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P53753

- ENG1_YEAST

UniProt

P53753 - ENG1_YEAST

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Protein

Endo-1,3(4)-beta-glucanase 1

Gene

DSE4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the dissoultion of the mother-daughter septum during cell separation.1 Publication

Catalytic activityi

Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.

GO - Molecular functioni

  1. glucan endo-1,3-beta-D-glucosidase activity Source: SGD
  2. glucan endo-1,3-beta-glucanase activity, C-3 substituted reducing group Source: UniProtKB-EC
  3. glucan endo-1,4-beta-glucanase activity, C-3 substituted reducing group Source: UniProtKB-EC

GO - Biological processi

  1. cell wall macromolecule catabolic process Source: InterPro
  2. cytokinesis, completion of separation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciYEAST:G3O-33371-MONOMER.

Protein family/group databases

CAZyiGH81. Glycoside Hydrolase Family 81.
mycoCLAPiLAM81A_YEAST.

Names & Taxonomyi

Protein namesi
Recommended name:
Endo-1,3(4)-beta-glucanase 1 (EC:3.2.1.6)
Short name:
Endo-1,3-beta-glucanase 1
Short name:
Endo-1,4-beta-glucanase 1
Alternative name(s):
Daughter specific expression protein 4
Laminarinase-1
Gene namesi
Name:DSE4
Synonyms:ENG1
Ordered Locus Names:YNR067C
ORF Names:N3547
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNR067c.
SGDiS000005350. DSE4.

Subcellular locationi

Secretedcell wall 1 Publication
Note: Localizes asymmetrically to the daughter side of the septum.

GO - Cellular componenti

  1. cell septum Source: SGD
  2. extracellular region Source: SGD
  3. fungal-type cell wall Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence AnalysisAdd
BLAST
Chaini17 – 11171101Endo-1,3(4)-beta-glucanase 1PRO_0000012133Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi138 – 1381N-linked (GlcNAc...)Sequence Analysis
Glycosylationi186 – 1861N-linked (GlcNAc...)Sequence Analysis
Glycosylationi223 – 2231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi259 – 2591N-linked (GlcNAc...)Sequence Analysis
Glycosylationi280 – 2801N-linked (GlcNAc...)Sequence Analysis
Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence Analysis
Glycosylationi307 – 3071N-linked (GlcNAc...)Sequence Analysis
Glycosylationi393 – 3931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi533 – 5331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi886 – 8861N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP53753.
PaxDbiP53753.
PeptideAtlasiP53753.

Expressioni

Gene expression databases

GenevestigatoriP53753.

Interactioni

Protein-protein interaction databases

BioGridi35893. 11 interactions.
IntActiP53753. 1 interaction.
MINTiMINT-2786281.
STRINGi4932.YNR067C.

Structurei

3D structure databases

ProteinModelPortaliP53753.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi342 – 3454Poly-Ser
Compositional biasi365 – 3706Poly-Ser
Compositional biasi376 – 3838Poly-Ser

Sequence similaritiesi

Belongs to the glycosyl hydrolase 81 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5498.
GeneTreeiENSGT00530000069141.
HOGENOMiHOG000211468.
InParanoidiP53753.
OMAiGMKLWGN.
OrthoDBiEOG7MSMXB.

Family and domain databases

InterProiIPR005200. Endo-beta-glucanase.
[Graphical view]
PfamiPF03639. Glyco_hydro_81. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53753-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQLYLTLLFL LSFVECSYIS FISNNADEIL ETDLIETLSY ATLTVGEPYV
60 70 80 90 100
AQSVVVTRVS AASHSPLSVS PKNRVSASPI NSQDSDSNTR TAVQLSLSLS
110 120 130 140 150
NYASQVSQKI SAQTNNDPVT VSNIYANDNS KSKSSVHNLS SVSGVASVMP
160 170 180 190 200
SASTMRKVTT LLSQTASTST STLFSSSLSI SGTQLNGTLL TSVSKGTIDP
210 220 230 240 250
LVTQMPSYSS QETKIIPSSL TSNKTIYTIS VRTNAATATG EDSFIASTPA
260 270 280 290 300
SSTLFYPSNS TQDLVQTLAS TTASPAYPSN RTQITLSPSV SLYSTTSPIY
310 320 330 340 350
PSNITENGSS PSPSLSSTVS PVYPSSSTGN ILLSSLFSTV DSSSSPVSST
360 370 380 390 400
LDTIYVSSSM QATISSSSSS RQTKTSSSSL STSTSSTATT TENSSTTTIV
410 420 430 440 450
NLFNAVSTDE PPTVFDRSPN PMSLADGVSN DGPIQTNKFY TNLIVGSQES
460 470 480 490 500
PAFVYPYSLW KYTSSSYGFA VQHTTVDQYS YGGYDSSGNA EYLVNPLGIA
510 520 530 540 550
HVVFSASNFD SSMTMQVDEM TLSSTRVVLS ESNDSSNYLE IPLVQGMGFA
560 570 580 590 600
TGIYHGSLNA KIGSSVGFNT IVSESSSNLA QGILKYRITL LNGVTWLCYV
610 620 630 640 650
IGPDDLTSTD FSLEVSSEYE IKASASVDGL IIQLAVAPSE TDYEVFYDQA
660 670 680 690 700
AGMYVTNFKL QGVSDGSTAT YEFSYTTQGE SASGSTMIFA LPHHESSFSD
710 720 730 740 750
IMQDYYTGIQ LASTTKGVMN GYLTTSLQFS TSLNRQISWL PWSSQLGSNL
760 770 780 790 800
LEYSKEQLQL LAEVANSELQ VSISESISGL NTYYLGKVID KYSYILLTVS
810 820 830 840 850
EIIQDEASTK STLENIKSAF DILLQNEQTY PLIYDTKFNG LVSSGDWGST
860 870 880 890 900
STQYDFGNTY YNDHHFHYGY IIHAAAVIGY VDSKLNGTWA ADNKDWVNSL
910 920 930 940 950
VRDVANPSEK DEYFAQSRMF DWFNGHSWAA GLYENGNGKN EESSSEDYNF
960 970 980 990 1000
AYAMKLWGAT IGDQSMELRG DLMISIMKDA MNDYFYYQND NTVEPEEIIG
1010 1020 1030 1040 1050
NKVSGILFDN IIDYTTYFGT NTEYIHGIHM LPITPVSSNI RSETFVEEEW
1060 1070 1080 1090 1100
QTKIEPIIES IESGWTGILK LNQALFDPVD SYAFFSDSTF DSSTYLDNGM
1110
SRTWALAFSG GLANSIA
Length:1,117
Mass (Da):121,064
Last modified:October 1, 1996 - v1
Checksum:i87F13A07E42B0AD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71682 Genomic DNA. Translation: CAA96349.1.
BK006947 Genomic DNA. Translation: DAA10608.1.
PIRiS63399.
RefSeqiNP_014465.1. NM_001183244.1.

Genome annotation databases

EnsemblFungiiYNR067C; YNR067C; YNR067C.
GeneIDi855804.
KEGGisce:YNR067C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71682 Genomic DNA. Translation: CAA96349.1 .
BK006947 Genomic DNA. Translation: DAA10608.1 .
PIRi S63399.
RefSeqi NP_014465.1. NM_001183244.1.

3D structure databases

ProteinModelPortali P53753.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35893. 11 interactions.
IntActi P53753. 1 interaction.
MINTi MINT-2786281.
STRINGi 4932.YNR067C.

Protein family/group databases

CAZyi GH81. Glycoside Hydrolase Family 81.
mycoCLAPi LAM81A_YEAST.

Proteomic databases

MaxQBi P53753.
PaxDbi P53753.
PeptideAtlasi P53753.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNR067C ; YNR067C ; YNR067C .
GeneIDi 855804.
KEGGi sce:YNR067C.

Organism-specific databases

CYGDi YNR067c.
SGDi S000005350. DSE4.

Phylogenomic databases

eggNOGi COG5498.
GeneTreei ENSGT00530000069141.
HOGENOMi HOG000211468.
InParanoidi P53753.
OMAi GMKLWGN.
OrthoDBi EOG7MSMXB.

Enzyme and pathway databases

BioCyci YEAST:G3O-33371-MONOMER.

Miscellaneous databases

NextBioi 980318.

Gene expression databases

Genevestigatori P53753.

Family and domain databases

InterProi IPR005200. Endo-beta-glucanase.
[Graphical view ]
Pfami PF03639. Glyco_hydro_81. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Eng1p, an endo-1,3-beta-glucanase localized at the daughter side of the septum, is involved in cell separation in Saccharomyces cerevisiae."
    Baladron V., Ufano S., Duenas E., Martin-Cuadrado A.B., del Rey F., Vazquez de Aldana C.R.
    Eukaryot. Cell 1:774-786(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiENG1_YEAST
AccessioniPrimary (citable) accession number: P53753
Secondary accession number(s): D6W1P2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 64 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3