Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P53759 (DUS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]

EC=1.3.1.88
Alternative name(s):
tRNA-dihydrouridine synthase 1
Gene names
Name:DUS1
Ordered Locus Names:YML080W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U16 and U17 in cytoplasmic tRNAs. Ref.3 Ref.5

Catalytic activity

5,6-dihydrouracil16 in tRNA + NAD(P)+ = uracil16 in tRNA + NAD(P)H.

5,6-dihydrouracil17 in tRNA + NAD(P)+ = uracil17 in tRNA + NAD(P)H.

Cofactor

FAD.

Subunit structure

Monomer.

Sequence similarities

Belongs to the Dus family. Dus1 subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RSP5P399402EBI-27885,EBI-16219

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6
Chain2 – 423422tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]
PRO_0000162153

Amino acid modifications

Modified residue21N-acetylthreonine Ref.6

Sequences

Sequence LengthMass (Da)Tools
P53759 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 7D226E166CE42F43

FASTA42348,131
        10         20         30         40         50         60 
MTEPALSSAN NALMQKLTGR QLFDKIGRPT RIVAPMVDQS ELAWRILSRR YGATLAYTPM 

        70         80         90        100        110        120 
LHAKLFATSK KYREDNWSSL DGSSVDRPLV VQFCANDPEY LLAAAKLVED KCDAVDLNLG 

       130        140        150        160        170        180 
CPQGIAKKGH YGSFLMEEWD LIHNLINTLH KNLKVPVTAK IRIFDDCEKS LNYAKMVLDA 

       190        200        210        220        230        240 
GAQFLTVHGR VREQKGQKTG LANWETIKYL RDNLPKETVF FANGNILYPE DISRCMEHIG 

       250        260        270        280        290        300 
ADAVMSAEGN LYNPGVFNVG QTKNKEKIFP RVDKIIREYF QIVKECQESK ASKTAMKSHF 

       310        320        330        340        350        360 
FKILRPFLPH HTDIRSTLAT MNAKATWEEW EEQVVKPVEK VVQEIFEQPD IAIKDEITIG 

       370        380        390        400        410        420 
EKQSWGGSYR TVPYWRCQPY FRPVNGITGD KRVMQGLIDE SVNKKRKADV PLESADKKKD 


VKA 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA."
Xing F., Martzen M.R., Phizicky E.M.
RNA 8:370-381(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs."
Xing F., Hiley S.L., Hughes T.R., Phizicky E.M.
J. Biol. Chem. 279:17850-17860(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46373 Genomic DNA. Translation: CAA86498.1.
BK006946 Genomic DNA. Translation: DAA09817.1.
PIRS48817.
RefSeqNP_013631.1. NM_001182439.1.

3D structure databases

ProteinModelPortalP53759.
SMRP53759. Positions 29-257.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35061. 18 interactions.
IntActP53759. 3 interactions.
MINTMINT-4083769.
STRING4932.YML080W.

Proteomic databases

MaxQBP53759.
PaxDbP53759.
PeptideAtlasP53759.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML080W; YML080W; YML080W.
GeneID854895.
KEGGsce:YML080W.

Organism-specific databases

CYGDYML080w.
SGDS000004545. DUS1.

Phylogenomic databases

eggNOGCOG0042.
GeneTreeENSGT00550000075089.
HOGENOMHOG000203649.
KOK05542.
OMAAQFLTVH.
OrthoDBEOG7M6DJ5.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-16676.
YEAST:G3O-32671-MONOMER.

Gene expression databases

GenevestigatorP53759.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
[Graphical view]
PANTHERPTHR11082. PTHR11082. 1 hit.
PfamPF01207. Dus. 1 hit.
[Graphical view]
PROSITEPS01136. UPF0034. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977861.
PROP53759.

Entry information

Entry nameDUS1_YEAST
AccessionPrimary (citable) accession number: P53759
Secondary accession number(s): D6W0K3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Uncharacterized protein families (UPF)

List of uncharacterized protein family (UPF) entries

SIMILARITY comments

Index of protein domains and families