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Protein

NAD-dependent histone deacetylase HST4

Gene

HST4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NAD-dependent histone deacetylase, which contributes together with HST3 to histone H3 'Lys-56' deacetylation, regulation of telomeric silencing, proper cell cycle progression, DNA damage control, DNA recombination, and genomic maintenance.4 Publications

Catalytic activityi

NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.PROSITE-ProRule annotation

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei213Proton acceptorPROSITE-ProRule annotation1
Metal bindingi221ZincPROSITE-ProRule annotation1
Metal bindingi224ZincPROSITE-ProRule annotation1
Metal bindingi251ZincPROSITE-ProRule annotation1
Metal bindingi254ZincPROSITE-ProRule annotation1
Binding sitei363NAD; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi100 – 119NADBy similarityAdd BLAST20
Nucleotide bindingi184 – 187NADBy similarity4
Nucleotide bindingi310 – 312NADBy similarity3
Nucleotide bindingi340 – 342NADBy similarity3

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • NAD+ binding Source: InterPro
  • NAD-dependent histone deacetylase activity Source: SGD

GO - Biological processi

  • cellular response to biotin starvation Source: SGD
  • chromatin silencing at telomere Source: SGD
  • histone deacetylation Source: SGD
  • short-chain fatty acid metabolic process Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29779-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD-dependent histone deacetylase HST4 (EC:3.5.1.-)
Alternative name(s):
Homologous to SIR2 protein 4
Regulatory protein SIR2 homolog 4
Gene namesi
Name:HST4
Ordered Locus Names:YDR191W
ORF Names:YD9346.03
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR191W.
SGDiS000002599. HST4.

Subcellular locationi

GO - Cellular componenti

  • chromosome, telomeric region Source: GOC
  • cytoplasm Source: SGD
  • mitochondrion Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001102841 – 370NAD-dependent histone deacetylase HST4Add BLAST370

Proteomic databases

PRIDEiP53688.

Interactioni

Protein-protein interaction databases

BioGridi32244. 68 interactors.
IntActiP53688. 4 interactors.
MINTiMINT-4481318.

Structurei

3D structure databases

ProteinModelPortaliP53688.
SMRiP53688.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini83 – 370Deacetylase sirtuin-typePROSITE-ProRule annotationAdd BLAST288

Sequence similaritiesi

Belongs to the sirtuin family. Class I subfamily.Curated
Contains 1 deacetylase sirtuin-type domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000075988.
HOGENOMiHOG000111204.
InParanoidiP53688.
KOiK11121.
OMAiQLHGSIN.
OrthoDBiEOG092C4DBJ.

Family and domain databases

Gene3Di3.30.1600.10. 2 hits.
3.40.50.1220. 3 hits.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR026591. Sirtuin_cat_small_dom.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERiPTHR11085. PTHR11085. 2 hits.
PfamiPF02146. SIR2. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiPS50305. SIRTUIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53688-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQKFVLPIT PPSTAEKKPQ TENRCNENLK PRRLLPQLKK SVRNRKPRLS
60 70 80 90 100
YRPELNSVFD LDAYVDSTHL SKSQRHHMDR DAGFISYALN YSKRMVVVSG
110 120 130 140 150
AGISVAAGIP DFRSSEGIFS TVNGGSGKDL FDYNRVYGDE SMSLKFNQLM
160 170 180 190 200
VSLFRLSKNC QPTKFHEMLN EFARDGRLLR LYTQNIDGLD TQLPHLSTNV
210 220 230 240 250
PLAKPIPSTV QLHGSIKHME CNKCLNIKPF DPELFKCDDK FDSRTEIIPS
260 270 280 290 300
CPQCEEYETV RKMAGLRSTG VGKLRPRVIL YNEVHPEGDF IGEIANNDLK
310 320 330 340 350
KRIDCLIIVG TSLKIPGVKN ICRQFAAKVH ANRGIVLYLN TSMPPKNVLD
360 370
SLKFVDLVVL GDCQHVTSLL
Length:370
Mass (Da):41,765
Last modified:October 1, 1996 - v1
Checksum:i86BB0238BFA914F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48784 Genomic DNA. Translation: CAA88705.1.
AY557699 Genomic DNA. Translation: AAS56025.1.
BK006938 Genomic DNA. Translation: DAA12034.1.
PIRiS52699.
RefSeqiNP_010477.3. NM_001180499.3.

Genome annotation databases

EnsemblFungiiYDR191W; YDR191W; YDR191W.
GeneIDi851772.
KEGGisce:YDR191W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48784 Genomic DNA. Translation: CAA88705.1.
AY557699 Genomic DNA. Translation: AAS56025.1.
BK006938 Genomic DNA. Translation: DAA12034.1.
PIRiS52699.
RefSeqiNP_010477.3. NM_001180499.3.

3D structure databases

ProteinModelPortaliP53688.
SMRiP53688.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32244. 68 interactors.
IntActiP53688. 4 interactors.
MINTiMINT-4481318.

Proteomic databases

PRIDEiP53688.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR191W; YDR191W; YDR191W.
GeneIDi851772.
KEGGisce:YDR191W.

Organism-specific databases

EuPathDBiFungiDB:YDR191W.
SGDiS000002599. HST4.

Phylogenomic databases

GeneTreeiENSGT00550000075988.
HOGENOMiHOG000111204.
InParanoidiP53688.
KOiK11121.
OMAiQLHGSIN.
OrthoDBiEOG092C4DBJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-29779-MONOMER.

Miscellaneous databases

PROiP53688.

Family and domain databases

Gene3Di3.30.1600.10. 2 hits.
3.40.50.1220. 3 hits.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR026591. Sirtuin_cat_small_dom.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERiPTHR11085. PTHR11085. 2 hits.
PfamiPF02146. SIR2. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiPS50305. SIRTUIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHST4_YEAST
AccessioniPrimary (citable) accession number: P53688
Secondary accession number(s): D6VSH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.