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P53720 (DUS2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-dihydrouridine(20) synthase [NAD(P)+]

EC=1.3.1.91
Alternative name(s):
Protein SMM1
tRNA-dihydrouridine synthase 2
Gene names
Name:SMM1
Synonyms:DUS2
Ordered Locus Names:YNR015W
ORF Names:N2065
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length384 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U20 in cytoplasmic tRNAs. Ref.4 Ref.7

Catalytic activity

5,6-dihydrouracil20 in tRNA + NAD(P)+ = uracil20 in tRNA + NAD(P)H.

Cofactor

FAD By similarity.

Subunit structure

Monomer.

Subcellular location

Cytoplasm. Nucleus Ref.5.

Post-translational modification

N-glycosylated.

Miscellaneous

Present with 2650 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the Dus family. Dus2 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 384384tRNA-dihydrouridine(20) synthase [NAD(P)+]
PRO_0000162154

Sequences

Sequence LengthMass (Da)Tools
P53720 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 637EEC05DD4F9D6D

FASTA38442,816
        10         20         30         40         50         60 
MVTYAGKLVL APMVRAGELP TRLMALAHGA DLVWSPEIID KKLIQCVRKE NTALQTVDYV 

        70         80         90        100        110        120 
VPSKVQTRPE TLVFRTYPKL ESSKLIFQIG SASPALATQA ALKVINDVSG IDINAGCPKH 

       130        140        150        160        170        180 
FSIHSGMGSA LLRTPDTLCL ILKELVKNVG NPHSKPISVK IRLLDTKQDT LQLVKRLCAT 

       190        200        210        220        230        240 
GITNLTVHCR KTEMRNREQP ITDYIAEIYE ICQANNVSLI VNGAIRDRSH FHDLQANHWK 

       250        260        270        280        290        300 
NTNIGGMIAE CAERDPTVFD HTSKPSEDGP SWVVACREFI QWATKFDNHI GNTKYMLSRI 

       310        320        330        340        350        360 
VPGKSVFFQY FARCKSPEEV SFVLKQLNDD GSAQTDPSEY LENCRAQEKA LKNANAIAKQ 

       370        380 
KRKQTDHIGS DTKKQKVVPL PTDI 

« Hide

References

« Hide 'large scale' references
[1]"Identification of three nuclear genes which in high copy number suppress a mitochondrial mutation in the tRNA(Asp) gene in Saccharomyces cerevisiae."
Rinaldi T., Lande R., Bolotin-Fukuhara M., Frontali L.
Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: R100.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA."
Xing F., Martzen M.R., Phizicky E.M.
RNA 8:370-381(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs."
Xing F., Hiley S.L., Hughes T.R., Phizicky E.M.
J. Biol. Chem. 279:17850-17860(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes."
Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H.
Mol. Syst. Biol. 5:308-308(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X91816 Genomic DNA. Translation: CAA62924.1.
Z71630 Genomic DNA. Translation: CAA96293.1.
Z71631 Genomic DNA. Translation: CAA96295.1.
BK006947 Genomic DNA. Translation: DAA10556.1.
PIRS63345.
RefSeqNP_014412.1. NM_001183192.1.

3D structure databases

ProteinModelPortalP53720.
SMRP53720. Positions 7-227.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35840. 45 interactions.
DIPDIP-4102N.
MINTMINT-551094.
STRING4932.YNR015W.

Proteomic databases

MaxQBP53720.
PaxDbP53720.
PeptideAtlasP53720.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNR015W; YNR015W; YNR015W.
GeneID855749.
KEGGsce:YNR015W.

Organism-specific databases

CYGDYNR015w.
SGDS000005298. SMM1.

Phylogenomic databases

eggNOGCOG0042.
GeneTreeENSGT00550000075019.
HOGENOMHOG000195580.
KOK05543.
OMAYGVDGAM.
OrthoDBEOG7J9W16.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-16677.
YEAST:G3O-33331-MONOMER.

Gene expression databases

GenevestigatorP53720.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
[Graphical view]
PANTHERPTHR11082. PTHR11082. 1 hit.
PfamPF01207. Dus. 1 hit.
[Graphical view]
PIRSFPIRSF006621. Dus. 1 hit.
PROSITEPS01136. UPF0034. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio980160.
PROP53720.

Entry information

Entry nameDUS2_YEAST
AccessionPrimary (citable) accession number: P53720
Secondary accession number(s): D6W1J0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families