P53725 (MPP6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
M-phase phosphoprotein 6 homolog

Alternative name(s):
Exosome-associated RNA-binding protein MPP6

Gene names

Name:	MPP6
Ordered Locus Names:	YNR024W
ORF Names:	N3230

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA) derived from intergenic regions and the ribosomal DNA spacer heterochromatin. Binds RNA. Ref.7
Subcellular location	Nucleus. Note: Colocalizes with the nuclear exosome and ribosomes. Ref.3 Ref.5 Ref.7 Ref.9
Disruption phenotype	Lethal in combination with the absence of either RRP6 or its cofactor RRP47. Weak but detectable stabilization of unspliced pre-mRNAs, and stronger stabilization is detected for mRNAs with defects in 3' cleavage and polyadenylation. Ref.7
Miscellaneous	Present with 1350 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Nucleus
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.7. Source: SGD nuclear mRNA surveillance of mRNA 3'-end processing Inferred from genetic interaction Ref.7. Source: SGD nuclear mRNA surveillance of spliceosomal pre-mRNA splicing Inferred from genetic interaction Ref.7. Source: SGD nuclear polyadenylation-dependent CUT catabolic process Inferred from mutant phenotype Ref.7. Source: SGD nuclear polyadenylation-dependent rRNA catabolic process Inferred from genetic interaction Ref.7. Source: SGD
Cellular_component	nuclear exosome (RNase complex) Inferred from direct assay Ref.9. Source: SGD
Molecular_function	poly(U) RNA binding Inferred from direct assay Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 186	186	M-phase phosphoprotein 6 homolog		PRO_0000203474
Amino acid modifications
	Modified residue	42	1	Phosphoserine Ref.6 Ref.8
	Modified residue	148	1	Phosphothreonine Ref.8
	Modified residue	150	1	Phosphoserine Ref.6 Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P53725 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: B1DEE530E6F9379C	FASTA	186	21,142
	10 20 30 40 50 60 MSANNGVTGK LSSRVMNMKF MKFGKTDDEE SSNSNTPSNI NSDVEPIEQK GKLFGLDDSA 70 80 90 100 110 120 WDLNSYKDDL KKISGKEKKK VKRVVYKKRP NLIISNVGYS ELRKPEGVIS GRKTFGDNSD 130 140 150 160 170 180 DSGSRKRKFD EGEQNEDEKR DAKDKEFTGS QDDGEDEYDL DKLFKDSIKK KKTNHNGKNK NRNSKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes." Fleischer T.C., Weaver C.M., McAfee K.J., Jennings J.L., Link A.J. Genes Dev. 20:1294-1307(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42 AND SER-150, MASS SPECTROMETRY.
[7]	"A yeast exosome cofactor, Mpp6, functions in RNA surveillance and in the degradation of noncoding RNA transcripts." Milligan L., Decourty L., Saveanu C., Rappsilber J., Ceulemans H., Jacquier A., Tollervey D. Mol. Cell. Biol. 28:5446-5457(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, RNA-BINDING, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42; THR-148 AND SER-150, MASS SPECTROMETRY.
[9]	"Comparative multiplexed mass spectrometric analyses of endogenously expressed yeast nuclear and cytoplasmic exosomes." Synowsky S.A., van Wijk M., Raijmakers R., Heck A.J. J. Mol. Biol. 385:1300-1313(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z71639 Genomic DNA. Translation: CAA96303.1. BK006947 Genomic DNA. Translation: DAA10565.1.
PIR	S63355.
RefSeq	NP_014421.3. NM_001183201.3.
3D structure databases
ProteinModelPortal	P53725.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4263N.
IntAct	P53725. 1 interaction.
MINT	MINT-567258.
STRING	4932.YNR024W.
Proteomic databases
PaxDb	P53725.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNR024W; YNR024W; YNR024W.
GeneID	855758.
KEGG	sce:YNR024W.
Organism-specific databases
CYGD	YNR024w.
SGD	S000005307. MPP6.
Phylogenomic databases
KO	K12594.
OMA	NMKFMKF.
Enzyme and pathway databases
BioCyc	YEAST:G3O-33338-MONOMER.
Gene expression databases
Genevestigator	P53725.
GermOnline	YNR024W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	980187.

Entry information

Entry name

MPP6_YEAST

Accession

Primary (citable) accession number: P53725
Secondary accession number(s): D6W1J9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program