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Protein

Respiratory supercomplex factor 2, mitochondrial

Gene

RCF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cytochrome c oxidase subunit which plays a role in assembly of respiratory supercomplexes. May be required for late-stage assembly of the COX12 and COX13 subunits and for cytochrome c oxidase activity.2 Publications

GO - Biological processi

  • mitochondrial respiratory chain complex IV assembly Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-33333-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Respiratory supercomplex factor 2, mitochondrial
Alternative name(s):
Altered inheritance of mitochondria protein 38
Gene namesi
Name:RCF2
Synonyms:AIM38
Ordered Locus Names:YNR018W
ORF Names:N3185
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNR018W.
SGDiS000005301. RCF2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei19 – 3921HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei50 – 7021HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei152 – 17221HelicalPROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of mitochondrial inner membrane Source: SGD
  • mitochondrial respiratory chain supercomplex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Increases frequency of mitochondrial genome loss.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 224224Respiratory supercomplex factor 2, mitochondrialPRO_0000215780Add
BLAST

Proteomic databases

MaxQBiP53721.

Interactioni

Subunit structurei

Associates with the respiratory chain complex III/complex IV supercomplex.

Protein-protein interaction databases

BioGridi35843. 44 interactions.
DIPiDIP-2726N.
IntActiP53721. 2 interactions.
MINTiMINT-1630572.

Structurei

3D structure databases

ProteinModelPortaliP53721.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini89 – 18092HIG1PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi196 – 1994Poly-Glu

Sequence similaritiesi

Contains 1 HIG1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000248887.
InParanoidiP53721.
OMAiDESTWTG.
OrthoDBiEOG7J9W1R.

Family and domain databases

InterProiIPR007667. Hypoxia_induced_domain.
[Graphical view]
PfamiPF04588. HIG_1_N. 1 hit.
[Graphical view]
PROSITEiPS51503. HIG1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53721-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKILTQDEIE AHRSHTLKGG IEGALAGFAI SAIIFKVLPR RYPKFKPSTL
60 70 80 90 100
TWSIKTALWI TPPTVLTAIC AEEASNNFDA TMYGSGSSSE DALDEHRRWK
110 120 130 140 150
SLSTKDKFVE GLSNNKYKII TGAWAASLYG SWVIVNKDPI MTKAQKIVQA
160 170 180 190 200
RMYAQFITVG LLLASVGLSM YENKLHPNKQ KVNEMRRWEN ALRVAEEEER
210 220
LEKEGRRTGY VSNEERINSK IFKS
Length:224
Mass (Da):25,344
Last modified:October 1, 1996 - v1
Checksum:iFA2C528A008CFE7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71633 Genomic DNA. Translation: CAA96297.1.
BK006947 Genomic DNA. Translation: DAA10559.1.
PIRiS63349.
RefSeqiNP_014415.1. NM_001183195.1.

Genome annotation databases

EnsemblFungiiYNR018W; YNR018W; YNR018W.
GeneIDi855752.
KEGGisce:YNR018W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71633 Genomic DNA. Translation: CAA96297.1.
BK006947 Genomic DNA. Translation: DAA10559.1.
PIRiS63349.
RefSeqiNP_014415.1. NM_001183195.1.

3D structure databases

ProteinModelPortaliP53721.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35843. 44 interactions.
DIPiDIP-2726N.
IntActiP53721. 2 interactions.
MINTiMINT-1630572.

Proteomic databases

MaxQBiP53721.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNR018W; YNR018W; YNR018W.
GeneIDi855752.
KEGGisce:YNR018W.

Organism-specific databases

EuPathDBiFungiDB:YNR018W.
SGDiS000005301. RCF2.

Phylogenomic databases

HOGENOMiHOG000248887.
InParanoidiP53721.
OMAiDESTWTG.
OrthoDBiEOG7J9W1R.

Enzyme and pathway databases

BioCyciYEAST:G3O-33333-MONOMER.

Miscellaneous databases

NextBioi980169.
PROiP53721.

Family and domain databases

InterProiIPR007667. Hypoxia_induced_domain.
[Graphical view]
PfamiPF04588. HIG_1_N. 1 hit.
[Graphical view]
PROSITEiPS51503. HIG1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  6. "Computationally driven, quantitative experiments discover genes required for mitochondrial biogenesis."
    Hess D.C., Myers C.L., Huttenhower C., Hibbs M.A., Hayes A.P., Paw J., Clore J.J., Mendoza R.M., Luis B.S., Nislow C., Giaever G., Costanzo M., Troyanskaya O.G., Caudy A.A.
    PLoS Genet. 5:E1000407-E1000407(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  7. "A three-way proteomics strategy allows differential analysis of yeast mitochondrial membrane protein complexes under anaerobic and aerobic conditions."
    Helbig A.O., de Groot M.J., van Gestel R.A., Mohammed S., de Hulster E.A., Luttik M.A., Daran-Lapujade P., Pronk J.T., Heck A.J., Slijper M.
    Proteomics 9:4787-4798(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ASSOCIATION WITH THE RESPIRATORY CHAIN COMPLEX IV.
  8. "Rcf1 mediates cytochrome oxidase assembly and respirasome formation, revealing heterogeneity of the enzyme complex."
    Vukotic M., Oeljeklaus S., Wiese S., Vogtle F.N., Meisinger C., Meyer H.E., Zieseniss A., Katschinski D.M., Jans D.C., Jakobs S., Warscheid B., Rehling P., Deckers M.
    Cell Metab. 15:336-347(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ASSOCIATION WITH THE RESPIRATORY CHAIN COMPLEX III/COMPLEX IV SUPERCOMPLEX.
  9. "Rcf1 and Rcf2, members of the hypoxia-induced gene 1 protein family, are critical components of the mitochondrial cytochrome bc1-cytochrome c oxidase supercomplex."
    Strogolova V., Furness A., Robb-McGrath M., Garlich J., Stuart R.A.
    Mol. Cell. Biol. 32:1363-1373(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ASSOCIATION WITH THE RESPIRATORY CHAIN COMPLEX III/COMPLEX IV SUPERCOMPLEX.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRCF2_YEAST
AccessioniPrimary (citable) accession number: P53721
Secondary accession number(s): D6W1J3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 11, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 12000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.