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Protein

Actin-related protein 2/3 complex subunit 2

Gene

ARC35

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament (By similarity).By similarity

GO - Molecular functioni

  • structural molecule activity Source: SGD

GO - Biological processi

  • actin cytoskeleton organization Source: SGD
  • Arp2/3 complex-mediated actin nucleation Source: InterPro
  • microtubule cytoskeleton organization Source: SGD
  • mitochondrion inheritance Source: SGD
  • receptor-mediated endocytosis Source: SGD
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33345-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-related protein 2/3 complex subunit 2
Alternative name(s):
Arp2/3 complex 34 kDa subunit
Short name:
p34-ARC
Gene namesi
Name:ARC35
Ordered Locus Names:YNR035C
ORF Names:N3296
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNR035C.
SGDiS000005318. ARC35.

Subcellular locationi

GO - Cellular componenti

  • actin cortical patch Source: UniProtKB-SubCell
  • Arp2/3 protein complex Source: SGD
  • cytosol Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342Actin-related protein 2/3 complex subunit 2PRO_0000124041Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei155 – 1551PhosphothreonineCombined sources
Modified residuei324 – 3241PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53731.
PeptideAtlasiP53731.

PTM databases

iPTMnetiP53731.

Interactioni

Subunit structurei

Component of the Arp2/3 complex composed of ARP2, ARP3, ARC40/p41-ARC, ARC35/p34-ARC, ARC18/p21-ARC, ARC19/p20-ARC and ARC16/p16-ARC.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ARC18Q059335EBI-2770,EBI-2764
ARC19P332043EBI-2770,EBI-2757
ARC40P383285EBI-2770,EBI-2777
ARP2P323815EBI-2770,EBI-2927

Protein-protein interaction databases

BioGridi35861. 124 interactions.
DIPiDIP-2304N.
IntActiP53731. 15 interactions.
MINTiMINT-498308.

Structurei

3D structure databases

ProteinModelPortaliP53731.
SMRiP53731. Positions 1-315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ARPC2 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000016794.
HOGENOMiHOG000212535.
InParanoidiP53731.
KOiK05758.
OMAiDNCISHI.
OrthoDBiEOG7380FP.

Family and domain databases

InterProiIPR007188. ARPC2.
[Graphical view]
PANTHERiPTHR12058. PTHR12058. 2 hits.
PfamiPF04045. P34-Arc. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53731-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLHLQPQNLL IQKTLNEAIE ALRKGSPLTM DRIVSDFDYT TYHISNTAED
60 70 80 90 100
KSILLLSVKT KAWVSVSECQ LDGSLTLLKF LADHYSSLGG VTIPSEVEPG
110 120 130 140 150
YDYTLQITLA ELVQESILQL SVLKTIILSF PFELAISKFI ELSQQQPAPV
160 170 180 190 200
EAEITGGEVA ANGDNTLFTI KYRDEENIFI KPSNDRVTII FETIFQDETD
210 220 230 240 250
KIFGKVFLQE FVDARKRNRQ IQSAPQVLYS HEPPLELKRL YQPPKVAEQS
260 270 280 290 300
RRFITFVLFP RHFQTKELQF HSICQLTLFR NYFHYHIKCS KAYMHSRMRF
310 320 330 340
RVDSFIKVLN RAKVDEDDEN DELSAEGRQQ ARRTFTGRKI VY
Length:342
Mass (Da):39,566
Last modified:October 1, 1996 - v1
Checksum:i9AF71A93688729F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71650 Genomic DNA. Translation: CAA96315.1.
BK006947 Genomic DNA. Translation: DAA10577.1.
PIRiS63366.
RefSeqiNP_014433.1. NM_001183212.1.

Genome annotation databases

EnsemblFungiiYNR035C; YNR035C; YNR035C.
GeneIDi855771.
KEGGisce:YNR035C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71650 Genomic DNA. Translation: CAA96315.1.
BK006947 Genomic DNA. Translation: DAA10577.1.
PIRiS63366.
RefSeqiNP_014433.1. NM_001183212.1.

3D structure databases

ProteinModelPortaliP53731.
SMRiP53731. Positions 1-315.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35861. 124 interactions.
DIPiDIP-2304N.
IntActiP53731. 15 interactions.
MINTiMINT-498308.

PTM databases

iPTMnetiP53731.

Proteomic databases

MaxQBiP53731.
PeptideAtlasiP53731.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNR035C; YNR035C; YNR035C.
GeneIDi855771.
KEGGisce:YNR035C.

Organism-specific databases

EuPathDBiFungiDB:YNR035C.
SGDiS000005318. ARC35.

Phylogenomic databases

GeneTreeiENSGT00390000016794.
HOGENOMiHOG000212535.
InParanoidiP53731.
KOiK05758.
OMAiDNCISHI.
OrthoDBiEOG7380FP.

Enzyme and pathway databases

BioCyciYEAST:G3O-33345-MONOMER.

Miscellaneous databases

NextBioi980223.
PROiP53731.

Family and domain databases

InterProiIPR007188. ARPC2.
[Graphical view]
PANTHERiPTHR12058. PTHR12058. 2 hits.
PfamiPF04045. P34-Arc. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches."
    Winter D., Podtelejnikov A.V., Mann M., Li R.
    Curr. Biol. 7:519-529(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE ARP2/3 COMPLEX.
  4. Erratum
    Winter D., Podtelejnikov A.V., Mann M., Li R.
    Curr. Biol. 7:R593-R593(1997)
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-155 AND SER-324, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiARPC2_YEAST
AccessioniPrimary (citable) accession number: P53731
Secondary accession number(s): D6W1L1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 13400 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.