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Protein

Calcium-dependent protein kinase 23

Gene

CPK23

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger (By similarity). Involved in the activation of sucrose synthase 2 (SUS2) in developing seeds. Phosphorylates SUS2 at Ser-10 in a calcium-dependent manner. Phosphorylation of Ser-10 leads to the activation of SUS2, which supplies substrates for the biosynthesis of storage products in immature seeds (PubMed:11910009).By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei102ATPPROSITE-ProRule annotation1
Active sitei197Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi79 – 87ATPPROSITE-ProRule annotation9
Calcium bindingi387 – 3981PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi422 – 4322; atypicalPROSITE-ProRule annotationAdd BLAST11
Calcium bindingi457 – 4683PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi491 – 5024PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 4460.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 23Curated (EC:2.7.11.11 Publication)
Short name:
OsCDPK23Curated
Short name:
OsCPK231 Publication
Alternative name(s):
Calcium-dependent protein kinase isoform 1Curated
Short name:
CDPK1Curated
Seed-specific protein kinaseCurated
Gene namesi
Name:CPK231 Publication
Synonyms:SPK1 Publication
Ordered Locus Names:Os10g0539600Imported, LOC_Os10g39420Imported
ORF Names:OSJNBb0064P21.2Imported
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 10

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00000858282 – 534Calcium-dependent protein kinase 23Add BLAST533

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiP53682.
PRIDEiP53682.

Expressioni

Developmental stagei

Specifically expressed in endosperm of developing seeds.1 Publication

Inductioni

By UV-C.1 Publication

Gene expression databases

GenevisibleiP53682. OS.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi39947.LOC_Os10g39420.1.

Structurei

3D structure databases

ProteinModelPortaliP53682.
SMRiP53682.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini73 – 331Protein kinasePROSITE-ProRule annotationAdd BLAST259
Domaini374 – 408EF-hand 1PROSITE-ProRule annotationAdd BLAST35
Domaini409 – 443EF-hand 2; degeneratePROSITE-ProRule annotationAdd BLAST35
Domaini444 – 479EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini480 – 513EF-hand 4PROSITE-ProRule annotationAdd BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni337 – 367Autoinhibitory domainBy similarityAdd BLAST31

Domaini

There are 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (337-367) inactivates kinase activity under calcium-free conditions.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiP53682.
KOiK13412.
OMAiPWICDNG.
OrthoDBiEOG0936055E.

Family and domain databases

CDDicd00051. EFh. 1 hit.
InterProiView protein in InterPro
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF13499. EF-hand_7. 1 hit.
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00054. EFh. 2 hits.
SM00220. S_TKc. 1 hit.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53682-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNSCQNGTY GNNYQNSNRF QNDRFASRYV DGNDTEDCYS GSSRASLAGA
60 70 80 90 100
LRQGLNLKSP VLGYKTPNVR ELYTLGRELG QGQFGKTYLC TEISTGCQYA
110 120 130 140 150
CKTILKSNLR CVSDIEDVRR EIQIMHHLSG QKNIVTIKDT YEDEQAVHIV
160 170 180 190 200
MELCAGGELF SKIQKRGHYS ERKAAELIKI IVGIIETCHS HGVMHRDLKP
210 220 230 240 250
ENFLLLDADD EFSVKAIDFG LSVFFRPGQV FREVVGSPYY IAPEVLEKRY
260 270 280 290 300
GPEADIWTAG VILYVLLTGV PPFWADTQSG IYEKVLDGRI DFKSNRWPRI
310 320 330 340 350
SDSAKDLIKK MLCPYPSERL KAHEVLKHPW ICDNGVATNR ALDPSVLPRL
360 370 380 390 400
KQFSAMNRLK KLSLQIIAER LSEEEIVGLR EMFKAMDTKN RSVVTFGELK
410 420 430 440 450
GLKRYSSVFK DTEINDLMEA ADDTTSTINW EEFIAAAVSL NKIEREKHLM
460 470 480 490 500
AAFTYFDKDG SGFITVDKLQ KACMERNMED TFLEEMILEV DQNNDGQIDY
510 520 530
AEFVTMMQSN NFGLGWQTVE SSLNVALREA PQVY
Length:534
Mass (Da):60,547
Last modified:November 22, 2005 - v2
Checksum:i4217A06AE1990030
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti317S → L in BAA02698 (PubMed:8325505).Curated1
Sequence conflicti317S → L in ABL74562 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13436 mRNA. Translation: BAA02698.1.
EF122475 mRNA. Translation: ABL74562.1.
AC073166 Genomic DNA. Translation: AAG46110.1.
DP000086 Genomic DNA. Translation: AAP54840.1.
AP008216 Genomic DNA. Translation: BAF27094.1.
AP014966 Genomic DNA. Translation: BAT11850.1.
AK065456 mRNA. Translation: BAG89525.1.
PIRiJC1515.
RefSeqiXP_015614491.1. XM_015759005.1.
UniGeneiOs.8477.

Genome annotation databases

EnsemblPlantsiOS10T0539600-01; OS10T0539600-01; OS10G0539600.
GeneIDi4349249.
GrameneiOS10T0539600-01; OS10T0539600-01; OS10G0539600.
KEGGiosa:4349249.

Similar proteinsi

Entry informationi

Entry nameiCDPKN_ORYSJ
AccessioniPrimary (citable) accession number: P53682
Secondary accession number(s): A1YQI2
, B7EAX8, Q0IW21, Q7XCN1, Q9FRK2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 22, 2005
Last modified: August 30, 2017
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families