P53740 (YN8S_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized protein YNR048W

Gene names

Ordered Locus Names:	YNR048W
ORF Names:	N3453

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Membrane; Multi-pass membrane protein.
Miscellaneous	Present with 4510 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the CDC50/LEM3 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to drug Inferred from mutant phenotype PubMed 12842877. Source: SGD
Cellular_component	integral to membrane Inferred from sequence or structural similarity PubMed 15919184. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Uncharacterized protein YNR048W		PRO_0000207672
Regions
	Topological domain	1 – 46	46	Cytoplasmic Potential
	Transmembrane	47 – 67	21	Helical; Potential
	Topological domain	68 – 334	267	Extracellular Potential
	Transmembrane	335 – 355	21	Helical; Potential
	Topological domain	356 – 393	38	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	78	1	N-linked (GlcNAc...) Potential
	Glycosylation	123	1	N-linked (GlcNAc...) Potential
	Glycosylation	187	1	N-linked (GlcNAc...) Potential
	Glycosylation	202	1	N-linked (GlcNAc...) Potential
	Glycosylation	213	1	N-linked (GlcNAc...) Potential
	Glycosylation	240	1	N-linked (GlcNAc...) Potential
	Glycosylation	291	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P53740 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 9BCA199AA3D23969	FASTA	393	44,542
	10 20 30 40 50 60 MGLILRWKEK KQLSSKQNAQ KSRKPANTSF RQQRLKAWQP ILSPQSVLPL LILMACVFAP 70 80 90 100 110 120 IGIGLVVSTI SVQRLVVNYT ECDALAPAKH FETIPSEYVD YHFSKKVAVQ PQWMVLTDPE 130 140 150 160 170 180 LGNQTCRIQF EVPNHIKKST YVYYRLTNFN QNYREYVQSL DLDQLKGKAL IGNDLDPNCD 190 200 210 220 230 240 PLRTVENKTI FPCGLIANSM FNDTFGTTLT GVNDTADYLL TTKGIAWDTD SHRYGKTEYN 250 260 270 280 290 300 ASDIVPPPNW AKLFPNGYTD DNIPDLQNWE QFKIWMRTAA LPNFYKLAMK NETNGLGKGI 310 320 330 340 350 360 YIADIELNYP VRSFYGTKSF VLTTNSIIGA GNEALGIVYL IVAGIATLFA ILFLIKVIFK 370 380 390 PRPMHDHSYL NFENSDTPFD ESSVVSIPLR EIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z71663 Genomic DNA. Translation: CAA96329.1. BK006947 Genomic DNA. Translation: DAA10589.1.
PIR	S63379.
RefSeq	NP_014446.3. NM_001183225.3.
3D structure databases
ProteinModelPortal	P53740.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1250N.
IntAct	P53740. 3 interactions.
MINT	MINT-402257.
STRING	4932.YNR048W.
Proteomic databases
PaxDb	P53740.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNR048W; YNR048W; YNR048W.
GeneID	855784.
KEGG	sce:YNR048W. sce:YNR055C.
Organism-specific databases
CYGD	YNR048w.
SGD	S000005331. YNR048W.
Phylogenomic databases
eggNOG	COG5035.
GeneTree	ENSGT00390000004660.
HOGENOM	HOG000209056.
OMA	YIADIEL.
OrthoDB	EOG4NCQNQ.
Gene expression databases
Genevestigator	P53740.
GermOnline	YNR048W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005045. DUF284_TM_euk. [Graphical view]
PANTHER	PTHR10926. PTHR10926. 1 hit.
Pfam	PF03381. CDC50. 1 hit. [Graphical view]
PIRSF	PIRSF015840. DUF284_TM_euk. 1 hit.
ProtoNet	Search...
Other
NextBio	980259.

Entry information

Entry name

YN8S_YEAST

Accession

Primary (citable) accession number: P53740
Secondary accession number(s): D6W1M3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program