA repair

Traceable author statement. Source: Reactome

RNA splicing

Traceable author statement. Source: Reactome

gene expression

Traceable author statement. Source: Reactome

innate immune response

Traceable author statement. Source: Reactome

mRNA splicing, via spliceosome

Traceable author statement. Source: Reactome

nucleotide-excision repair

Traceable author statement. Source: Reactome

positive regulation of type I interferon production

Traceable author statement. Source: Reactome

positive regulation of viral transcription

Traceable author statement. Source: Reactome

regulation of transcription from RNA polymerase I promoter

Traceable author statement Ref.1. Source: ProtInc

termination of RNA polymerase I transcription

Traceable author statement. Source: Reactome

termination of RNA polymerase III transcription

Traceable author statement. Source: Reactome

transcription elongation from RNA polymerase I promoter

Traceable author statement. Source: Reactome

transcription elongation from RNA polymerase II promoter

Traceable author statement. Source: Reactome

transcription elongation from RNA polymerase III promoter

Traceable author statement. Source: Reactome

transcription from RNA polymerase I promoter

Traceable author statement. Source: Reactome

transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 9852112. Source: UniProtKB

transcription from RNA polymerase III promoter

Traceable author statement. Source: Reactome

transcription initiation from RNA polymerase I promoter

Traceable author statement. Source: Reactome

transcription initiation from RNA polymerase II promoter

Traceable author statement. Source: Reactome

transcription-coupled nucleotide-excision repair

Traceable author statement. Source: Reactome

viral process

Traceable author statement. Source: Reactome

   Cellular_componentDNA-directed RNA polymerase I complex

Inferred from Biological aspect of Ancestor. Source: RefGenome

DNA-directed RNA polymerase II, core complex

Inferred from direct assay PubMed 9852112. Source: UniProtKB

DNA-directed RNA polymerase III complex

Inferred from Biological aspect of Ancestor. Source: RefGenome

cytosol

Traceable author statement. Source: Reactome

nucleoplasm

Traceable author statement. Source: Reactome

nucleus

Inferred from direct assay PubMed 9852112. Source: UniProtKB

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

DNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

zinc ion binding

Traceable author statement Ref.1. Source: ProtInc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5858DNA-directed RNA polymerases I, II, and III subunit RPABC4
PRO_0000159750

Regions

Zinc finger19 – 3921C4-type Potential

Sites

Metal binding191Zinc By similarity
Metal binding221Zinc By similarity
Metal binding361Zinc By similarity
Metal binding391Zinc By similarity

Sequences

Sequence LengthMass (Da)Tools
P53803 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 239BA3A67416F02C

FASTA587,004
        10         20         30         40         50 
MDTQKDVQPP KQQPMIYICG ECHTENEIKS RDPIRCRECG YRIMYKKRTK RLVVFDAR 

« Hide

References

« Hide 'large scale' references
[1]"Four subunits that are shared by the three classes of RNA polymerase are functionally interchangeable between Homo sapiens and Saccharomyces cerevisiae."
Shpakovski G.V., Acker J., Wintzerith M., Lacroix J.F., Thuriaux P., Vigneron M.
Mol. Cell. Biol. 15:4702-4710(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Organization of genes encoding subunits of eucaryotic nuclear RNA polymerases shows non random intron distribution and correlates with the subunit modular structure."
Shpakovski G.V., Lebedenko E.N., Grandemange S., Schaller S., Vigneron M., Kedinger C.
Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Placenta.
[3]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary and Placenta.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z47727 mRNA. Translation: CAA87656.1.
AJ252078 Genomic DNA. Translation: CAB91873.1.
CR456870 mRNA. Translation: CAG33151.1.
CH471060 Genomic DNA. Translation: EAW91798.1.
BC000806 mRNA. Translation: AAH00806.1.
BC018157 mRNA. Translation: AAH18157.1.
CCDSCCDS6285.1.
PIRI37558.
RefSeqNP_005025.1. NM_005034.3.
UniGeneHs.351475.

3D structure databases

ProteinModelPortalP53803.
SMRP53803. Positions 15-58.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111436. 15 interactions.
IntActP53803. 2 interactions.
STRING9606.ENSP00000342889.

Polymorphism databases

DMDM1710664.

Proteomic databases

MaxQBP53803.
PRIDEP53803.

Protocols and materials databases

DNASU5440.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000353107; ENSP00000342889; ENSG00000147669.
GeneID5440.
KEGGhsa:5440.
UCSCuc003yjf.3. human.

Organism-specific databases

CTD5440.
GeneCardsGC08P101162.
HGNCHGNC:9198. POLR2K.
MIM606033. gene.
neXtProtNX_P53803.
PharmGKBPA33518.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1996.
HOVERGENHBG054239.
InParanoidP53803.
KOK03009.
OMAPRQDMEY.
OrthoDBEOG73540T.
PhylomeDBP53803.
TreeFamTF103045.

Enzyme and pathway databases

ReactomeREACT_116125. Disease.
REACT_1788. Transcription.
REACT_1892. Elongation arrest and recovery.
REACT_216. DNA Repair.
REACT_6900. Immune System.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpressP53803.
BgeeP53803.
CleanExHS_POLR2K.
GenevestigatorP53803.

Family and domain databases

Gene3D2.20.28.30. 1 hit.
InterProIPR006591. RNAP_P/RPABC4.
IPR029040. RPABC4/Spt4.
[Graphical view]
PfamPF03604. DNA_RNApol_7kD. 1 hit.
[Graphical view]
SMARTSM00659. RPOLCX. 1 hit.
[Graphical view]
SUPFAMSSF63393. SSF63393. 1 hit.
ProtoNetSearch...

Other

GeneWikiPOLR2K.
GenomeRNAi5440.
NextBio21051.
PROP53803.
SOURCESearch...

Entry information

Entry nameRPAB4_HUMAN
AccessionPrimary (citable) accession number: P53803
Secondary accession number(s): Q6IBD4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM