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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

GFA1

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins).

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (GFA1)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular response to drug Source: CGD
  • cell wall chitin biosynthetic process Source: CGD
  • chitin biosynthetic process Source: CGD
  • glucosamine biosynthetic process Source: CGD
  • glutamine metabolic process Source: UniProtKB-KW
  • hyphal growth Source: CGD
  • protein N-linked glycosylation Source: CGD
  • UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13172.
BRENDAi2.6.1.16. 1096.
UniPathwayiUPA00113; UER00528.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Short name:
GFAT
Alternative name(s):
D-fructose-6-phosphate amidotransferase
Hexosephosphate aminotransferase
Gene namesi
Name:GFA1
ORF Names:CaO19.1618
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
Proteomesi
  • UP000000559 Componenti: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0000176261. GFA1.
EuPathDBiFungiDB:C3_02280C_A.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001352872 – 713Glutamine--fructose-6-phosphate aminotransferase [isomerizing]Add BLAST712

Proteomic databases

PRIDEiP53704.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Chemistry databases

BindingDBiP53704.

Structurei

Secondary structure

1713
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi354 – 360Combined sources7
Helixi362 – 370Combined sources9
Turni371 – 373Combined sources3
Turni376 – 379Combined sources4
Turni384 – 386Combined sources3
Helixi387 – 389Combined sources3
Helixi390 – 394Combined sources5
Beta strandi396 – 403Combined sources8
Helixi405 – 422Combined sources18
Beta strandi426 – 430Combined sources5
Helixi431 – 436Combined sources6
Beta strandi445 – 454Combined sources10
Helixi457 – 468Combined sources12
Beta strandi472 – 479Combined sources8
Helixi483 – 487Combined sources5
Beta strandi488 – 493Combined sources6
Beta strandi501 – 503Combined sources3
Helixi506 – 522Combined sources17
Turni523 – 525Combined sources3
Turni527 – 529Combined sources3
Helixi530 – 550Combined sources21
Helixi551 – 553Combined sources3
Helixi554 – 562Combined sources9
Helixi564 – 567Combined sources4
Beta strandi569 – 575Combined sources7
Helixi577 – 579Combined sources3
Helixi580 – 594Combined sources15
Beta strandi597 – 602Combined sources6
Beta strandi607 – 609Combined sources3
Helixi610 – 612Combined sources3
Beta strandi620 – 623Combined sources4
Helixi626 – 628Combined sources3
Helixi631 – 642Combined sources12
Beta strandi648 – 652Combined sources5
Beta strandi665 – 669Combined sources5
Helixi674 – 676Combined sources3
Helixi677 – 695Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2POCX-ray1.80A/B/C/D347-713[»]
2PUTX-ray1.90A/B/C/D347-713[»]
2PUVX-ray1.90A/B/C/D347-713[»]
2PUWX-ray3.15A/B347-713[»]
ProteinModelPortaliP53704.
SMRiP53704.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53704.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 316Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST315
Domaini389 – 528SIS 1PROSITE-ProRule annotationAdd BLAST140
Domaini561 – 703SIS 2PROSITE-ProRule annotationAdd BLAST143

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation
Contains 2 SIS domains.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

InParanoidiP53704.
KOiK00820.
OrthoDBiEOG092C0ST1.

Family and domain databases

Gene3Di3.60.20.10. 2 hits.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 2 hits.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 2 hits.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53704-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIFGYVNF LVDKSRGEII DNLIEGLQRL EYRGYDSAGI AVDGKLTKDP
60 70 80 90 100
SNGDEEYMDS IIVKTTGKVK VLKQKIIDDQ IDRSAIFDNH VGIAHTRWAT
110 120 130 140 150
HGQPKTENCH PHKSDPKGEF IVVHNGIITN YAALRKYLLS KGHVFESETD
160 170 180 190 200
TECIAKLFKH FYDLNVKAGV FPDLNELTKQ VLHELEGSYG LLVKSYHYPG
210 220 230 240 250
EVCGTRKGSP LLVGVKTDKK LKVDFVDVEF EAQQQHRPQQ PQINHNGATS
260 270 280 290 300
AAELGFIPVA PGEQNLRTSQ SRAFLSEDDL PMPVEFFLSS DPASVVQHTK
310 320 330 340 350
KVLFLEDDDI AHIYDGELRI HRASTKSAGE STVRPIQTLE MELNEIMKGP
360 370 380 390 400
YKHFMQKEIF EQPDSAFNTM RGRIDFENCV VTLGGLKSWL STIRRCRRII
410 420 430 440 450
MIACGTSYHS CLATRSIFEE LTEIPVSVEL ASDFLDRRSP VFRDDTCVFV
460 470 480 490 500
SQSGETADSI LALQYCLERG ALTVGIVNSV GSSMSRQTHC GVHINAGPEI
510 520 530 540 550
GVASTKAYTS QYIALVMFAL SLSNDSISRK GRHEEIIKGL QKIPEQIKQV
560 570 580 590 600
LKLENKIKDL CNSSLNDQKS LLLLGRGYQF ATALEGALKI KEISYMHSEG
610 620 630 640 650
VLAGELKHGI LALVDEDLPI IAFATRDSLF PKVMSAIEQV TARDGRPIVI
660 670 680 690 700
CNEGDAIISN DKVHTTLEVP ETVDCLQGLL NVIPLQLISY WLAVNRGIDV
710
DFPRNLAKSV TVE
Length:713
Mass (Da):79,268
Last modified:January 23, 2007 - v2
Checksum:i595EB8E90D704D53
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94753 Genomic DNA. Translation: CAA64380.1.
AACQ01000013 Genomic DNA. Translation: EAL02907.1.
PIRiJC6012.
RefSeqiXP_721697.1. XM_716604.1.

Genome annotation databases

EnsemblFungiiEAL02907; EAL02907; CaO19.1618.
GeneIDi3636557.
KEGGical:CaO19.1618.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94753 Genomic DNA. Translation: CAA64380.1.
AACQ01000013 Genomic DNA. Translation: EAL02907.1.
PIRiJC6012.
RefSeqiXP_721697.1. XM_716604.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2POCX-ray1.80A/B/C/D347-713[»]
2PUTX-ray1.90A/B/C/D347-713[»]
2PUVX-ray1.90A/B/C/D347-713[»]
2PUWX-ray3.15A/B347-713[»]
ProteinModelPortaliP53704.
SMRiP53704.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiP53704.

Proteomic databases

PRIDEiP53704.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEAL02907; EAL02907; CaO19.1618.
GeneIDi3636557.
KEGGical:CaO19.1618.

Organism-specific databases

CGDiCAL0000176261. GFA1.
EuPathDBiFungiDB:C3_02280C_A.

Phylogenomic databases

InParanoidiP53704.
KOiK00820.
OrthoDBiEOG092C0ST1.

Enzyme and pathway databases

UniPathwayiUPA00113; UER00528.
BioCyciMetaCyc:MONOMER-13172.
BRENDAi2.6.1.16. 1096.

Miscellaneous databases

EvolutionaryTraceiP53704.

Family and domain databases

Gene3Di3.60.20.10. 2 hits.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 2 hits.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 2 hits.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGFA1_CANAL
AccessioniPrimary (citable) accession number: P53704
Secondary accession number(s): Q5AJV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.