Reviewed, UniProtKB/Swiss-Prot P53759 (DUS1_YEAST)

Names and origin

Protein names

Recommended name:
    tRNA-dihydrouridine synthase 1
    EC=1.-.-.-

Gene names

Name:	DUS1
Ordered Locus Names:	YML080W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U16 and U17 in cytoplasmic tRNAs. Ref.2 Ref.4
Cofactor	FAD.
Subunit structure	Monomer.
Sequence similarities	Belongs to the dus family. Dus1 subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tRNA modification Ref.2 Ref.4 Inferred from direct assay. Source: SGD
Cellular component	nucleus Inferred from direct assay. Source: SGD
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct tRNA dihydrouridine synthase activity Ref.2 Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P43582	1	EBI-27885,EBI-22766
RSP5	P39940	1	EBI-27885,EBI-16219
VID30	P53076	1	EBI-27885,EBI-24173

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	tRNA-dihydrouridine synthase 1		PRO_0000162153

Sequences

	Sequence		Length	Mass (Da)
	P53759-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 7D226E166CE42F43	FASTA	423	48,131
	10 20 30 40 50 60 MTEPALSSAN NALMQKLTGR QLFDKIGRPT RIVAPMVDQS ELAWRILSRR YGATLAYTPM 70 80 90 100 110 120 LHAKLFATSK KYREDNWSSL DGSSVDRPLV VQFCANDPEY LLAAAKLVED KCDAVDLNLG 130 140 150 160 170 180 CPQGIAKKGH YGSFLMEEWD LIHNLINTLH KNLKVPVTAK IRIFDDCEKS LNYAKMVLDA 190 200 210 220 230 240 GAQFLTVHGR VREQKGQKTG LANWETIKYL RDNLPKETVF FANGNILYPE DISRCMEHIG 250 260 270 280 290 300 ADAVMSAEGN LYNPGVFNVG QTKNKEKIFP RVDKIIREYF QIVKECQESK ASKTAMKSHF 310 320 330 340 350 360 FKILRPFLPH HTDIRSTLAT MNAKATWEEW EEQVVKPVEK VVQEIFEQPD IAIKDEITIG 370 380 390 400 410 420 EKQSWGGSYR TVPYWRCQPY FRPVNGITGD KRVMQGLIDE SVNKKRKADV PLESADKKKD VKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA." Xing F., Martzen M.R., Phizicky E.M. RNA 8:370-381(2002) [PubMed: 12003496] [Abstract] Cited for: FUNCTION.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs." Xing F., Hiley S.L., Hughes T.R., Phizicky E.M. J. Biol. Chem. 279:17850-17860(2004) [PubMed: 14970222] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z46373 Genomic DNA. Translation: CAA86498.1.
PIR	S48817.
RefSeq	NP_013631.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P53759. 5 interactions.
STRING	P53759.
Proteomic databases
PeptideAtlas	P53759.
Genome annotation databases
Ensembl	YML080W; YML080W; YML080W; Saccharomyces cerevisiae. [Genome view]
GeneID	854895.
KEGG	sce:YML080W.
NMPDR	fig|4932.3.peg.4668.
Organism-specific databases
CYGD	YML080w.
SGD	S000004545. DUS1.
Phylogenomic databases
HOGENOM	P53759.
OMA	RCQPYFR
OrthoDB	EOG9CJX0F
Gene expression databases
ArrayExpress	P53759.
Genevestigator	P53759.
GermOnline	YML080W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR001269. tRNA_hU_synthase. IPR018517. tRNA_hU_synthase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11082. Du_synth. 1 hit.
Pfam	PF01207. Dus. 1 hit. [Graphical view]
PROSITE	PS01136. UPF0034. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	977861.

Entry information

Entry name

DUS1_YEAST

Accession

Primary (citable) accession number: P53759

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 24, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)