Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Squalene synthase 1

Gene

SQS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)+.2 Publications

Cofactori

Mg2+2 Publications, Mn2+1 Publication

Pathwayi: lanosterol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes lanosterol from farnesyl diphosphate.1 Publication
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Squalene synthase 1 (SQS1)
  2. Squalene epoxidase 2, mitochondrial (SQE2), Squalene epoxidase 3 (SQE3), Squalene epoxidase 1 (SQE1), Squalene epoxidase 6 (SQE6), Squalene epoxidase 4 (SQE4), Squalene epoxidase 5 (SQE5)
  3. no protein annotated in this organism
This subpathway is part of the pathway lanosterol biosynthesis, which is itself part of Terpene metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lanosterol from farnesyl diphosphate, the pathway lanosterol biosynthesis and in Terpene metabolism.

GO - Molecular functioni

  • farnesyl-diphosphate farnesyltransferase activity Source: TAIR
  • squalene synthase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionMultifunctional enzyme, Transferase
Biological processIsoprene biosynthesis
LigandMagnesium, NADP

Enzyme and pathway databases

BioCyciARA:AT4G34640-MONOMER
MetaCyc:AT4G34640-MONOMER
BRENDAi2.5.1.21 399
ReactomeiR-ATH-191273 Cholesterol biosynthesis
UniPathwayiUPA00767; UER00751

Names & Taxonomyi

Protein namesi
Recommended name:
Squalene synthase 11 Publication (EC:2.5.1.212 Publications)
Short name:
SQS 11 Publication
Short name:
SS 11 Publication
Alternative name(s):
FPP:FPP farnesyltransferase 1Curated
Farnesyl-diphosphate farnesyltransferase 1Curated
Gene namesi
Name:SQS11 Publication
Ordered Locus Names:At4g34640
ORF Names:T4L20.220
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G34640
TAIRilocus:2139514 AT4G34640

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei283 – 303HelicalSequence analysisAdd BLAST21
Transmembranei387 – 407HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi287F → S: Drastic reduction of squalene synthase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000674552 – 410Squalene synthase 1Add BLAST409

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP53799
PRIDEiP53799

PTM databases

iPTMnetiP53799

Expressioni

Tissue specificityi

Expressed in all tissues analyzed (seedlings, cotyledons, inflorescences, siliques, leaves, stems and roots). Highly expressed in roots and pollen.2 Publications

Developmental stagei

First observed in very early stages of seedling development. Particularly expressed in the vascular tissues and the petioles.1 Publication

Gene expression databases

ExpressionAtlasiP53799 baseline and differential
GenevisibleiP53799 AT

Interactioni

Protein-protein interaction databases

BioGridi14898, 9 interactors
IntActiP53799, 3 interactors
STRINGi3702.AT4G34640.1

Structurei

3D structure databases

ProteinModelPortaliP53799
SMRiP53799
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phytoene/squalene synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1459 Eukaryota
COG1562 LUCA
HOGENOMiHOG000186940
InParanoidiP53799
KOiK00801
OMAiGEFWTTM
OrthoDBiEOG09360C8N
PhylomeDBiP53799

Family and domain databases

CDDicd00683 Trans_IPPS_HH, 1 hit
Gene3Di1.10.600.10, 1 hit
InterProiView protein in InterPro
IPR008949 Isoprenoid_synthase_dom_sf
IPR002060 Squ/phyt_synthse
IPR006449 Squal_synth-like
IPR019845 Squalene/phytoene_synthase_CS
IPR033904 Trans_IPPS_HH
PfamiView protein in Pfam
PF00494 SQS_PSY, 1 hit
SUPFAMiSSF48576 SSF48576, 1 hit
TIGRFAMsiTIGR01559 squal_synth, 1 hit
PROSITEiView protein in PROSITE
PS01044 SQUALEN_PHYTOEN_SYN_1, 1 hit
PS01045 SQUALEN_PHYTOEN_SYN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53799-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSLGTMLRY PDDIYPLLKM KRAIEKAEKQ IPPEPHWGFC YSMLHKVSRS
60 70 80 90 100
FSLVIQQLNT ELRNAVCVFY LVLRALDTVE DDTSIPTDEK VPILIAFHRH
110 120 130 140 150
IYDTDWHYSC GTKEYKILMD QFHHVSAAFL ELEKGYQEAI EEITRRMGAG
160 170 180 190 200
MAKFICQEVE TVDDYDEYCH YVAGLVGLGL SKLFLAAGSE VLTPDWEAIS
210 220 230 240 250
NSMGLFLQKT NIIRDYLEDI NEIPKSRMFW PREIWGKYAD KLEDLKYEEN
260 270 280 290 300
TNKSVQCLNE MVTNALMHIE DCLKYMVSLR DPSIFRFCAI PQIMAIGTLA
310 320 330 340 350
LCYNNEQVFR GVVKLRRGLT AKVIDRTKTM ADVYGAFYDF SCMLKTKVDK
360 370 380 390 400
NDPNASKTLN RLEAVQKLCR DAGVLQNRKS YVNDKGQPNS VFIIMVVILL
410
AIVFAYLRAN
Length:410
Mass (Da):47,142
Last modified:October 1, 1996 - v1
Checksum:i6178DD9381A441ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D29017 mRNA Translation: BAA06103.1
X86692 mRNA Translation: CAA60385.1
AF004560 Genomic DNA Translation: AAB62242.1
U79159 mRNA Translation: AAD00296.1
AL023094 Genomic DNA Translation: CAA18843.1
AL161585 Genomic DNA Translation: CAB80181.1
CP002687 Genomic DNA Translation: AEE86403.1
AY099868 mRNA Translation: AAM20719.1
BT003419 mRNA Translation: AAO30082.1
PIRiS54251
RefSeqiNP_195190.1, NM_119630.4
UniGeneiAt.20898
At.67805

Genome annotation databases

EnsemblPlantsiAT4G34640.1; AT4G34640.1; AT4G34640
GeneIDi829616
GrameneiAT4G34640.1; AT4G34640.1; AT4G34640
KEGGiath:AT4G34640

Similar proteinsi

Entry informationi

Entry nameiFDFT1_ARATH
AccessioniPrimary (citable) accession number: P53799
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 25, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health