Reviewed, UniProtKB/Swiss-Prot P53786 (ZP3_CALSQ)

Names and origin

Protein names

Recommended name:
    Zona pellucida sperm-binding protein 3
Alternative name(s):
    Zona pellucida glycoprotein ZP3
    Zona pellucida protein C
    Sperm receptor
Cleaved into the following chain:
    1- Recommended name:
            Processed zona pellucida sperm-binding protein 3

Gene names

Name:	ZP3
Synonyms:	ZPC

Organism

Callithrix sp. (Marmoset)

Taxonomic identifier

9485 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Platyrrhini › Cebidae › Callitrichinae › Callithrix

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.
Subunit structure	Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers By similarity.
Subcellular location	Processed zona pellucida sperm-binding protein 3: Secreted › extracellular space › extracellular matrix. Cell membrane; Single-pass type I membrane protein.
Tissue specificity	Oocytes.
Domain	The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.
Post-translational modification	Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida. N-glycosylated By similarity. O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy By similarity.
Sequence similarities	Belongs to the ZP domain family. ZPC subfamily. Contains 1 ZP domain.

Ontologies

Keywords
Biological process	Fertilization
Cellular component	Cell membrane Extracellular matrix Membrane Secreted
Domain	Signal Transmembrane
Molecular function	Receptor
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Pyrrolidone carboxylic acid
Gene Ontology (GO)
Biological process	single fertilization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	By similarity
	Chain	23 – 350	328	Zona pellucida sperm-binding protein 3		PRO_0000041703
	Chain	23 – ?		Processed zona pellucida sperm-binding protein 3		PRO_0000304566
	Propeptide	351 – 424	74	Removed in mature form By similarity		PRO_0000041704
Regions
	Topological domain	23 – 387	365	Extracellular Potential
	Transmembrane	388 – 408	21	Potential
	Topological domain	409 – 424	16	Cytoplasmic Potential
	Domain	45 – 307	263	ZP
Amino acid modifications
	Modified residue	23	1	Pyrrolidone carboxylic acid By similarity
	Glycosylation	32	1	O-linked (GalNAc...) By similarity
	Glycosylation	125	1	N-linked (GlcNAc...) By similarity
	Glycosylation	147	1	N-linked (GlcNAc...) By similarity
	Glycosylation	156	1	O-linked (GalNAc...) By similarity
	Glycosylation	162	1	O-linked (GalNAc...) By similarity
	Glycosylation	163	1	O-linked (GalNAc...) By similarity
	Glycosylation	180	1	N-linked (GlcNAc...) Potential
	Glycosylation	272	1	N-linked (GlcNAc...) By similarity
	Disulfide bond	46 ↔ 140		By similarity
	Disulfide bond	78 ↔ 99		By similarity
	Disulfide bond	217 ↔ 282		By similarity
	Disulfide bond	239 ↔ 300		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P53786-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 1DACBD03026C2739	FASTA	424	46,809
	10 20 30 40 50 60 MELSYRLFIC LLLWGSTELC YPQPLRLLQG GTSHPETALQ PVVVECQEAT LVVTVSKDLF 70 80 90 100 110 120 GTRKLIRAVD LTLGPEGCEP LVSTDTEDVV RFEVGLHECG NSMQVTDDAL VYSTFLLHDP 130 140 150 160 170 180 RPVGNLSIVR TNRAEIPIEC RYPRRGNVSS QAILPTWLPF RTTVFSEEKL TFSLRLMEEN 190 200 210 220 230 240 WSTEKRTPTF HLGDVAHLQA EIHTGSHVPL RLFVDHCVAT PTPDQNASPY HTIVDFHGCL 250 260 270 280 290 300 VDGLTDASSA FQAPRPRPDT LQFTVDVFHF ANDSRNMIYI TCHLKVTLAE QDPDELNKAC 310 320 330 340 350 360 SFSKASNSWF PVEGPADICQ CCSKGDCGTP SHARRQPHVV SLGSGSPARD RRHVTEEADV 370 380 390 400 410 420 TVGPLIFLDR TGDHEMEQWA LPADTSLLLL GTGLAVVALL TLTAVILVLT RRCRTASLPV SASE

References

[1]	"Cloning, sequencing and oocyte-specific expression of the marmoset sperm receptor protein, ZP3." Thillai-Koothan P., van Duin M., Aitken R.J. Zygote 1:93-101(1993) [PubMed: 8081814] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.

Web resources

Protein Spotlight

Molecular chastity - Issue 93 of April 2008

Cross-references

Sequence databases
EMBLGenBankDDBJ	S71825 mRNA. Translation: AAB31866.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	P53786.
Family and domain databases
InterPro	IPR001507. Endoglin/CD105. IPR017977. Endoglin/CD105_CS. IPR017976. Endoglin/CD105_subgr. [Graphical view]
Pfam	PF00100. Zona_pellucida. 1 hit. [Graphical view]
PRINTS	PR00023. ZPELLUCIDA.
SMART	SM00241. ZP. 1 hit. [Graphical view]
PROSITE	PS00682. ZP_1. 1 hit. PS51034. ZP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ZP3_CALSQ

Accession

Primary (citable) accession number: P53786

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	October 13, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)