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Protein

AP-3 complex subunit mu-2

Gene

AP3M2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

SignaLinkiP53677.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-3 complex subunit mu-2
Alternative name(s):
Adaptor-related protein complex 3 subunit mu-2
Clathrin assembly protein assembly protein complex 3 mu-2 medium chain
Clathrin coat assembly protein AP47 homolog 2
Clathrin coat-associated protein AP47 homolog 2
Golgi adaptor AP-1 47 kDa protein homolog 2
HA1 47 kDa subunit homolog 2
Mu3B-adaptin
P47B
Gene namesi
Name:AP3M2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:570. AP3M2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24861.

Polymorphism and mutation databases

BioMutaiAP3M2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 418418AP-3 complex subunit mu-2PRO_0000193784Add
BLAST

Proteomic databases

EPDiP53677.
MaxQBiP53677.
PaxDbiP53677.
PRIDEiP53677.

2D gel databases

REPRODUCTION-2DPAGEP53677.

PTM databases

iPTMnetiP53677.
PhosphoSiteiP53677.

Expressioni

Gene expression databases

BgeeiP53677.
CleanExiHS_AP3M2.
ExpressionAtlasiP53677. baseline and differential.
GenevisibleiP53677. HS.

Organism-specific databases

HPAiCAB014876.

Interactioni

Subunit structurei

AP-3 associates with the BLOC-1 complex (By similarity). Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2).By similarity

Protein-protein interaction databases

BioGridi116147. 11 interactions.
IntActiP53677. 1 interaction.
STRINGi9606.ENSP00000174653.

Structurei

3D structure databases

ProteinModelPortaliP53677.
SMRiP53677. Positions 4-418.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini176 – 417242MHDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 MHD (mu homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2740. Eukaryota.
ENOG410XT7B. LUCA.
GeneTreeiENSGT00530000062779.
HOGENOMiHOG000234366.
HOVERGENiHBG104910.
InParanoidiP53677.
KOiK12398.
OMAiSGDVFME.
OrthoDBiEOG79KPF6.
PhylomeDBiP53677.
TreeFamiTF315187.

Family and domain databases

InterProiIPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view]
PfamiPF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
PRINTSiPR00314. CLATHRINADPT.
SUPFAMiSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P53677-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIHSLFLINS SGDIFLEKHW KSVVSRSVCD YFFEAQERAT EAENVPPVIP
60 70 80 90 100
TPHHYLLSVY RHKIFFVAVI QTEVPPLFVI EFLHRVVDTF QDYFGVCSEP
110 120 130 140 150
VIKDNVVVVY EVLEEMLDNG FPLATESNIL KELIKPPTIL RTVVNTITGS
160 170 180 190 200
TNVGDQLPTG QLSVVPWRRT GVKYTNNEAY FDVIEEIDAI IDKSGSTITA
210 220 230 240 250
EIQGVIDACV KLTGMPDLTL SFMNPRLLDD VSFHPCVRFK RWESERILSF
260 270 280 290 300
IPPDGNFRLL SYHVSAQNLV AIPVYVKHNI SFRDSSSLGR FEITVGPKQT
310 320 330 340 350
MGKTIEGVTV TSQMPKGVLN MSLTPSQGTH TFDPVTKMLS WDVGKINPQK
360 370 380 390 400
LPSLKGTMSL QAGASKPDEN PTINLQFKIQ QLAISGLKVN RLDMYGEKYK
410
PFKGIKYMTK AGKFQVRT
Length:418
Mass (Da):46,977
Last modified:October 1, 1996 - v1
Checksum:i8FCC8BB10505A7D5
GO
Isoform 2 (identifier: P53677-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     268-273: NLVAIP → KCCLGM
     274-418: Missing.

Note: No experimental confirmation available.
Show »
Length:273
Mass (Da):30,957
Checksum:i824A4F48383DBBA0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei268 – 2736NLVAIP → KCCLGM in isoform 2. 1 PublicationVSP_055790
Alternative sequencei274 – 418145Missing in isoform 2. 1 PublicationVSP_055791Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38293 mRNA. Translation: BAA07415.1.
AK315228 mRNA. Translation: BAG37657.1.
AC103724 Genomic DNA. No translation available.
CH471080 Genomic DNA. Translation: EAW63236.1.
CH471080 Genomic DNA. Translation: EAW63237.1.
CH471080 Genomic DNA. Translation: EAW63238.1.
BC056257 mRNA. Translation: AAH56257.1.
BC056398 mRNA. Translation: AAH56398.1.
CCDSiCCDS6125.1. [P53677-1]
PIRiA57170.
RefSeqiNP_001127768.1. NM_001134296.1. [P53677-1]
NP_006794.1. NM_006803.3. [P53677-1]
UniGeneiHs.654529.

Genome annotation databases

EnsembliENST00000174653; ENSP00000174653; ENSG00000070718. [P53677-1]
ENST00000396926; ENSP00000380132; ENSG00000070718. [P53677-1]
ENST00000518421; ENSP00000428787; ENSG00000070718. [P53677-1]
ENST00000530375; ENSP00000431918; ENSG00000070718. [P53677-2]
GeneIDi10947.
KEGGihsa:10947.
UCSCiuc003xoo.4. human. [P53677-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38293 mRNA. Translation: BAA07415.1.
AK315228 mRNA. Translation: BAG37657.1.
AC103724 Genomic DNA. No translation available.
CH471080 Genomic DNA. Translation: EAW63236.1.
CH471080 Genomic DNA. Translation: EAW63237.1.
CH471080 Genomic DNA. Translation: EAW63238.1.
BC056257 mRNA. Translation: AAH56257.1.
BC056398 mRNA. Translation: AAH56398.1.
CCDSiCCDS6125.1. [P53677-1]
PIRiA57170.
RefSeqiNP_001127768.1. NM_001134296.1. [P53677-1]
NP_006794.1. NM_006803.3. [P53677-1]
UniGeneiHs.654529.

3D structure databases

ProteinModelPortaliP53677.
SMRiP53677. Positions 4-418.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116147. 11 interactions.
IntActiP53677. 1 interaction.
STRINGi9606.ENSP00000174653.

PTM databases

iPTMnetiP53677.
PhosphoSiteiP53677.

Polymorphism and mutation databases

BioMutaiAP3M2.

2D gel databases

REPRODUCTION-2DPAGEP53677.

Proteomic databases

EPDiP53677.
MaxQBiP53677.
PaxDbiP53677.
PRIDEiP53677.

Protocols and materials databases

DNASUi10947.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000174653; ENSP00000174653; ENSG00000070718. [P53677-1]
ENST00000396926; ENSP00000380132; ENSG00000070718. [P53677-1]
ENST00000518421; ENSP00000428787; ENSG00000070718. [P53677-1]
ENST00000530375; ENSP00000431918; ENSG00000070718. [P53677-2]
GeneIDi10947.
KEGGihsa:10947.
UCSCiuc003xoo.4. human. [P53677-1]

Organism-specific databases

CTDi10947.
GeneCardsiAP3M2.
HGNCiHGNC:570. AP3M2.
HPAiCAB014876.
MIMi610469. gene.
neXtProtiNX_P53677.
PharmGKBiPA24861.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2740. Eukaryota.
ENOG410XT7B. LUCA.
GeneTreeiENSGT00530000062779.
HOGENOMiHOG000234366.
HOVERGENiHBG104910.
InParanoidiP53677.
KOiK12398.
OMAiSGDVFME.
OrthoDBiEOG79KPF6.
PhylomeDBiP53677.
TreeFamiTF315187.

Enzyme and pathway databases

SignaLinkiP53677.

Miscellaneous databases

GenomeRNAii10947.
NextBioi41593.
PROiP53677.
SOURCEiSearch...

Gene expression databases

BgeeiP53677.
CleanExiHS_AP3M2.
ExpressionAtlasiP53677. baseline and differential.
GenevisibleiP53677. HS.

Family and domain databases

InterProiIPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view]
PfamiPF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
PRINTSiPR00314. CLATHRINADPT.
SUPFAMiSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of 115 human chromosome 8-specific expressed-sequence tags by exon amplification."
    Koyama K., Sudo K., Nakamura Y.
    Genomics 26:245-253(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Testis.
  3. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain and Lung.

Entry informationi

Entry nameiAP3M2_HUMAN
AccessioniPrimary (citable) accession number: P53677
Secondary accession number(s): B2RCR0, D3DSY2, Q7Z472
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.