P53724 (RM50_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
54S ribosomal protein L50, mitochondrial

Gene names

Name:	MRPL50
Ordered Locus Names:	YNR022C
ORF Names:	N3220

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	139 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S). Ref.3
Subcellular location	Mitochondrion Ref.4 Ref.5.

Ontologies

Keywords
Cellular component	Mitochondrion
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial translation Inferred by curator Ref.3. Source: SGD
Cellular_component	mitochondrial large ribosomal subunit Inferred from physical interaction Ref.3. Source: SGD
Molecular_function	structural constituent of ribosome Inferred from physical interaction Ref.3. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 139	139	54S ribosomal protein L50, mitochondrial		PRO_0000030584

Sequences

	Sequence		Length	Mass (Da)
	P53724 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 9B03AA8C283063E9	FASTA	139	16,283
	10 20 30 40 50 60 MLHCTQVCLS ALTKRTHRVK VQVLKDFPRF QLYKGQVANV KPSLMRNYLH NFNGAKYILS 70 80 90 100 110 120 EEHDINTELL KQYQTLEAKL EEDHQQLSKR HETEVQKNME LRKESVFGHK KEEKPKEEKK 130 GLLDSGITIE EVKIPGLDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components." Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K. Eur. J. Biochem. 269:5203-5214(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, MASS SPECTROMETRY.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics." Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A. J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z71637 Genomic DNA. Translation: CAA96301.1. BK006947 Genomic DNA. Translation: DAA10563.1.
PIR	S63353.
RefSeq	NP_014419.1. NM_001183199.1.
3D structure databases
ProteinModelPortal	P53724.
SMR	P53724. Positions 19-62.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1265N.
IntAct	P53724. 4 interactions.
MINT	MINT-402743.
STRING	4932.YNR022C.
Proteomic databases
PaxDb	P53724.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNR022C; YNR022C; YNR022C.
GeneID	855756.
KEGG	sce:YNR022C.
Organism-specific databases
CYGD	YNR022c.
SGD	S000005305. MRPL50.
Phylogenomic databases
eggNOG	NOG43083.
HOGENOM	HOG000057082.
OMA	RTKRVQV.
OrthoDB	EOG4CVKHS.
Gene expression databases
Genevestigator	P53724.
GermOnline	YNR022C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	980181.

Entry information

Entry name

RM50_YEAST

Accession

Primary (citable) accession number: P53724
Secondary accession number(s): D6W1J7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program