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P53704

- GFA1_CANAL

UniProt

P53704 - GFA1_CANAL

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Protein
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Gene
GFA1, CaO19.1618
Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins).

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activity By similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: CGD

GO - Biological processi

  1. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway
  2. cell wall chitin biosynthetic process Source: CGD
  3. cellular response to drug Source: CGD
  4. chitin biosynthetic process Source: CGD
  5. glucosamine biosynthetic process Source: CGD
  6. glutamine metabolic process Source: UniProtKB-KW
  7. hyphal growth Source: CGD
  8. protein N-linked glycosylation Source: CGD
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13172.
UniPathwayiUPA00113; UER00528.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Short name:
GFAT
Alternative name(s):
D-fructose-6-phosphate amidotransferase
Hexosephosphate aminotransferase
Gene namesi
Name:GFA1
ORF Names:CaO19.1618
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida
ProteomesiUP000000559: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0006344. GFA1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 713712Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
PRO_0000135287Add
BLAST

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi5476.CAL0006344.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi354 – 3607
Helixi362 – 3709
Turni371 – 3733
Turni376 – 3794
Turni384 – 3863
Helixi387 – 3893
Helixi390 – 3945
Beta strandi396 – 4038
Helixi405 – 42218
Beta strandi426 – 4305
Helixi431 – 4366
Beta strandi445 – 45410
Helixi457 – 46812
Beta strandi472 – 4798
Helixi483 – 4875
Beta strandi488 – 4936
Beta strandi501 – 5033
Helixi506 – 52217
Turni523 – 5253
Turni527 – 5293
Helixi530 – 55021
Helixi551 – 5533
Helixi554 – 5629
Helixi564 – 5674
Beta strandi569 – 5757
Helixi577 – 5793
Helixi580 – 59415
Beta strandi597 – 6026
Beta strandi620 – 6234
Helixi626 – 6283
Helixi631 – 64212
Beta strandi648 – 6525
Beta strandi665 – 6695
Helixi674 – 6763
Helixi677 – 69519

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2POCX-ray1.80A/B/C/D347-713[»]
2PUTX-ray1.90A/B/C/D347-713[»]
2PUVX-ray1.90A/B/C/D347-713[»]
2PUWX-ray3.15A/B347-713[»]
ProteinModelPortaliP53704.
SMRiP53704. Positions 350-701.

Miscellaneous databases

EvolutionaryTraceiP53704.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 316315Glutamine amidotransferase type-2
Add
BLAST
Domaini389 – 528140SIS 1
Add
BLAST
Domaini561 – 703143SIS 2
Add
BLAST

Sequence similaritiesi

Contains 2 SIS domains.

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiCOG0449.
KOiK00820.
OrthoDBiEOG7034RG.

Family and domain databases

Gene3Di3.60.20.10. 2 hits.
InterProiIPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF00310. GATase_2. 1 hit.
PF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 2 hits.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53704-1 [UniParc]FASTAAdd to Basket

« Hide

MCGIFGYVNF LVDKSRGEII DNLIEGLQRL EYRGYDSAGI AVDGKLTKDP    50
SNGDEEYMDS IIVKTTGKVK VLKQKIIDDQ IDRSAIFDNH VGIAHTRWAT 100
HGQPKTENCH PHKSDPKGEF IVVHNGIITN YAALRKYLLS KGHVFESETD 150
TECIAKLFKH FYDLNVKAGV FPDLNELTKQ VLHELEGSYG LLVKSYHYPG 200
EVCGTRKGSP LLVGVKTDKK LKVDFVDVEF EAQQQHRPQQ PQINHNGATS 250
AAELGFIPVA PGEQNLRTSQ SRAFLSEDDL PMPVEFFLSS DPASVVQHTK 300
KVLFLEDDDI AHIYDGELRI HRASTKSAGE STVRPIQTLE MELNEIMKGP 350
YKHFMQKEIF EQPDSAFNTM RGRIDFENCV VTLGGLKSWL STIRRCRRII 400
MIACGTSYHS CLATRSIFEE LTEIPVSVEL ASDFLDRRSP VFRDDTCVFV 450
SQSGETADSI LALQYCLERG ALTVGIVNSV GSSMSRQTHC GVHINAGPEI 500
GVASTKAYTS QYIALVMFAL SLSNDSISRK GRHEEIIKGL QKIPEQIKQV 550
LKLENKIKDL CNSSLNDQKS LLLLGRGYQF ATALEGALKI KEISYMHSEG 600
VLAGELKHGI LALVDEDLPI IAFATRDSLF PKVMSAIEQV TARDGRPIVI 650
CNEGDAIISN DKVHTTLEVP ETVDCLQGLL NVIPLQLISY WLAVNRGIDV 700
DFPRNLAKSV TVE 713
Length:713
Mass (Da):79,268
Last modified:January 23, 2007 - v2
Checksum:i595EB8E90D704D53
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X94753 Genomic DNA. Translation: CAA64380.1.
AACQ01000013 Genomic DNA. Translation: EAL02907.1.
PIRiJC6012.
RefSeqiXP_721697.1. XM_716604.1.

Genome annotation databases

GeneIDi3636557.
KEGGical:CaO19.1618.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X94753 Genomic DNA. Translation: CAA64380.1 .
AACQ01000013 Genomic DNA. Translation: EAL02907.1 .
PIRi JC6012.
RefSeqi XP_721697.1. XM_716604.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2POC X-ray 1.80 A/B/C/D 347-713 [» ]
2PUT X-ray 1.90 A/B/C/D 347-713 [» ]
2PUV X-ray 1.90 A/B/C/D 347-713 [» ]
2PUW X-ray 3.15 A/B 347-713 [» ]
ProteinModelPortali P53704.
SMRi P53704. Positions 350-701.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5476.CAL0006344.

Chemistry

BindingDBi P53704.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3636557.
KEGGi cal:CaO19.1618.

Organism-specific databases

CGDi CAL0006344. GFA1.

Phylogenomic databases

eggNOGi COG0449.
KOi K00820.
OrthoDBi EOG7034RG.

Enzyme and pathway databases

UniPathwayi UPA00113 ; UER00528 .
BioCyci MetaCyc:MONOMER-13172.

Miscellaneous databases

EvolutionaryTracei P53704.

Family and domain databases

Gene3Di 3.60.20.10. 2 hits.
InterProi IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view ]
PANTHERi PTHR10937:SF0. PTHR10937:SF0. 1 hit.
Pfami PF00310. GATase_2. 1 hit.
PF01380. SIS. 2 hits.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 2 hits.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of the GFA1 gene encoding the glutamine:fructose-6-phosphate amidotransferase of Candida albicans."
    Smith R.J., Milewski S., Brown A.J., Gooday G.W.
    J. Bacteriol. 178:2320-2327(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 10261 / CBS 2718 / NBRC 1061.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SC5314 / ATCC MYA-2876.
  3. "The crystal and solution studies of glucosamine-6-phosphate synthase from Candida albicans."
    Raczynska J., Olchowy J., Konariev P.V., Svergun D.I., Milewski S., Rypniewski W.
    J. Mol. Biol. 372:672-688(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 347-713, SUBUNIT.

Entry informationi

Entry nameiGFA1_CANAL
AccessioniPrimary (citable) accession number: P53704
Secondary accession number(s): Q5AJV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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