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P53739

- FPK1_YEAST

UniProt

P53739 - FPK1_YEAST

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Protein
Flippase kinase 1
Gene
FPK1, YNR047W, N3449
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Flippase activator that phosphorylates DFN1 and DFN2 and which is involved in the generation of phospholipid asymmetry in membranes by the inward translocation of phospholipids and in the retrieval pathway from early endosomes to the trans-Golgi network (TGN). Phosphorylates also the N-terminal half of YPK1. Involved in pheromone-response.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Down-regulated by YKP1 phosphorylation. This effect is counteracted in the presence of mannosyl-inositolphosphorylceramide (MIPC).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei525 – 5251ATP By similarity
Active sitei621 – 6211Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi502 – 5109ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein kinase activity Source: SGD
  3. protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  1. lipid transport Source: UniProtKB-KW
  2. positive regulation of phospholipid translocation Source: SGD
  3. protein autophosphorylation Source: SGD
  4. protein phosphorylation Source: SGD
  5. response to pheromone Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33354-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flippase kinase 1 (EC:2.7.11.1)
Gene namesi
Name:FPK1
Ordered Locus Names:YNR047W
ORF Names:N3449
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNR047w.
SGDiS000005330. FPK1.

Subcellular locationi

Cytoplasm. Cell membrane; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 893893Flippase kinase 1
PRO_0000086154Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei140 – 1401Phosphoserine2 Publications
Modified residuei144 – 1441Phosphoserine1 Publication
Modified residuei171 – 1711Phosphoserine1 Publication
Modified residuei175 – 1751Phosphoserine1 Publication
Modified residuei185 – 1851Phosphoserine1 Publication
Modified residuei300 – 3001Phosphoserine2 Publications
Modified residuei414 – 4141Phosphoserine3 Publications
Modified residuei462 – 4621Phosphoserine2 Publications

Post-translational modificationi

The N-terminal non-catalytic domain is phosphorylated by YPK1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53739.
PaxDbiP53739.
PRIDEiP53739.

Expressioni

Gene expression databases

GenevestigatoriP53739.

Interactioni

Protein-protein interaction databases

BioGridi35872. 80 interactions.
DIPiDIP-6427N.
IntActiP53739. 6 interactions.
MINTiMINT-601192.
STRINGi4932.YNR047W.

Structurei

3D structure databases

ProteinModelPortaliP53739.
SMRiP53739. Positions 420-854.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini496 – 777282Protein kinase
Add
BLAST
Domaini778 – 86184AGC-kinase C-terminal
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00530000063286.
KOiK08286.
OMAiGKIAYTP.
OrthoDBiEOG7R2BT5.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR011993. PH_like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53739-1 [UniParc]FASTAAdd to Basket

« Hide

MAGHHHEHEQ ERDHEQEHEH DSLQRPTTGS ERTRSISFSK LLTRSWKRNA    50
SSSNNMSVSS VNLYSDPENS RESDHNNSGS EGQSSRFSKL KSMFQSGNSS 100
KNASAHNSSQ SSLEGDSASS SSKLRYVKPM TSVANASPAS PPLSPTIPET 150
DVLQTPKMVH IDQHEHEREH SNCGSPIMLS SSSFSPTVAR TGTGRRRSPS 200
TPIMPSQNSN NSSSTSAIRP NNYRHHSGSQ GFSSNNPFRE RAGTVRSSNP 250
YFAYQGLPTH AMSSHDLDEG FQPYANGSGI HFLSTPTSKT NSLTNTKNLS 300
NLSLNEIKEN EEVQEFNNED FFFHDIPKDL SLKDTLNGSP SRGSSKSPTI 350
TQTFPSIIVG FDNEYEEDNN NDKHDEKEEQ QTTTDNKTRN LSPTKQNGKA 400
THPRIKIPLR RAASEPNGLQ LASATSPTSS SARKTSGSSN INDKIPGQSV 450
PPPNSFFPQE PSPKISDFPE PRRSRRLRTK SFSNKFQDIM VGPQSFEKIR 500
LLGQGDVGKV FLVREKKTNR VYALKVLSKD EMIKRNKIKR VLTEQEILAT 550
SNHPFIVTLY HSFQSEDYLY LCMEYCMGGE FFRALQTRKT KCICEDDARF 600
YASEVTAALE YLHLLGFIYR DLKPENILLH QSGHIMLSDF DLSIQAKDSK 650
VPVVKGSAQS TLVDTKICSD GFRTNSFVGT EEYIAPEVIR GNGHTAAVDW 700
WTLGILIYEM LFGFTPFKGD NTNETFTNIL KNEVSFPNNN EISRTCKDLI 750
KKLLTKNESK RLGCKMGAAD VKKHPFFKKV QWSLLRNQEP PLIPVLSEDG 800
YDFAKLSSNK KRQTSQDSHK HLDEQEKNMF EERVEYDDEV SEDDPFHDFN 850
SMSLMEQDNN SMIYGNTNSY GKIAYTPNSN RSRSNSHRTF FKR 893
Length:893
Mass (Da):100,546
Last modified:October 1, 1996 - v1
Checksum:i26AF74EE956F80DB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z71662 Genomic DNA. Translation: CAA96328.1.
BK006947 Genomic DNA. Translation: DAA10588.1.
PIRiS63378.
RefSeqiNP_014445.1. NM_001183224.1.

Genome annotation databases

EnsemblFungiiYNR047W; YNR047W; YNR047W.
GeneIDi855783.
KEGGisce:YNR047W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z71662 Genomic DNA. Translation: CAA96328.1 .
BK006947 Genomic DNA. Translation: DAA10588.1 .
PIRi S63378.
RefSeqi NP_014445.1. NM_001183224.1.

3D structure databases

ProteinModelPortali P53739.
SMRi P53739. Positions 420-854.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35872. 80 interactions.
DIPi DIP-6427N.
IntActi P53739. 6 interactions.
MINTi MINT-601192.
STRINGi 4932.YNR047W.

Proteomic databases

MaxQBi P53739.
PaxDbi P53739.
PRIDEi P53739.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNR047W ; YNR047W ; YNR047W .
GeneIDi 855783.
KEGGi sce:YNR047W.

Organism-specific databases

CYGDi YNR047w.
SGDi S000005330. FPK1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00530000063286.
KOi K08286.
OMAi GKIAYTP.
OrthoDBi EOG7R2BT5.

Enzyme and pathway databases

BioCyci YEAST:G3O-33354-MONOMER.

Miscellaneous databases

NextBioi 980256.
PROi P53739.

Gene expression databases

Genevestigatori P53739.

Family and domain databases

Gene3Di 2.30.29.30. 1 hit.
InterProi IPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR011993. PH_like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Identification of novel pheromone-response regulators through systematic overexpression of 120 protein kinases in yeast."
    Burchett S.A., Scott A., Errede B., Dohlman H.G.
    J. Biol. Chem. 276:26472-26478(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-414, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "Protein kinases Fpk1p and Fpk2p are novel regulators of phospholipid asymmetry."
    Nakano K., Yamamoto T., Kishimoto T., Noji T., Tanaka K.
    Mol. Biol. Cell 19:1783-1797(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-140; SER-185; SER-300; SER-414 AND SER-462, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-140; SER-144; SER-171; SER-175; SER-300; SER-414 AND SER-462, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "A protein kinase network regulates the function of aminophospholipid flippases."
    Roelants F.M., Baltz A.G., Trott A.E., Fereres S., Thorner J.
    Proc. Natl. Acad. Sci. U.S.A. 107:34-39(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION BY YPK1, ENZYME REGULATION.

Entry informationi

Entry nameiFPK1_YEAST
AccessioniPrimary (citable) accession number: P53739
Secondary accession number(s): D6W1M2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 752 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

Similar proteinsi