P53745 (MNT4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable alpha-1,3-mannosyltransferase MNT4
EC=2.4.1.-

Gene names

Name:	MNT4
Ordered Locus Names:	YNR059W
ORF Names:	N3514

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	580 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subcellular location	Membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the MNN1/MNT family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein O-linked glycosylation Inferred from direct assay. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,3-mannosyltransferase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 580	580	Probable alpha-1,3-mannosyltransferase MNT4		PRO_0000080561
Regions
	Topological domain	1 – 10	10	Cytoplasmic Potential
	Transmembrane	11 – 29	19	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	30 – 580	551	Lumenal Potential
Amino acid modifications
	Glycosylation	132	1	N-linked (GlcNAc...) Potential
	Glycosylation	167	1	N-linked (GlcNAc...) Potential
	Glycosylation	223	1	N-linked (GlcNAc...) Potential
	Glycosylation	349	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	50	1	V → L in AAB63969. Ref.3
	Sequence conflict	87	1	S → G in AAB63969. Ref.3
	Sequence conflict	134	1	T → A in AAB63969. Ref.3
	Sequence conflict	206	1	E → D in AAB63969. Ref.3
	Sequence conflict	254 – 267	14	LNYPP…FLDVK → PGTELELRPQFTG in AAB63969. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P53745 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: F94C53283887EC62	FASTA	580	68,082
	10 20 30 40 50 60 MVLRIRRIKK LAPLIFTSLL SLIVLFRVYR QYPFSDHFET RREDDRSGNV HCFSRLRQIE 70 80 90 100 110 120 EYEKPELTSK FYEPNRWKSF ISYVTRSRKD VKTVSRSLSN LDLYQKCSKE IRADQDISLL 130 140 150 160 170 180 HSIETKLFPY INFTALNSEQ SHNFWPVHTR FDGTKYRGQV LQFSSENNSF IGTSPIEFKA 190 200 210 220 230 240 SEPFWENWLN SALQRNSKGV VMSVSEYLVA DTIRLIRVLR LLNNSLPIEI VHKSDLHESS 250 260 270 280 290 300 QQLLIAAARE SGSLNYPPQE LWFLDVKDML NDEYLARFKR FSNKWLAITF CSFQIPIFLD 310 320 330 340 350 360 SDTVPFVPLD TLYEIDGFKR TGTLFFKDRS FPTSKLSPLQ VKVLKQIINN SLDVSSDSEQ 370 380 390 400 410 420 GFEILKHNLN DEMAIDAIEA LIFKKQKHYM DSGLVIFDKQ KHFFCLPIAI MLQFSPIQEF 430 440 450 460 470 480 FHGDKEWFWL SLFISKKEFT FYPIEASNVG RLEKPETLES STICSTQLSH TDVYGNLLWL 490 500 510 520 530 540 NGGLSVCKKN CWNYDFTKRK EIAAKYKSVD ELRNYYQSPV KLEAVIIPDV SKSPWSQQSE 550 560 570 580 CVMYSYCTHY RKGQYGKLIE FTDSQKKYYE KVVELWNKVV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Characterization of the biotin biosynthesis pathway in Saccharomyces cerevisiae and evidence for a cluster containing BIO5, a novel gene involved in vitamer uptake." Phalip V., Kuhn I., Lemoine Y., Jeltsch J.-M. Gene 232:43-51(1999) [PubMed: 10333520] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-267. Strain: ATCC 28383 / FL100 / VTT C-80102.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z71674 Genomic DNA. Translation: CAA96341.1. U53467 Genomic DNA. Translation: AAB63969.1. BK006947 Genomic DNA. Translation: DAA10600.1.
PIR	S63391.
RefSeq	NP_014457.1. NM_001183236.1.
3D structure databases
ProteinModelPortal	P53745.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5262N.
MINT	MINT-529118.
STRING	P53745.
Protein family/group databases
CAZy	GT71. Glycosyltransferase Family 71.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNR059W; YNR059W; YNR059W.
GeneID	855796.
KEGG	sce:YNR059W.
NMPDR	fig|4932.3.peg.5538.
Organism-specific databases
CYGD	YNR059w.
SGD	S000005342. MNT4.
Phylogenomic databases
eggNOG	fuNOG10625.
GeneTree	EFGT00050000003823.
HOGENOM	HBG204460.
OMA	YTWCAYS.
OrthoDB	EOG42NN87.
Gene expression databases
ArrayExpress	P53745.
Genevestigator	P53745.
GermOnline	YNR059W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022751. Alpha_mannosyltransferase. [Graphical view]
Pfam	PF11051. Mannosyl_trans3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980294.

Entry information

Entry name

MNT4_YEAST

Accession

Primary (citable) accession number: P53745
Secondary accession number(s): D6W1N4, Q03127

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program