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P53799

- FDFT_ARATH

UniProt

P53799 - FDFT_ARATH

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Protein

Squalene synthase

Gene

SQS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)+.

Cofactori

Magnesium.

Pathwayi

GO - Molecular functioni

  1. farnesyl-diphosphate farnesyltransferase activity Source: TAIR
  2. oxidoreductase activity Source: UniProtKB-KW
  3. squalene synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. isoprenoid biosynthetic process Source: UniProtKB-KW
  2. sterol biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Transferase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

Magnesium, NADP

Enzyme and pathway databases

BioCyciARA:AT4G34640-MONOMER.
MetaCyc:AT4G34640-MONOMER.
UniPathwayiUPA00767; UER00751.

Names & Taxonomyi

Protein namesi
Recommended name:
Squalene synthase (EC:2.5.1.21)
Short name:
SQS
Short name:
SS
Alternative name(s):
FPP:FPP farnesyltransferase
Farnesyl-diphosphate farnesyltransferase
Gene namesi
Name:SQS1
Ordered Locus Names:At4g34640
ORF Names:T4L20.220
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G34640.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: TAIR
  2. endoplasmic reticulum membrane Source: TAIR
  3. integral component of membrane Source: UniProtKB-KW
  4. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 410409Squalene synthasePRO_0000067455Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP53799.
PRIDEiP53799.

Expressioni

Tissue specificityi

Expressed in all tissues analyzed (inflorescences, leaves, stems and roots). Highly expressed in roots.1 Publication

Gene expression databases

GenevestigatoriP53799.

Interactioni

Protein-protein interaction databases

BioGridi14898. 3 interactions.
IntActiP53799. 3 interactions.
STRINGi3702.AT4G34640.1-P.

Structurei

3D structure databases

ProteinModelPortaliP53799.
SMRiP53799. Positions 40-358.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei283 – 30321HelicalSequence AnalysisAdd
BLAST
Transmembranei387 – 40721HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the phytoene/squalene synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1562.
HOGENOMiHOG000186940.
InParanoidiP53799.
KOiK00801.
OMAiELEEYCW.
PhylomeDBiP53799.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR002060. Squ/phyt_synthse.
IPR006449. Squal_synth.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR01559. squal_synth. 1 hit.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53799 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSLGTMLRY PDDIYPLLKM KRAIEKAEKQ IPPEPHWGFC YSMLHKVSRS
60 70 80 90 100
FSLVIQQLNT ELRNAVCVFY LVLRALDTVE DDTSIPTDEK VPILIAFHRH
110 120 130 140 150
IYDTDWHYSC GTKEYKILMD QFHHVSAAFL ELEKGYQEAI EEITRRMGAG
160 170 180 190 200
MAKFICQEVE TVDDYDEYCH YVAGLVGLGL SKLFLAAGSE VLTPDWEAIS
210 220 230 240 250
NSMGLFLQKT NIIRDYLEDI NEIPKSRMFW PREIWGKYAD KLEDLKYEEN
260 270 280 290 300
TNKSVQCLNE MVTNALMHIE DCLKYMVSLR DPSIFRFCAI PQIMAIGTLA
310 320 330 340 350
LCYNNEQVFR GVVKLRRGLT AKVIDRTKTM ADVYGAFYDF SCMLKTKVDK
360 370 380 390 400
NDPNASKTLN RLEAVQKLCR DAGVLQNRKS YVNDKGQPNS VFIIMVVILL
410
AIVFAYLRAN
Length:410
Mass (Da):47,142
Last modified:October 1, 1996 - v1
Checksum:i6178DD9381A441ED
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D29017 mRNA. Translation: BAA06103.1.
X86692 mRNA. Translation: CAA60385.1.
AF004560 Genomic DNA. Translation: AAB62242.1.
U79159 mRNA. Translation: AAD00296.1.
AL023094 Genomic DNA. Translation: CAA18843.1.
AL161585 Genomic DNA. Translation: CAB80181.1.
CP002687 Genomic DNA. Translation: AEE86403.1.
AY099868 mRNA. Translation: AAM20719.1.
BT003419 mRNA. Translation: AAO30082.1.
PIRiS54251.
RefSeqiNP_195190.1. NM_119630.3.
UniGeneiAt.20898.
At.67805.

Genome annotation databases

EnsemblPlantsiAT4G34640.1; AT4G34640.1; AT4G34640.
GeneIDi829616.
KEGGiath:AT4G34640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D29017 mRNA. Translation: BAA06103.1 .
X86692 mRNA. Translation: CAA60385.1 .
AF004560 Genomic DNA. Translation: AAB62242.1 .
U79159 mRNA. Translation: AAD00296.1 .
AL023094 Genomic DNA. Translation: CAA18843.1 .
AL161585 Genomic DNA. Translation: CAB80181.1 .
CP002687 Genomic DNA. Translation: AEE86403.1 .
AY099868 mRNA. Translation: AAM20719.1 .
BT003419 mRNA. Translation: AAO30082.1 .
PIRi S54251.
RefSeqi NP_195190.1. NM_119630.3.
UniGenei At.20898.
At.67805.

3D structure databases

ProteinModelPortali P53799.
SMRi P53799. Positions 40-358.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 14898. 3 interactions.
IntActi P53799. 3 interactions.
STRINGi 3702.AT4G34640.1-P.

Proteomic databases

PaxDbi P53799.
PRIDEi P53799.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G34640.1 ; AT4G34640.1 ; AT4G34640 .
GeneIDi 829616.
KEGGi ath:AT4G34640.

Organism-specific databases

TAIRi AT4G34640.

Phylogenomic databases

eggNOGi COG1562.
HOGENOMi HOG000186940.
InParanoidi P53799.
KOi K00801.
OMAi ELEEYCW.
PhylomeDBi P53799.

Enzyme and pathway databases

UniPathwayi UPA00767 ; UER00751 .
BioCyci ARA:AT4G34640-MONOMER.
MetaCyc:AT4G34640-MONOMER.

Miscellaneous databases

PROi P53799.

Gene expression databases

Genevestigatori P53799.

Family and domain databases

Gene3Di 1.10.600.10. 1 hit.
InterProi IPR002060. Squ/phyt_synthse.
IPR006449. Squal_synth.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view ]
Pfami PF00494. SQS_PSY. 1 hit.
[Graphical view ]
SUPFAMi SSF48576. SSF48576. 1 hit.
TIGRFAMsi TIGR01559. squal_synth. 1 hit.
PROSITEi PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression, and characterization of cDNAs encoding Arabidopsis thaliana squalene synthase."
    Nakashima T., Inoue T., Oka A., Nishino T., Osumi T., Hata S.
    Proc. Natl. Acad. Sci. U.S.A. 92:2328-2332(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Cloning and characterization of the Arabidopsis thaliana SQS1 gene encoding squalene synthase -- involvement of the C-terminal region of the enzyme in the channeling of squalene through the sterol pathway."
    Kribii R., Arro M., Del Arco A., Gonzalez V., Balcells L., Delourme D., Ferrer A., Karst F., Boronat A.
    Eur. J. Biochem. 249:61-69(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: cv. Landsberg erecta.
  3. Kribii R., Arro M., del Arco A., Gonzalez V., Balcells L.L., Delourme D., Ferrer A., Karst F., Boronat A.
    Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Isolation and characterization of squalene synthase from Arabidopsis thaliana."
    Connolly E.L., Learned R.M.
    Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  6. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  7. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  8. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT GLY-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFDFT_ARATH
AccessioniPrimary (citable) accession number: P53799
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 1, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3