P53741 (BRE5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UBP3-associated protein BRE5

Alternative name(s):
Brefeldin-A sensitivity protein 5

Gene names

Name:	BRE5
Ordered Locus Names:	YNR051C
ORF Names:	N3465

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has a role in de-ubiquitination. In conjunction with UBP3, cleaves ubiquitin, leading to the subsequent mono-ubiquitination of sec23. Ref.6
Subunit structure	Interacts with UBP3. Ref.6
Miscellaneous	Present with 11700 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Contains 1 NTF2 domain. Contains 1 RRM (RNA recognition motif) domain.
Sequence caution	The sequence AAA34833.1 differs from that shown. Reason: Frameshift at positions 317 and 320. The sequence AAA34834.1 differs from that shown. Reason: Frameshift at positions 317 and 320.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein deubiquitination Inferred from mutant phenotype Ref.6. Source: SGD regulation of ER to Golgi vesicle-mediated transport Inferred from mutant phenotype Ref.6. Source: SGD regulation of retrograde vesicle-mediated transport, Golgi to ER Inferred from mutant phenotype. Source: SGD ribophagy Inferred from mutant phenotype. Source: SGD transport Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
UBP3	Q01477	5	EBI-28528,EBI-19834

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 515

515

UBP3-associated protein BRE5

PRO_0000194803

Regions

Domain

8 – 140

133

NTF2

Domain

418 – 494

77

RRM

Amino acid modifications

Modified residue

187

1

Phosphoserine Ref.9 Ref.10

Modified residue

278

1

Phosphothreonine Ref.10 Ref.11

Modified residue

282

1

Phosphoserine Ref.10 Ref.11

Modified residue

336

1

Phosphothreonine Ref.5 Ref.8 Ref.11

Modified residue

338

1

Phosphoserine Ref.11

Modified residue

339

1

Phosphoserine Ref.5 Ref.11

Modified residue

340

1

Phosphoserine Ref.11

Modified residue

398

1

Phosphoserine Ref.9

Secondary structure

1

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.

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515

Helix Strand Turn

Details...

Helix

5 – 22

18

Helix

24 – 30

7

Beta strand

31 – 40

10

Beta strand

56 – 61

6

Helix

62 – 71

10

Helix

73 – 77

5

Beta strand

79 – 92

14

Helix

93 – 95

3

Beta strand

97 – 111

15

Beta strand

114 – 124

11

Beta strand

131 – 140

10

Sequences

	Sequence		Length	Mass (Da)
	P53741 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 462A7D4309A69192	FASTA	515	57,674
	10 20 30 40 50 60 MGVTVQDICF AFLQNYYERM RTDPSKLAYF YASTAELTHT NYQSKSTNEK DDVLPTVKVT 70 80 90 100 110 120 GRENINKFFS RNDAKVRSLK LKLDTIDFQY TGHLHKSILI MATGEMFWTG TPVYKFCQTF 130 140 150 160 170 180 ILLPSSNGST FDITNDIIRF ISNSFKPYVL TDASLSQSNE ENSVSAVEED KIRHESGVEK 190 200 210 220 230 240 EKEKEKSPEI SKPKAKKETV KDTTAPTESS TQEKPIVDHS QPRAIPVTKE SKIHTETVPS 250 260 270 280 290 300 STKGNHKQDE VSTEELGNVT KLNEKSHKAE KKAAPIKTKE GSVEAINAVN NSSLPNGKEV 310 320 330 340 350 360 SDEKPVPGGV KEAETEIKPI EPQVSDAKES GNNASTPSSS PEPVANPPKM TWASKLMNEN 370 380 390 400 410 420 SDRISKNNTT VEYIRPETLP KKPTERKFEM GNRRDNASAN SKNKKKPVFS TVNKDGFYPI 430 440 450 460 470 480 YIRGTNGLRE EKLRSALEKE FGKVMRITAA DNFAVVDFET QKSQIDALEK KKKSIDGIEV 490 500 510 CLERKTVKKP TSNNPPGIFT NGTRSHRKQP LKRKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Cusick M.E. Submitted (MAR-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-441.
[5]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed: 11875433] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-336 AND SER-339, MASS SPECTROMETRY. Strain: 2124.
[6]	"Ubp3 requires a cofactor, Bre5, to specifically de-ubiquitinate the COPII protein, Sec23." Cohen M., Stutz F., Belgareh N., Haguenauer-Tsapis R., Dargemont C. Nat. Cell Biol. 5:661-667(2003) [PubMed: 12778054] [Abstract] Cited for: FUNCTION, SUBUNIT.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-336, MASS SPECTROMETRY. Strain: ADR376.
[9]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-187 AND SER-398, MASS SPECTROMETRY.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-187; THR-278 AND SER-282, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-278; SER-282; THR-336; SER-338; SER-339 AND SER-340, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z71666 Genomic DNA. Translation: CAA96332.1.
AY692784 Genomic DNA. Translation: AAT92803.1.
M88607 Genomic DNA. Translation: AAA34833.1. Frameshift.
M88607 Genomic DNA. Translation: AAA34834.1. Frameshift.
BK006947 Genomic DNA. Translation: DAA10592.1.

PIR

S63382.

RefSeq

NP_014449.1. NM_001183228.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZX2	X-ray	2.10	A/B	1-146	[»]
2QIY	X-ray	1.69	A/B	1-146	[»]

ProteinModelPortal

P53741.

SMR

P53741. Positions 3-143, 427-471.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-4254N.

IntAct

P53741. 16 interactions.

MINT

MINT-483528.

STRING

P53741.

Proteomic databases

PeptideAtlas

P53741.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YNR051C; YNR051C; YNR051C.

GeneID

855787.

KEGG

sce:YNR051C.

NMPDR

fig|4932.3.peg.5530.

Organism-specific databases

CYGD

YNR051c.

SGD

S000005334. BRE5.

Phylogenomic databases

eggNOG

fuNOG10366.

OMA

TAELTHV.

OrthoDB

EOG4N33ZJ.

Gene expression databases

ArrayExpress

P53741.

Genevestigator

P53741.

GermOnline

YNR051C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR002075. NTF2.
IPR018222. Nuclear_transport_factor_2_euk.
IPR000504. RRM_dom.
[Graphical view]

Pfam

PF02136. NTF2. 1 hit.
[Graphical view]

SMART

SM00360. RRM. 1 hit.
[Graphical view]

PROSITE

PS50177. NTF2_DOMAIN. 1 hit.
PS50102. RRM. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

980268.

Entry information

Entry name

BRE5_YEAST

Accession

Primary (citable) accession number: P53741
Secondary accession number(s): D6W1M6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program