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P53725

- MPP6_YEAST

UniProt

P53725 - MPP6_YEAST

Protein

M-phase phosphoprotein 6 homolog

Gene

MPP6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA) derived from intergenic regions and the ribosomal DNA spacer heterochromatin. Binds RNA.1 Publication

    GO - Molecular functioni

    1. poly(U) RNA binding Source: SGD

    GO - Biological processi

    1. exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    2. nuclear mRNA surveillance of mRNA 3'-end processing Source: SGD
    3. nuclear mRNA surveillance of spliceosomal pre-mRNA splicing Source: SGD
    4. nuclear polyadenylation-dependent CUT catabolic process Source: SGD
    5. nuclear polyadenylation-dependent rRNA catabolic process Source: SGD

    Keywords - Biological processi

    rRNA processing

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33338-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    M-phase phosphoprotein 6 homolog
    Alternative name(s):
    Exosome-associated RNA-binding protein MPP6
    Gene namesi
    Name:MPP6
    Ordered Locus Names:YNR024W
    ORF Names:N3230
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNR024w.
    SGDiS000005307. MPP6.

    Subcellular locationi

    Nucleus 4 Publications
    Note: Colocalizes with the nuclear exosome and ribosomes.

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Lethal in combination with the absence of either RRP6 or its cofactor RRP47. Weak but detectable stabilization of unspliced pre-mRNAs, and stronger stabilization is detected for mRNAs with defects in 3' cleavage and polyadenylation.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 186185M-phase phosphoprotein 6 homologPRO_0000203474Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei42 – 421Phosphoserine1 Publication
    Modified residuei150 – 1501Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP53725.
    PaxDbiP53725.

    Expressioni

    Gene expression databases

    GenevestigatoriP53725.

    Interactioni

    Protein-protein interaction databases

    BioGridi35849. 37 interactions.
    DIPiDIP-4263N.
    IntActiP53725. 1 interaction.
    MINTiMINT-567258.
    STRINGi4932.YNR024W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53725.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    KOiK12594.
    OMAiMSANNGV.
    OrthoDBiEOG7V76K9.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P53725-1 [UniParc]FASTAAdd to Basket

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    MSANNGVTGK LSSRVMNMKF MKFGKTDDEE SSNSNTPSNI NSDVEPIEQK    50
    GKLFGLDDSA WDLNSYKDDL KKISGKEKKK VKRVVYKKRP NLIISNVGYS 100
    ELRKPEGVIS GRKTFGDNSD DSGSRKRKFD EGEQNEDEKR DAKDKEFTGS 150
    QDDGEDEYDL DKLFKDSIKK KKTNHNGKNK NRNSKK 186
    Length:186
    Mass (Da):21,142
    Last modified:October 1, 1996 - v1
    Checksum:iB1DEE530E6F9379C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71639 Genomic DNA. Translation: CAA96303.1.
    BK006947 Genomic DNA. Translation: DAA10565.1.
    PIRiS63355.
    RefSeqiNP_014421.3. NM_001183201.3.

    Genome annotation databases

    EnsemblFungiiYNR024W; YNR024W; YNR024W.
    GeneIDi855758.
    KEGGisce:YNR024W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71639 Genomic DNA. Translation: CAA96303.1 .
    BK006947 Genomic DNA. Translation: DAA10565.1 .
    PIRi S63355.
    RefSeqi NP_014421.3. NM_001183201.3.

    3D structure databases

    ProteinModelPortali P53725.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35849. 37 interactions.
    DIPi DIP-4263N.
    IntActi P53725. 1 interaction.
    MINTi MINT-567258.
    STRINGi 4932.YNR024W.

    Proteomic databases

    MaxQBi P53725.
    PaxDbi P53725.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNR024W ; YNR024W ; YNR024W .
    GeneIDi 855758.
    KEGGi sce:YNR024W.

    Organism-specific databases

    CYGDi YNR024w.
    SGDi S000005307. MPP6.

    Phylogenomic databases

    KOi K12594.
    OMAi MSANNGV.
    OrthoDBi EOG7V76K9.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33338-MONOMER.

    Miscellaneous databases

    NextBioi 980187.

    Gene expression databases

    Genevestigatori P53725.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes."
      Fleischer T.C., Weaver C.M., McAfee K.J., Jennings J.L., Link A.J.
      Genes Dev. 20:1294-1307(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "A yeast exosome cofactor, Mpp6, functions in RNA surveillance and in the degradation of noncoding RNA transcripts."
      Milligan L., Decourty L., Saveanu C., Rappsilber J., Ceulemans H., Jacquier A., Tollervey D.
      Mol. Cell. Biol. 28:5446-5457(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, RNA-BINDING, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, IDENTIFICATION BY MASS SPECTROMETRY.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42 AND SER-150, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Comparative multiplexed mass spectrometric analyses of endogenously expressed yeast nuclear and cytoplasmic exosomes."
      Synowsky S.A., van Wijk M., Raijmakers R., Heck A.J.
      J. Mol. Biol. 385:1300-1313(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMPP6_YEAST
    AccessioniPrimary (citable) accession number: P53725
    Secondary accession number(s): D6W1J9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1350 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3