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Protein

ATP-dependent RNA helicase DBP6

Gene

DBP6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits and is required for the normal formation of 25S and 5.8S rRNAs.4 Publications

Miscellaneous

Present with 12700 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi234 – 241ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: SGD
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • RNA secondary structure unwinding Source: GO_Central

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processRibosome biogenesis, rRNA processing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33348-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DBP6 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 6
Gene namesi
Name:DBP6
Ordered Locus Names:YNR038W
ORF Names:N3302
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi

Organism-specific databases

EuPathDBiFungiDB:YNR038W
SGDiS000005321 DBP6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550361 – 629ATP-dependent RNA helicase DBP6Add BLAST629

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei73PhosphoserineCombined sources1
Modified residuei77PhosphoserineCombined sources1
Modified residuei78PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53734
PaxDbiP53734
PRIDEiP53734

PTM databases

iPTMnetiP53734

Interactioni

Subunit structurei

Associated with pre-ribosomal particles. Interacts with DBP9 and RSA3. Together with NOP8, URB1, URB2 and RSA3, forms an RNA-independent complex, which is required during early maturation of nascent 60S ribosomal subunits.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35864, 256 interactors
IntActiP53734, 43 interactors
MINTiP53734
STRINGi4932.YNR038W

Structurei

3D structure databases

ProteinModelPortaliP53734
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini221 – 401Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST181
Domaini437 – 603Helicase C-terminalPROSITE-ProRule annotationAdd BLAST167

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi197 – 205Q motif9
Motifi341 – 344DEAD box4

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000075141
HOGENOMiHOG000203294
InParanoidiP53734
KOiK14807
OMAiSFQGWLD
OrthoDBiEOG092C1FFB

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

P53734-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFASRFDPSQ LTAPAASAPE GIVGTTPPAI VPLKRQATES DNEEYGSHQD
60 70 80 90 100
SDESSNSSSE EDEDRMQVDY GASEEDSSEV EEEESKPSTH STVLSRFKQT
110 120 130 140 150
VSLQERLGAS DIAESKEDEG IEDEAASTHQ LKQIPQPEFV KNPMNLNTNS
160 170 180 190 200
LQFKSTGWLN TEKIYYDNSL IKPFSDYANE LEAKLLQNIC KNFSTNTFPI
210 220 230 240 250
QSIILDSILP VLNFTLNVSK RNFTRRIGDI LVNAATGSGK TLAYSIPIVQ
260 270 280 290 300
TLFKRQINRL RCIIIVPTKL LINQVYTTLT KLTQGTSLIV SIAKLENSLK
310 320 330 340 350
DEHKKLSNLE PDILITTPGR LVDHLNMKSI NLKNLKFLII DEADRLLNQS
360 370 380 390 400
FQGWCPKLMS HLKTDKLDTL PGNVIKMIFS ATLTTNTEKL NGLNLYKPKL
410 420 430 440 450
FLKQTDKLYQ LPNKLNEFNI NIPTAKSVYK PLILLYSICQ FMAHSPIAAK
460 470 480 490 500
ILIFVKSNES SIRLSKLLQL ICESRSQSSV LKNLQNLAVS INSVNSNNSK
510 520 530 540 550
AENKKIVANF SHHSESAGIT ILITTDIMSR GIDINDITQV INYDPPMSSQ
560 570 580 590 600
QYVHRVGRTA RANELGSAYN LLVGRGERTF FDDLNKDLDR DGKSVQPLEL
610 620
DFTLLESDSE LYTSSLESLK NYHNNTAQA
Length:629
Mass (Da):70,362
Last modified:October 1, 1996 - v1
Checksum:i64A04A2D88F44072
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71653 Genomic DNA Translation: CAA96318.1
BK006947 Genomic DNA Translation: DAA10580.1
PIRiS63369
RefSeqiNP_014436.1, NM_001183215.1

Genome annotation databases

EnsemblFungiiYNR038W; YNR038W; YNR038W
GeneIDi855774
KEGGisce:YNR038W

Entry informationi

Entry nameiDBP6_YEAST
AccessioniPrimary (citable) accession number: P53734
Secondary accession number(s): D6W1L4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

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