Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P53730 (ALG12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 101. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
EC=2.4.1.260

Alternative name(s):
Asparagine-linked glycosylation protein 12
Dolichyl-P-Man:Man(7)GlcNAc(2)-PP-dolichyl-alpha-1,6-mannosyltransferase
Extracellular mutant protein 39
Mannosyltransferase ALG12

Gene names

Name:	ALG12
Synonyms:	ECM39
Ordered Locus Names:	YNR030W
ORF Names:	N3265

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	551 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adds the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor (dolichol-PP-Man₇GlcNAc₂) required for protein glycosylation. Ref.2 Ref.5
Catalytic activity	Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate. Ref.2
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.6.
Miscellaneous	Present with 3550 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the glycosyltransferase 22 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GPI anchor biosynthetic process Inferred from electronic annotation. Source: InterPro dolichol-linked oligosaccharide biosynthetic process Inferred from mutant phenotype Ref.5. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro
Molecular function	alpha-1,6-mannosyltransferase activity Inferred from mutant phenotype Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
KAR2	P16474	1	EBI-28496,EBI-7876

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 551

551

Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase

PRO_0000215786

Regions

Topological domain

1 – 2

Lumenal Potential

Transmembrane

3 – 23

Helical; Potential

Topological domain

24 – 61

Cytoplasmic Potential

Transmembrane

62 – 82

Helical; Potential

Topological domain

83 – 89

Lumenal Potential

Transmembrane

90 – 110

Helical; Potential

Topological domain

111 – 136

Cytoplasmic Potential

Transmembrane

137 – 157

Helical; Potential

Topological domain

158 – 178

Lumenal Potential

Transmembrane

179 – 199

Helical; Potential

Topological domain

200 – 202

Cytoplasmic Potential

Transmembrane

203 – 223

Helical; Potential

Topological domain

224 – 227

Lumenal Potential

Transmembrane

228 – 248

Helical; Potential

Topological domain

249 – 275

Cytoplasmic Potential

Transmembrane

276 – 296

Helical; Potential

Topological domain

297 – 303

Lumenal Potential

Transmembrane

304 – 324

Helical; Potential

Topological domain

325 – 331

Cytoplasmic Potential

Transmembrane

332 – 352

Helical; Potential

Topological domain

353 – 365

Lumenal Potential

Transmembrane

366 – 386

Helical; Potential

Topological domain

387 – 417

Cytoplasmic Potential

Transmembrane

418 – 438

Helical; Potential

Topological domain

439 – 551

113

Lumenal Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P53730 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: B08A94BBF260502F
Show »

FASTA

551

62,672

        10         20         30         40         50         60 
MRWSVLDTVL LTVISFHLIQ APFTKVEESF NIQAIHDILT YSVFDISQYD HLKFPGVVPR 

        70         80         90        100        110        120 
TFVGAVIIAM LSRPYLYLSS LIQTSRPTSI DVQLVVRGIV GLTNGLSFIY LKNCLQDMFD 

       130        140        150        160        170        180 
EITEKKKEEN EDKDIYIYDS AGTWFLLFLI GSFHLMFYST RTLPNFVMTL PLTNVALGWV 

       190        200        210        220        230        240 
LLGRYNAAIF LSALVAIVFR LEVSALSAGI ALFSVIFKKI SLFDAIKFGI FGLGLGSAIS 

       250        260        270        280        290        300 
ITVDSYFWQE WCLPEVDGFL FNVVAGYASK WGVEPVTAYF THYLRMMFMP PTVLLLNYFG 

       310        320        330        340        350        360 
YKLAPAKLKI VSLASLFHII VLSFQPHKEW RFIIYAVPSI MLLGATGAAH LWENMKVKKI 

       370        380        390        400        410        420 
TNVLCLAILP LSIMTSFFIS MAFLYISRMN YPGGEALTSF NDMIVEKNIT NATVHISIPP 

       430        440        450        460        470        480 
CMTGVTLFGE LNYGVYGINY DKTENTTLLQ EMWPSFDFLI THEPTASQLP FENKTTNHWE 

       490        500        510        520        530        540 
LVNTTKMFTG FDPTYIKNFV FQERVNVLSL LKQIIFDKTP TVFLKELTAN SIVKSDVFFT 

       550 
YKRIKQDEKT D

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis." Frank C.G., Aebi M. Glycobiology 15:1156-1163(2005) [PubMed: 15987956] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Ordered assembly of the asymmetrically branched lipid-linked oligosaccharide in the endoplasmic reticulum is ensured by the substrate specificity of the individual glycosyltransferases." Burda P., Jakob C.A., Beinhauer J., Hegemann J.H., Aebi M. Glycobiology 9:617-625(1999) [PubMed: 10336995] [Abstract] Cited for: FUNCTION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z71645 Genomic DNA. Translation: CAA96310.1. AY692956 Genomic DNA. Translation: AAT92975.1. BK006947 Genomic DNA. Translation: DAA10570.1.
PIR	S63361.
RefSeq	NP_014427.1. NM_001183207.1.
3D structure databases
ProteinModelPortal	P53730.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4259N.
IntAct	P53730. 23 interactions.
MINT	MINT-478020.
STRING	P53730.
Protein family/group databases
CAZy	GT22. Glycosyltransferase Family 22.
Proteomic databases
PeptideAtlas	P53730.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNR030W; YNR030W; YNR030W.
GeneID	855764.
KEGG	sce:YNR030W.
NMPDR	fig\|4932.3.peg.5507.
Organism-specific databases
CYGD	YNR030w.
SGD	S000005313. ALG12.
Phylogenomic databases
eggNOG	fuNOG05751.
GeneTree	EFGT00050000003340.
HOGENOM	HBG397739.
OMA	IQAIHDI.
OrthoDB	EOG483HD7.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-7190.
Gene expression databases
ArrayExpress	P53730.
Genevestigator	P53730.
GermOnline	YNR030W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005599. GPI_mannosylTrfase. [Graphical view]
KO	K03847.
PANTHER	PTHR22760. Alg9_trans. 1 hit.
Pfam	PF03901. Glyco_transf_22. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980202.

Entry information

Entry name

ALG12_YEAST

Accession

Primary (citable) accession number: P53730
Secondary accession number(s): D6W1K4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents