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Protein

Squalene synthase

Gene

SQS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)+.

Cofactori

Pathway:ilanosterol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes lanosterol from farnesyl diphosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Squalene synthase (SQS1)
  2. Squalene epoxidase 2, mitochondrial (SQE2), Squalene epoxidase 3 (SQE3), Squalene epoxidase 1 (SQE1), Squalene epoxidase 6 (SQE6), Squalene epoxidase 4 (SQE4), Squalene epoxidase 5 (SQE5)
  3. no protein annotated in this organism
This subpathway is part of the pathway lanosterol biosynthesis, which is itself part of Terpene metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lanosterol from farnesyl diphosphate, the pathway lanosterol biosynthesis and in Terpene metabolism.

GO - Molecular functioni

GO - Biological processi

  • isoprenoid biosynthetic process Source: UniProtKB-KW
  • sterol biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Transferase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

Magnesium, NADP

Enzyme and pathway databases

BioCyciARA:AT4G34640-MONOMER.
MetaCyc:AT4G34640-MONOMER.
BRENDAi2.5.1.21. 399.
ReactomeiREACT_318780. Cholesterol biosynthesis.
UniPathwayiUPA00767; UER00751.

Names & Taxonomyi

Protein namesi
Recommended name:
Squalene synthase (EC:2.5.1.21)
Short name:
SQS
Short name:
SS
Alternative name(s):
FPP:FPP farnesyltransferase
Farnesyl-diphosphate farnesyltransferase
Gene namesi
Name:SQS1
Ordered Locus Names:At4g34640
ORF Names:T4L20.220
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G34640.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei283 – 30321HelicalSequence AnalysisAdd
BLAST
Transmembranei387 – 40721HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • endoplasmic reticulum membrane Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 410409Squalene synthasePRO_0000067455Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP53799.
PRIDEiP53799.

Expressioni

Tissue specificityi

Expressed in all tissues analyzed (inflorescences, leaves, stems and roots). Highly expressed in roots.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi14898. 3 interactions.
IntActiP53799. 3 interactions.
STRINGi3702.AT4G34640.1.

Structurei

3D structure databases

ProteinModelPortaliP53799.
SMRiP53799. Positions 40-358.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phytoene/squalene synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1562.
HOGENOMiHOG000186940.
InParanoidiP53799.
KOiK00801.
OMAiADLEWCY.
PhylomeDBiP53799.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR002060. Squ/phyt_synthse.
IPR006449. Squal_synth.
IPR019845. Squalene/phytoene_synthase_CS.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR01559. squal_synth. 1 hit.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53799-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSLGTMLRY PDDIYPLLKM KRAIEKAEKQ IPPEPHWGFC YSMLHKVSRS
60 70 80 90 100
FSLVIQQLNT ELRNAVCVFY LVLRALDTVE DDTSIPTDEK VPILIAFHRH
110 120 130 140 150
IYDTDWHYSC GTKEYKILMD QFHHVSAAFL ELEKGYQEAI EEITRRMGAG
160 170 180 190 200
MAKFICQEVE TVDDYDEYCH YVAGLVGLGL SKLFLAAGSE VLTPDWEAIS
210 220 230 240 250
NSMGLFLQKT NIIRDYLEDI NEIPKSRMFW PREIWGKYAD KLEDLKYEEN
260 270 280 290 300
TNKSVQCLNE MVTNALMHIE DCLKYMVSLR DPSIFRFCAI PQIMAIGTLA
310 320 330 340 350
LCYNNEQVFR GVVKLRRGLT AKVIDRTKTM ADVYGAFYDF SCMLKTKVDK
360 370 380 390 400
NDPNASKTLN RLEAVQKLCR DAGVLQNRKS YVNDKGQPNS VFIIMVVILL
410
AIVFAYLRAN
Length:410
Mass (Da):47,142
Last modified:October 1, 1996 - v1
Checksum:i6178DD9381A441ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D29017 mRNA. Translation: BAA06103.1.
X86692 mRNA. Translation: CAA60385.1.
AF004560 Genomic DNA. Translation: AAB62242.1.
U79159 mRNA. Translation: AAD00296.1.
AL023094 Genomic DNA. Translation: CAA18843.1.
AL161585 Genomic DNA. Translation: CAB80181.1.
CP002687 Genomic DNA. Translation: AEE86403.1.
AY099868 mRNA. Translation: AAM20719.1.
BT003419 mRNA. Translation: AAO30082.1.
PIRiS54251.
RefSeqiNP_195190.1. NM_119630.3.
UniGeneiAt.20898.
At.67805.

Genome annotation databases

EnsemblPlantsiAT4G34640.1; AT4G34640.1; AT4G34640.
GeneIDi829616.
KEGGiath:AT4G34640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D29017 mRNA. Translation: BAA06103.1.
X86692 mRNA. Translation: CAA60385.1.
AF004560 Genomic DNA. Translation: AAB62242.1.
U79159 mRNA. Translation: AAD00296.1.
AL023094 Genomic DNA. Translation: CAA18843.1.
AL161585 Genomic DNA. Translation: CAB80181.1.
CP002687 Genomic DNA. Translation: AEE86403.1.
AY099868 mRNA. Translation: AAM20719.1.
BT003419 mRNA. Translation: AAO30082.1.
PIRiS54251.
RefSeqiNP_195190.1. NM_119630.3.
UniGeneiAt.20898.
At.67805.

3D structure databases

ProteinModelPortaliP53799.
SMRiP53799. Positions 40-358.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14898. 3 interactions.
IntActiP53799. 3 interactions.
STRINGi3702.AT4G34640.1.

Proteomic databases

PaxDbiP53799.
PRIDEiP53799.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G34640.1; AT4G34640.1; AT4G34640.
GeneIDi829616.
KEGGiath:AT4G34640.

Organism-specific databases

TAIRiAT4G34640.

Phylogenomic databases

eggNOGiCOG1562.
HOGENOMiHOG000186940.
InParanoidiP53799.
KOiK00801.
OMAiADLEWCY.
PhylomeDBiP53799.

Enzyme and pathway databases

UniPathwayiUPA00767; UER00751.
BioCyciARA:AT4G34640-MONOMER.
MetaCyc:AT4G34640-MONOMER.
BRENDAi2.5.1.21. 399.
ReactomeiREACT_318780. Cholesterol biosynthesis.

Miscellaneous databases

PROiP53799.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR002060. Squ/phyt_synthse.
IPR006449. Squal_synth.
IPR019845. Squalene/phytoene_synthase_CS.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR01559. squal_synth. 1 hit.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression, and characterization of cDNAs encoding Arabidopsis thaliana squalene synthase."
    Nakashima T., Inoue T., Oka A., Nishino T., Osumi T., Hata S.
    Proc. Natl. Acad. Sci. U.S.A. 92:2328-2332(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Cloning and characterization of the Arabidopsis thaliana SQS1 gene encoding squalene synthase -- involvement of the C-terminal region of the enzyme in the channeling of squalene through the sterol pathway."
    Kribii R., Arro M., Del Arco A., Gonzalez V., Balcells L., Delourme D., Ferrer A., Karst F., Boronat A.
    Eur. J. Biochem. 249:61-69(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: cv. Landsberg erecta.
  3. Kribii R., Arro M., del Arco A., Gonzalez V., Balcells L.L., Delourme D., Ferrer A., Karst F., Boronat A.
    Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Isolation and characterization of squalene synthase from Arabidopsis thaliana."
    Connolly E.L., Learned R.M.
    Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  6. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  7. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  8. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT GLY-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFDFT_ARATH
AccessioniPrimary (citable) accession number: P53799
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 22, 2015
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.