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P53785

- ZP3_MACRA

UniProt

P53785 - ZP3_MACRA

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Protein
Zona pellucida sperm-binding protein 3
Gene
ZP3, ZPC
Organism
Macaca radiata (Bonnet macaque)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.

GO - Molecular functioni

  1. carbohydrate binding Source: UniProtKB
  2. manganese ion transmembrane transporter activity Source: UniProtKB
  3. signal transducer activity Source: UniProtKB

GO - Biological processi

  1. binding of sperm to zona pellucida Source: UniProtKB
  2. blastocyst formation Source: UniProtKB
  3. egg coat formation Source: UniProtKB
  4. humoral immune response mediated by circulating immunoglobulin Source: UniProtKB
  5. intracellular protein transport Source: UniProtKB
  6. intracellular signal transduction Source: UniProtKB
  7. manganese ion transmembrane transport Source: GOC
  8. manganese ion transport Source: UniProtKB
  9. negative regulation of binding of sperm to zona pellucida Source: UniProtKB
  10. negative regulation of transcription, DNA-templated Source: UniProtKB
  11. oocyte development Source: UniProtKB
  12. phosphatidylinositol-mediated signaling Source: UniProtKB
  13. positive regulation of T cell proliferation Source: UniProtKB
  14. positive regulation of acrosomal vesicle exocytosis Source: UniProtKB
  15. positive regulation of acrosome reaction Source: UniProtKB
  16. positive regulation of antral ovarian follicle growth Source: UniProtKB
  17. positive regulation of calcium ion import Source: UniProtKB
  18. positive regulation of humoral immune response Source: UniProtKB
  19. positive regulation of inflammatory response Source: UniProtKB
  20. positive regulation of interferon-gamma production Source: UniProtKB
  21. positive regulation of interleukin-4 production Source: UniProtKB
  22. positive regulation of leukocyte migration Source: UniProtKB
  23. positive regulation of ovarian follicle development Source: UniProtKB
  24. positive regulation of phosphatidylinositol biosynthetic process Source: UniProtKB
  25. positive regulation of protein kinase B signaling Source: UniProtKB
  26. positive regulation of protein kinase activity Source: UniProtKB
  27. positive regulation of transcription, DNA-templated Source: UniProtKB
  28. positive regulation of type IV hypersensitivity Source: UniProtKB
  29. protein kinase C signaling Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Fertilization

Names & Taxonomyi

Protein namesi
Recommended name:
Zona pellucida sperm-binding protein 3
Alternative name(s):
Sperm receptor
Zona pellucida glycoprotein 3
Short name:
Zp-3
Zona pellucida protein C
Cleaved into the following chain:
Gene namesi
Name:ZP3
Synonyms:ZPC
OrganismiMacaca radiata (Bonnet macaque)
Taxonomic identifieri9548 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

Chain Processed zona pellucida sperm-binding protein 3 : Secretedextracellular spaceextracellular matrix
Note: The glycoproteinaceous translucent extracellular matrix that surrounds the mammalian oocyte is called zona pellucida.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 387365Extracellular Reviewed prediction
Add
BLAST
Transmembranei388 – 40821Helical; Reviewed prediction
Add
BLAST
Topological domaini409 – 42416Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB
  2. cytoplasm Source: UniProtKB
  3. endoplasmic reticulum Source: UniProtKB
  4. extracellular matrix Source: UniProtKB
  5. extracellular space Source: UniProtKB
  6. integral component of membrane Source: UniProtKB-KW
  7. multivesicular body Source: UniProtKB
  8. outer acrosomal membrane Source: UniProtKB
  9. perinuclear region of cytoplasm Source: UniProtKB
  10. plasma membrane Source: UniProtKB
  11. proteinaceous extracellular matrix Source: UniProtKB-SubCell
  12. secretory granule Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Extracellular matrix, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 By similarity
Add
BLAST
Chaini23 – 350328Zona pellucida sperm-binding protein 3
PRO_0000041711Add
BLAST
Chaini23 – ?Processed zona pellucida sperm-binding protein 3PRO_0000304570
Propeptidei351 – 42474Removed in mature form By similarity
PRO_0000041712Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei23 – 231Pyrrolidone carboxylic acid By similarity
Disulfide bondi46 ↔ 140 By similarity
Disulfide bondi78 ↔ 99 By similarity
Glycosylationi125 – 1251N-linked (GlcNAc...) By similarity
Glycosylationi147 – 1471N-linked (GlcNAc...) By similarity
Glycosylationi156 – 1561O-linked (GalNAc...) By similarity
Glycosylationi162 – 1621O-linked (GalNAc...) By similarity
Glycosylationi163 – 1631O-linked (GalNAc...) By similarity
Disulfide bondi217 ↔ 282 By similarity
Disulfide bondi239 ↔ 300 By similarity
Glycosylationi272 – 2721N-linked (GlcNAc...) By similarity

Post-translational modificationi

Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
N-glycosylated By similarity.
O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy By similarity.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Expressioni

Tissue specificityi

Oocytes.

Interactioni

Subunit structurei

Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers By similarity.

Structurei

3D structure databases

ProteinModelPortaliP53785.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 307263ZP
Add
BLAST

Domaini

The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.

Sequence similaritiesi

Contains 1 ZP domain.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG007985.

Family and domain databases

InterProiIPR001507. ZP_dom.
IPR017977. ZP_dom_CS.
[Graphical view]
PfamiPF00100. Zona_pellucida. 1 hit.
[Graphical view]
PRINTSiPR00023. ZPELLUCIDA.
SMARTiSM00241. ZP. 1 hit.
[Graphical view]
PROSITEiPS00682. ZP_1. 1 hit.
PS51034. ZP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53785-1 [UniParc]FASTAAdd to Basket

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MELSYRLFIC LLLWGSTELC YPQPFWLLQG GASRAETSVQ PVLVECREAT    50
LLVTVSKDLF GTGKLIRAAD LTLGPEACEP LVSMDTEDVV RFEVGLHECG 100
SSMQVTDDAL VYSTFLLHDP RPVGNLSIVR TNRAEIPIEC RYPRQGNVSS 150
QAILPTWLPF RTTVFSEEKL TFSLRLMEEN WNAEKRSPTF HLGDAAHLQA 200
EIHTGSHVPL RLFVDHCVAT PTPDQNASPY HTIVDFHGCL VDGLTDASSA 250
FKVPRPGPDT LQFTVDVFHF ANDSRNMIYI TCHLKAIPAE QEPDELNKAC 300
SFSKSSNSWF PVEGPADICQ CCSKGDCGTP SHSRRQPHVV SQWSRSASRN 350
RRHVTEEADI TVGPLIFLDR SADYEVEQWA LPADTSVLLL GIGLAVVASL 400
TLTAVILIFT RRWRTASRPV SASE 424
Length:424
Mass (Da):47,040
Last modified:October 1, 1996 - v1
Checksum:i3B41C4CFA3792331
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X82639 mRNA. Translation: CAA57961.1.

Cross-referencesi

Web resourcesi

Protein Spotlight

Molecular chastity - Issue 93 of April 2008

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X82639 mRNA. Translation: CAA57961.1 .

3D structure databases

ProteinModelPortali P53785.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG007985.

Family and domain databases

InterProi IPR001507. ZP_dom.
IPR017977. ZP_dom_CS.
[Graphical view ]
Pfami PF00100. Zona_pellucida. 1 hit.
[Graphical view ]
PRINTSi PR00023. ZPELLUCIDA.
SMARTi SM00241. ZP. 1 hit.
[Graphical view ]
PROSITEi PS00682. ZP_1. 1 hit.
PS51034. ZP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of cDNA encoding bonnet monkey (Macaca radiata) zona pellucida glycoprotein-ZP3."
    Kolluri S.K., Kaul R., Banerjee K., Gupta S.K.
    Reprod. Fertil. Dev. 7:1209-1212(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiZP3_MACRA
AccessioniPrimary (citable) accession number: P53785
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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