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Protein

Ferric reductase transmembrane component 4

Gene

FRE4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Siderophore-iron reductase responsible for reducing extracellular iron prior to import. Catalyzes the reductive uptake of Fe3+ bound to dihydroxamate rhodotorulic acid. Fe3+ is reduced to Fe2+, which then dissociates from the siderophore and can be imported by the high-affinity Fe2+ transport complex in the plasma membrane.1 Publication

Catalytic activityi

2 Fe(II)-siderophore + NADP+ + H+ = 2 Fe(III)-siderophore + NADPH.

Cofactori

FADCurated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi309Iron (heme 1 axial ligand)By similarity1
Metal bindingi323Iron (heme 2 axial ligand)By similarity1
Metal bindingi379Iron (heme 1 axial ligand)By similarity1
Metal bindingi393Iron (heme 2 axial ligand)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi472 – 478FADSequence analysis7
Nucleotide bindingi519 – 522NADPSequence analysis4
Nucleotide bindingi685 – 686NADPSequence analysis2

GO - Molecular functioni

  • ferric-chelate reductase (NADPH) activity Source: UniProtKB-EC
  • ferric-chelate reductase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellular iron ion homeostasis Source: GO_Central
  • copper ion import Source: GO_Central
  • iron ion transport Source: GO_Central
  • siderophore transport Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Ion transport, Iron transport, Transport

Keywords - Ligandi

FAD, Flavoprotein, Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciYEAST:G3O-33364-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ferric reductase transmembrane component 4 (EC:1.16.1.9)
Alternative name(s):
Ferric-chelate reductase 4
Gene namesi
Name:FRE4
Ordered Locus Names:YNR060W
ORF Names:N3518
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNR060W.
SGDiS000005343. FRE4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 156ExtracellularBy similarityAdd BLAST138
Transmembranei157 – 177Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini178 – 228CytoplasmicBy similarityAdd BLAST51
Transmembranei229 – 249Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini250 – 267ExtracellularBy similarityAdd BLAST18
Transmembranei268 – 288Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini289 – 304CytoplasmicBy similarityAdd BLAST16
Transmembranei305 – 325Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini326 – 346ExtracellularBy similarityAdd BLAST21
Transmembranei347 – 367Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini368 – 373CytoplasmicBy similarity6
Transmembranei374 – 394Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini395ExtracellularBy similarity1
Transmembranei396 – 416Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini417 – 719CytoplasmicBy similarityAdd BLAST303

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000001014019 – 719Ferric reductase transmembrane component 4Add BLAST701

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi51N-linked (GlcNAc...)Sequence analysis1
Glycosylationi80N-linked (GlcNAc...)Sequence analysis1
Glycosylationi101N-linked (GlcNAc...)Sequence analysis1
Glycosylationi113N-linked (GlcNAc...)Sequence analysis1
Glycosylationi127N-linked (GlcNAc...)Sequence analysis1
Glycosylationi135N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP53746.

PTM databases

iPTMnetiP53746.

Expressioni

Inductioni

By iron deprivation.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi35886. 13 interactors.
DIPiDIP-7626N.
IntActiP53746. 1 interactor.
MINTiMINT-1354495.

Structurei

3D structure databases

ProteinModelPortaliP53746.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini273 – 407Ferric oxidoreductaseAdd BLAST135
Domaini408 – 527FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST120

Sequence similaritiesi

Belongs to the ferric reductase (FRE) family.Curated
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000007891.
HOGENOMiHOG000000805.
InParanoidiP53746.
KOiK00521.
OMAiINTHERR.
OrthoDBiEOG092C04QA.

Family and domain databases

InterProiIPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53746-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLVHIISFL LFFQLSAAKA PPSKTSLINT HERRSIYSCY VGLRKETWGF
60 70 80 90 100
NGSAICRYEP AIQSMLYCLY EDTHEKGYSN KTLEKGFEEM RQFCYTPKFL
110 120 130 140 150
NMTDAEFYTS LDNGTYYIQD QPKAGINITY PIRLNTTLRK AYYDAYYGYY
160 170 180 190 200
YNHDIPYYFG GIICAYFVGV MLLAGLIRFL NYTPIKKIMF QQKLVNYVRG
210 220 230 240 250
YTTLPTLYEK HAEPFSYLKV ITGYLPTRFE TLVILGYLIL HTIFMAYKYQ
260 270 280 290 300
YDPYHIIFAA HRAEVAHFVA YRSGILSFAH LPLIVLFAGR NNFLQLISGL
310 320 330 340 350
KHTSFIVFHK WLGRMMFLDA IIHAAGFTNY YLYYKKWNTV RLRVYWKFGI
360 370 380 390 400
ATTCLAGMLI FFSIAAFRRH YYETFMALHI VFAALFLYTC WEHVTNFSGI
410 420 430 440 450
EWIYAAIAIW GVDRIVRITR IALLGFPKAD LQLVGSDLVR VTVKKPKKFW
460 470 480 490 500
KAKPGQYVFV SFLRPLCFWQ SHPFTVMDSC VNDRELVIVL KAKKGVTKLV
510 520 530 540 550
RNFVERKGGK ASMRLAIEGP YGSKSTAHRF DNVLLLAGGS GLPGPISHAL
560 570 580 590 600
ELGKTTAASG KNFVQLVIAV RGLDMLNACK KELMALKGLN VQVHIYNSKQ
610 620 630 640 650
ELASAEKISS NEVKNGETTA EKAPSSLSNS EKAPSESENT ELPLSLNDTS
660 670 680 690 700
ISDLEFATFH VGRPNVEEIL NESVNHSGSL AVVCCGPPIF VDTARNQTAK
710
AVIRNPSRMI EYLEEYQAW
Length:719
Mass (Da):82,015
Last modified:October 1, 1996 - v1
Checksum:i9CA91F1F890AF1F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71675 Genomic DNA. Translation: CAA96342.1.
BK006947 Genomic DNA. Translation: DAA10601.1.
PIRiS63392.
RefSeqiNP_014458.1. NM_001183237.1.

Genome annotation databases

EnsemblFungiiYNR060W; YNR060W; YNR060W.
GeneIDi855797.
KEGGisce:YNR060W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71675 Genomic DNA. Translation: CAA96342.1.
BK006947 Genomic DNA. Translation: DAA10601.1.
PIRiS63392.
RefSeqiNP_014458.1. NM_001183237.1.

3D structure databases

ProteinModelPortaliP53746.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35886. 13 interactors.
DIPiDIP-7626N.
IntActiP53746. 1 interactor.
MINTiMINT-1354495.

PTM databases

iPTMnetiP53746.

Proteomic databases

PRIDEiP53746.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNR060W; YNR060W; YNR060W.
GeneIDi855797.
KEGGisce:YNR060W.

Organism-specific databases

EuPathDBiFungiDB:YNR060W.
SGDiS000005343. FRE4.

Phylogenomic databases

GeneTreeiENSGT00390000007891.
HOGENOMiHOG000000805.
InParanoidiP53746.
KOiK00521.
OMAiINTHERR.
OrthoDBiEOG092C04QA.

Enzyme and pathway databases

BioCyciYEAST:G3O-33364-MONOMER.

Miscellaneous databases

PROiP53746.

Family and domain databases

InterProiIPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFRE4_YEAST
AccessioniPrimary (citable) accession number: P53746
Secondary accession number(s): D6W1N5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.