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P53704

- GFA1_CANAL

UniProt

P53704 - GFA1_CANAL

Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

GFA1

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins).

    Catalytic activityi

    L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activityBy similarity

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: CGD

    GO - Biological processi

    1. cellular response to drug Source: CGD
    2. cell wall chitin biosynthetic process Source: CGD
    3. chitin biosynthetic process Source: CGD
    4. glucosamine biosynthetic process Source: CGD
    5. glutamine metabolic process Source: UniProtKB-KW
    6. hyphal growth Source: CGD
    7. protein N-linked glycosylation Source: CGD
    8. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13172.
    UniPathwayiUPA00113; UER00528.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
    Short name:
    GFAT
    Alternative name(s):
    D-fructose-6-phosphate amidotransferase
    Hexosephosphate aminotransferase
    Gene namesi
    Name:GFA1
    ORF Names:CaO19.1618
    OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
    Taxonomic identifieri237561 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida
    ProteomesiUP000000559: Unassembled WGS sequence

    Organism-specific databases

    CGDiCAL0006344. GFA1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 713712Glutamine--fructose-6-phosphate aminotransferase [isomerizing]PRO_0000135287Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi5476.CAL0006344.

    Structurei

    Secondary structure

    1
    713
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi354 – 3607
    Helixi362 – 3709
    Turni371 – 3733
    Turni376 – 3794
    Turni384 – 3863
    Helixi387 – 3893
    Helixi390 – 3945
    Beta strandi396 – 4038
    Helixi405 – 42218
    Beta strandi426 – 4305
    Helixi431 – 4366
    Beta strandi445 – 45410
    Helixi457 – 46812
    Beta strandi472 – 4798
    Helixi483 – 4875
    Beta strandi488 – 4936
    Beta strandi501 – 5033
    Helixi506 – 52217
    Turni523 – 5253
    Turni527 – 5293
    Helixi530 – 55021
    Helixi551 – 5533
    Helixi554 – 5629
    Helixi564 – 5674
    Beta strandi569 – 5757
    Helixi577 – 5793
    Helixi580 – 59415
    Beta strandi597 – 6026
    Beta strandi620 – 6234
    Helixi626 – 6283
    Helixi631 – 64212
    Beta strandi648 – 6525
    Beta strandi665 – 6695
    Helixi674 – 6763
    Helixi677 – 69519

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2POCX-ray1.80A/B/C/D347-713[»]
    2PUTX-ray1.90A/B/C/D347-713[»]
    2PUVX-ray1.90A/B/C/D347-713[»]
    2PUWX-ray3.15A/B347-713[»]
    ProteinModelPortaliP53704.
    SMRiP53704. Positions 350-701.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP53704.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 316315Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST
    Domaini389 – 528140SIS 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini561 – 703143SIS 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation
    Contains 2 SIS domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase, Repeat

    Phylogenomic databases

    eggNOGiCOG0449.
    KOiK00820.
    OrthoDBiEOG7034RG.

    Family and domain databases

    Gene3Di3.60.20.10. 2 hits.
    InterProiIPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR005855. GlmS_trans.
    IPR029055. Ntn_hydrolases_N.
    IPR001347. SIS.
    [Graphical view]
    PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
    PfamiPF00310. GATase_2. 1 hit.
    PF01380. SIS. 2 hits.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 2 hits.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS51464. SIS. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P53704-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCGIFGYVNF LVDKSRGEII DNLIEGLQRL EYRGYDSAGI AVDGKLTKDP    50
    SNGDEEYMDS IIVKTTGKVK VLKQKIIDDQ IDRSAIFDNH VGIAHTRWAT 100
    HGQPKTENCH PHKSDPKGEF IVVHNGIITN YAALRKYLLS KGHVFESETD 150
    TECIAKLFKH FYDLNVKAGV FPDLNELTKQ VLHELEGSYG LLVKSYHYPG 200
    EVCGTRKGSP LLVGVKTDKK LKVDFVDVEF EAQQQHRPQQ PQINHNGATS 250
    AAELGFIPVA PGEQNLRTSQ SRAFLSEDDL PMPVEFFLSS DPASVVQHTK 300
    KVLFLEDDDI AHIYDGELRI HRASTKSAGE STVRPIQTLE MELNEIMKGP 350
    YKHFMQKEIF EQPDSAFNTM RGRIDFENCV VTLGGLKSWL STIRRCRRII 400
    MIACGTSYHS CLATRSIFEE LTEIPVSVEL ASDFLDRRSP VFRDDTCVFV 450
    SQSGETADSI LALQYCLERG ALTVGIVNSV GSSMSRQTHC GVHINAGPEI 500
    GVASTKAYTS QYIALVMFAL SLSNDSISRK GRHEEIIKGL QKIPEQIKQV 550
    LKLENKIKDL CNSSLNDQKS LLLLGRGYQF ATALEGALKI KEISYMHSEG 600
    VLAGELKHGI LALVDEDLPI IAFATRDSLF PKVMSAIEQV TARDGRPIVI 650
    CNEGDAIISN DKVHTTLEVP ETVDCLQGLL NVIPLQLISY WLAVNRGIDV 700
    DFPRNLAKSV TVE 713
    Length:713
    Mass (Da):79,268
    Last modified:January 23, 2007 - v2
    Checksum:i595EB8E90D704D53
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X94753 Genomic DNA. Translation: CAA64380.1.
    AACQ01000013 Genomic DNA. Translation: EAL02907.1.
    PIRiJC6012.
    RefSeqiXP_721697.1. XM_716604.1.

    Genome annotation databases

    GeneIDi3636557.
    KEGGical:CaO19.1618.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X94753 Genomic DNA. Translation: CAA64380.1 .
    AACQ01000013 Genomic DNA. Translation: EAL02907.1 .
    PIRi JC6012.
    RefSeqi XP_721697.1. XM_716604.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2POC X-ray 1.80 A/B/C/D 347-713 [» ]
    2PUT X-ray 1.90 A/B/C/D 347-713 [» ]
    2PUV X-ray 1.90 A/B/C/D 347-713 [» ]
    2PUW X-ray 3.15 A/B 347-713 [» ]
    ProteinModelPortali P53704.
    SMRi P53704. Positions 350-701.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5476.CAL0006344.

    Chemistry

    BindingDBi P53704.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3636557.
    KEGGi cal:CaO19.1618.

    Organism-specific databases

    CGDi CAL0006344. GFA1.

    Phylogenomic databases

    eggNOGi COG0449.
    KOi K00820.
    OrthoDBi EOG7034RG.

    Enzyme and pathway databases

    UniPathwayi UPA00113 ; UER00528 .
    BioCyci MetaCyc:MONOMER-13172.

    Miscellaneous databases

    EvolutionaryTracei P53704.

    Family and domain databases

    Gene3Di 3.60.20.10. 2 hits.
    InterProi IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR005855. GlmS_trans.
    IPR029055. Ntn_hydrolases_N.
    IPR001347. SIS.
    [Graphical view ]
    PANTHERi PTHR10937:SF0. PTHR10937:SF0. 1 hit.
    Pfami PF00310. GATase_2. 1 hit.
    PF01380. SIS. 2 hits.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 2 hits.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS51464. SIS. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of the GFA1 gene encoding the glutamine:fructose-6-phosphate amidotransferase of Candida albicans."
      Smith R.J., Milewski S., Brown A.J., Gooday G.W.
      J. Bacteriol. 178:2320-2327(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 10261 / CBS 2718 / NBRC 1061.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SC5314 / ATCC MYA-2876.
    3. "The crystal and solution studies of glucosamine-6-phosphate synthase from Candida albicans."
      Raczynska J., Olchowy J., Konariev P.V., Svergun D.I., Milewski S., Rypniewski W.
      J. Mol. Biol. 372:672-688(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 347-713, SUBUNIT.

    Entry informationi

    Entry nameiGFA1_CANAL
    AccessioniPrimary (citable) accession number: P53704
    Secondary accession number(s): Q5AJV2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 92 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Candida albicans
      Candida albicans: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3