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Protein

Probable alpha-1,3-mannosyltransferase MNT4

Gene

MNT4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

GO - Molecular functioni

  • alpha-1,3-mannosyltransferase activity Source: SGD

GO - Biological processi

  • protein O-linked glycosylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-33363-MONOMER.

Protein family/group databases

CAZyiGT71. Glycosyltransferase Family 71.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alpha-1,3-mannosyltransferase MNT4 (EC:2.4.1.-)
Gene namesi
Name:MNT4
Ordered Locus Names:YNR059W
ORF Names:N3514
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNR059W.
SGDiS000005342. MNT4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1010CytoplasmicSequence analysis
Transmembranei11 – 2919Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini30 – 580551LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 580580Probable alpha-1,3-mannosyltransferase MNT4PRO_0000080561Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi132 – 1321N-linked (GlcNAc...)Sequence analysis
Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence analysis
Glycosylationi223 – 2231N-linked (GlcNAc...)Sequence analysis
Glycosylationi349 – 3491N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP53745.

Interactioni

Protein-protein interaction databases

BioGridi35885. 12 interactions.
DIPiDIP-5262N.
IntActiP53745. 1 interaction.
MINTiMINT-529118.

Structurei

3D structure databases

ProteinModelPortaliP53745.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MNN1/MNT family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000003622.
HOGENOMiHOG000113620.
InParanoidiP53745.
OMAiYTWCAYS.
OrthoDBiEOG7PS1Q8.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
InterProiIPR022751. Alpha_mannosyltransferase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF11051. Mannosyl_trans3. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.

Sequencei

Sequence statusi: Complete.

P53745-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLRIRRIKK LAPLIFTSLL SLIVLFRVYR QYPFSDHFET RREDDRSGNV
60 70 80 90 100
HCFSRLRQIE EYEKPELTSK FYEPNRWKSF ISYVTRSRKD VKTVSRSLSN
110 120 130 140 150
LDLYQKCSKE IRADQDISLL HSIETKLFPY INFTALNSEQ SHNFWPVHTR
160 170 180 190 200
FDGTKYRGQV LQFSSENNSF IGTSPIEFKA SEPFWENWLN SALQRNSKGV
210 220 230 240 250
VMSVSEYLVA DTIRLIRVLR LLNNSLPIEI VHKSDLHESS QQLLIAAARE
260 270 280 290 300
SGSLNYPPQE LWFLDVKDML NDEYLARFKR FSNKWLAITF CSFQIPIFLD
310 320 330 340 350
SDTVPFVPLD TLYEIDGFKR TGTLFFKDRS FPTSKLSPLQ VKVLKQIINN
360 370 380 390 400
SLDVSSDSEQ GFEILKHNLN DEMAIDAIEA LIFKKQKHYM DSGLVIFDKQ
410 420 430 440 450
KHFFCLPIAI MLQFSPIQEF FHGDKEWFWL SLFISKKEFT FYPIEASNVG
460 470 480 490 500
RLEKPETLES STICSTQLSH TDVYGNLLWL NGGLSVCKKN CWNYDFTKRK
510 520 530 540 550
EIAAKYKSVD ELRNYYQSPV KLEAVIIPDV SKSPWSQQSE CVMYSYCTHY
560 570 580
RKGQYGKLIE FTDSQKKYYE KVVELWNKVV
Length:580
Mass (Da):68,082
Last modified:October 1, 1996 - v1
Checksum:iF94C53283887EC62
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501V → L in AAB63969 (PubMed:10333520).Curated
Sequence conflicti87 – 871S → G in AAB63969 (PubMed:10333520).Curated
Sequence conflicti134 – 1341T → A in AAB63969 (PubMed:10333520).Curated
Sequence conflicti206 – 2061E → D in AAB63969 (PubMed:10333520).Curated
Sequence conflicti254 – 26714LNYPP…FLDVK → PGTELELRPQFTG in AAB63969 (PubMed:10333520).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71674 Genomic DNA. Translation: CAA96341.1.
U53467 Genomic DNA. Translation: AAB63969.1.
BK006947 Genomic DNA. Translation: DAA10600.1.
PIRiS63391.
RefSeqiNP_014457.3. NM_001183236.3.

Genome annotation databases

EnsemblFungiiYNR059W; YNR059W; YNR059W.
GeneIDi855796.
KEGGisce:YNR059W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71674 Genomic DNA. Translation: CAA96341.1.
U53467 Genomic DNA. Translation: AAB63969.1.
BK006947 Genomic DNA. Translation: DAA10600.1.
PIRiS63391.
RefSeqiNP_014457.3. NM_001183236.3.

3D structure databases

ProteinModelPortaliP53745.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35885. 12 interactions.
DIPiDIP-5262N.
IntActiP53745. 1 interaction.
MINTiMINT-529118.

Protein family/group databases

CAZyiGT71. Glycosyltransferase Family 71.

Proteomic databases

MaxQBiP53745.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNR059W; YNR059W; YNR059W.
GeneIDi855796.
KEGGisce:YNR059W.

Organism-specific databases

EuPathDBiFungiDB:YNR059W.
SGDiS000005342. MNT4.

Phylogenomic databases

GeneTreeiENSGT00390000003622.
HOGENOMiHOG000113620.
InParanoidiP53745.
OMAiYTWCAYS.
OrthoDBiEOG7PS1Q8.

Enzyme and pathway databases

BioCyciYEAST:G3O-33363-MONOMER.

Miscellaneous databases

PROiP53745.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
InterProiIPR022751. Alpha_mannosyltransferase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF11051. Mannosyl_trans3. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Characterization of the biotin biosynthesis pathway in Saccharomyces cerevisiae and evidence for a cluster containing BIO5, a novel gene involved in vitamer uptake."
    Phalip V., Kuhn I., Lemoine Y., Jeltsch J.-M.
    Gene 232:43-51(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-267.
    Strain: ATCC 28383 / FL100 / VTT C-80102.

Entry informationi

Entry nameiMNT4_YEAST
AccessioniPrimary (citable) accession number: P53745
Secondary accession number(s): D6W1N4, Q03127
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.