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Protein

Squalene synthase 1

Gene

SQS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)+.2 Publications

Cofactori

Mg2+2 Publications, Mn2+1 Publication

Pathwayi: lanosterol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes lanosterol from farnesyl diphosphate.1 Publication
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Squalene synthase 1 (SQS1)
  2. Squalene epoxidase 3 (SQE3), Squalene epoxidase 2, mitochondrial (SQE2), Squalene epoxidase 1 (SQE1), Squalene epoxidase 6 (SQE6), Squalene epoxidase 4 (SQE4), Squalene epoxidase 5 (SQE5)
  3. no protein annotated in this organism
This subpathway is part of the pathway lanosterol biosynthesis, which is itself part of Terpene metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lanosterol from farnesyl diphosphate, the pathway lanosterol biosynthesis and in Terpene metabolism.

GO - Molecular functioni

  • farnesyl-diphosphate farnesyltransferase activity Source: TAIR
  • oxidoreductase activity Source: UniProtKB-KW
  • squalene synthase activity Source: UniProtKB

GO - Biological processi

  • isoprenoid biosynthetic process Source: UniProtKB-KW
  • sterol biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Transferase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

Magnesium, NADP

Enzyme and pathway databases

BioCyciARA:AT4G34640-MONOMER.
MetaCyc:AT4G34640-MONOMER.
BRENDAi2.5.1.21. 399.
ReactomeiR-ATH-191273. Cholesterol biosynthesis.
UniPathwayiUPA00767; UER00751.

Names & Taxonomyi

Protein namesi
Recommended name:
Squalene synthase 11 Publication (EC:2.5.1.212 Publications)
Short name:
SQS 11 Publication
Short name:
SS 11 Publication
Alternative name(s):
FPP:FPP farnesyltransferase 1Curated
Farnesyl-diphosphate farnesyltransferase 1Curated
Gene namesi
Name:SQS11 Publication
Ordered Locus Names:At4g34640
ORF Names:T4L20.220
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G34640.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei283 – 30321HelicalSequence analysisAdd
BLAST
Transmembranei387 – 40721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • endoplasmic reticulum membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi287 – 2871F → S: Drastic reduction of squalene synthase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 410409Squalene synthase 1PRO_0000067455Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP53799.
PRIDEiP53799.

Expressioni

Tissue specificityi

Expressed in all tissues analyzed (seedlings, cotyledons, inflorescences, siliques, leaves, stems and roots). Highly expressed in roots and pollen.2 Publications

Developmental stagei

First observed in very early stages of seedling development. Particularly expressed in the vascular tissues and the petioles.1 Publication

Gene expression databases

GenevisibleiP53799. AT.

Interactioni

Protein-protein interaction databases

BioGridi14898. 9 interactions.
IntActiP53799. 3 interactions.
STRINGi3702.AT4G34640.1.

Structurei

3D structure databases

ProteinModelPortaliP53799.
SMRiP53799. Positions 40-358.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phytoene/squalene synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1459. Eukaryota.
COG1562. LUCA.
HOGENOMiHOG000186940.
InParanoidiP53799.
KOiK00801.
OMAiADLEWCY.
OrthoDBiEOG09360C8N.
PhylomeDBiP53799.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR002060. Squ/phyt_synthse.
IPR006449. Squal_synth.
IPR019845. Squalene/phytoene_synthase_CS.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR01559. squal_synth. 1 hit.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53799-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSLGTMLRY PDDIYPLLKM KRAIEKAEKQ IPPEPHWGFC YSMLHKVSRS
60 70 80 90 100
FSLVIQQLNT ELRNAVCVFY LVLRALDTVE DDTSIPTDEK VPILIAFHRH
110 120 130 140 150
IYDTDWHYSC GTKEYKILMD QFHHVSAAFL ELEKGYQEAI EEITRRMGAG
160 170 180 190 200
MAKFICQEVE TVDDYDEYCH YVAGLVGLGL SKLFLAAGSE VLTPDWEAIS
210 220 230 240 250
NSMGLFLQKT NIIRDYLEDI NEIPKSRMFW PREIWGKYAD KLEDLKYEEN
260 270 280 290 300
TNKSVQCLNE MVTNALMHIE DCLKYMVSLR DPSIFRFCAI PQIMAIGTLA
310 320 330 340 350
LCYNNEQVFR GVVKLRRGLT AKVIDRTKTM ADVYGAFYDF SCMLKTKVDK
360 370 380 390 400
NDPNASKTLN RLEAVQKLCR DAGVLQNRKS YVNDKGQPNS VFIIMVVILL
410
AIVFAYLRAN
Length:410
Mass (Da):47,142
Last modified:October 1, 1996 - v1
Checksum:i6178DD9381A441ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D29017 mRNA. Translation: BAA06103.1.
X86692 mRNA. Translation: CAA60385.1.
AF004560 Genomic DNA. Translation: AAB62242.1.
U79159 mRNA. Translation: AAD00296.1.
AL023094 Genomic DNA. Translation: CAA18843.1.
AL161585 Genomic DNA. Translation: CAB80181.1.
CP002687 Genomic DNA. Translation: AEE86403.1.
AY099868 mRNA. Translation: AAM20719.1.
BT003419 mRNA. Translation: AAO30082.1.
PIRiS54251.
RefSeqiNP_195190.1. NM_119630.3.
UniGeneiAt.20898.
At.67805.

Genome annotation databases

EnsemblPlantsiAT4G34640.1; AT4G34640.1; AT4G34640.
GeneIDi829616.
GrameneiAT4G34640.1; AT4G34640.1; AT4G34640.
KEGGiath:AT4G34640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D29017 mRNA. Translation: BAA06103.1.
X86692 mRNA. Translation: CAA60385.1.
AF004560 Genomic DNA. Translation: AAB62242.1.
U79159 mRNA. Translation: AAD00296.1.
AL023094 Genomic DNA. Translation: CAA18843.1.
AL161585 Genomic DNA. Translation: CAB80181.1.
CP002687 Genomic DNA. Translation: AEE86403.1.
AY099868 mRNA. Translation: AAM20719.1.
BT003419 mRNA. Translation: AAO30082.1.
PIRiS54251.
RefSeqiNP_195190.1. NM_119630.3.
UniGeneiAt.20898.
At.67805.

3D structure databases

ProteinModelPortaliP53799.
SMRiP53799. Positions 40-358.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14898. 9 interactions.
IntActiP53799. 3 interactions.
STRINGi3702.AT4G34640.1.

Proteomic databases

PaxDbiP53799.
PRIDEiP53799.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G34640.1; AT4G34640.1; AT4G34640.
GeneIDi829616.
GrameneiAT4G34640.1; AT4G34640.1; AT4G34640.
KEGGiath:AT4G34640.

Organism-specific databases

TAIRiAT4G34640.

Phylogenomic databases

eggNOGiKOG1459. Eukaryota.
COG1562. LUCA.
HOGENOMiHOG000186940.
InParanoidiP53799.
KOiK00801.
OMAiADLEWCY.
OrthoDBiEOG09360C8N.
PhylomeDBiP53799.

Enzyme and pathway databases

UniPathwayiUPA00767; UER00751.
BioCyciARA:AT4G34640-MONOMER.
MetaCyc:AT4G34640-MONOMER.
BRENDAi2.5.1.21. 399.
ReactomeiR-ATH-191273. Cholesterol biosynthesis.

Miscellaneous databases

PROiP53799.

Gene expression databases

GenevisibleiP53799. AT.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR002060. Squ/phyt_synthse.
IPR006449. Squal_synth.
IPR019845. Squalene/phytoene_synthase_CS.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR01559. squal_synth. 1 hit.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFDFT1_ARATH
AccessioniPrimary (citable) accession number: P53799
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.