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P53753

- ENG1_YEAST

UniProt

P53753 - ENG1_YEAST

Protein

Endo-1,3(4)-beta-glucanase 1

Gene

DSE4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Involved in the dissoultion of the mother-daughter septum during cell separation.1 Publication

    Catalytic activityi

    Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.

    GO - Molecular functioni

    1. glucan endo-1,3-beta-D-glucosidase activity Source: SGD
    2. glucan endo-1,3-beta-glucanase activity, C-3 substituted reducing group Source: UniProtKB-EC
    3. glucan endo-1,4-beta-glucanase activity, C-3 substituted reducing group Source: UniProtKB-EC

    GO - Biological processi

    1. cell wall macromolecule catabolic process Source: InterPro
    2. cytokinesis, completion of separation Source: SGD

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33371-MONOMER.

    Protein family/group databases

    CAZyiGH81. Glycoside Hydrolase Family 81.
    mycoCLAPiLAM81A_YEAST.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endo-1,3(4)-beta-glucanase 1 (EC:3.2.1.6)
    Short name:
    Endo-1,3-beta-glucanase 1
    Short name:
    Endo-1,4-beta-glucanase 1
    Alternative name(s):
    Daughter specific expression protein 4
    Laminarinase-1
    Gene namesi
    Name:DSE4
    Synonyms:ENG1
    Ordered Locus Names:YNR067C
    ORF Names:N3547
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNR067c.
    SGDiS000005350. DSE4.

    Subcellular locationi

    Secretedcell wall 1 Publication
    Note: Localizes asymmetrically to the daughter side of the septum.

    GO - Cellular componenti

    1. cell septum Source: SGD
    2. extracellular region Source: SGD
    3. fungal-type cell wall Source: SGD

    Keywords - Cellular componenti

    Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Sequence AnalysisAdd
    BLAST
    Chaini17 – 11171101Endo-1,3(4)-beta-glucanase 1PRO_0000012133Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi138 – 1381N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi186 – 1861N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi223 – 2231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi259 – 2591N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi280 – 2801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi307 – 3071N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi393 – 3931N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi533 – 5331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi886 – 8861N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP53753.
    PaxDbiP53753.
    PeptideAtlasiP53753.

    Expressioni

    Gene expression databases

    GenevestigatoriP53753.

    Interactioni

    Protein-protein interaction databases

    BioGridi35893. 11 interactions.
    IntActiP53753. 1 interaction.
    MINTiMINT-2786281.
    STRINGi4932.YNR067C.

    Structurei

    3D structure databases

    ProteinModelPortaliP53753.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi342 – 3454Poly-Ser
    Compositional biasi365 – 3706Poly-Ser
    Compositional biasi376 – 3838Poly-Ser

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 81 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5498.
    GeneTreeiENSGT00530000069141.
    HOGENOMiHOG000211468.
    OMAiGMKLWGN.
    OrthoDBiEOG7MSMXB.

    Family and domain databases

    InterProiIPR005200. Glyco_hydro_81.
    [Graphical view]
    PfamiPF03639. Glyco_hydro_81. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P53753-1 [UniParc]FASTAAdd to Basket

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    MQLYLTLLFL LSFVECSYIS FISNNADEIL ETDLIETLSY ATLTVGEPYV     50
    AQSVVVTRVS AASHSPLSVS PKNRVSASPI NSQDSDSNTR TAVQLSLSLS 100
    NYASQVSQKI SAQTNNDPVT VSNIYANDNS KSKSSVHNLS SVSGVASVMP 150
    SASTMRKVTT LLSQTASTST STLFSSSLSI SGTQLNGTLL TSVSKGTIDP 200
    LVTQMPSYSS QETKIIPSSL TSNKTIYTIS VRTNAATATG EDSFIASTPA 250
    SSTLFYPSNS TQDLVQTLAS TTASPAYPSN RTQITLSPSV SLYSTTSPIY 300
    PSNITENGSS PSPSLSSTVS PVYPSSSTGN ILLSSLFSTV DSSSSPVSST 350
    LDTIYVSSSM QATISSSSSS RQTKTSSSSL STSTSSTATT TENSSTTTIV 400
    NLFNAVSTDE PPTVFDRSPN PMSLADGVSN DGPIQTNKFY TNLIVGSQES 450
    PAFVYPYSLW KYTSSSYGFA VQHTTVDQYS YGGYDSSGNA EYLVNPLGIA 500
    HVVFSASNFD SSMTMQVDEM TLSSTRVVLS ESNDSSNYLE IPLVQGMGFA 550
    TGIYHGSLNA KIGSSVGFNT IVSESSSNLA QGILKYRITL LNGVTWLCYV 600
    IGPDDLTSTD FSLEVSSEYE IKASASVDGL IIQLAVAPSE TDYEVFYDQA 650
    AGMYVTNFKL QGVSDGSTAT YEFSYTTQGE SASGSTMIFA LPHHESSFSD 700
    IMQDYYTGIQ LASTTKGVMN GYLTTSLQFS TSLNRQISWL PWSSQLGSNL 750
    LEYSKEQLQL LAEVANSELQ VSISESISGL NTYYLGKVID KYSYILLTVS 800
    EIIQDEASTK STLENIKSAF DILLQNEQTY PLIYDTKFNG LVSSGDWGST 850
    STQYDFGNTY YNDHHFHYGY IIHAAAVIGY VDSKLNGTWA ADNKDWVNSL 900
    VRDVANPSEK DEYFAQSRMF DWFNGHSWAA GLYENGNGKN EESSSEDYNF 950
    AYAMKLWGAT IGDQSMELRG DLMISIMKDA MNDYFYYQND NTVEPEEIIG 1000
    NKVSGILFDN IIDYTTYFGT NTEYIHGIHM LPITPVSSNI RSETFVEEEW 1050
    QTKIEPIIES IESGWTGILK LNQALFDPVD SYAFFSDSTF DSSTYLDNGM 1100
    SRTWALAFSG GLANSIA 1117
    Length:1,117
    Mass (Da):121,064
    Last modified:October 1, 1996 - v1
    Checksum:i87F13A07E42B0AD1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71682 Genomic DNA. Translation: CAA96349.1.
    BK006947 Genomic DNA. Translation: DAA10608.1.
    PIRiS63399.
    RefSeqiNP_014465.1. NM_001183244.1.

    Genome annotation databases

    EnsemblFungiiYNR067C; YNR067C; YNR067C.
    GeneIDi855804.
    KEGGisce:YNR067C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71682 Genomic DNA. Translation: CAA96349.1 .
    BK006947 Genomic DNA. Translation: DAA10608.1 .
    PIRi S63399.
    RefSeqi NP_014465.1. NM_001183244.1.

    3D structure databases

    ProteinModelPortali P53753.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35893. 11 interactions.
    IntActi P53753. 1 interaction.
    MINTi MINT-2786281.
    STRINGi 4932.YNR067C.

    Protein family/group databases

    CAZyi GH81. Glycoside Hydrolase Family 81.
    mycoCLAPi LAM81A_YEAST.

    Proteomic databases

    MaxQBi P53753.
    PaxDbi P53753.
    PeptideAtlasi P53753.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNR067C ; YNR067C ; YNR067C .
    GeneIDi 855804.
    KEGGi sce:YNR067C.

    Organism-specific databases

    CYGDi YNR067c.
    SGDi S000005350. DSE4.

    Phylogenomic databases

    eggNOGi COG5498.
    GeneTreei ENSGT00530000069141.
    HOGENOMi HOG000211468.
    OMAi GMKLWGN.
    OrthoDBi EOG7MSMXB.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33371-MONOMER.

    Miscellaneous databases

    NextBioi 980318.

    Gene expression databases

    Genevestigatori P53753.

    Family and domain databases

    InterProi IPR005200. Glyco_hydro_81.
    [Graphical view ]
    Pfami PF03639. Glyco_hydro_81. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Eng1p, an endo-1,3-beta-glucanase localized at the daughter side of the septum, is involved in cell separation in Saccharomyces cerevisiae."
      Baladron V., Ufano S., Duenas E., Martin-Cuadrado A.B., del Rey F., Vazquez de Aldana C.R.
      Eukaryot. Cell 1:774-786(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiENG1_YEAST
    AccessioniPrimary (citable) accession number: P53753
    Secondary accession number(s): D6W1P2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 64 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3