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Protein

Sodium/glucose cotransporter 1

Gene

Slc5a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Actively transports glucose into cells by Na+ cotransport with a Na+ to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na+/glucose cotransporter arranged in series along kidney proximal tubules.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei43 – 431Implicated in sodium couplingBy similarity
Sitei300 – 3001Implicated in sodium couplingBy similarity
Binding sitei457 – 4571GlucoseBy similarity
Sitei460 – 4601Involved in sugar-binding/transport and inhibitor bindingBy similarity

GO - Molecular functioni

  • glucose:sodium symporter activity Source: RGD

GO - Biological processi

  • glucose transport Source: RGD
  • intestinal absorption Source: RGD
  • intestinal D-glucose absorption Source: RGD
Complete GO annotation...

Keywords - Biological processi

Ion transport, Sodium transport, Sugar transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiR-RNO-189200. Hexose transport.
R-RNO-428808. Na+-dependent glucose transporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/glucose cotransporter 1
Short name:
Na(+)/glucose cotransporter 1
Alternative name(s):
High affinity sodium-glucose cotransporter
Solute carrier family 5 member 1
Gene namesi
Name:Slc5a1
Synonyms:Sglt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi3713. Slc5a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2828ExtracellularSequence analysisAdd
BLAST
Transmembranei29 – 4921HelicalSequence analysisAdd
BLAST
Topological domaini50 – 6415CytoplasmicSequence analysisAdd
BLAST
Transmembranei65 – 8521HelicalSequence analysisAdd
BLAST
Topological domaini86 – 10520ExtracellularSequence analysisAdd
BLAST
Transmembranei106 – 12621HelicalSequence analysisAdd
BLAST
Topological domaini127 – 14216CytoplasmicSequence analysisAdd
BLAST
Transmembranei143 – 16321HelicalSequence analysisAdd
BLAST
Topological domaini164 – 17815ExtracellularSequence analysisAdd
BLAST
Transmembranei179 – 20123HelicalSequence analysisAdd
BLAST
Topological domaini202 – 2087CytoplasmicSequence analysis
Transmembranei209 – 22921HelicalSequence analysisAdd
BLAST
Topological domaini230 – 27748ExtracellularSequence analysisAdd
BLAST
Transmembranei278 – 29821HelicalSequence analysisAdd
BLAST
Topological domaini299 – 31315CytoplasmicSequence analysisAdd
BLAST
Transmembranei314 – 33421HelicalSequence analysisAdd
BLAST
Topological domaini335 – 38046ExtracellularSequence analysisAdd
BLAST
Transmembranei381 – 40121HelicalSequence analysisAdd
BLAST
Topological domaini402 – 42322CytoplasmicSequence analysisAdd
BLAST
Transmembranei424 – 44421HelicalSequence analysisAdd
BLAST
Topological domaini445 – 45511ExtracellularSequence analysisAdd
BLAST
Transmembranei456 – 47621HelicalSequence analysisAdd
BLAST
Topological domaini477 – 4848CytoplasmicSequence analysis
Transmembranei485 – 50521HelicalSequence analysisAdd
BLAST
Topological domaini506 – 52621ExtracellularSequence analysisAdd
BLAST
Intramembranei527 – 56337HelicalSequence analysisAdd
BLAST
Topological domaini564 – 64481ExtracellularSequence analysisAdd
BLAST
Transmembranei645 – 66521HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • brush border membrane Source: RGD
  • cell-cell junction Source: RGD
  • extracellular exosome Source: Ensembl
  • integral component of plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL5374.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 665665Sodium/glucose cotransporter 1PRO_0000105370Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi248 – 2481N-linked (GlcNAc...)Sequence analysis
Disulfide bondi255 ↔ 611By similarity
Modified residuei588 – 5881PhosphothreonineBy similarity

Post-translational modificationi

N-glycosylation is not necessary for the cotransporter function.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP53790.
PRIDEiP53790.

PTM databases

iPTMnetiP53790.

Expressioni

Developmental stagei

Appears on embryonic day 18 and gradually increases until birth.

Gene expression databases

GenevisibleiP53790. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024165.

Chemistry

BindingDBiP53790.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IP49. Eukaryota.
COG4146. LUCA.
GeneTreeiENSGT00760000118955.
HOGENOMiHOG000025422.
HOVERGENiHBG052859.
InParanoidiP53790.
KOiK14158.
OMAiKAGCTLC.
OrthoDBiEOG77126J.
PhylomeDBiP53790.

Family and domain databases

InterProiIPR001734. Na/solute_symporter.
IPR018212. Na/solute_symporter_CS.
[Graphical view]
PANTHERiPTHR11819. PTHR11819. 2 hits.
PfamiPF00474. SSF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00813. sss. 1 hit.
PROSITEiPS00456. NA_SOLUT_SYMP_1. 1 hit.
PS00457. NA_SOLUT_SYMP_2. 1 hit.
PS50283. NA_SOLUT_SYMP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSSTLSPAV TATDAPIQSY ERIRNAADIS VIVIYFVVVM AVGLWAMFST
60 70 80 90 100
NRGTVGGFFL AGRSMVWWPI GASLFASNIG SGHFVGLAGT GAAAGIAMGG
110 120 130 140 150
FEWNALVFVV VLGWLFVPIY IKAGVVTMPE YLRKRFGGKR IQIYLSVLSL
160 170 180 190 200
LLYIFTKISA DIFSGAIFIN LALGLDIYLA IFILLAITAL YTITGGLAAV
210 220 230 240 250
IYTDTLQTAI MLVGSFILTG FAFREVGGYE AFMDKYMKAI PTLVSDGNIT
260 270 280 290 300
VKEECYTPRA DSFHIFRDPI TGDMPWPGLI FGLSILALWY WCTDQVIVQR
310 320 330 340 350
CLSAKNMSHV KAGCTLCGYL KLLPMFLMVM PGMISRILYT DKIACVLPSE
360 370 380 390 400
CKKYCGTPVG CTNIAYPTLV VELMPNGLRG LMLSVMMASL MSSLTSIFNS
410 420 430 440 450
ASTLFTMDIY TKIRKGASEK ELMIAGRLFI LVLIGISIAW VPIVQSAQSG
460 470 480 490 500
QLFDYIQSIT SYLGPPIAAV FLLAIFCKRV NEPGAFWGLI LGFLIGISRM
510 520 530 540 550
ITEFAYGTGS CMEPSNCPKI ICGVHYLYFA IILFAISVVT VLVISLLTKP
560 570 580 590 600
IPDVHLYRLC WSLRNSTEER IDLDAGEEEP VEEDPKDTIE IDAEAPQKEK
610 620 630 640 650
GCFRKAYDLF CGLDQDKGPK MTKEEEEAMK LKMTDTSEKP LWRTVVNING
660
IILLAVAVFC HAYFA
Length:665
Mass (Da):73,066
Last modified:October 1, 1996 - v1
Checksum:iA92038D964BFF061
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti354 – 3541Y → H in BAA19172 (PubMed:9214758).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03120 mRNA. Translation: AAA19015.1.
D16101 mRNA. Translation: BAA03676.1.
AB000729 mRNA. Translation: BAA19172.1.
BC081827 mRNA. Translation: AAH81827.1.
PIRiA53582.
RefSeqiNP_037165.1. NM_013033.2.
UniGeneiRn.10224.

Genome annotation databases

EnsembliENSRNOT00000024165; ENSRNOP00000024165; ENSRNOG00000017775.
GeneIDi25552.
KEGGirno:25552.
UCSCiRGD:3713. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03120 mRNA. Translation: AAA19015.1.
D16101 mRNA. Translation: BAA03676.1.
AB000729 mRNA. Translation: BAA19172.1.
BC081827 mRNA. Translation: AAH81827.1.
PIRiA53582.
RefSeqiNP_037165.1. NM_013033.2.
UniGeneiRn.10224.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024165.

Chemistry

BindingDBiP53790.
ChEMBLiCHEMBL5374.

PTM databases

iPTMnetiP53790.

Proteomic databases

PaxDbiP53790.
PRIDEiP53790.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024165; ENSRNOP00000024165; ENSRNOG00000017775.
GeneIDi25552.
KEGGirno:25552.
UCSCiRGD:3713. rat.

Organism-specific databases

CTDi6523.
RGDi3713. Slc5a1.

Phylogenomic databases

eggNOGiENOG410IP49. Eukaryota.
COG4146. LUCA.
GeneTreeiENSGT00760000118955.
HOGENOMiHOG000025422.
HOVERGENiHBG052859.
InParanoidiP53790.
KOiK14158.
OMAiKAGCTLC.
OrthoDBiEOG77126J.
PhylomeDBiP53790.

Enzyme and pathway databases

ReactomeiR-RNO-189200. Hexose transport.
R-RNO-428808. Na+-dependent glucose transporters.

Miscellaneous databases

PROiP53790.

Gene expression databases

GenevisibleiP53790. RN.

Family and domain databases

InterProiIPR001734. Na/solute_symporter.
IPR018212. Na/solute_symporter_CS.
[Graphical view]
PANTHERiPTHR11819. PTHR11819. 2 hits.
PfamiPF00474. SSF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00813. sss. 1 hit.
PROSITEiPS00456. NA_SOLUT_SYMP_1. 1 hit.
PS00457. NA_SOLUT_SYMP_2. 1 hit.
PS50283. NA_SOLUT_SYMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The high affinity Na+/glucose cotransporter. Re-evaluation of function and distribution of expression."
    Lee W.S., Kanai Y., Wells R.G., Hediger M.A.
    J. Biol. Chem. 269:12032-12039(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  2. Kasahara M., Mori K.
    Submitted (MAY-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The sugar specificity of Na+/glucose cotransporter from rat jejunum."
    Aoshima H., Yokoyama T., Tanizaki J., Izu H., Yamada M.
    Biosci. Biotechnol. Biochem. 61:979-983(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Jejunum.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiSC5A1_RAT
AccessioniPrimary (citable) accession number: P53790
Secondary accession number(s): P97787
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.