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Protein

tRNA-dihydrouridine(20) synthase [NAD(P)+]

Gene

SMM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U20 in cytoplasmic tRNAs.2 Publications

Miscellaneous

Present with 2650 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

5,6-dihydrouracil(20) in tRNA + NAD(P)+ = uracil(20) in tRNA + NAD(P)H.

Cofactori

FMNBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei88FMNBy similarity1
Active sitei117Proton donorBy similarity1
Binding sitei160FMNBy similarity1
Binding sitei188FMNBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 14FMNBy similarity3
Nucleotide bindingi222 – 224FMNBy similarity3
Nucleotide bindingi249 – 250FMNBy similarity2

GO - Molecular functioni

GO - Biological processi

  • tRNA modification Source: SGD
  • tRNA processing Source: SGD

Keywordsi

Molecular functionOxidoreductase
Biological processtRNA processing
LigandFlavoprotein, FMN, NAD, NADP

Enzyme and pathway databases

BioCyciMetaCyc:G3O-33331-MONOMER
YEAST:G3O-33331-MONOMER
BRENDAi1.3.1.91 984

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-dihydrouridine(20) synthase [NAD(P)+] (EC:1.3.1.91)
Alternative name(s):
Protein SMM1
tRNA-dihydrouridine synthase 2
Gene namesi
Name:SMM1
Synonyms:DUS2
Ordered Locus Names:YNR015W
ORF Names:N2065
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNR015W
SGDiS000005298 SMM1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001621541 – 384tRNA-dihydrouridine(20) synthase [NAD(P)+]Add BLAST384

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP53720
PaxDbiP53720
PRIDEiP53720

PTM databases

iPTMnetiP53720

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi35840, 80 interactors
DIPiDIP-4102N
IntActiP53720, 5 interactors
STRINGi4932.YNR015W

Structurei

3D structure databases

ProteinModelPortaliP53720
SMRiP53720
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Dus family. Dus2 subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00550000075019
HOGENOMiHOG000195580
InParanoidiP53720
KOiK05543
OMAiTSGVIKM
OrthoDBiEOG092C29SE

Family and domain databases

CDDicd02801 DUS_like_FMN, 1 hit
Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR035587 DUS-like_FMN-bd
IPR001269 tRNA_hU_synthase
IPR018517 tRNA_hU_synthase_CS
PANTHERiPTHR11082 PTHR11082, 1 hit
PfamiView protein in Pfam
PF01207 Dus, 1 hit
PIRSFiPIRSF006621 Dus, 1 hit
PROSITEiView protein in PROSITE
PS01136 UPF0034, 1 hit

Sequencei

Sequence statusi: Complete.

P53720-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTYAGKLVL APMVRAGELP TRLMALAHGA DLVWSPEIID KKLIQCVRKE
60 70 80 90 100
NTALQTVDYV VPSKVQTRPE TLVFRTYPKL ESSKLIFQIG SASPALATQA
110 120 130 140 150
ALKVINDVSG IDINAGCPKH FSIHSGMGSA LLRTPDTLCL ILKELVKNVG
160 170 180 190 200
NPHSKPISVK IRLLDTKQDT LQLVKRLCAT GITNLTVHCR KTEMRNREQP
210 220 230 240 250
ITDYIAEIYE ICQANNVSLI VNGAIRDRSH FHDLQANHWK NTNIGGMIAE
260 270 280 290 300
CAERDPTVFD HTSKPSEDGP SWVVACREFI QWATKFDNHI GNTKYMLSRI
310 320 330 340 350
VPGKSVFFQY FARCKSPEEV SFVLKQLNDD GSAQTDPSEY LENCRAQEKA
360 370 380
LKNANAIAKQ KRKQTDHIGS DTKKQKVVPL PTDI
Length:384
Mass (Da):42,816
Last modified:October 1, 1996 - v1
Checksum:i637EEC05DD4F9D6D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91816 Genomic DNA Translation: CAA62924.1
Z71630 Genomic DNA Translation: CAA96293.1
Z71631 Genomic DNA Translation: CAA96295.1
BK006947 Genomic DNA Translation: DAA10556.1
PIRiS63345
RefSeqiNP_014412.1, NM_001183192.1

Genome annotation databases

EnsemblFungiiYNR015W; YNR015W; YNR015W
GeneIDi855749
KEGGisce:YNR015W

Similar proteinsi

Entry informationi

Entry nameiDUS2_YEAST
AccessioniPrimary (citable) accession number: P53720
Secondary accession number(s): D6W1J0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health