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Protein

Pre-rRNA-processing protein ESF2

Gene

ESF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the small subunit (SSU) processome assembly and function, and in the 18S rRNA synthesis. Required for the early cleavages at sites A0, A1 and A2. Stimulates DBP8 RNA helicase ATPase activity.2 Publications

Miscellaneous

Present with 12800 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • ATPase activator activity Source: SGD
  • RNA binding Source: SGD

GO - Biological processi

  • endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • small-subunit processome assembly Source: SGD

Keywordsi

Molecular functionRNA-binding
Biological processRibosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-33360-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-rRNA-processing protein ESF2
Alternative name(s):
18S rRNA factor 2
Gene namesi
Name:ESF2
Synonyms:ABT1
Ordered Locus Names:YNR054C
ORF Names:N3491
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNR054C.
SGDiS000005337. ESF2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002034801 – 316Pre-rRNA-processing protein ESF2Add BLAST316

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7PhosphoserineCombined sources1
Modified residuei13PhosphoserineCombined sources1
Modified residuei14PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53743.
PRIDEiP53743.

PTM databases

iPTMnetiP53743.

Interactioni

Subunit structurei

Component of the 90S pre-ribosomes. Interacts directly with DBP8.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ESF1Q063443EBI-28537,EBI-34121

Protein-protein interaction databases

BioGridi35879. 152 interactors.
DIPiDIP-6417N.
IntActiP53743. 13 interactors.
MINTiMINT-618016.
STRINGi4932.YNR054C.

Structurei

3D structure databases

ProteinModelPortaliP53743.
SMRiP53743.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini114 – 204RRMAdd BLAST91

Sequence similaritiesi

Belongs to the ESF2/ABP1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000002062.
HOGENOMiHOG000242500.
InParanoidiP53743.
KOiK14785.
OMAiWHNLTEQ.
OrthoDBiEOG092C5VYU.

Family and domain databases

CDDicd12263. RRM_ABT1_like. 1 hit.
InterProiView protein in InterPro
IPR034353. ABT1/ESF2_RRM.
IPR035979. RBD_domain_sf.
SUPFAMiSSF54928. SSF54928. 1 hit.

Sequencei

Sequence statusi: Complete.

P53743-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKVNSDFE DFSSDEETDQ HNVLIQTKKK ISSKDDIFSK KVEDIESENE
60 70 80 90 100
SDIEEEQKQE EKEDVEQPDK ENGEKLDREV EEQASSTTSL DLKTEKLRQL
110 120 130 140 150
VKSKAAKKSK HKTGVVYFSS IPPYMKPAKM RQILTRFGEV DRLFLKKEDD
160 170 180 190 200
QKYKQRVKGG GNKKNKYEEG WAEFIRKRDA KLCAETLNGN IIGGKKGTFY
210 220 230 240 250
HDDILNVKYL PGFKWADLTE QIARENDIRQ AKLEMEISQA NKLNAEFIRN
260 270 280 290 300
VEQSKMIQNI KNSRKRAGKE GESADSHPHR EFKQRRVETS RANAPSDIKQ
310
QSSGSKDLGN VLTNLL
Length:316
Mass (Da):36,408
Last modified:October 1, 1996 - v1
Checksum:i5F38B88CDEEFC01F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71669 Genomic DNA. Translation: CAA96335.1.
BK006947 Genomic DNA. Translation: DAA10595.1.
PIRiS63385.
RefSeqiNP_014452.1. NM_001183231.1.

Genome annotation databases

EnsemblFungiiYNR054C; YNR054C; YNR054C.
GeneIDi855790.
KEGGisce:YNR054C.

Similar proteinsi

Entry informationi

Entry nameiESF2_YEAST
AccessioniPrimary (citable) accession number: P53743
Secondary accession number(s): D6W1M9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 25, 2017
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names