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P53798

- FDFT_MOUSE

UniProt

P53798 - FDFT_MOUSE

Protein

Squalene synthase

Gene

Fdft1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Critical branch point enzyme of isoprenoid biosynthesis that is thought to regulate the flux of isoprene intermediates through the sterol pathway.

    Catalytic activityi

    2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)+.

    Cofactori

    Magnesium.

    Pathwayi

    GO - Molecular functioni

    1. farnesyl-diphosphate farnesyltransferase activity Source: UniProtKB-EC
    2. oxidoreductase activity Source: UniProtKB-KW
    3. squalene synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cholesterol biosynthetic process Source: UniProtKB-KW
    2. farnesyl diphosphate metabolic process Source: Ensembl
    3. isoprenoid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase, Transferase

    Keywords - Biological processi

    Cholesterol biosynthesis, Cholesterol metabolism, Isoprene biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Keywords - Ligandi

    Magnesium, NADP

    Enzyme and pathway databases

    ReactomeiREACT_198602. PPARA activates gene expression.
    REACT_198969. Activation of gene expression by SREBF (SREBP).
    REACT_208531. Cholesterol biosynthesis.
    UniPathwayiUPA00767; UER00751.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Squalene synthase (EC:2.5.1.21)
    Short name:
    SQS
    Short name:
    SS
    Alternative name(s):
    FPP:FPP farnesyltransferase
    Farnesyl-diphosphate farnesyltransferase
    Gene namesi
    Name:Fdft1
    Synonyms:Erg9
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:102706. Fdft1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 416416Squalene synthasePRO_0000067444Add
    BLAST

    Proteomic databases

    MaxQBiP53798.
    PaxDbiP53798.
    PRIDEiP53798.

    PTM databases

    PhosphoSiteiP53798.

    Expressioni

    Gene expression databases

    BgeeiP53798.
    CleanExiMM_FDFT1.
    GenevestigatoriP53798.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000055313.

    Structurei

    3D structure databases

    ProteinModelPortaliP53798.
    SMRiP53798. Positions 35-369.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei284 – 30421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei384 – 40421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the phytoene/squalene synthase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1562.
    GeneTreeiENSGT00390000016034.
    HOGENOMiHOG000186940.
    HOVERGENiHBG002370.
    InParanoidiQ8BPF5.
    KOiK00801.
    OMAiEMRHAVC.
    OrthoDBiEOG7QRQTS.
    TreeFamiTF105316.

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    InterProiIPR002060. Squ/phyt_synthse.
    IPR006449. Squal_synth.
    IPR019845. Squalene/phytoene_synthase_CS.
    IPR008949. Terpenoid_synth.
    [Graphical view]
    PfamiPF00494. SQS_PSY. 1 hit.
    [Graphical view]
    SUPFAMiSSF48576. SSF48576. 1 hit.
    TIGRFAMsiTIGR01559. squal_synth. 1 hit.
    PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
    PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53798-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEFVKCLGHP EEFYNLLRFR MGGRRNFIPK MDQDSLSSSL KTCYKYLNQT    50
    SRSFAAVIQA LDGDIRHAIC VFYLVLRALD TVEDDMSISV EKKIPLLCNF 100
    HTFLYDPEWR FTESKEKDRQ VLEDFPTISL EFRNLAEKYQ TVIDDICHRM 150
    GCGMAEFVDK DVTSKQDWDK YCHYVAGLVG IGLSRLFSAS EFEDPIVGED 200
    IECANSMGLF LQKTNIIRDY LEDQQEGRKF WPQEVWGRYI KKLEDFAKPE 250
    NVDVAVQCLN ELITNTLQHI PDVLTYLSRL RNQSVFNFCA IPQVMAIATL 300
    AACYNNQQVF KGVVKIRKGQ AVTLMMDATN MPAVKAIIYQ YIEEIYHRIP 350
    NSDPSSSKTK QVISKIRTQN LPNCQLISRS HYSPIYLSFI MLLAALSWQY 400
    LSTLSQVTED YVQREH 416
    Length:416
    Mass (Da):48,154
    Last modified:July 27, 2011 - v2
    Checksum:i12C63625DD4FF92B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti149 – 1491R → Q in BAA06102. (PubMed:7999794)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D29016 mRNA. Translation: BAA06102.1.
    AK076062 mRNA. Translation: BAC36156.1.
    AK146766 mRNA. Translation: BAE27418.1.
    AK158633 mRNA. Translation: BAE34590.1.
    AK168629 mRNA. Translation: BAE40489.1.
    AK169317 mRNA. Translation: BAE41072.1.
    CH466535 Genomic DNA. Translation: EDL36069.1.
    BC054722 mRNA. Translation: AAH54722.1.
    BC138301 mRNA. Translation: AAI38302.1.
    BC138302 mRNA. Translation: AAI38303.1.
    CCDSiCCDS27198.1.
    PIRiS52075.
    RefSeqiNP_034321.2. NM_010191.2.
    XP_006518609.1. XM_006518546.1.
    UniGeneiMm.474432.

    Genome annotation databases

    EnsembliENSMUST00000054963; ENSMUSP00000055313; ENSMUSG00000021273.
    GeneIDi14137.
    KEGGimmu:14137.
    UCSCiuc007uhi.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D29016 mRNA. Translation: BAA06102.1 .
    AK076062 mRNA. Translation: BAC36156.1 .
    AK146766 mRNA. Translation: BAE27418.1 .
    AK158633 mRNA. Translation: BAE34590.1 .
    AK168629 mRNA. Translation: BAE40489.1 .
    AK169317 mRNA. Translation: BAE41072.1 .
    CH466535 Genomic DNA. Translation: EDL36069.1 .
    BC054722 mRNA. Translation: AAH54722.1 .
    BC138301 mRNA. Translation: AAI38302.1 .
    BC138302 mRNA. Translation: AAI38303.1 .
    CCDSi CCDS27198.1.
    PIRi S52075.
    RefSeqi NP_034321.2. NM_010191.2.
    XP_006518609.1. XM_006518546.1.
    UniGenei Mm.474432.

    3D structure databases

    ProteinModelPortali P53798.
    SMRi P53798. Positions 35-369.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000055313.

    Chemistry

    ChEMBLi CHEMBL4778.

    PTM databases

    PhosphoSitei P53798.

    Proteomic databases

    MaxQBi P53798.
    PaxDbi P53798.
    PRIDEi P53798.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000054963 ; ENSMUSP00000055313 ; ENSMUSG00000021273 .
    GeneIDi 14137.
    KEGGi mmu:14137.
    UCSCi uc007uhi.2. mouse.

    Organism-specific databases

    CTDi 2222.
    MGIi MGI:102706. Fdft1.

    Phylogenomic databases

    eggNOGi COG1562.
    GeneTreei ENSGT00390000016034.
    HOGENOMi HOG000186940.
    HOVERGENi HBG002370.
    InParanoidi Q8BPF5.
    KOi K00801.
    OMAi EMRHAVC.
    OrthoDBi EOG7QRQTS.
    TreeFami TF105316.

    Enzyme and pathway databases

    UniPathwayi UPA00767 ; UER00751 .
    Reactomei REACT_198602. PPARA activates gene expression.
    REACT_198969. Activation of gene expression by SREBF (SREBP).
    REACT_208531. Cholesterol biosynthesis.

    Miscellaneous databases

    NextBioi 285250.
    PROi P53798.
    SOURCEi Search...

    Gene expression databases

    Bgeei P53798.
    CleanExi MM_FDFT1.
    Genevestigatori P53798.

    Family and domain databases

    Gene3Di 1.10.600.10. 1 hit.
    InterProi IPR002060. Squ/phyt_synthse.
    IPR006449. Squal_synth.
    IPR019845. Squalene/phytoene_synthase_CS.
    IPR008949. Terpenoid_synth.
    [Graphical view ]
    Pfami PF00494. SQS_PSY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48576. SSF48576. 1 hit.
    TIGRFAMsi TIGR01559. squal_synth. 1 hit.
    PROSITEi PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
    PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and functional expression of a cDNA for mouse squalene synthase."
      Inoue T., Osumi T., Hata S.
      Biochim. Biophys. Acta 1260:49-54(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6 X CBA.
      Tissue: Liver.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Amnion, Embryo, Heart, Kidney and Visual cortex.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiFDFT_MOUSE
    AccessioniPrimary (citable) accession number: P53798
    Secondary accession number(s): Q8BPF5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3