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P53784 (SOX3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor SOX-3
Gene names
Name:Sox3
Synonyms:Sox-3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length375 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor required during the formation of the hypothalamo-pituitary axis. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Required also within the pharyngeal epithelia for craniofacial morphogenesis. Controls a genetic switch in male development. Is necessary for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells. Ref.4 Ref.5 Ref.6

Subunit structure

Interacts with SOX2 and FGFR1. Ref.5

Subcellular location

Nucleus.

Tissue specificity

Mainly in the developing central nervous system. Expressed in developing urogenital ridge.

Sequence similarities

Contains 1 HMG box DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
   Molecular functionDevelopmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processSertoli cell development

Inferred from expression pattern PubMed 11875113. Source: UniProtKB

face development

Inferred from mutant phenotype Ref.5. Source: UniProtKB

forebrain development

Inferred from mutant phenotype Ref.4. Source: MGI

hypothalamus development

Inferred from mutant phenotype Ref.4. Source: UniProtKB

negative regulation of neuron differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 11875113. Source: GOC

organ morphogenesis

Inferred from mutant phenotype Ref.4. Source: MGI

pituitary gland development

Inferred from mutant phenotype Ref.4. Source: UniProtKB

sensory organ development

Inferred from mutant phenotype Ref.5. Source: UniProtKB

sex determination

Inferred from mutant phenotype Ref.6. Source: UniProtKB

spermatid differentiation

Inferred from mutant phenotype PubMed 15893302. Source: MGI

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from direct assay Ref.1. Source: MGI

RNA polymerase II core promoter sequence-specific DNA binding

Inferred from direct assay PubMed 11875113. Source: UniProtKB

RNA polymerase II transcription corepressor activity

Inferred from direct assay PubMed 11875113. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 375375Transcription factor SOX-3
PRO_0000048721

Regions

DNA binding69 – 13769HMG box
Compositional bias54 – 6310Poly-Gly
Compositional bias164 – 17714Poly-Ala
Compositional bias219 – 2235Poly-Pro
Compositional bias252 – 2598Poly-Ala
Compositional bias269 – 2768Poly-Ala
Compositional bias282 – 29312Poly-Ala

Experimental info

Sequence conflict1601L → P in CAA63845. Ref.1
Sequence conflict1601L → P in AAL40744. Ref.2
Sequence conflict3151A → R in CAA63845. Ref.1
Sequence conflict3151A → R in AAL40744. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P53784 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 3E117E20D40BFE34

FASTA37537,789
        10         20         30         40         50         60 
MYSLLETELK NPVGPPTPAA GTGVPAAPGA AGKSGANPAG GANAGNGGSG GANGGGGGGG 

        70         80         90        100        110        120 
GGGSDQDRVK RPMNAFMVWS RGQRRKMALE NPKMHNSEIS KRLGADWKLL TDAEKRPFID 

       130        140        150        160        170        180 
EAKRLRAVHM KEYPDYKYRP RRKTKTLLKK DKYSLPGGLL PPGAAAAAAA AAAAAAASSP 

       190        200        210        220        230        240 
VGVGQRLDTY THVNGWANGA YSLVQEQLGY AQPPSMSSPP PPPALPQMHR YDMAGLQYSP 

       250        260        270        280        290        300 
MMPPGAQSYM NAAAAAAAAS GYGGMAPSAA AAAAAAYGQQ PATAAAAAAA AAAMSLGPMG 

       310        320        330        340        350        360 
SVVKSEPSSP PPAIASHSQR ACLGDLRDMI SMYLPPGGDA ADAASPLPGG RLHGVHQHYQ 

       370 
GAGTAVNGTV PLTHI 

« Hide

References

[1]"A comparison of the properties of Sox-3 with Sry and two related genes, Sox-1 and Sox-2."
Collignon J., Sockanathan S., Hacker A., Cohen-Tannoudji M., Norris D., Rastan S., Stevanovic M., Goodfellow P.N., Lovell-Badge R.
Development 122:509-520(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129.
[2]"Expression of Sox3 throughout the developing central nervous system is dependent on the combined action of discrete, evolutionarily conserved regulatory elements."
Brunelli S., Silva Casey E., Bell D., Harland R., Lovell-Badge R.
Genesis 36:12-24(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
[3]"Interplay of SOX and POU factors in regulation of the nestin gene in neural primordial cells."
Tanaka S., Kamachi Y., Tanouchi A., Hamada H., Jing N., Kondoh H.
Mol. Cell. Biol. 24:8834-8846(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: 129/Ola.
[4]"SOX3 is required during the formation of the hypothalamo-pituitary axis."
Rizzoti K., Brunelli S., Carmignac D., Thomas P.Q., Robinson I.C., Lovell-Badge R.
Nat. Genet. 36:247-255(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"SOX3 activity during pharyngeal segmentation is required for craniofacial morphogenesis."
Rizzoti K., Lovell-Badge R.
Development 134:3437-3448(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SOX2 AND FGFR1.
[6]"Identification of SOX3 as an XX male sex reversal gene in mice and humans."
Sutton E., Hughes J., White S., Sekido R., Tan J., Arboleda V., Rogers N., Knower K., Rowley L., Eyre H., Rizzoti K., McAninch D., Goncalves J., Slee J., Turbitt E., Bruno D., Bengtsson H., Harley V. expand/collapse author list , Vilain E., Sinclair A., Lovell-Badge R., Thomas P.
J. Clin. Invest. 121:328-341(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X94125 Genomic DNA. Translation: CAA63845.1.
AF434675 Genomic DNA. Translation: AAL40744.1.
AB108674 mRNA. Translation: BAC75669.1.
PIRS10948.
UniGeneMm.35784.

3D structure databases

ProteinModelPortalP53784.
SMRP53784. Positions 67-145.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP53784.

Proteomic databases

PaxDbP53784.
PRIDEP53784.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:98365. Sox3.

Phylogenomic databases

eggNOGNOG321816.
HOGENOMHOG000231647.
HOVERGENHBG105663.
InParanoidQ80XF1.

Gene expression databases

BgeeP53784.
CleanExMM_SOX3.
GenevestigatorP53784.

Family and domain databases

Gene3D1.10.30.10. 1 hit.
InterProIPR009071. HMG_box_dom.
IPR022097. TF_SOX.
[Graphical view]
PfamPF00505. HMG_box. 1 hit.
PF12336. SOXp. 1 hit.
[Graphical view]
SMARTSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMSSF47095. SSF47095. 1 hit.
PROSITEPS50118. HMG_BOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSOX3. mouse.
PROP53784.
SOURCESearch...

Entry information

Entry nameSOX3_MOUSE
AccessionPrimary (citable) accession number: P53784
Secondary accession number(s): Q80XF1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot