SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P53720

- DUS2_YEAST

UniProt

P53720 - DUS2_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

tRNA-dihydrouridine(20) synthase [NAD(P)+]

Gene
SMM1, DUS2, YNR015W, N2065
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U20 in cytoplasmic tRNAs.2 Publications

Catalytic activityi

5,6-dihydrouracil(20) in tRNA + NAD(P)+ = uracil(20) in tRNA + NAD(P)H.

Cofactori

FAD By similarity.

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. tRNA dihydrouridine synthase activity Source: SGD

GO - Biological processi

  1. tRNA dihydrouridine synthesis Source: GOC
  2. tRNA modification Source: SGD
  3. tRNA processing Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16677.
YEAST:G3O-33331-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-dihydrouridine(20) synthase [NAD(P)+] (EC:1.3.1.91)
Alternative name(s):
Protein SMM1
tRNA-dihydrouridine synthase 2
Gene namesi
Name:SMM1
Synonyms:DUS2
Ordered Locus Names:YNR015W
ORF Names:N2065
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNR015w.
SGDiS000005298. SMM1.

Subcellular locationi

Cytoplasm. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 384384tRNA-dihydrouridine(20) synthase [NAD(P)+]PRO_0000162154Add
BLAST

Post-translational modificationi

N-glycosylated.

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP53720.
PaxDbiP53720.
PeptideAtlasiP53720.

Expressioni

Gene expression databases

GenevestigatoriP53720.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi35840. 45 interactions.
DIPiDIP-4102N.
MINTiMINT-551094.
STRINGi4932.YNR015W.

Structurei

3D structure databases

ProteinModelPortaliP53720.
SMRiP53720. Positions 7-227.

Family & Domainsi

Sequence similaritiesi

Belongs to the Dus family. Dus2 subfamily.

Phylogenomic databases

eggNOGiCOG0042.
GeneTreeiENSGT00550000075019.
HOGENOMiHOG000195580.
KOiK05543.
OMAiYGVDGAM.
OrthoDBiEOG7J9W16.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
[Graphical view]
PANTHERiPTHR11082. PTHR11082. 1 hit.
PfamiPF01207. Dus. 1 hit.
[Graphical view]
PIRSFiPIRSF006621. Dus. 1 hit.
PROSITEiPS01136. UPF0034. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53720-1 [UniParc]FASTAAdd to Basket

« Hide

MVTYAGKLVL APMVRAGELP TRLMALAHGA DLVWSPEIID KKLIQCVRKE    50
NTALQTVDYV VPSKVQTRPE TLVFRTYPKL ESSKLIFQIG SASPALATQA 100
ALKVINDVSG IDINAGCPKH FSIHSGMGSA LLRTPDTLCL ILKELVKNVG 150
NPHSKPISVK IRLLDTKQDT LQLVKRLCAT GITNLTVHCR KTEMRNREQP 200
ITDYIAEIYE ICQANNVSLI VNGAIRDRSH FHDLQANHWK NTNIGGMIAE 250
CAERDPTVFD HTSKPSEDGP SWVVACREFI QWATKFDNHI GNTKYMLSRI 300
VPGKSVFFQY FARCKSPEEV SFVLKQLNDD GSAQTDPSEY LENCRAQEKA 350
LKNANAIAKQ KRKQTDHIGS DTKKQKVVPL PTDI 384
Length:384
Mass (Da):42,816
Last modified:October 1, 1996 - v1
Checksum:i637EEC05DD4F9D6D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X91816 Genomic DNA. Translation: CAA62924.1.
Z71630 Genomic DNA. Translation: CAA96293.1.
Z71631 Genomic DNA. Translation: CAA96295.1.
BK006947 Genomic DNA. Translation: DAA10556.1.
PIRiS63345.
RefSeqiNP_014412.1. NM_001183192.1.

Genome annotation databases

EnsemblFungiiYNR015W; YNR015W; YNR015W.
GeneIDi855749.
KEGGisce:YNR015W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X91816 Genomic DNA. Translation: CAA62924.1 .
Z71630 Genomic DNA. Translation: CAA96293.1 .
Z71631 Genomic DNA. Translation: CAA96295.1 .
BK006947 Genomic DNA. Translation: DAA10556.1 .
PIRi S63345.
RefSeqi NP_014412.1. NM_001183192.1.

3D structure databases

ProteinModelPortali P53720.
SMRi P53720. Positions 7-227.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35840. 45 interactions.
DIPi DIP-4102N.
MINTi MINT-551094.
STRINGi 4932.YNR015W.

Proteomic databases

MaxQBi P53720.
PaxDbi P53720.
PeptideAtlasi P53720.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNR015W ; YNR015W ; YNR015W .
GeneIDi 855749.
KEGGi sce:YNR015W.

Organism-specific databases

CYGDi YNR015w.
SGDi S000005298. SMM1.

Phylogenomic databases

eggNOGi COG0042.
GeneTreei ENSGT00550000075019.
HOGENOMi HOG000195580.
KOi K05543.
OMAi YGVDGAM.
OrthoDBi EOG7J9W16.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-16677.
YEAST:G3O-33331-MONOMER.

Miscellaneous databases

NextBioi 980160.
PROi P53720.

Gene expression databases

Genevestigatori P53720.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
[Graphical view ]
PANTHERi PTHR11082. PTHR11082. 1 hit.
Pfami PF01207. Dus. 1 hit.
[Graphical view ]
PIRSFi PIRSF006621. Dus. 1 hit.
PROSITEi PS01136. UPF0034. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of three nuclear genes which in high copy number suppress a mitochondrial mutation in the tRNA(Asp) gene in Saccharomyces cerevisiae."
    Rinaldi T., Lande R., Bolotin-Fukuhara M., Frontali L.
    Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: R100.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA."
    Xing F., Martzen M.R., Phizicky E.M.
    RNA 8:370-381(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs."
    Xing F., Hiley S.L., Hughes T.R., Phizicky E.M.
    J. Biol. Chem. 279:17850-17860(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes."
    Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H.
    Mol. Syst. Biol. 5:308-308(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDUS2_YEAST
AccessioniPrimary (citable) accession number: P53720
Secondary accession number(s): D6W1J0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2650 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

Similar proteinsi