P53728 (CYP8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptidyl-prolyl cis-trans isomerase CYP8
Short name=PPIase CYP8
EC=5.2.1.8

Alternative name(s):
Rotamase CYP8

Gene names

Name:	CPR8
Ordered Locus Names:	YNR028W
ORF Names:	N3255

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides By similarity.
Catalytic activity	Peptidylproline (omega=180) = peptidylproline (omega=0).
Sequence similarities	Contains 1 PPIase cyclophilin-type domain.

Ontologies

Keywords
Molecular function	Isomerase Rotamase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	fungal-type vacuole Inferred from direct assay. Source: SGD
Molecular function	peptidyl-prolyl cis-trans isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Peptidyl-prolyl cis-trans isomerase CYP8		PRO_0000064175
Regions
	Domain	56 – 215	160	PPIase cyclophilin-type
Experimental info
	Sequence conflict	8	1	L → P in AAT92973. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P53728 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 4E2E75B66134E6B0	FASTA	308	34,946
	10 20 30 40 50 60 MKSFFLYLYV AFMFSCITAL PLPVDNKRAS SDSLDLKKKY APDPPITHNV NIGIVFTDPE 70 80 90 100 110 120 SSEEAGRLIT IDLYGTMVPK TVMTFCQYVD SVKDRLASRH SYSPERDFDK ILPNGAIEGS 130 140 150 160 170 180 SVSSSSIEET EMLAPKLPEE NHSLIHDRPG RVSMIKDDKG LKFIIETSET PLEGESVVFG 190 200 210 220 230 240 QVTAGLKDLM DKLANVKTDE NGKPEQPITI GYISSQEHRI QHAKEAHEKY LQRLQDYQNG 250 260 270 280 290 300 DLEKGITLKN YLYQGSQRKL EDAKYNQLHH PLPKIMLGIS VLLLFYVLAK YRKRIFNRSS KIVSIRED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z71643 Genomic DNA. Translation: CAA96308.1. AY692954 Genomic DNA. Translation: AAT92973.1. BK006947 Genomic DNA. Translation: DAA10568.1.
PIR	S63359.
RefSeq	NP_014425.1. NM_001183205.1.
3D structure databases
ProteinModelPortal	P53728.
SMR	P53728. Positions 43-216.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4261N.
IntAct	P53728. 7 interactions.
MINT	MINT-483276.
STRING	P53728.
Proteomic databases
PeptideAtlas	P53728.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNR028W; YNR028W; YNR028W.
GeneID	855762.
KEGG	sce:YNR028W.
NMPDR	fig|4932.3.peg.5505.
Organism-specific databases
CYGD	YNR028w.
SGD	S000005311. CPR8.
Phylogenomic databases
eggNOG	fuNOG10026.
Gene expression databases
ArrayExpress	P53728.
Genevestigator	P53728.
GermOnline	YNR028W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002130. Cyclophilin-like_PPIase_dom. [Graphical view]
Gene3D	G3DSA:2.40.100.10. PPIase_cyclophilin. 1 hit.
KO	K01802.
Pfam	PF00160. Pro_isomerase. 1 hit. [Graphical view]
PRINTS	PR00153. CSAPPISMRASE.
SUPFAM	SSF50891. CSA_PPIase. 1 hit.
PROSITE	PS00170. CSA_PPIASE_1. False negative. PS50072. CSA_PPIASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980196.

Entry information

Entry name

CYP8_YEAST

Accession

Primary (citable) accession number: P53728
Secondary accession number(s): D6W1K2, E9P900

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program