Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P53753 (ENG1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endo-1,3(4)-beta-glucanase 1

Short name=Endo-1,3-beta-glucanase 1
Short name=Endo-1,4-beta-glucanase 1
EC=3.2.1.6
Alternative name(s):
Daughter specific expression protein 4
Laminarinase-1
Gene names
Name:DSE4
Synonyms:ENG1
Ordered Locus Names:YNR067C
ORF Names:N3547
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1117 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the dissoultion of the mother-daughter septum during cell separation. Ref.3

Catalytic activity

Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.

Subcellular location

Secretedcell wall. Note: Localizes asymmetrically to the daughter side of the septum. Ref.3

Post-translational modification

Glycosylated. Ref.3

Miscellaneous

Present with 64 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the glycosyl hydrolase 81 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 Potential
Chain17 – 11171101Endo-1,3(4)-beta-glucanase 1
PRO_0000012133

Regions

Compositional bias342 – 3454Poly-Ser
Compositional bias365 – 3706Poly-Ser
Compositional bias376 – 3838Poly-Ser

Amino acid modifications

Glycosylation1381N-linked (GlcNAc...) Potential
Glycosylation1861N-linked (GlcNAc...) Potential
Glycosylation2231N-linked (GlcNAc...) Potential
Glycosylation2591N-linked (GlcNAc...) Potential
Glycosylation2801N-linked (GlcNAc...) Potential
Glycosylation3031N-linked (GlcNAc...) Potential
Glycosylation3071N-linked (GlcNAc...) Potential
Glycosylation3931N-linked (GlcNAc...) Potential
Glycosylation5331N-linked (GlcNAc...) Potential
Glycosylation8861N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P53753 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 87F13A07E42B0AD1

FASTA1,117121,064
        10         20         30         40         50         60 
MQLYLTLLFL LSFVECSYIS FISNNADEIL ETDLIETLSY ATLTVGEPYV AQSVVVTRVS 

        70         80         90        100        110        120 
AASHSPLSVS PKNRVSASPI NSQDSDSNTR TAVQLSLSLS NYASQVSQKI SAQTNNDPVT 

       130        140        150        160        170        180 
VSNIYANDNS KSKSSVHNLS SVSGVASVMP SASTMRKVTT LLSQTASTST STLFSSSLSI 

       190        200        210        220        230        240 
SGTQLNGTLL TSVSKGTIDP LVTQMPSYSS QETKIIPSSL TSNKTIYTIS VRTNAATATG 

       250        260        270        280        290        300 
EDSFIASTPA SSTLFYPSNS TQDLVQTLAS TTASPAYPSN RTQITLSPSV SLYSTTSPIY 

       310        320        330        340        350        360 
PSNITENGSS PSPSLSSTVS PVYPSSSTGN ILLSSLFSTV DSSSSPVSST LDTIYVSSSM 

       370        380        390        400        410        420 
QATISSSSSS RQTKTSSSSL STSTSSTATT TENSSTTTIV NLFNAVSTDE PPTVFDRSPN 

       430        440        450        460        470        480 
PMSLADGVSN DGPIQTNKFY TNLIVGSQES PAFVYPYSLW KYTSSSYGFA VQHTTVDQYS 

       490        500        510        520        530        540 
YGGYDSSGNA EYLVNPLGIA HVVFSASNFD SSMTMQVDEM TLSSTRVVLS ESNDSSNYLE 

       550        560        570        580        590        600 
IPLVQGMGFA TGIYHGSLNA KIGSSVGFNT IVSESSSNLA QGILKYRITL LNGVTWLCYV 

       610        620        630        640        650        660 
IGPDDLTSTD FSLEVSSEYE IKASASVDGL IIQLAVAPSE TDYEVFYDQA AGMYVTNFKL 

       670        680        690        700        710        720 
QGVSDGSTAT YEFSYTTQGE SASGSTMIFA LPHHESSFSD IMQDYYTGIQ LASTTKGVMN 

       730        740        750        760        770        780 
GYLTTSLQFS TSLNRQISWL PWSSQLGSNL LEYSKEQLQL LAEVANSELQ VSISESISGL 

       790        800        810        820        830        840 
NTYYLGKVID KYSYILLTVS EIIQDEASTK STLENIKSAF DILLQNEQTY PLIYDTKFNG 

       850        860        870        880        890        900 
LVSSGDWGST STQYDFGNTY YNDHHFHYGY IIHAAAVIGY VDSKLNGTWA ADNKDWVNSL 

       910        920        930        940        950        960 
VRDVANPSEK DEYFAQSRMF DWFNGHSWAA GLYENGNGKN EESSSEDYNF AYAMKLWGAT 

       970        980        990       1000       1010       1020 
IGDQSMELRG DLMISIMKDA MNDYFYYQND NTVEPEEIIG NKVSGILFDN IIDYTTYFGT 

      1030       1040       1050       1060       1070       1080 
NTEYIHGIHM LPITPVSSNI RSETFVEEEW QTKIEPIIES IESGWTGILK LNQALFDPVD 

      1090       1100       1110 
SYAFFSDSTF DSSTYLDNGM SRTWALAFSG GLANSIA 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Eng1p, an endo-1,3-beta-glucanase localized at the daughter side of the septum, is involved in cell separation in Saccharomyces cerevisiae."
Baladron V., Ufano S., Duenas E., Martin-Cuadrado A.B., del Rey F., Vazquez de Aldana C.R.
Eukaryot. Cell 1:774-786(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z71682 Genomic DNA. Translation: CAA96349.1.
BK006947 Genomic DNA. Translation: DAA10608.1.
PIRS63399.
RefSeqNP_014465.1. NM_001183244.1.

3D structure databases

ProteinModelPortalP53753.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35893. 11 interactions.
IntActP53753. 1 interaction.
MINTMINT-2786281.
STRING4932.YNR067C.

Protein family/group databases

CAZyGH81. Glycoside Hydrolase Family 81.
mycoCLAPLAM81A_YEAST.

Proteomic databases

PaxDbP53753.
PeptideAtlasP53753.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNR067C; YNR067C; YNR067C.
GeneID855804.
KEGGsce:YNR067C.

Organism-specific databases

CYGDYNR067c.
SGDS000005350. DSE4.

Phylogenomic databases

eggNOGCOG5498.
GeneTreeENSGT00530000069141.
HOGENOMHOG000211468.
OMAHPEYVHG.
OrthoDBEOG7MSMXB.

Enzyme and pathway databases

BioCycYEAST:G3O-33371-MONOMER.

Gene expression databases

GenevestigatorP53753.

Family and domain databases

InterProIPR005200. Glyco_hydro_81.
[Graphical view]
PfamPF03639. Glyco_hydro_81. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio980318.

Entry information

Entry nameENG1_YEAST
AccessionPrimary (citable) accession number: P53753
Secondary accession number(s): D6W1P2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 19, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries