Reviewed, UniProtKB/Swiss-Prot P53753 (ENG1_YEAST)

Names and origin

Protein names

Recommended name:
    Endo-1,3(4)-beta-glucanase 1
      Short name=Endo-1,4-beta-glucanase 1
      Short name=Endo-1,3-beta-glucanase 1
    EC=3.2.1.6
Alternative name(s):
    Laminarinase-1
    Daughter specific expression protein 4

Gene names

Name:	DSE4
Synonyms:	ENG1
Ordered Locus Names:	YNR067C
ORF Names:	N3547

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1117 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the dissoultion of the mother-daughter septum during cell separation. Ref.2
Catalytic activity	Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.
Subcellular location	Secreted › cell wall. Note: Localizes asymmetrically to the daughter side of the septum. Ref.2
Post-translational modification	Glycosylated. Ref.2
Miscellaneous	Present with 64 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the glycosyl hydrolase 81 family.

Ontologies

Keywords
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytokinesis, completion of separation Inferred from mutant phenotype. Source: SGD
Cellular component	cell septum Ref.2 Inferred from direct assay. Source: SGD extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell fungal-type cell wall Inferred from direct assay. Source: SGD
Molecular function	endo-1,3(4)-beta-glucanase activity Inferred from electronic annotation. Source: EC glucan endo-1,3-beta-D-glucosidase activity Ref.2 Inferred from genetic interaction. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 1117	1101	Endo-1,3(4)-beta-glucanase 1		PRO_0000012133
Regions
	Compositional bias	342 – 345	4	Poly-Ser
	Compositional bias	365 – 370	6	Poly-Ser
	Compositional bias	376 – 383	8	Poly-Ser
Amino acid modifications
	Glycosylation	138	1	N-linked (GlcNAc...) Potential
	Glycosylation	186	1	N-linked (GlcNAc...) Potential
	Glycosylation	223	1	N-linked (GlcNAc...) Potential
	Glycosylation	259	1	N-linked (GlcNAc...) Potential
	Glycosylation	280	1	N-linked (GlcNAc...) Potential
	Glycosylation	303	1	N-linked (GlcNAc...) Potential
	Glycosylation	307	1	N-linked (GlcNAc...) Potential
	Glycosylation	393	1	N-linked (GlcNAc...) Potential
	Glycosylation	533	1	N-linked (GlcNAc...) Potential
	Glycosylation	886	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P53753-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 87F13A07E42B0AD1	FASTA	1,117	121,064
	10 20 30 40 50 60 MQLYLTLLFL LSFVECSYIS FISNNADEIL ETDLIETLSY ATLTVGEPYV AQSVVVTRVS 70 80 90 100 110 120 AASHSPLSVS PKNRVSASPI NSQDSDSNTR TAVQLSLSLS NYASQVSQKI SAQTNNDPVT 130 140 150 160 170 180 VSNIYANDNS KSKSSVHNLS SVSGVASVMP SASTMRKVTT LLSQTASTST STLFSSSLSI 190 200 210 220 230 240 SGTQLNGTLL TSVSKGTIDP LVTQMPSYSS QETKIIPSSL TSNKTIYTIS VRTNAATATG 250 260 270 280 290 300 EDSFIASTPA SSTLFYPSNS TQDLVQTLAS TTASPAYPSN RTQITLSPSV SLYSTTSPIY 310 320 330 340 350 360 PSNITENGSS PSPSLSSTVS PVYPSSSTGN ILLSSLFSTV DSSSSPVSST LDTIYVSSSM 370 380 390 400 410 420 QATISSSSSS RQTKTSSSSL STSTSSTATT TENSSTTTIV NLFNAVSTDE PPTVFDRSPN 430 440 450 460 470 480 PMSLADGVSN DGPIQTNKFY TNLIVGSQES PAFVYPYSLW KYTSSSYGFA VQHTTVDQYS 490 500 510 520 530 540 YGGYDSSGNA EYLVNPLGIA HVVFSASNFD SSMTMQVDEM TLSSTRVVLS ESNDSSNYLE 550 560 570 580 590 600 IPLVQGMGFA TGIYHGSLNA KIGSSVGFNT IVSESSSNLA QGILKYRITL LNGVTWLCYV 610 620 630 640 650 660 IGPDDLTSTD FSLEVSSEYE IKASASVDGL IIQLAVAPSE TDYEVFYDQA AGMYVTNFKL 670 680 690 700 710 720 QGVSDGSTAT YEFSYTTQGE SASGSTMIFA LPHHESSFSD IMQDYYTGIQ LASTTKGVMN 730 740 750 760 770 780 GYLTTSLQFS TSLNRQISWL PWSSQLGSNL LEYSKEQLQL LAEVANSELQ VSISESISGL 790 800 810 820 830 840 NTYYLGKVID KYSYILLTVS EIIQDEASTK STLENIKSAF DILLQNEQTY PLIYDTKFNG 850 860 870 880 890 900 LVSSGDWGST STQYDFGNTY YNDHHFHYGY IIHAAAVIGY VDSKLNGTWA ADNKDWVNSL 910 920 930 940 950 960 VRDVANPSEK DEYFAQSRMF DWFNGHSWAA GLYENGNGKN EESSSEDYNF AYAMKLWGAT 970 980 990 1000 1010 1020 IGDQSMELRG DLMISIMKDA MNDYFYYQND NTVEPEEIIG NKVSGILFDN IIDYTTYFGT 1030 1040 1050 1060 1070 1080 NTEYIHGIHM LPITPVSSNI RSETFVEEEW QTKIEPIIES IESGWTGILK LNQALFDPVD 1090 1100 1110 SYAFFSDSTF DSSTYLDNGM SRTWALAFSG GLANSIA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Eng1p, an endo-1,3-beta-glucanase localized at the daughter side of the septum, is involved in cell separation in Saccharomyces cerevisiae." Baladron V., Ufano S., Duenas E., Martin-Cuadrado A.B., del Rey F., Vazquez de Aldana C.R. Eukaryot. Cell 1:774-786(2002) [PubMed: 12455695] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z71682 Genomic DNA. Translation: CAA96349.1.
PIR	S63399.
RefSeq	NP_014465.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P53753. 3 interactions.
Protein family/group databases
CAZy	GH81. Glycoside Hydrolase Family 81.
Proteomic databases
PeptideAtlas	P53753.
Genome annotation databases
Ensembl	YNR067C. Saccharomyces cerevisiae. [Contig view]
GeneID	855804.
GenomeReviews	Gene locus YNR067C in contig Y13139_GR.
KEGG	sce:YNR067C.
NMPDR	fig|4932.3.peg.5546.
Organism-specific databases
CYGD	YNR067c.
SGD	S000005350. DSE4.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P53753.
OMA	P53753. IRDVANP.
Enzyme and pathway databases
BRENDA	3.2.1.6. 250.
Gene expression databases
ArrayExpress	P53753.
GermOnline	YNR067C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005200. Glyco_hydro_81. [Graphical view]
Pfam	PF03639. Glyco_hydro_81. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	980318.

Entry information

Entry name

ENG1_YEAST

Accession

Primary (citable) accession number: P53753

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)