P53803 (RPAB4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerases I, II, and III subunit RPABC4
Short name=RNA polymerases I, II, and III subunit ABC4

Alternative name(s):
ABC10-alpha
DNA-directed RNA polymerase II subunit K
RNA polymerase II 7.0 kDa subunit
Short name=RPB7.0
RPB10alpha

Gene names

Name:

POLR2K

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	58 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively.
Subunit structure	Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the archaeal RpoP/eukaryotic RPC10 RNA polymerase subunit family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	7-methylguanosine mRNA capping Traceable author statement. Source: Reactome mRNA splicing, via spliceosome Traceable author statement. Source: Reactome positive regulation of viral transcription Traceable author statement. Source: Reactome regulation of transcription from RNA polymerase I promoter Traceable author statement Ref.1. Source: ProtInc termination of RNA polymerase I transcription Traceable author statement. Source: Reactome termination of RNA polymerase III transcription Traceable author statement. Source: Reactome transcription elongation from RNA polymerase I promoter Traceable author statement. Source: Reactome transcription elongation from RNA polymerase II promoter Traceable author statement. Source: Reactome transcription elongation from RNA polymerase III promoter Traceable author statement. Source: Reactome transcription initiation from RNA polymerase I promoter Traceable author statement. Source: Reactome transcription initiation from RNA polymerase II promoter Traceable author statement. Source: Reactome transcription-coupled nucleotide-excision repair Traceable author statement. Source: Reactome viral reproduction Traceable author statement. Source: Reactome
Cellular_component	nucleoplasm Traceable author statement. Source: Reactome
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 58	58	DNA-directed RNA polymerases I, II, and III subunit RPABC4		PRO_0000159750
Regions
	Zinc finger	19 – 39	21	C4-type Potential
Sites
	Metal binding	19	1	Zinc By similarity
	Metal binding	22	1	Zinc By similarity
	Metal binding	36	1	Zinc By similarity
	Metal binding	39	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	P53803 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 239BA3A67416F02C	FASTA	58	7,004
	10 20 30 40 50 MDTQKDVQPP KQQPMIYICG ECHTENEIKS RDPIRCRECG YRIMYKKRTK RLVVFDAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Four subunits that are shared by the three classes of RNA polymerase are functionally interchangeable between Homo sapiens and Saccharomyces cerevisiae." Shpakovski G.V., Acker J., Wintzerith M., Lacroix J.F., Thuriaux P., Vigneron M. Mol. Cell. Biol. 15:4702-4710(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Organization of genes encoding subunits of eucaryotic nuclear RNA polymerases shows non random intron distribution and correlates with the subunit modular structure." Shpakovski G.V., Lebedenko E.N., Grandemange S., Schaller S., Vigneron M., Kedinger C. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary and Placenta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z47727 mRNA. Translation: CAA87656.1. AJ252078 Genomic DNA. Translation: CAB91873.1. CR456870 mRNA. Translation: CAG33151.1. CH471060 Genomic DNA. Translation: EAW91798.1. BC000806 mRNA. Translation: AAH00806.1. BC018157 mRNA. Translation: AAH18157.1.
IPI	IPI00023975.
PIR	I37558.
RefSeq	NP_005025.1. NM_005034.3.
UniGene	Hs.351475.
3D structure databases
ProteinModelPortal	P53803.
ModBase	Search...
Protein-protein interaction databases
IntAct	P53803. 2 interactions.
STRING	9606.ENSP00000342889.
Polymorphism databases
DMDM	1710664.
Proteomic databases
PRIDE	P53803.
Protocols and materials databases
DNASU	5440.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000353107; ENSP00000342889; ENSG00000147669.
GeneID	5440.
KEGG	hsa:5440.
UCSC	uc003yjf.3. human.
Organism-specific databases
CTD	5440.
GeneCards	GC08P101162.
HGNC	HGNC:9198. POLR2K.
MIM	606033. gene.
neXtProt	NX_P53803.
PharmGKB	PA33518.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1996.
HOVERGEN	HBG054239.
InParanoid	P53803.
KO	K03009.
OMA	VIICREC.
OrthoDB	EOG4M0F3F.
PhylomeDB	P53803.
Enzyme and pathway databases
Reactome	REACT_116125. Disease. REACT_1675. mRNA Processing. REACT_1788. Transcription. REACT_1892. Elongation arrest and recovery. REACT_216. DNA Repair. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	P53803.
Bgee	P53803.
CleanEx	HS_POLR2K.
Genevestigator	P53803.
GermOnline	ENSG00000147669. Homo sapiens.
Family and domain databases
InterPro	IPR006591. RNAP_P/RPC10. [Graphical view]
Pfam	PF03604. DNA_RNApol_7kD. 1 hit. [Graphical view]
SMART	SM00659. RPOLCX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	5440.
NextBio	21051.
SOURCE	Search...

Entry information

Entry name

RPAB4_HUMAN

Accession

Primary (citable) accession number: P53803
Secondary accession number(s): Q6IBD4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.