P53700 (CCHL_CANAW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c heme lyase
Short name=CCHL
EC=4.4.1.17

Alternative name(s):
Holocytochrome-c synthase

Gene names

Name:	CYC3
ORF Names:	CAWG_04502

Organism

Candida albicans (strain WO-1) (Yeast)

Taxonomic identifier

294748 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Links covalently the heme group to the apoprotein of cytochrome c.
Catalytic activity	Holocytochrome c = apocytochrome c + heme.
Subcellular location	Mitochondrion inner membrane.
Sequence similarities	Belongs to the cytochrome c-type heme lyase family. Contains 2 HRM (heme regulatory motif) repeats.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Repeat
Ligand	Heme Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	holocytochrome-c synthase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Cytochrome c heme lyase		PRO_0000121717
Regions
	Repeat	25 – 30	6	HRM 1
	Repeat	39 – 44	6	HRM 2
Experimental info
	Sequence conflict	114	1	G → V in AAB04135. Ref.1
	Sequence conflict	224	1	T → A in AAB04135. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P53700 [UniParc]. Last modified October 19, 2011. Version 2. Checksum: 89923AB2FC3A97A6	FASTA	264	30,170
	10 20 30 40 50 60 MGWFWADKPS QDTVKATSSF TTPSACPIDH SKLASSSPTC PVKLNNDNDE VLNPLNNMPM 70 80 90 100 110 120 AISSERAPGQ RIKLSTERTI STIPRGESED QGLWEYPSPQ QMLNAMLSKG KGDGVPEDAV 130 140 150 160 170 180 ESMVEVHNFL NEGAWQQILT WEDQYTQQTK VEPRLKKFTG RPHDLSPKAR MYLWLGQLFP 190 200 210 220 230 240 ETFNTIPPFD RHDWTVLRSC GRNQGWKEVR YVIDYYGAPD DEETGMPAFM LDTRPALDNL 250 260 TNARDRFTHW AYPLWKKAMG EVRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a Candida albicans gene coding for cytochrome c haem lyase and a cell wall-related protein." Cervera A.M., Gozalbo D., McCreath K.J., Gow N.A., Martinez J.P., Casanova M. Mol. Microbiol. 30:67-81(1998) [PubMed: 9786186] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: WO-1.
[2]	"Evolution of pathogenicity and sexual reproduction in eight Candida genomes." Butler G., Rasmussen M.D., Lin M.F., Santos M.A.S., Sakthikumar S., Munro C.A., Rheinbay E., Grabherr M., Forche A., Reedy J.L., Agrafioti I., Arnaud M.B., Bates S., Brown A.J.P., Brunke S., Costanzo M.C., Fitzpatrick D.A., de Groot P.W.J. , Harris D., Hoyer L.L., Hube B., Klis F.M., Kodira C., Lennard N., Logue M.E., Martin R., Neiman A.M., Nikolaou E., Quail M.A., Quinn J., Santos M.C., Schmitzberger F.F., Sherlock G., Shah P., Silverstein K.A.T., Skrzypek M.S., Soll D., Staggs R., Stansfield I., Stumpf M.P.H., Sudbery P.E., Srikantha T., Zeng Q., Berman J., Berriman M., Heitman J., Gow N.A.R., Lorenz M.C., Birren B.W., Kellis M., Cuomo C.A. Nature 459:657-662(2009) [PubMed: 19465905] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WO-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U62148 Genomic DNA. Translation: AAB04135.1. CM000311 Genomic DNA. Translation: EEQ46157.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P53700.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	4.4.1.17. 1096.
Family and domain databases
InterPro	IPR000511. Cyt_C/C1_haem_lyase. [Graphical view]
PANTHER	PTHR12743. Cyto_heme_lyase. 1 hit.
Pfam	PF01265. Cyto_heme_lyase. 1 hit. [Graphical view]
PROSITE	PS00821. CYTO_HEME_LYASE_1. 1 hit. PS00822. CYTO_HEME_LYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CCHL_CANAW

Accession

Primary (citable) accession number: P53700
Secondary accession number(s): C4YR04

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 19, 2011
Last modified:	December 14, 2011
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program