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Protein

Zona pellucida sperm-binding protein 3

Gene

ZP3

Organism
Macaca radiata (Bonnet macaque)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Fertilization

Names & Taxonomyi

Protein namesi
Recommended name:
Zona pellucida sperm-binding protein 3
Alternative name(s):
Sperm receptor
Zona pellucida glycoprotein 3
Short name:
Zp-3
Zona pellucida protein C
Cleaved into the following chain:
Gene namesi
Name:ZP3
Synonyms:ZPC
OrganismiMacaca radiata (Bonnet macaque)
Taxonomic identifieri9548 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

Chain Processed zona pellucida sperm-binding protein 3 :

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 387365ExtracellularSequence AnalysisAdd
BLAST
Transmembranei388 – 40821HelicalSequence AnalysisAdd
BLAST
Topological domaini409 – 42416CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Extracellular matrix, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222By similarityAdd
BLAST
Chaini23 – 350328Zona pellucida sperm-binding protein 3PRO_0000041711Add
BLAST
Chaini23 – ?Processed zona pellucida sperm-binding protein 3PRO_0000304570
Propeptidei351 – 42474Removed in mature formBy similarityPRO_0000041712Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei23 – 231Pyrrolidone carboxylic acidBy similarity
Disulfide bondi46 ↔ 140By similarity
Disulfide bondi78 ↔ 99By similarity
Glycosylationi125 – 1251N-linked (GlcNAc...)By similarity
Glycosylationi147 – 1471N-linked (GlcNAc...)By similarity
Glycosylationi156 – 1561O-linked (GalNAc...)By similarity
Glycosylationi162 – 1621O-linked (GalNAc...)By similarity
Glycosylationi163 – 1631O-linked (GalNAc...)By similarity
Disulfide bondi217 ↔ 282By similarity
Disulfide bondi239 ↔ 300By similarity
Glycosylationi272 – 2721N-linked (GlcNAc...)By similarity

Post-translational modificationi

Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
N-glycosylated.By similarity
O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Expressioni

Tissue specificityi

Oocytes.

Interactioni

Subunit structurei

Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers.By similarity

Structurei

3D structure databases

ProteinModelPortaliP53785.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 307263ZPPROSITE-ProRule annotationAdd
BLAST

Domaini

The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.

Sequence similaritiesi

Belongs to the ZP domain family. ZPC subfamily.Curated
Contains 1 ZP domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG007985.

Family and domain databases

InterProiIPR001507. ZP_dom.
IPR017977. ZP_dom_CS.
[Graphical view]
PfamiPF00100. Zona_pellucida. 1 hit.
[Graphical view]
PRINTSiPR00023. ZPELLUCIDA.
SMARTiSM00241. ZP. 1 hit.
[Graphical view]
PROSITEiPS00682. ZP_1. 1 hit.
PS51034. ZP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53785-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELSYRLFIC LLLWGSTELC YPQPFWLLQG GASRAETSVQ PVLVECREAT
60 70 80 90 100
LLVTVSKDLF GTGKLIRAAD LTLGPEACEP LVSMDTEDVV RFEVGLHECG
110 120 130 140 150
SSMQVTDDAL VYSTFLLHDP RPVGNLSIVR TNRAEIPIEC RYPRQGNVSS
160 170 180 190 200
QAILPTWLPF RTTVFSEEKL TFSLRLMEEN WNAEKRSPTF HLGDAAHLQA
210 220 230 240 250
EIHTGSHVPL RLFVDHCVAT PTPDQNASPY HTIVDFHGCL VDGLTDASSA
260 270 280 290 300
FKVPRPGPDT LQFTVDVFHF ANDSRNMIYI TCHLKAIPAE QEPDELNKAC
310 320 330 340 350
SFSKSSNSWF PVEGPADICQ CCSKGDCGTP SHSRRQPHVV SQWSRSASRN
360 370 380 390 400
RRHVTEEADI TVGPLIFLDR SADYEVEQWA LPADTSVLLL GIGLAVVASL
410 420
TLTAVILIFT RRWRTASRPV SASE
Length:424
Mass (Da):47,040
Last modified:October 1, 1996 - v1
Checksum:i3B41C4CFA3792331
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82639 mRNA. Translation: CAA57961.1.

Cross-referencesi

Web resourcesi

Protein Spotlight

Molecular chastity - Issue 93 of April 2008

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82639 mRNA. Translation: CAA57961.1.

3D structure databases

ProteinModelPortaliP53785.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG007985.

Family and domain databases

InterProiIPR001507. ZP_dom.
IPR017977. ZP_dom_CS.
[Graphical view]
PfamiPF00100. Zona_pellucida. 1 hit.
[Graphical view]
PRINTSiPR00023. ZPELLUCIDA.
SMARTiSM00241. ZP. 1 hit.
[Graphical view]
PROSITEiPS00682. ZP_1. 1 hit.
PS51034. ZP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of cDNA encoding bonnet monkey (Macaca radiata) zona pellucida glycoprotein-ZP3."
    Kolluri S.K., Kaul R., Banerjee K., Gupta S.K.
    Reprod. Fertil. Dev. 7:1209-1212(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiZP3_MACRA
AccessioniPrimary (citable) accession number: P53785
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 27, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.