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Protein

Flippase kinase 1

Gene

FPK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Flippase activator that phosphorylates DNF1 and DNF2 and which is involved in the generation of phospholipid asymmetry in membranes by the inward translocation of phospholipids and in the retrieval pathway from early endosomes to the trans-Golgi network (TGN). Phosphorylates also the N-terminal half of YPK1. Involved in pheromone-response.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Down-regulated by YKP1 phosphorylation. This effect is counteracted in the presence of mannosyl-inositolphosphorylceramide (MIPC).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei525ATPPROSITE-ProRule annotation1
Active sitei621Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi502 – 510ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: SGD
  • protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  • intracellular signal transduction Source: GO_Central
  • lipid transport Source: UniProtKB-KW
  • positive regulation of phospholipid translocation Source: SGD
  • protein autophosphorylation Source: SGD
  • protein phosphorylation Source: SGD
  • response to pheromone involved in conjugation with cellular fusion Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33354-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flippase kinase 1 (EC:2.7.11.1)
Gene namesi
Name:FPK1
Ordered Locus Names:YNR047W
ORF Names:N3449
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNR047W.
SGDiS000005330. FPK1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • mating projection tip Source: SGD
  • nucleus Source: GO_Central
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000861541 – 893Flippase kinase 1Add BLAST893

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei140PhosphoserineCombined sources1
Modified residuei144PhosphoserineCombined sources1
Modified residuei171PhosphoserineCombined sources1
Modified residuei175PhosphoserineCombined sources1
Modified residuei185PhosphoserineCombined sources1
Modified residuei300PhosphoserineCombined sources1
Modified residuei414PhosphoserineCombined sources1
Modified residuei462PhosphoserineCombined sources1

Post-translational modificationi

The N-terminal non-catalytic domain is phosphorylated by YPK1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53739.
PRIDEiP53739.

PTM databases

iPTMnetiP53739.

Interactioni

Protein-protein interaction databases

BioGridi35872. 80 interactors.
DIPiDIP-6427N.
IntActiP53739. 6 interactors.
MINTiMINT-601192.

Structurei

3D structure databases

ProteinModelPortaliP53739.
SMRiP53739.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini496 – 777Protein kinasePROSITE-ProRule annotationAdd BLAST282
Domaini778 – 861AGC-kinase C-terminalAdd BLAST84

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. KIN82 subfamily.PROSITE-ProRule annotation
Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000063286.
InParanoidiP53739.
KOiK08286.
OMAiGKIAYTP.
OrthoDBiEOG092C0HH3.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53739-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGHHHEHEQ ERDHEQEHEH DSLQRPTTGS ERTRSISFSK LLTRSWKRNA
60 70 80 90 100
SSSNNMSVSS VNLYSDPENS RESDHNNSGS EGQSSRFSKL KSMFQSGNSS
110 120 130 140 150
KNASAHNSSQ SSLEGDSASS SSKLRYVKPM TSVANASPAS PPLSPTIPET
160 170 180 190 200
DVLQTPKMVH IDQHEHEREH SNCGSPIMLS SSSFSPTVAR TGTGRRRSPS
210 220 230 240 250
TPIMPSQNSN NSSSTSAIRP NNYRHHSGSQ GFSSNNPFRE RAGTVRSSNP
260 270 280 290 300
YFAYQGLPTH AMSSHDLDEG FQPYANGSGI HFLSTPTSKT NSLTNTKNLS
310 320 330 340 350
NLSLNEIKEN EEVQEFNNED FFFHDIPKDL SLKDTLNGSP SRGSSKSPTI
360 370 380 390 400
TQTFPSIIVG FDNEYEEDNN NDKHDEKEEQ QTTTDNKTRN LSPTKQNGKA
410 420 430 440 450
THPRIKIPLR RAASEPNGLQ LASATSPTSS SARKTSGSSN INDKIPGQSV
460 470 480 490 500
PPPNSFFPQE PSPKISDFPE PRRSRRLRTK SFSNKFQDIM VGPQSFEKIR
510 520 530 540 550
LLGQGDVGKV FLVREKKTNR VYALKVLSKD EMIKRNKIKR VLTEQEILAT
560 570 580 590 600
SNHPFIVTLY HSFQSEDYLY LCMEYCMGGE FFRALQTRKT KCICEDDARF
610 620 630 640 650
YASEVTAALE YLHLLGFIYR DLKPENILLH QSGHIMLSDF DLSIQAKDSK
660 670 680 690 700
VPVVKGSAQS TLVDTKICSD GFRTNSFVGT EEYIAPEVIR GNGHTAAVDW
710 720 730 740 750
WTLGILIYEM LFGFTPFKGD NTNETFTNIL KNEVSFPNNN EISRTCKDLI
760 770 780 790 800
KKLLTKNESK RLGCKMGAAD VKKHPFFKKV QWSLLRNQEP PLIPVLSEDG
810 820 830 840 850
YDFAKLSSNK KRQTSQDSHK HLDEQEKNMF EERVEYDDEV SEDDPFHDFN
860 870 880 890
SMSLMEQDNN SMIYGNTNSY GKIAYTPNSN RSRSNSHRTF FKR
Length:893
Mass (Da):100,546
Last modified:October 1, 1996 - v1
Checksum:i26AF74EE956F80DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71662 Genomic DNA. Translation: CAA96328.1.
BK006947 Genomic DNA. Translation: DAA10588.1.
PIRiS63378.
RefSeqiNP_014445.1. NM_001183224.1.

Genome annotation databases

EnsemblFungiiYNR047W; YNR047W; YNR047W.
GeneIDi855783.
KEGGisce:YNR047W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71662 Genomic DNA. Translation: CAA96328.1.
BK006947 Genomic DNA. Translation: DAA10588.1.
PIRiS63378.
RefSeqiNP_014445.1. NM_001183224.1.

3D structure databases

ProteinModelPortaliP53739.
SMRiP53739.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35872. 80 interactors.
DIPiDIP-6427N.
IntActiP53739. 6 interactors.
MINTiMINT-601192.

PTM databases

iPTMnetiP53739.

Proteomic databases

MaxQBiP53739.
PRIDEiP53739.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNR047W; YNR047W; YNR047W.
GeneIDi855783.
KEGGisce:YNR047W.

Organism-specific databases

EuPathDBiFungiDB:YNR047W.
SGDiS000005330. FPK1.

Phylogenomic databases

GeneTreeiENSGT00530000063286.
InParanoidiP53739.
KOiK08286.
OMAiGKIAYTP.
OrthoDBiEOG092C0HH3.

Enzyme and pathway databases

BioCyciYEAST:G3O-33354-MONOMER.

Miscellaneous databases

PROiP53739.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFPK1_YEAST
AccessioniPrimary (citable) accession number: P53739
Secondary accession number(s): D6W1M2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 752 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.