P53759 (DUS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]
EC=1.3.1.88

Alternative name(s):
tRNA-dihydrouridine synthase 1

Gene names

Name:	DUS1
Ordered Locus Names:	YML080W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U16 and U17 in cytoplasmic tRNAs. Ref.3 Ref.5
Catalytic activity	5,6-dihydrouracil16 in tRNA + NAD(P)+ = uracil16 in tRNA + NAD(P)H. 5,6-dihydrouracil17 in tRNA + NAD(P)+ = uracil17 in tRNA + NAD(P)H.
Cofactor	FAD.
Subunit structure	Monomer.
Sequence similarities	Belongs to the dus family. Dus1 subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nucleus Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro tRNA dihydrouridine synthase activity Inferred from direct assay Ref.3. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RSP5	P39940	2	EBI-27885,EBI-16219

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]		PRO_0000162153

Sequences

	Sequence		Length	Mass (Da)
	P53759 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 7D226E166CE42F43	FASTA	423	48,131
	10 20 30 40 50 60 MTEPALSSAN NALMQKLTGR QLFDKIGRPT RIVAPMVDQS ELAWRILSRR YGATLAYTPM 70 80 90 100 110 120 LHAKLFATSK KYREDNWSSL DGSSVDRPLV VQFCANDPEY LLAAAKLVED KCDAVDLNLG 130 140 150 160 170 180 CPQGIAKKGH YGSFLMEEWD LIHNLINTLH KNLKVPVTAK IRIFDDCEKS LNYAKMVLDA 190 200 210 220 230 240 GAQFLTVHGR VREQKGQKTG LANWETIKYL RDNLPKETVF FANGNILYPE DISRCMEHIG 250 260 270 280 290 300 ADAVMSAEGN LYNPGVFNVG QTKNKEKIFP RVDKIIREYF QIVKECQESK ASKTAMKSHF 310 320 330 340 350 360 FKILRPFLPH HTDIRSTLAT MNAKATWEEW EEQVVKPVEK VVQEIFEQPD IAIKDEITIG 370 380 390 400 410 420 EKQSWGGSYR TVPYWRCQPY FRPVNGITGD KRVMQGLIDE SVNKKRKADV PLESADKKKD VKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA." Xing F., Martzen M.R., Phizicky E.M. RNA 8:370-381(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs." Xing F., Hiley S.L., Hughes T.R., Phizicky E.M. J. Biol. Chem. 279:17850-17860(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46373 Genomic DNA. Translation: CAA86498.1. BK006946 Genomic DNA. Translation: DAA09817.1.
PIR	S48817.
RefSeq	NP_013631.1. NM_001182439.1.
3D structure databases
ProteinModelPortal	P53759.
SMR	P53759. Positions 29-257.
ModBase	Search...
Protein-protein interaction databases
IntAct	P53759. 3 interactions.
MINT	MINT-4083769.
STRING	4932.YML080W.
Proteomic databases
PaxDb	P53759.
PeptideAtlas	P53759.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML080W; YML080W; YML080W.
GeneID	854895.
KEGG	sce:YML080W.
Organism-specific databases
CYGD	YML080w.
SGD	S000004545. DUS1.
Phylogenomic databases
eggNOG	COG0042.
GeneTree	ENSGT00550000075089.
HOGENOM	HOG000203649.
KO	K05542.
OMA	RCQPYFR.
OrthoDB	EOG480N5B.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-16676.
Gene expression databases
Genevestigator	P53759.
GermOnline	YML080W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR001269. tRNA_hU_synthase. IPR018517. tRNA_hU_synthase_CS. [Graphical view]
PANTHER	PTHR11082. PTHR11082. 1 hit.
Pfam	PF01207. Dus. 1 hit. [Graphical view]
PROSITE	PS01136. UPF0034. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977861.

Entry information

Entry name

DUS1_YEAST

Accession

Primary (citable) accession number: P53759
Secondary accession number(s): D6W0K3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program