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P53720

- DUS2_YEAST

UniProt

P53720 - DUS2_YEAST

Protein

tRNA-dihydrouridine(20) synthase [NAD(P)+]

Gene

SMM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U20 in cytoplasmic tRNAs.2 Publications

    Catalytic activityi

    5,6-dihydrouracil(20) in tRNA + NAD(P)+ = uracil(20) in tRNA + NAD(P)H.

    Cofactori

    FAD.By similarity

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. tRNA dihydrouridine synthase activity Source: SGD

    GO - Biological processi

    1. tRNA dihydrouridine synthesis Source: GOC
    2. tRNA modification Source: SGD
    3. tRNA processing Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    FAD, Flavoprotein, NAD, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16677.
    YEAST:G3O-33331-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-dihydrouridine(20) synthase [NAD(P)+] (EC:1.3.1.91)
    Alternative name(s):
    Protein SMM1
    tRNA-dihydrouridine synthase 2
    Gene namesi
    Name:SMM1
    Synonyms:DUS2
    Ordered Locus Names:YNR015W
    ORF Names:N2065
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNR015w.
    SGDiS000005298. SMM1.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 384384tRNA-dihydrouridine(20) synthase [NAD(P)+]PRO_0000162154Add
    BLAST

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP53720.
    PaxDbiP53720.
    PeptideAtlasiP53720.

    Expressioni

    Gene expression databases

    GenevestigatoriP53720.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    BioGridi35840. 45 interactions.
    DIPiDIP-4102N.
    MINTiMINT-551094.
    STRINGi4932.YNR015W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53720.
    SMRiP53720. Positions 7-227.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Dus family. Dus2 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0042.
    GeneTreeiENSGT00550000075019.
    HOGENOMiHOG000195580.
    KOiK05543.
    OMAiYGVDGAM.
    OrthoDBiEOG7J9W16.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR001269. tRNA_hU_synthase.
    IPR018517. tRNA_hU_synthase_CS.
    [Graphical view]
    PANTHERiPTHR11082. PTHR11082. 1 hit.
    PfamiPF01207. Dus. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006621. Dus. 1 hit.
    PROSITEiPS01136. UPF0034. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53720-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVTYAGKLVL APMVRAGELP TRLMALAHGA DLVWSPEIID KKLIQCVRKE    50
    NTALQTVDYV VPSKVQTRPE TLVFRTYPKL ESSKLIFQIG SASPALATQA 100
    ALKVINDVSG IDINAGCPKH FSIHSGMGSA LLRTPDTLCL ILKELVKNVG 150
    NPHSKPISVK IRLLDTKQDT LQLVKRLCAT GITNLTVHCR KTEMRNREQP 200
    ITDYIAEIYE ICQANNVSLI VNGAIRDRSH FHDLQANHWK NTNIGGMIAE 250
    CAERDPTVFD HTSKPSEDGP SWVVACREFI QWATKFDNHI GNTKYMLSRI 300
    VPGKSVFFQY FARCKSPEEV SFVLKQLNDD GSAQTDPSEY LENCRAQEKA 350
    LKNANAIAKQ KRKQTDHIGS DTKKQKVVPL PTDI 384
    Length:384
    Mass (Da):42,816
    Last modified:October 1, 1996 - v1
    Checksum:i637EEC05DD4F9D6D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X91816 Genomic DNA. Translation: CAA62924.1.
    Z71630 Genomic DNA. Translation: CAA96293.1.
    Z71631 Genomic DNA. Translation: CAA96295.1.
    BK006947 Genomic DNA. Translation: DAA10556.1.
    PIRiS63345.
    RefSeqiNP_014412.1. NM_001183192.1.

    Genome annotation databases

    EnsemblFungiiYNR015W; YNR015W; YNR015W.
    GeneIDi855749.
    KEGGisce:YNR015W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X91816 Genomic DNA. Translation: CAA62924.1 .
    Z71630 Genomic DNA. Translation: CAA96293.1 .
    Z71631 Genomic DNA. Translation: CAA96295.1 .
    BK006947 Genomic DNA. Translation: DAA10556.1 .
    PIRi S63345.
    RefSeqi NP_014412.1. NM_001183192.1.

    3D structure databases

    ProteinModelPortali P53720.
    SMRi P53720. Positions 7-227.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35840. 45 interactions.
    DIPi DIP-4102N.
    MINTi MINT-551094.
    STRINGi 4932.YNR015W.

    Proteomic databases

    MaxQBi P53720.
    PaxDbi P53720.
    PeptideAtlasi P53720.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNR015W ; YNR015W ; YNR015W .
    GeneIDi 855749.
    KEGGi sce:YNR015W.

    Organism-specific databases

    CYGDi YNR015w.
    SGDi S000005298. SMM1.

    Phylogenomic databases

    eggNOGi COG0042.
    GeneTreei ENSGT00550000075019.
    HOGENOMi HOG000195580.
    KOi K05543.
    OMAi YGVDGAM.
    OrthoDBi EOG7J9W16.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-16677.
    YEAST:G3O-33331-MONOMER.

    Miscellaneous databases

    NextBioi 980160.
    PROi P53720.

    Gene expression databases

    Genevestigatori P53720.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR001269. tRNA_hU_synthase.
    IPR018517. tRNA_hU_synthase_CS.
    [Graphical view ]
    PANTHERi PTHR11082. PTHR11082. 1 hit.
    Pfami PF01207. Dus. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006621. Dus. 1 hit.
    PROSITEi PS01136. UPF0034. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of three nuclear genes which in high copy number suppress a mitochondrial mutation in the tRNA(Asp) gene in Saccharomyces cerevisiae."
      Rinaldi T., Lande R., Bolotin-Fukuhara M., Frontali L.
      Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: R100.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA."
      Xing F., Martzen M.R., Phizicky E.M.
      RNA 8:370-381(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs."
      Xing F., Hiley S.L., Hughes T.R., Phizicky E.M.
      J. Biol. Chem. 279:17850-17860(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes."
      Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H.
      Mol. Syst. Biol. 5:308-308(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiDUS2_YEAST
    AccessioniPrimary (citable) accession number: P53720
    Secondary accession number(s): D6W1J0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2650 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3