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P53762

- ARNT_MOUSE

UniProt

P53762 - ARNT_MOUSE

Protein

Aryl hydrocarbon receptor nuclear translocator

Gene

Arnt

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia By similarity.By similarity

    GO - Molecular functioni

    1. aryl hydrocarbon receptor activity Source: MGI
    2. DNA binding Source: MGI
    3. enhancer binding Source: MGI
    4. protein binding Source: IntAct
    5. protein heterodimerization activity Source: MGI
    6. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: Ensembl
    7. sequence-specific DNA binding Source: Ensembl
    8. sequence-specific DNA binding transcription factor activity Source: MGI
    9. transcription coactivator activity Source: MGI
    10. transcription factor binding transcription factor activity Source: MGI

    GO - Biological processi

    1. cell differentiation Source: MGI
    2. embryonic placenta development Source: MGI
    3. intracellular receptor signaling pathway Source: GOC
    4. positive regulation of hormone biosynthetic process Source: Ensembl
    5. positive regulation of protein sumoylation Source: BHF-UCL
    6. positive regulation of transcription, DNA-templated Source: MGI
    7. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    8. positive regulation vascular endothelial growth factor production Source: Ensembl
    9. regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: Ensembl
    10. response to hypoxia Source: MGI
    11. response to toxic substance Source: MGI

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_199107. Regulation of gene expression by Hypoxia-inducible Factor.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aryl hydrocarbon receptor nuclear translocator
    Short name:
    ARNT protein
    Alternative name(s):
    Dioxin receptor, nuclear translocator
    Hypoxia-inducible factor 1-beta
    Short name:
    HIF-1-beta
    Short name:
    HIF1-beta
    Gene namesi
    Name:Arnt
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:88071. Arnt.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nucleus Source: MGI
    3. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 791790Aryl hydrocarbon receptor nuclear translocatorPRO_0000127119Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei77 – 771PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiP53762.

    PTM databases

    PhosphoSiteiP53762.

    Expressioni

    Tissue specificityi

    Ubiquitous.

    Gene expression databases

    ArrayExpressiP53762.
    BgeeiP53762.
    CleanExiMM_ARNT.
    GenevestigatoriP53762.

    Interactioni

    Subunit structurei

    Heterodimer with HIF1A By similarity. Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. Interacts with NOCA7 and TACC3.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Sim1Q610452EBI-78852,EBI-78890

    Protein-protein interaction databases

    BioGridi198205. 2 interactions.
    DIPiDIP-280N.
    IntActiP53762. 7 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP53762.
    SMRiP53762. Positions 90-465.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini89 – 14254bHLHPROSITE-ProRule annotationAdd
    BLAST
    Domaini161 – 23575PAS 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini349 – 41971PAS 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini424 – 46744PACAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi99 – 1024Poly-Arg
    Compositional biasi503 – 5075Poly-Gln
    Compositional biasi740 – 7434Poly-Ser

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
    Contains 2 PAS (PER-ARNT-SIM) domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG293303.
    GeneTreeiENSGT00650000092935.
    HOGENOMiHOG000234380.
    HOVERGENiHBG000164.
    InParanoidiQ921F3.
    KOiK09097.
    OMAiIVEFCHP.
    OrthoDBiEOG7V1FQ8.
    TreeFamiTF319983.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    IPR001067. Nuc_translocat.
    IPR001610. PAC.
    IPR000014. PAS.
    IPR013767. PAS_fold.
    [Graphical view]
    PfamiPF00010. HLH. 1 hit.
    PF00989. PAS. 1 hit.
    [Graphical view]
    PRINTSiPR00785. NCTRNSLOCATR.
    SMARTiSM00353. HLH. 1 hit.
    SM00086. PAC. 1 hit.
    SM00091. PAS. 2 hits.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    SSF55785. SSF55785. 4 hits.
    TIGRFAMsiTIGR00229. sensory_box. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    PS50112. PAS. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: P53762-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAATTANPEM TSDVPSLGPT IASGNPGPGI QGGGAVVQRA IKRRSGLDFD    50
    DEVEVNTKFL RCDDDQMCND KERFARSDDE QSSADKERLA RENHSEIERR 100
    RRNKMTAYIT ELSDMVPTCS ALARKPDKLT ILRMAVSHMK SLRGTGNTST 150
    DGSYKPSFLT DQELKHLILE AADGFLFIVS CETGRVVYVS DSVTPVLNQP 200
    QSEWFGSTLY DQVHPDDVDK LREQLSTSEN ALTGRVLDLK TGTVKKEGQQ 250
    SSMRMCMGSR RSFICRMRCG TSSVDPVSMN RLSFLRNRCR NGLGSVKEGE 300
    PHFVVVHCTG YIKAWPPAGV SLPDDDPEAG QGSKFCLVAI GRLQVTSSPN 350
    CTDMSNICQP TEFISRHNIE GIFTFVDHRC VATVGYQPQE LLGKNIVEFC 400
    HPEDQQLLRD SFQQVVKLKG QVLSVMFRFR SKTREWLWMR TSSFTFQNPY 450
    SDEIEYIICT NTNVKNSSQE PRPTLSNTIP RSQLGPTANL SLEMGTGQLP 500
    SRQQQQQHTE LDMVPGRDGL ASYNHSQVSV QPVASAGSEH SKPLEKSEGL 550
    FAQDRDPRFP EIYPSITADQ SKGISSSTVP ATQQLFSQGS SFPPNPRPAE 600
    NFRNSGLTPP VTIVQPSSSA GQILAQISRH SNPAQGSAPT WTSSSRPGFA 650
    AQQVPTQATA KTRSSQFGVN NFQTSSSFSA MSLPGAPTAS SGTAAYPALP 700
    NRGSNFPPET GQTTGQFQAR TAEGVGVWPQ WQGQQPHHRS SSSEQHVQQT 750
    QAQAPSQPEV FQEMLSMLGD QSNTYNNEEF PDLTMFPPFS E 791
    Length:791
    Mass (Da):86,963
    Last modified:July 27, 2011 - v3
    Checksum:iBDEC0991CD57D402
    GO
    Isoform Short (identifier: P53762-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         77-100: SDDEQSSADKERLARENHSEIERR → TKFL

    Show »
    Length:771
    Mass (Da):84,613
    Checksum:i1577F629BAE8F38B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti411 – 4111S → T in AAA61732. (PubMed:7961746)Curated
    Sequence conflicti534 – 5341A → R in AAA61732. (PubMed:7961746)Curated
    Sequence conflicti644 – 6441S → T in AAA56717. (PubMed:8065341)Curated
    Sequence conflicti650 – 6501A → C in AAA61732. (PubMed:7961746)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei77 – 10024SDDEQ…EIERR → TKFL in isoform Short. 1 PublicationVSP_002093Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10325 mRNA. Translation: AAA56717.1.
    U14333 mRNA. Translation: AAA61732.1.
    AK153355 mRNA. Translation: BAE31928.1.
    CH466620 Genomic DNA. Translation: EDL38816.1.
    BC012870 mRNA. Translation: AAH12870.1.
    CCDSiCCDS17614.1. [P53762-1]
    PIRiA55448.
    A56241.
    RefSeqiNP_001032826.1. NM_001037737.2. [P53762-1]
    UniGeneiMm.250265.

    Genome annotation databases

    EnsembliENSMUST00000102749; ENSMUSP00000099810; ENSMUSG00000015522. [P53762-1]
    GeneIDi11863.
    KEGGimmu:11863.
    UCSCiuc008qjr.2. mouse. [P53762-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10325 mRNA. Translation: AAA56717.1 .
    U14333 mRNA. Translation: AAA61732.1 .
    AK153355 mRNA. Translation: BAE31928.1 .
    CH466620 Genomic DNA. Translation: EDL38816.1 .
    BC012870 mRNA. Translation: AAH12870.1 .
    CCDSi CCDS17614.1. [P53762-1 ]
    PIRi A55448.
    A56241.
    RefSeqi NP_001032826.1. NM_001037737.2. [P53762-1 ]
    UniGenei Mm.250265.

    3D structure databases

    ProteinModelPortali P53762.
    SMRi P53762. Positions 90-465.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198205. 2 interactions.
    DIPi DIP-280N.
    IntActi P53762. 7 interactions.

    PTM databases

    PhosphoSitei P53762.

    Proteomic databases

    PRIDEi P53762.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000102749 ; ENSMUSP00000099810 ; ENSMUSG00000015522 . [P53762-1 ]
    GeneIDi 11863.
    KEGGi mmu:11863.
    UCSCi uc008qjr.2. mouse. [P53762-1 ]

    Organism-specific databases

    CTDi 405.
    MGIi MGI:88071. Arnt.

    Phylogenomic databases

    eggNOGi NOG293303.
    GeneTreei ENSGT00650000092935.
    HOGENOMi HOG000234380.
    HOVERGENi HBG000164.
    InParanoidi Q921F3.
    KOi K09097.
    OMAi IVEFCHP.
    OrthoDBi EOG7V1FQ8.
    TreeFami TF319983.

    Enzyme and pathway databases

    Reactomei REACT_199107. Regulation of gene expression by Hypoxia-inducible Factor.

    Miscellaneous databases

    ChiTaRSi ARNT. mouse.
    NextBioi 279865.
    PROi P53762.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P53762.
    Bgeei P53762.
    CleanExi MM_ARNT.
    Genevestigatori P53762.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    IPR001067. Nuc_translocat.
    IPR001610. PAC.
    IPR000014. PAS.
    IPR013767. PAS_fold.
    [Graphical view ]
    Pfami PF00010. HLH. 1 hit.
    PF00989. PAS. 1 hit.
    [Graphical view ]
    PRINTSi PR00785. NCTRNSLOCATR.
    SMARTi SM00353. HLH. 1 hit.
    SM00086. PAC. 1 hit.
    SM00091. PAS. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    SSF55785. SSF55785. 4 hits.
    TIGRFAMsi TIGR00229. sensory_box. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    PS50112. PAS. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of functional domains of the aryl hydrocarbon receptor nuclear translocator protein (ARNT)."
      Reisz-Porszasz S., Probst M.R., Fukunaga B.N., Hankinson O.
      Mol. Cell. Biol. 14:6075-6086(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
      Strain: C57BL/6.
      Tissue: Liver.
    2. "Transcriptional activation function of the mouse Ah receptor nuclear translocator."
      Li H., Dong L., Whitlock J.P. Jr.
      J. Biol. Chem. 269:28098-28105(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
      Strain: C57BL/6.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    6. "Identification of a novel mechanism of regulation of Ah (dioxin) receptor function."
      Mimura J., Ema M., Sogawa K., Fujii-Kuriyama Y.
      Genes Dev. 13:20-25(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH AHRR.
    7. "Isolation and characterization of AINT: a novel ARNT interacting protein expressed during murine embryonic development."
      Sadek C.M., Jalaguier S., Feeney E.P., Aitola M., Damdimopoulos A.E., Pelto-Huikko M., Gustafsson J.-A.
      Mech. Dev. 97:13-26(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TACC3.

    Entry informationi

    Entry nameiARNT_MOUSE
    AccessioniPrimary (citable) accession number: P53762
    Secondary accession number(s): Q60661, Q921F3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 134 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3