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P53759

- DUS1_YEAST

UniProt

P53759 - DUS1_YEAST

Protein

tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]

Gene

DUS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U16 and U17 in cytoplasmic tRNAs.2 Publications

    Catalytic activityi

    5,6-dihydrouracil(16) in tRNA + NAD(P)+ = uracil(16) in tRNA + NAD(P)H.
    5,6-dihydrouracil(17) in tRNA + NAD(P)+ = uracil(17) in tRNA + NAD(P)H.

    Cofactori

    FAD.

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. protein binding Source: IntAct
    3. tRNA dihydrouridine synthase activity Source: SGD

    GO - Biological processi

    1. tRNA dihydrouridine synthesis Source: GOC
    2. tRNA modification Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    FAD, Flavoprotein, NAD, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16676.
    YEAST:G3O-32671-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] (EC:1.3.1.88)
    Alternative name(s):
    tRNA-dihydrouridine synthase 1
    Gene namesi
    Name:DUS1
    Ordered Locus Names:YML080W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYML080w.
    SGDiS000004545. DUS1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 423422tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]PRO_0000162153Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylthreonine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP53759.
    PaxDbiP53759.
    PeptideAtlasiP53759.

    Expressioni

    Gene expression databases

    GenevestigatoriP53759.

    Interactioni

    Subunit structurei

    Monomer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RSP5P399402EBI-27885,EBI-16219

    Protein-protein interaction databases

    BioGridi35061. 18 interactions.
    IntActiP53759. 3 interactions.
    MINTiMINT-4083769.
    STRINGi4932.YML080W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53759.
    SMRiP53759. Positions 29-257.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Dus family. Dus1 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0042.
    GeneTreeiENSGT00550000075089.
    HOGENOMiHOG000203649.
    KOiK05542.
    OMAiAQFLTVH.
    OrthoDBiEOG7M6DJ5.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR001269. tRNA_hU_synthase.
    IPR018517. tRNA_hU_synthase_CS.
    [Graphical view]
    PANTHERiPTHR11082. PTHR11082. 1 hit.
    PfamiPF01207. Dus. 1 hit.
    [Graphical view]
    PROSITEiPS01136. UPF0034. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P53759-1 [UniParc]FASTAAdd to Basket

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    MTEPALSSAN NALMQKLTGR QLFDKIGRPT RIVAPMVDQS ELAWRILSRR    50
    YGATLAYTPM LHAKLFATSK KYREDNWSSL DGSSVDRPLV VQFCANDPEY 100
    LLAAAKLVED KCDAVDLNLG CPQGIAKKGH YGSFLMEEWD LIHNLINTLH 150
    KNLKVPVTAK IRIFDDCEKS LNYAKMVLDA GAQFLTVHGR VREQKGQKTG 200
    LANWETIKYL RDNLPKETVF FANGNILYPE DISRCMEHIG ADAVMSAEGN 250
    LYNPGVFNVG QTKNKEKIFP RVDKIIREYF QIVKECQESK ASKTAMKSHF 300
    FKILRPFLPH HTDIRSTLAT MNAKATWEEW EEQVVKPVEK VVQEIFEQPD 350
    IAIKDEITIG EKQSWGGSYR TVPYWRCQPY FRPVNGITGD KRVMQGLIDE 400
    SVNKKRKADV PLESADKKKD VKA 423
    Length:423
    Mass (Da):48,131
    Last modified:October 1, 1996 - v1
    Checksum:i7D226E166CE42F43
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46373 Genomic DNA. Translation: CAA86498.1.
    BK006946 Genomic DNA. Translation: DAA09817.1.
    PIRiS48817.
    RefSeqiNP_013631.1. NM_001182439.1.

    Genome annotation databases

    EnsemblFungiiYML080W; YML080W; YML080W.
    GeneIDi854895.
    KEGGisce:YML080W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46373 Genomic DNA. Translation: CAA86498.1 .
    BK006946 Genomic DNA. Translation: DAA09817.1 .
    PIRi S48817.
    RefSeqi NP_013631.1. NM_001182439.1.

    3D structure databases

    ProteinModelPortali P53759.
    SMRi P53759. Positions 29-257.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35061. 18 interactions.
    IntActi P53759. 3 interactions.
    MINTi MINT-4083769.
    STRINGi 4932.YML080W.

    Proteomic databases

    MaxQBi P53759.
    PaxDbi P53759.
    PeptideAtlasi P53759.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML080W ; YML080W ; YML080W .
    GeneIDi 854895.
    KEGGi sce:YML080W.

    Organism-specific databases

    CYGDi YML080w.
    SGDi S000004545. DUS1.

    Phylogenomic databases

    eggNOGi COG0042.
    GeneTreei ENSGT00550000075089.
    HOGENOMi HOG000203649.
    KOi K05542.
    OMAi AQFLTVH.
    OrthoDBi EOG7M6DJ5.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-16676.
    YEAST:G3O-32671-MONOMER.

    Miscellaneous databases

    NextBioi 977861.
    PROi P53759.

    Gene expression databases

    Genevestigatori P53759.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR001269. tRNA_hU_synthase.
    IPR018517. tRNA_hU_synthase_CS.
    [Graphical view ]
    PANTHERi PTHR11082. PTHR11082. 1 hit.
    Pfami PF01207. Dus. 1 hit.
    [Graphical view ]
    PROSITEi PS01136. UPF0034. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA."
      Xing F., Martzen M.R., Phizicky E.M.
      RNA 8:370-381(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. Cited for: PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs."
      Xing F., Hiley S.L., Hughes T.R., Phizicky E.M.
      J. Biol. Chem. 279:17850-17860(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiDUS1_YEAST
    AccessioniPrimary (citable) accession number: P53759
    Secondary accession number(s): D6W0K3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Uncharacterized protein families (UPF)
      List of uncharacterized protein family (UPF) entries
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3