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Protein

NAD-dependent histone deacetylase HST4

Gene

HST4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NAD-dependent histone deacetylase, which contributes together with HST3 to histone H3 'Lys-56' deacetylation, regulation of telomeric silencing, proper cell cycle progression, DNA damage control, DNA recombination, and genomic maintenance.4 Publications

Catalytic activityi

NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.PROSITE-ProRule annotation

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei213 – 2131Proton acceptorPROSITE-ProRule annotation
Metal bindingi221 – 2211ZincPROSITE-ProRule annotation
Metal bindingi224 – 2241ZincPROSITE-ProRule annotation
Metal bindingi251 – 2511ZincPROSITE-ProRule annotation
Metal bindingi254 – 2541ZincPROSITE-ProRule annotation
Binding sitei363 – 3631NAD; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi100 – 11920NADBy similarityAdd
BLAST
Nucleotide bindingi184 – 1874NADBy similarity
Nucleotide bindingi310 – 3123NADBy similarity
Nucleotide bindingi340 – 3423NADBy similarity

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • NAD+ binding Source: InterPro
  • NAD-dependent histone deacetylase activity Source: SGD

GO - Biological processi

  • cellular response to biotin starvation Source: SGD
  • chromatin silencing at telomere Source: SGD
  • histone deacetylation Source: SGD
  • short-chain fatty acid metabolic process Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29779-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD-dependent histone deacetylase HST4 (EC:3.5.1.-)
Alternative name(s):
Homologous to SIR2 protein 4
Regulatory protein SIR2 homolog 4
Gene namesi
Name:HST4
Ordered Locus Names:YDR191W
ORF Names:YD9346.03
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR191W.
SGDiS000002599. HST4.

Subcellular locationi

GO - Cellular componenti

  • chromosome, telomeric region Source: GOC
  • cytoplasm Source: SGD
  • mitochondrion Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370NAD-dependent histone deacetylase HST4PRO_0000110284Add
BLAST

Interactioni

Protein-protein interaction databases

BioGridi32244. 68 interactions.
IntActiP53688. 4 interactions.
MINTiMINT-4481318.

Structurei

3D structure databases

ProteinModelPortaliP53688.
SMRiP53688. Positions 89-329.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini83 – 370288Deacetylase sirtuin-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the sirtuin family. Class I subfamily.Curated
Contains 1 deacetylase sirtuin-type domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000075988.
HOGENOMiHOG000111204.
InParanoidiP53688.
KOiK11121.
OMAiENRCNEN.
OrthoDBiEOG74J9KM.

Family and domain databases

Gene3Di3.30.1600.10. 2 hits.
3.40.50.1220. 3 hits.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR026591. Sirtuin_cat_small_dom.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERiPTHR11085. PTHR11085. 2 hits.
PfamiPF02146. SIR2. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiPS50305. SIRTUIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53688-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQKFVLPIT PPSTAEKKPQ TENRCNENLK PRRLLPQLKK SVRNRKPRLS
60 70 80 90 100
YRPELNSVFD LDAYVDSTHL SKSQRHHMDR DAGFISYALN YSKRMVVVSG
110 120 130 140 150
AGISVAAGIP DFRSSEGIFS TVNGGSGKDL FDYNRVYGDE SMSLKFNQLM
160 170 180 190 200
VSLFRLSKNC QPTKFHEMLN EFARDGRLLR LYTQNIDGLD TQLPHLSTNV
210 220 230 240 250
PLAKPIPSTV QLHGSIKHME CNKCLNIKPF DPELFKCDDK FDSRTEIIPS
260 270 280 290 300
CPQCEEYETV RKMAGLRSTG VGKLRPRVIL YNEVHPEGDF IGEIANNDLK
310 320 330 340 350
KRIDCLIIVG TSLKIPGVKN ICRQFAAKVH ANRGIVLYLN TSMPPKNVLD
360 370
SLKFVDLVVL GDCQHVTSLL
Length:370
Mass (Da):41,765
Last modified:October 1, 1996 - v1
Checksum:i86BB0238BFA914F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48784 Genomic DNA. Translation: CAA88705.1.
AY557699 Genomic DNA. Translation: AAS56025.1.
BK006938 Genomic DNA. Translation: DAA12034.1.
PIRiS52699.
RefSeqiNP_010477.3. NM_001180499.3.

Genome annotation databases

EnsemblFungiiYDR191W; YDR191W; YDR191W.
GeneIDi851772.
KEGGisce:YDR191W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48784 Genomic DNA. Translation: CAA88705.1.
AY557699 Genomic DNA. Translation: AAS56025.1.
BK006938 Genomic DNA. Translation: DAA12034.1.
PIRiS52699.
RefSeqiNP_010477.3. NM_001180499.3.

3D structure databases

ProteinModelPortaliP53688.
SMRiP53688. Positions 89-329.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32244. 68 interactions.
IntActiP53688. 4 interactions.
MINTiMINT-4481318.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR191W; YDR191W; YDR191W.
GeneIDi851772.
KEGGisce:YDR191W.

Organism-specific databases

EuPathDBiFungiDB:YDR191W.
SGDiS000002599. HST4.

Phylogenomic databases

GeneTreeiENSGT00550000075988.
HOGENOMiHOG000111204.
InParanoidiP53688.
KOiK11121.
OMAiENRCNEN.
OrthoDBiEOG74J9KM.

Enzyme and pathway databases

BioCyciYEAST:G3O-29779-MONOMER.

Miscellaneous databases

NextBioi969567.
PROiP53688.

Family and domain databases

Gene3Di3.30.1600.10. 2 hits.
3.40.50.1220. 3 hits.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR026591. Sirtuin_cat_small_dom.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERiPTHR11085. PTHR11085. 2 hits.
PfamiPF02146. SIR2. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiPS50305. SIRTUIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "HST1, a new member of the SIR2 family of genes."
    Derbyshire M.K., Weinstock K.G., Strathern J.N.
    Yeast 12:631-640(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME.
    Strain: GRY 668.
  5. "The SIR2 gene family, conserved from bacteria to humans, functions in silencing, cell cycle progression, and chromosome stability."
    Brachmann C.B., Sherman J.M., Devine S.E., Cameron E.E., Pillus L., Boeke J.D.
    Genes Dev. 9:2888-2902(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. Cited for: FUNCTION.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "A DNA integrity network in the yeast Saccharomyces cerevisiae."
    Pan X., Ye P., Yuan D.S., Wang X., Bader J.S., Boeke J.D.
    Cell 124:1069-1081(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Taking it off: regulation of H3 K56 acetylation by Hst3 and Hst4."
    Miller K.M., Maas N.L., Toczyski D.P.
    Cell Cycle 5:2561-2565(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  11. "The sirtuins Hst3 and Hst4p preserve genome integrity by controlling histone H3 lysine 56 deacetylation."
    Celic I., Masumoto H., Griffith W.P., Meluh P., Cotter R.J., Boeke J.D., Verreault A.
    Curr. Biol. 16:1280-1289(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiHST4_YEAST
AccessioniPrimary (citable) accession number: P53688
Secondary accession number(s): D6VSH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.