Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NAD-dependent histone deacetylase HST3

Gene

HST3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NAD-dependent histone deacetylase, which contributes together with HST4 to histone H3 'Lys-56' deacetylation, regulation of telomeric silencing, proper cell cycle progression, DNA damage control, DNA recombination, and genomic maintenance.4 Publications

Catalytic activityi

NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.PROSITE-ProRule annotation

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei187 – 1871Proton acceptorPROSITE-ProRule annotation
Metal bindingi195 – 1951ZincPROSITE-ProRule annotation
Metal bindingi198 – 1981ZincPROSITE-ProRule annotation
Metal bindingi220 – 2201ZincPROSITE-ProRule annotation
Metal bindingi223 – 2231ZincPROSITE-ProRule annotation
Binding sitei333 – 3331NAD; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi60 – 7920NADBy similarityAdd
BLAST
Nucleotide bindingi151 – 1544NADBy similarity
Nucleotide bindingi282 – 2843NADBy similarity
Nucleotide bindingi312 – 3143NADBy similarity

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • NAD+ binding Source: InterPro
  • NAD-dependent histone deacetylase activity Source: SGD

GO - Biological processi

  • chromatin silencing at telomere Source: SGD
  • histone deacetylation Source: SGD
  • short-chain fatty acid metabolic process Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33572-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD-dependent histone deacetylase HST3 (EC:3.5.1.-)
Alternative name(s):
Homologous to SIR2 protein 3
Regulatory protein SIR2 homolog 3
Gene namesi
Name:HST3
Ordered Locus Names:YOR025W
ORF Names:OR26.15
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR025W.
SGDiS000005551. HST3.

Subcellular locationi

GO - Cellular componenti

  • chromosome, telomeric region Source: GOC
  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 447447NAD-dependent histone deacetylase HST3PRO_0000110283Add
BLAST

PTM databases

iPTMnetiP53687.

Interactioni

Protein-protein interaction databases

BioGridi34428. 172 interactions.
DIPiDIP-1378N.
IntActiP53687. 3 interactions.
MINTiMINT-410731.

Structurei

3D structure databases

ProteinModelPortaliP53687.
SMRiP53687. Positions 48-319.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 349307Deacetylase sirtuin-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the sirtuin family. Class I subfamily.Curated
Contains 1 deacetylase sirtuin-type domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000075988.
HOGENOMiHOG000094160.
InParanoidiP53687.
KOiK11121.
OMAiHRFIKIL.
OrthoDBiEOG7NW6MC.

Family and domain databases

Gene3Di3.30.1600.10. 2 hits.
3.40.50.1220. 3 hits.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR026591. Sirtuin_cat_small_dom.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERiPTHR11085. PTHR11085. 3 hits.
PfamiPF02146. SIR2. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiPS50305. SIRTUIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53687-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSVSPSPPA SRSGSMCSDL PSSLQTEKLA HIIGLDADDE VLRRVTKQLS
60 70 80 90 100
RSRRIACLTG AGISCNAGIP DFRSSDGLYD LVKKDCSQYW SIKSGREMFD
110 120 130 140 150
ISLFRDDFKI SIFAKFMERL YSNVQLAKPT KTHKFIAHLK DRNKLLRCYT
160 170 180 190 200
QNIDGLEESI GLTLSNRKLP LTSFSSHWKN LDVVQLHGDL KTLSCTKCFQ
210 220 230 240 250
TFPWSRYWSR CLRRGELPLC PDCEALINKR LNEGKRTLGS NVGILRPNIV
260 270 280 290 300
LYGENHPSCE IITQGLNLDI IKGNPDFLII MGTSLKVDGV KQLVKKLSKK
310 320 330 340 350
IHDRGGLIIL VNKTPIGESS WHGIIDYQIH SDCDNWVTFL ESQIPDFFKT
360 370 380 390 400
QDQIKKLRQL KREASDLRKQ MKAQKDSIGT PPTTPLRTAQ GIDIQGNNEL
410 420 430 440
NTKIKSLNTV KRKILSPENS SEEDEEENLD TRKRAKIRPT FGDNQAS
Length:447
Mass (Da):50,524
Last modified:October 1, 1996 - v1
Checksum:i59CD5FBD712B7005
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti418 – 4181E → G in AAU09783 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39062 Genomic DNA. Translation: AAA81034.1.
X87331 Genomic DNA. Translation: CAA60741.1.
Z74933 Genomic DNA. Translation: CAA99215.1.
AY723866 Genomic DNA. Translation: AAU09783.1.
BK006948 Genomic DNA. Translation: DAA10807.1.
PIRiS54631.
RefSeqiNP_014668.1. NM_001183444.1.

Genome annotation databases

EnsemblFungiiYOR025W; YOR025W; YOR025W.
GeneIDi854190.
KEGGisce:YOR025W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39062 Genomic DNA. Translation: AAA81034.1.
X87331 Genomic DNA. Translation: CAA60741.1.
Z74933 Genomic DNA. Translation: CAA99215.1.
AY723866 Genomic DNA. Translation: AAU09783.1.
BK006948 Genomic DNA. Translation: DAA10807.1.
PIRiS54631.
RefSeqiNP_014668.1. NM_001183444.1.

3D structure databases

ProteinModelPortaliP53687.
SMRiP53687. Positions 48-319.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34428. 172 interactions.
DIPiDIP-1378N.
IntActiP53687. 3 interactions.
MINTiMINT-410731.

PTM databases

iPTMnetiP53687.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR025W; YOR025W; YOR025W.
GeneIDi854190.
KEGGisce:YOR025W.

Organism-specific databases

EuPathDBiFungiDB:YOR025W.
SGDiS000005551. HST3.

Phylogenomic databases

GeneTreeiENSGT00550000075988.
HOGENOMiHOG000094160.
InParanoidiP53687.
KOiK11121.
OMAiHRFIKIL.
OrthoDBiEOG7NW6MC.

Enzyme and pathway databases

BioCyciYEAST:G3O-33572-MONOMER.

Miscellaneous databases

PROiP53687.

Family and domain databases

Gene3Di3.30.1600.10. 2 hits.
3.40.50.1220. 3 hits.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR026591. Sirtuin_cat_small_dom.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERiPTHR11085. PTHR11085. 3 hits.
PfamiPF02146. SIR2. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiPS50305. SIRTUIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The SIR2 gene family, conserved from bacteria to humans, functions in silencing, cell cycle progression, and chromosome stability."
    Brachmann C.B., Sherman J.M., Devine S.E., Cameron E.E., Pillus L., Boeke J.D.
    Genes Dev. 9:2888-2902(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / YPH1.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: FUNCTION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A DNA integrity network in the yeast Saccharomyces cerevisiae."
    Pan X., Ye P., Yuan D.S., Wang X., Bader J.S., Boeke J.D.
    Cell 124:1069-1081(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Taking it off: regulation of H3 K56 acetylation by Hst3 and Hst4."
    Miller K.M., Maas N.L., Toczyski D.P.
    Cell Cycle 5:2561-2565(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  10. "The sirtuins Hst3 and Hst4p preserve genome integrity by controlling histone H3 lysine 56 deacetylation."
    Celic I., Masumoto H., Griffith W.P., Meluh P., Cotter R.J., Boeke J.D., Verreault A.
    Curr. Biol. 16:1280-1289(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiHST3_YEAST
AccessioniPrimary (citable) accession number: P53687
Secondary accession number(s): D6W291, Q66R16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 319 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.