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Protein

DNA-directed RNA polymerases I, II, and III subunit RPABC4

Gene

POLR2K

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi19 – 191ZincBy similarity
Metal bindingi22 – 221ZincBy similarity
Metal bindingi36 – 361ZincBy similarity
Metal bindingi39 – 391ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri19 – 3921C4-typeSequence analysisAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: InterPro
  • DNA-directed RNA polymerase activity Source: UniProtKB-KW
  • zinc ion binding Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-112382. Formation of RNA Pol II elongation complex.
R-HSA-113418. Formation of the Early Elongation Complex.
R-HSA-167152. Formation of HIV elongation complex in the absence of HIV Tat.
R-HSA-167158. Formation of the HIV-1 Early Elongation Complex.
R-HSA-167160. RNA Pol II CTD phosphorylation and interaction with CE.
R-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-167200. Formation of HIV-1 elongation complex containing HIV-1 Tat.
R-HSA-167238. Pausing and recovery of Tat-mediated HIV elongation.
R-HSA-167242. Abortive elongation of HIV-1 transcript in the absence of Tat.
R-HSA-167243. Tat-mediated HIV elongation arrest and recovery.
R-HSA-167246. Tat-mediated elongation of the HIV-1 transcript.
R-HSA-167287. HIV elongation arrest and recovery.
R-HSA-167290. Pausing and recovery of HIV elongation.
R-HSA-168325. Viral Messenger RNA Synthesis.
R-HSA-1834949. Cytosolic sensors of pathogen-associated DNA.
R-HSA-203927. MicroRNA (miRNA) biogenesis.
R-HSA-427413. NoRC negatively regulates rRNA expression.
R-HSA-452723. Transcriptional regulation of pluripotent stem cells.
R-HSA-5250924. B-WICH complex positively regulates rRNA expression.
R-HSA-5578749. Transcriptional regulation by small RNAs.
R-HSA-5601884. PIWI-interacting RNA (piRNA) biogenesis.
R-HSA-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6781823. Formation of TC-NER Pre-Incision Complex.
R-HSA-6781827. Transcription-Coupled Nucleotide Excision Repair (TC-NER).
R-HSA-6782135. Dual incision in TC-NER.
R-HSA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-HSA-72086. mRNA Capping.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72165. mRNA Splicing - Minor Pathway.
R-HSA-72203. Processing of Capped Intron-Containing Pre-mRNA.
R-HSA-73762. RNA Polymerase I Transcription Initiation.
R-HSA-73772. RNA Polymerase I Promoter Escape.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73777. RNA Polymerase I Chain Elongation.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-73780. RNA Polymerase III Chain Elongation.
R-HSA-73863. RNA Polymerase I Transcription Termination.
R-HSA-73980. RNA Polymerase III Transcription Termination.
R-HSA-749476. RNA Polymerase III Abortive And Retractive Initiation.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-75955. RNA Polymerase II Transcription Elongation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-HSA-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-HSA-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-HSA-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
R-HSA-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerases I, II, and III subunit RPABC4
Short name:
RNA polymerases I, II, and III subunit ABC4
Alternative name(s):
ABC10-alpha
DNA-directed RNA polymerase II subunit K
RNA polymerase II 7.0 kDa subunit
Short name:
RPB7.0
RPB10alpha
Gene namesi
Name:POLR2K
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:9198. POLR2K.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • DNA-directed RNA polymerase I complex Source: GO_Central
  • DNA-directed RNA polymerase II, core complex Source: UniProtKB
  • DNA-directed RNA polymerase III complex Source: GO_Central
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33518.

Polymorphism and mutation databases

BioMutaiPOLR2K.
DMDMi1710664.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5858DNA-directed RNA polymerases I, II, and III subunit RPABC4PRO_0000159750Add
BLAST

Proteomic databases

EPDiP53803.
MaxQBiP53803.
PaxDbiP53803.
PRIDEiP53803.
TopDownProteomicsiP53803.

Expressioni

Gene expression databases

BgeeiP53803.
CleanExiHS_POLR2K.
ExpressionAtlasiP53803. baseline and differential.
GenevisibleiP53803. HS.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively.By similarity

Protein-protein interaction databases

BioGridi111436. 24 interactions.
IntActiP53803. 4 interactions.
STRINGi9606.ENSP00000342889.

Structurei

3D structure databases

ProteinModelPortaliP53803.
SMRiP53803. Positions 15-58.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri19 – 3921C4-typeSequence analysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3507. Eukaryota.
COG1996. LUCA.
GeneTreeiENSGT00390000008918.
HOVERGENiHBG054239.
InParanoidiP53803.
KOiK03009.
OMAiCGAENQL.
OrthoDBiEOG73540T.
PhylomeDBiP53803.
TreeFamiTF103045.

Family and domain databases

Gene3Di2.20.28.30. 1 hit.
InterProiIPR006591. RNAP_P/RPABC4.
IPR029040. RPABC4/Spt4.
[Graphical view]
PfamiPF03604. DNA_RNApol_7kD. 1 hit.
[Graphical view]
SMARTiSM00659. RPOLCX. 1 hit.
[Graphical view]
SUPFAMiSSF63393. SSF63393. 1 hit.

Sequencei

Sequence statusi: Complete.

P53803-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTQKDVQPP KQQPMIYICG ECHTENEIKS RDPIRCRECG YRIMYKKRTK

RLVVFDAR
Length:58
Mass (Da):7,004
Last modified:October 1, 1996 - v1
Checksum:i239BA3A67416F02C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47727 mRNA. Translation: CAA87656.1.
AJ252078 Genomic DNA. Translation: CAB91873.1.
CR456870 mRNA. Translation: CAG33151.1.
CH471060 Genomic DNA. Translation: EAW91798.1.
BC000806 mRNA. Translation: AAH00806.1.
BC018157 mRNA. Translation: AAH18157.1.
CCDSiCCDS6285.1.
PIRiI37558.
RefSeqiNP_005025.1. NM_005034.3.
UniGeneiHs.351475.

Genome annotation databases

EnsembliENST00000353107; ENSP00000342889; ENSG00000147669.
GeneIDi5440.
KEGGihsa:5440.
UCSCiuc003yjf.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47727 mRNA. Translation: CAA87656.1.
AJ252078 Genomic DNA. Translation: CAB91873.1.
CR456870 mRNA. Translation: CAG33151.1.
CH471060 Genomic DNA. Translation: EAW91798.1.
BC000806 mRNA. Translation: AAH00806.1.
BC018157 mRNA. Translation: AAH18157.1.
CCDSiCCDS6285.1.
PIRiI37558.
RefSeqiNP_005025.1. NM_005034.3.
UniGeneiHs.351475.

3D structure databases

ProteinModelPortaliP53803.
SMRiP53803. Positions 15-58.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111436. 24 interactions.
IntActiP53803. 4 interactions.
STRINGi9606.ENSP00000342889.

Polymorphism and mutation databases

BioMutaiPOLR2K.
DMDMi1710664.

Proteomic databases

EPDiP53803.
MaxQBiP53803.
PaxDbiP53803.
PRIDEiP53803.
TopDownProteomicsiP53803.

Protocols and materials databases

DNASUi5440.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000353107; ENSP00000342889; ENSG00000147669.
GeneIDi5440.
KEGGihsa:5440.
UCSCiuc003yjf.4. human.

Organism-specific databases

CTDi5440.
GeneCardsiPOLR2K.
HGNCiHGNC:9198. POLR2K.
MIMi606033. gene.
neXtProtiNX_P53803.
PharmGKBiPA33518.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3507. Eukaryota.
COG1996. LUCA.
GeneTreeiENSGT00390000008918.
HOVERGENiHBG054239.
InParanoidiP53803.
KOiK03009.
OMAiCGAENQL.
OrthoDBiEOG73540T.
PhylomeDBiP53803.
TreeFamiTF103045.

Enzyme and pathway databases

ReactomeiR-HSA-112382. Formation of RNA Pol II elongation complex.
R-HSA-113418. Formation of the Early Elongation Complex.
R-HSA-167152. Formation of HIV elongation complex in the absence of HIV Tat.
R-HSA-167158. Formation of the HIV-1 Early Elongation Complex.
R-HSA-167160. RNA Pol II CTD phosphorylation and interaction with CE.
R-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-167200. Formation of HIV-1 elongation complex containing HIV-1 Tat.
R-HSA-167238. Pausing and recovery of Tat-mediated HIV elongation.
R-HSA-167242. Abortive elongation of HIV-1 transcript in the absence of Tat.
R-HSA-167243. Tat-mediated HIV elongation arrest and recovery.
R-HSA-167246. Tat-mediated elongation of the HIV-1 transcript.
R-HSA-167287. HIV elongation arrest and recovery.
R-HSA-167290. Pausing and recovery of HIV elongation.
R-HSA-168325. Viral Messenger RNA Synthesis.
R-HSA-1834949. Cytosolic sensors of pathogen-associated DNA.
R-HSA-203927. MicroRNA (miRNA) biogenesis.
R-HSA-427413. NoRC negatively regulates rRNA expression.
R-HSA-452723. Transcriptional regulation of pluripotent stem cells.
R-HSA-5250924. B-WICH complex positively regulates rRNA expression.
R-HSA-5578749. Transcriptional regulation by small RNAs.
R-HSA-5601884. PIWI-interacting RNA (piRNA) biogenesis.
R-HSA-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6781823. Formation of TC-NER Pre-Incision Complex.
R-HSA-6781827. Transcription-Coupled Nucleotide Excision Repair (TC-NER).
R-HSA-6782135. Dual incision in TC-NER.
R-HSA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-HSA-72086. mRNA Capping.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72165. mRNA Splicing - Minor Pathway.
R-HSA-72203. Processing of Capped Intron-Containing Pre-mRNA.
R-HSA-73762. RNA Polymerase I Transcription Initiation.
R-HSA-73772. RNA Polymerase I Promoter Escape.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73777. RNA Polymerase I Chain Elongation.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-73780. RNA Polymerase III Chain Elongation.
R-HSA-73863. RNA Polymerase I Transcription Termination.
R-HSA-73980. RNA Polymerase III Transcription Termination.
R-HSA-749476. RNA Polymerase III Abortive And Retractive Initiation.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-75955. RNA Polymerase II Transcription Elongation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-HSA-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-HSA-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-HSA-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
R-HSA-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

GeneWikiiPOLR2K.
GenomeRNAii5440.
NextBioi21051.
PROiP53803.
SOURCEiSearch...

Gene expression databases

BgeeiP53803.
CleanExiHS_POLR2K.
ExpressionAtlasiP53803. baseline and differential.
GenevisibleiP53803. HS.

Family and domain databases

Gene3Di2.20.28.30. 1 hit.
InterProiIPR006591. RNAP_P/RPABC4.
IPR029040. RPABC4/Spt4.
[Graphical view]
PfamiPF03604. DNA_RNApol_7kD. 1 hit.
[Graphical view]
SMARTiSM00659. RPOLCX. 1 hit.
[Graphical view]
SUPFAMiSSF63393. SSF63393. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Four subunits that are shared by the three classes of RNA polymerase are functionally interchangeable between Homo sapiens and Saccharomyces cerevisiae."
    Shpakovski G.V., Acker J., Wintzerith M., Lacroix J.F., Thuriaux P., Vigneron M.
    Mol. Cell. Biol. 15:4702-4710(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Organization of genes encoding subunits of eucaryotic nuclear RNA polymerases shows non random intron distribution and correlates with the subunit modular structure."
    Shpakovski G.V., Lebedenko E.N., Grandemange S., Schaller S., Vigneron M., Kedinger C.
    Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Placenta.
  3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary and Placenta.

Entry informationi

Entry nameiRPAB4_HUMAN
AccessioniPrimary (citable) accession number: P53803
Secondary accession number(s): Q6IBD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 13, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.