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P53704

- GFA1_CANAL

UniProt

P53704 - GFA1_CANAL

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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

GFA1

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins).

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: CGD

GO - Biological processi

  1. cellular response to drug Source: CGD
  2. cell wall chitin biosynthetic process Source: CGD
  3. chitin biosynthetic process Source: CGD
  4. glucosamine biosynthetic process Source: CGD
  5. glutamine metabolic process Source: UniProtKB-KW
  6. hyphal growth Source: CGD
  7. protein N-linked glycosylation Source: CGD
  8. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13172.
UniPathwayiUPA00113; UER00528.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Short name:
GFAT
Alternative name(s):
D-fructose-6-phosphate amidotransferase
Hexosephosphate aminotransferase
Gene namesi
Name:GFA1
ORF Names:CaO19.1618
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
ProteomesiUP000000559: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0006344. GFA1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 713712Glutamine--fructose-6-phosphate aminotransferase [isomerizing]PRO_0000135287Add
BLAST

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi5476.CAL0006344.

Structurei

Secondary structure

1
713
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi354 – 3607Combined sources
Helixi362 – 3709Combined sources
Turni371 – 3733Combined sources
Turni376 – 3794Combined sources
Turni384 – 3863Combined sources
Helixi387 – 3893Combined sources
Helixi390 – 3945Combined sources
Beta strandi396 – 4038Combined sources
Helixi405 – 42218Combined sources
Beta strandi426 – 4305Combined sources
Helixi431 – 4366Combined sources
Beta strandi445 – 45410Combined sources
Helixi457 – 46812Combined sources
Beta strandi472 – 4798Combined sources
Helixi483 – 4875Combined sources
Beta strandi488 – 4936Combined sources
Beta strandi501 – 5033Combined sources
Helixi506 – 52217Combined sources
Turni523 – 5253Combined sources
Turni527 – 5293Combined sources
Helixi530 – 55021Combined sources
Helixi551 – 5533Combined sources
Helixi554 – 5629Combined sources
Helixi564 – 5674Combined sources
Beta strandi569 – 5757Combined sources
Helixi577 – 5793Combined sources
Helixi580 – 59415Combined sources
Beta strandi597 – 6026Combined sources
Beta strandi620 – 6234Combined sources
Helixi626 – 6283Combined sources
Helixi631 – 64212Combined sources
Beta strandi648 – 6525Combined sources
Beta strandi665 – 6695Combined sources
Helixi674 – 6763Combined sources
Helixi677 – 69519Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2POCX-ray1.80A/B/C/D347-713[»]
2PUTX-ray1.90A/B/C/D347-713[»]
2PUVX-ray1.90A/B/C/D347-713[»]
2PUWX-ray3.15A/B347-713[»]
ProteinModelPortaliP53704.
SMRiP53704. Positions 350-701.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53704.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 316315Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST
Domaini389 – 528140SIS 1PROSITE-ProRule annotationAdd
BLAST
Domaini561 – 703143SIS 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation
Contains 2 SIS domains.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiCOG0449.
InParanoidiP53704.
KOiK00820.
OrthoDBiEOG7034RG.

Family and domain databases

Gene3Di3.60.20.10. 2 hits.
InterProiIPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF00310. GATase_2. 1 hit.
PF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 2 hits.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53704-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCGIFGYVNF LVDKSRGEII DNLIEGLQRL EYRGYDSAGI AVDGKLTKDP
60 70 80 90 100
SNGDEEYMDS IIVKTTGKVK VLKQKIIDDQ IDRSAIFDNH VGIAHTRWAT
110 120 130 140 150
HGQPKTENCH PHKSDPKGEF IVVHNGIITN YAALRKYLLS KGHVFESETD
160 170 180 190 200
TECIAKLFKH FYDLNVKAGV FPDLNELTKQ VLHELEGSYG LLVKSYHYPG
210 220 230 240 250
EVCGTRKGSP LLVGVKTDKK LKVDFVDVEF EAQQQHRPQQ PQINHNGATS
260 270 280 290 300
AAELGFIPVA PGEQNLRTSQ SRAFLSEDDL PMPVEFFLSS DPASVVQHTK
310 320 330 340 350
KVLFLEDDDI AHIYDGELRI HRASTKSAGE STVRPIQTLE MELNEIMKGP
360 370 380 390 400
YKHFMQKEIF EQPDSAFNTM RGRIDFENCV VTLGGLKSWL STIRRCRRII
410 420 430 440 450
MIACGTSYHS CLATRSIFEE LTEIPVSVEL ASDFLDRRSP VFRDDTCVFV
460 470 480 490 500
SQSGETADSI LALQYCLERG ALTVGIVNSV GSSMSRQTHC GVHINAGPEI
510 520 530 540 550
GVASTKAYTS QYIALVMFAL SLSNDSISRK GRHEEIIKGL QKIPEQIKQV
560 570 580 590 600
LKLENKIKDL CNSSLNDQKS LLLLGRGYQF ATALEGALKI KEISYMHSEG
610 620 630 640 650
VLAGELKHGI LALVDEDLPI IAFATRDSLF PKVMSAIEQV TARDGRPIVI
660 670 680 690 700
CNEGDAIISN DKVHTTLEVP ETVDCLQGLL NVIPLQLISY WLAVNRGIDV
710
DFPRNLAKSV TVE
Length:713
Mass (Da):79,268
Last modified:January 23, 2007 - v2
Checksum:i595EB8E90D704D53
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94753 Genomic DNA. Translation: CAA64380.1.
AACQ01000013 Genomic DNA. Translation: EAL02907.1.
PIRiJC6012.
RefSeqiXP_721697.1. XM_716604.1.

Genome annotation databases

GeneIDi3636557.
KEGGical:CaO19.1618.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94753 Genomic DNA. Translation: CAA64380.1 .
AACQ01000013 Genomic DNA. Translation: EAL02907.1 .
PIRi JC6012.
RefSeqi XP_721697.1. XM_716604.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2POC X-ray 1.80 A/B/C/D 347-713 [» ]
2PUT X-ray 1.90 A/B/C/D 347-713 [» ]
2PUV X-ray 1.90 A/B/C/D 347-713 [» ]
2PUW X-ray 3.15 A/B 347-713 [» ]
ProteinModelPortali P53704.
SMRi P53704. Positions 350-701.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5476.CAL0006344.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3636557.
KEGGi cal:CaO19.1618.

Organism-specific databases

CGDi CAL0006344. GFA1.

Phylogenomic databases

eggNOGi COG0449.
InParanoidi P53704.
KOi K00820.
OrthoDBi EOG7034RG.

Enzyme and pathway databases

UniPathwayi UPA00113 ; UER00528 .
BioCyci MetaCyc:MONOMER-13172.

Miscellaneous databases

EvolutionaryTracei P53704.

Family and domain databases

Gene3Di 3.60.20.10. 2 hits.
InterProi IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view ]
PANTHERi PTHR10937:SF0. PTHR10937:SF0. 1 hit.
Pfami PF00310. GATase_2. 1 hit.
PF01380. SIS. 2 hits.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 2 hits.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of the GFA1 gene encoding the glutamine:fructose-6-phosphate amidotransferase of Candida albicans."
    Smith R.J., Milewski S., Brown A.J., Gooday G.W.
    J. Bacteriol. 178:2320-2327(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 10261 / CBS 2718 / NBRC 1061.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SC5314 / ATCC MYA-2876.
  3. "The crystal and solution studies of glucosamine-6-phosphate synthase from Candida albicans."
    Raczynska J., Olchowy J., Konariev P.V., Svergun D.I., Milewski S., Rypniewski W.
    J. Mol. Biol. 372:672-688(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 347-713, SUBUNIT.

Entry informationi

Entry nameiGFA1_CANAL
AccessioniPrimary (citable) accession number: P53704
Secondary accession number(s): Q5AJV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3