Skip Header

 
Contribute Send feedback

Reviewed, UniProtKB/Swiss-Prot P53708 (ITA8_HUMAN)

Last modified November 25, 2008. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Integrin alpha-8
Cleaved into the following 2 chains:
    1- Recommended name:
            Integrin alpha-8 heavy chain
    2- Recommended name:
            Integrin alpha-8 light chain
Gene names
Name: ITGA8
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Hide | Top

Protein attributes

Sequence length1063 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

Integrin alpha-8/beta-1 functions in the genesis of kidney and probably of other organs by regulating the recruitment of mesenchymal cells into epithelial structures. It recognizes the sequence R-G-D in a wide array of ligands including TNC, FN1, SPP1 TGFB1, TGFB3 and VTN. NPNT is probably its functional ligand in kidney genesis. Neuronal receptor for TNC it mediates cell-cell interactions and regulates neurite outgrowth of sensory and motor neurons.

Subunit structure

Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-8 associates with beta-1.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Expressed in mesenchymal cells, including alveolar myofibroblasts, kidney mesangial cells and hepatic stellar cells and vascular and visceral smooth muscle (at protein level).

Sequence similarities

Belongs to the integrin alpha chain family.

Contains 7 FG-GAP repeats.

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3838 Potential
Chain39 – 10631025Integrin alpha-8
PRO_0000016310
Chain39 – 906868Integrin alpha-8 heavy chain Potential
PRO_0000016311
Chain907 – 1063157Integrin alpha-8 light chain Potential
PRO_0000016312

Regions

Topological domain39 – 1012974Extracellular Potential
Transmembrane1013 – 103321 Potential
Topological domain1034 – 106330Cytoplasmic Potential
Repeat54 – 11764FG-GAP 1
Repeat133 – 19866FG-GAP 2
Repeat199 – 26365FG-GAP 3
Repeat264 – 31754FG-GAP 4
Repeat318 – 38366FG-GAP 5
Repeat384 – 44360FG-GAP 6
Repeat447 – 50155FG-GAP 7
Calcium binding329 – 3379 Potential
Calcium binding395 – 4039 Potential
Calcium binding459 – 4679 Potential
Motif455 – 4573Cell attachment site Potential

Amino acid modifications

Glycosylation811N-linked (GlcNAc...) Potential
Glycosylation1221N-linked (GlcNAc...) Potential
Glycosylation1771N-linked (GlcNAc...) Potential
Glycosylation2391N-linked (GlcNAc...) Potential
Glycosylation3021N-linked (GlcNAc...) Potential
Glycosylation3111N-linked (GlcNAc...) Potential
Glycosylation5041N-linked (GlcNAc...) Potential
Glycosylation6011N-linked (GlcNAc...) Potential
Glycosylation6051N-linked (GlcNAc...) Potential
Glycosylation7191N-linked (GlcNAc...) Potential
Glycosylation7371N-linked (GlcNAc...) Potential
Glycosylation7531N-linked (GlcNAc...) Potential
Glycosylation7801N-linked (GlcNAc...) Potential
Glycosylation8961N-linked (GlcNAc...) Potential
Glycosylation9231N-linked (GlcNAc...) Potential
Glycosylation10051N-linked (GlcNAc...) Potential
Disulfide bond96 ↔ 106 By similarity
Disulfide bond150 ↔ 171 By similarity
Disulfide bond187 ↔ 200 By similarity
Disulfide bond507 ↔ 518 By similarity
Disulfide bond524 ↔ 580 By similarity
Disulfide bond641 ↔ 647 By similarity
Disulfide bond713 ↔ 726 By similarity
Disulfide bond867 ↔ 924Interchain (between heavy and light chains) By similarity
Disulfide bond929 ↔ 934 By similarity

Natural variations

Natural variant2161V → L: dbSNP rs7895372.
VAR_018673
Natural variant5671V → L
VAR_034682
Natural variant5771S → F: dbSNP rs2298033.
VAR_018674
Natural variant5811Q → P: dbSNP rs9333269.
VAR_018675
Natural variant7481R → H: dbSNP rs9333174.
VAR_018676
Natural variant9931I → V: dbSNP rs9333241.
VAR_018677
Natural variant9941A → V: dbSNP rs1041135.
VAR_018678

Experimental info

Sequence conflict471T → A in AAA93514. Ref.3
Sequence conflict1661D → G in AAA93514. Ref.3
Sequence conflict1881R → G in AAA93514. Ref.3
Sequence conflict3041T → Y in AAA93514. Ref.3

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P53708-1 [UniParc].

Last modified June 7, 2004. Version 2.
Checksum: 9D0F9FBDBB967267

FASTA1,063117,446
        10         20         30         40         50         60 
MSPGASRGPR GSQAPLIAPL CCAAAALGML LWSPACQAFN LDVEKLTVYS GPKGSYFGYA 

        70         80         90        100        110        120 
VDFHIPDART ASVLVGAPKA NTSQPDIVEG GAVYYCPWPA EGSAQCRQIP FDTTNNRKIR 

       130        140        150        160        170        180 
VNGTKEPIEF KSNQWFGATV KAHKGKVVAC APLYHWRTLK PTPEKDPVGT CYVAIQNFSA 

       190        200        210        220        230        240 
YAEFSPCRNS NADPEGQGYC QAGFSLDFYK NGDLIVGGPG SFYWQGQVIT ASVADIIANY 

       250        260        270        280        290        300 
SFKDILRKLA GEKQTEVAPA SYDDSYLGYS VAAGEFTGDS QQELVAGIPR GAQNFGYVSI 

       310        320        330        340        350        360 
INSTDMTFIQ NFTGEQMASY FGYTVVVSDV NSDGLDDVLV GAPLFMEREF ESNPREVGQI 

       370        380        390        400        410        420 
YLYLQVSSLL FRDPQILTGT ETFGRFGSAM AHLGDLNQDG YNDIAIGVPF AGKDQRGKVL 

       430        440        450        460        470        480 
IYNGNKDGLN TKPSQVLQGV WASHAVPSGF GFTLRGDSDI DKNDYPDLIV GAFGTGKVAV 

       490        500        510        520        530        540 
YRARPVVTVD AQLLLHPMII NLENKTCQVP DSMTSAACFS LRVCASVTGQ SIANTIVLMA 

       550        560        570        580        590        600 
EVQLDSLKQK GAIKRTLFLD NHQAHRVFPL VIKRQKSHQC QDFIVYLRDE TEFRDKLSPI 

       610        620        630        640        650        660 
NISLNYSLDE STFKEGLEVK PILNYYRENI VSEQAHILVD CGEDNLCVPD LKLSARPDKH 

       670        680        690        700        710        720 
QVIIGDENHL MLIINARNEG EGAYEAELFV MIPEEADYVG IERNNKGFRP LSCEYKMENV 

       730        740        750        760        770        780 
TRMVVCDLGN PMVSGTNYSL GLRFAVPRLE KTNMSINFDL QIRSSNKDNP DSNFVSLQIN 

       790        800        810        820        830        840 
ITAVAQVEIR GVSHPPQIVL PIHNWEPEEE PHKEEEVGPL VEHIYELHNI GPSTISDTIL 

       850        860        870        880        890        900 
EVGWPFSARD EFLLYIFHIQ TLGPLQCQPN PNINPQDIKP AASPEDTPEL SAFLRNSTIP 

       910        920        930        940        950        960 
HLVRKRDVHV VEFHRQSPAK ILNCTNIECL QISCAVGRLE GGESAVLKVR SRLWAHTFLQ 

       970        980        990       1000       1010       1020 
RKNDPYALAS LVSFEVKKMP YTDQPAKLPE GSIAIKTSVI WATPNVSFSI PLWVIILAIL 

      1030       1040       1050       1060 
LGLLVLAILT LALWKCGFFD RARPPQEDMT DREQLTNDKT PEA

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]SeattleSNPs program for genomic applications
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS LEU-216; PHE-577; PRO-581; HIS-748; VAL-993 AND VAL-994.
[2]"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. expand/collapse author list , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
Nature 429:375-381(2004) [PubMed: 15164054] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT VAL-994.
[3]"Sequence and tissue distribution of the human integrin alpha 8 subunit: a beta 1-associated alpha subunit expressed in smooth muscle cells."
Schnapp L.M., Breuss J.M., Ramos D.M., Sheppard D., Pytela R.
J. Cell Sci. 108:537-544(1995) [PubMed: 7768999] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 39-1063, TISSUE SPECIFICITY.
[4]"Alpha8 integrin in glomerular mesangial cells and in experimental glomerulonephritis."
Hartner A., Schoecklmann H., Proels F., Mueller U., Sterzel R.B.
Kidney Int. 56:1468-1480(1999) [PubMed: 10504498] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"Integrin alpha8beta1 mediates adhesion to LAP-TGFbeta1."
Lu M., Munger J.S., Steadele M., Busald C., Tellier M., Schnapp L.M.
J. Cell Sci. 115:4641-4648(2002) [PubMed: 12415008] [Abstract]
Cited for: FUNCTION.
[6]"Integrin alpha8beta1-fibronectin interactions promote cell survival via PI3 kinase pathway."
Farias E., Lu M., Li X., Schnapp L.M.
Biochem. Biophys. Res. Commun. 329:305-311(2005) [PubMed: 15721307] [Abstract]
Cited for: FUNCTION.
[7]"Genomic organization and sequence variation of the human integrin subunit alpha8 gene (ITGA8)."
Ekwa-Ekoka C., Diaz G.A., Carlson C., Hasegawa T., Samudrala R., Lim K.-C., Yabu J.M., Levy B., Schnapp L.M.
Matrix Biol. 23:487-496(2004) [PubMed: 15579315] [Abstract]
Cited for: VARIANTS LEU-567; PHE-577; PRO-581; HIS-748; VAL-993 AND VAL-994.

Hide | Top

Web resources

SeattleSNPs

Hide | Top

Cross-references

Sequence databases

AY371697 Genomic DNA. Translation: AAQ56848.2.
AL359645, AL590636 Genomic DNA. Translation: CAH73424.1.
AL590636, AL359645 Genomic DNA. Translation: CAI14955.1.
L36531 mRNA. Translation: AAA93514.1.
RefSeqNP_003629.1.
UniGeneHs.171311

3D structure databases

HSSPHSSP built from PDB template 1L5G based on UniProtKB P06756.
ModBaseSearch...

PTM databases

PhosphoSiteP53708.

Genome annotation databases

EnsemblENSG00000077943. Homo sapiens. [Contig view]
GeneID8516.
KEGGhsa:8516.

Organism-specific databases

H-InvDBHIX0035424.
HGNCHGNC:6144. ITGA8.
HPAHPA003432.
MIM604063. gene.
PharmGKBPA29944.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMP53708.
HOVERGENP53708.

Gene expression databases

ArrayExpressP53708.
CleanExHS_ITGA8.
GermOnlineENSG00000077943. Homo sapiens.

Family and domain databases

InterProIPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.