P53747 (YN8Z_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized vacuolar membrane protein YNR061C

Gene names

Ordered Locus Names:	YNR061C
ORF Names:	N3523

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Vacuole membrane; Multi-pass membrane protein Ref.4.
Miscellaneous	Present with 339 molecules/cell in log phase SD medium.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	fungal-type vacuole membrane Inferred from direct assay PubMed 22842922. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	Uncharacterized vacuolar membrane protein YNR061C		PRO_0000203481
Regions
	Topological domain	1 – 15	15	Cytoplasmic Potential
	Transmembrane	16 – 36	21	Helical; Potential
	Topological domain	37 – 41	5	Vacuolar Potential
	Transmembrane	42 – 62	21	Helical; Potential
	Topological domain	63	1	Cytoplasmic Potential
	Transmembrane	64 – 84	21	Helical; Potential
	Topological domain	85 – 116	32	Vacuolar Potential
	Transmembrane	117 – 137	21	Helical; Potential
	Topological domain	138 – 219	82	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P53747 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 744FABCD688BD3FC	FASTA	219	24,317
	10 20 30 40 50 60 MLKLTTTSVT FHVLRYFQLG LSVTNLLLAS FAIITNYKVD RILRLSLAVS IISSVYFGIV 70 80 90 100 110 120 RFLPVLLIFV MEIVQTVLWF TAFVTLASKF GSMSCSSMPR GINFDYSGSC KIAKIDILPE 130 140 150 160 170 180 AVLFILFLAT TYASYITVLS QAKENGSSTR SVLKACVKAL RDTVDRLETS LEESEPLLDL 190 200 210 EVQEDARTET ESIEDSTDSE DNANIEQEKV IDGSIEHSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z71676 Genomic DNA. Translation: CAA96343.1. AY558009 Genomic DNA. Translation: AAS56335.1. BK006947 Genomic DNA. Translation: DAA10602.1.
PIR	S63393.
RefSeq	NP_014459.1. NM_001183238.1.
3D structure databases
ProteinModelPortal	P53747.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4462N.
MINT	MINT-531956.
Proteomic databases
PaxDb	P53747.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNR061C; YNR061C; YNR061C.
GeneID	855798.
KEGG	sce:YNR061C.
Organism-specific databases
CYGD	YNR061c.
SGD	S000005344. YNR061C.
Phylogenomic databases
eggNOG	NOG309382.
OrthoDB	EOG4PRX1H.
Gene expression databases
Genevestigator	P53747.
GermOnline	YNR061C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR021128. MARVEL-like_dom. [Graphical view]
Pfam	PF01284. MARVEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980300.

Entry information

Entry name

YN8Z_YEAST

Accession

Primary (citable) accession number: P53747
Secondary accession number(s): D6W1N6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program