Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Multifunctional methyltransferase subunit TRM112

Gene

TRM112

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as an activator of both rRNA/tRNA and protein methyltransferases. Together with methyltransferase MTQ2, required for the methylation of eRF1 on 'Gln-182'. Together with methyltransferase TRM11, required for the formation of 2-methylguanosine at position 10 (m2G10) in tRNA. Together with methyltransferase BUD23, required for the formation of 7-methylguanine at position 1575 (m7G1575) in 18S rRNA. Involved in biogenesis of both 40S and 60S ribosomal subunits.4 Publications

Miscellaneous

Present with 4800 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • maturation of LSU-rRNA Source: SGD
  • maturation of SSU-rRNA Source: SGD
  • peptidyl-glutamine methylation Source: SGD
  • ribosomal large subunit biogenesis Source: SGD
  • ribosomal small subunit biogenesis Source: SGD
  • rRNA (guanine-N7)-methylation Source: SGD
  • tRNA methylation Source: SGD
  • tRNA modification Source: Reactome

Enzyme and pathway databases

BioCyciMetaCyc:G3O-33353-MONOMER
YEAST:G3O-33353-MONOMER
ReactomeiR-SCE-156581 Methylation
R-SCE-72764 Eukaryotic Translation Termination

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional methyltransferase subunit TRM112
Alternative name(s):
eRF1 methyltransferase subunit TRM112
Short name:
eRF1 MTase subunit TRM112
tRNA methyltransferase 112
Gene namesi
Name:TRM112
Ordered Locus Names:YNR046W
ORF Names:N3445
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNR046W
SGDiS000005329 TRM112

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002158051 – 135Multifunctional methyltransferase subunit TRM112Add BLAST135

Proteomic databases

MaxQBiP53738
PaxDbiP53738
PRIDEiP53738

Interactioni

Subunit structurei

Heterodimer of MTQ2-TRM112, forming the eRF1 methyltransferase. TRM112 is necessary for the solubility and activity of the catalytic subunit MTQ2. Interacts with TRM11; required for full tRNA methyltransferase activity. Interacts with BUD23; required for full rRNA methyltransferase activity. Interacts with RCM1, NOP2, TRM9 and LYS9.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35871, 79 interactors
DIPiDIP-2052N
IntActiP53738, 8 interactors
MINTiP53738
STRINGi4932.YNR046W

Structurei

Secondary structure

1135
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 7Combined sources5
Helixi14 – 16Combined sources3
Beta strandi22 – 24Combined sources3
Turni29 – 31Combined sources3
Beta strandi33 – 36Combined sources4
Helixi44 – 53Combined sources10
Helixi56 – 65Combined sources10
Helixi82 – 84Combined sources3
Helixi87 – 100Combined sources14
Beta strandi103 – 111Combined sources9
Turni113 – 115Combined sources3
Beta strandi118 – 122Combined sources5
Beta strandi125 – 128Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J6AX-ray1.70A1-135[»]
4QTTX-ray2.00A/C1-135[»]
4QTUX-ray2.12A/C1-135[»]
ProteinModelPortaliP53738
SMRiP53738
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53738

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 131TRM112Add BLAST130

Sequence similaritiesi

Belongs to the TRM112 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000009268
HOGENOMiHOG000181652
InParanoidiP53738
KOiK15448
OMAiEEGAMVC
OrthoDBiEOG092C5OO3

Family and domain databases

InterProiView protein in InterPro
IPR005651 Trm112-like
PfamiView protein in Pfam
PF03966 Trm112p, 1 hit

Sequencei

Sequence statusi: Complete.

P53738-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFLTTNFLK CSVKACDTSN DNFPLQYDGS KCQLVQDESI EFNPEFLLNI
60 70 80 90 100
VDRVDWPAVL TVAAELGNNA LPPTKPSFPS SIQELTDDDM AILNDLHTLL
110 120 130
LQTSIAEGEM KCRNCGHIYY IKNGIPNLLL PPHLV
Length:135
Mass (Da):15,066
Last modified:October 1, 1996 - v1
Checksum:i3CFB2ABDDFB0E38A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71661 Genomic DNA Translation: CAA96327.1
BK006947 Genomic DNA Translation: DAA10587.1
PIRiS63377
RefSeqiNP_014444.1, NM_001183223.1

Genome annotation databases

EnsemblFungiiYNR046W; YNR046W; YNR046W
GeneIDi855782
KEGGisce:YNR046W

Similar proteinsi

Entry informationi

Entry nameiTR112_YEAST
AccessioniPrimary (citable) accession number: P53738
Secondary accession number(s): D6W1M1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health