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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Mycoplasma pulmonis (strain UAB CTIP)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei255 – 2551ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:MYPU_6820
OrganismiMycoplasma pulmonis (strain UAB CTIP)
Taxonomic identifieri272635 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000528 Componenti: Chromosome

Organism-specific databases

GenoListiMYPU_6820.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 468468Glutamate--tRNA ligasePRO_0000119609Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272635.MYPU_6820.

Structurei

3D structure databases

ProteinModelPortaliP53662.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi10 – 2011"HIGH" regionAdd
BLAST
Motifi252 – 2565"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiAFRCFCT.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53662-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLRTRYAP SPTGYLHIGG ARTALFNYLL AKHYNGDFII RIEDTDVKRN
60 70 80 90 100
IADGEASQIE NLKWLNIEAN ESPLKPNEKY GPYRQSQKLE KYLKIAHELI
110 120 130 140 150
EKGYAYKAYD NSEELEEQKK HSEKLGVASF RYQRDFLKIS EEEKQKRDAS
160 170 180 190 200
GAYSIRVICP KNTTYQWDDL VRGNIAVNSN DIGDWIIIKS DDYPTYNFAV
210 220 230 240 250
VIDDIDMEIS HILRGEEHIT NTPKQMMIYD YLNAPKPLFG HLTIITNMEG
260 270 280 290 300
KKLSKRDLSL KQFIHEYKEE GYNSQAIFNF LTLLGWTDEK ARELMDHDEI
310 320 330 340 350
IKSFLYTRLS KSPSKFDITK MQWFSKQYWK NTPNEELIKI LNLNDYDNDW
360 370 380 390 400
INLFLDLYKE NIYSLNQLKN YLKIYKQANL NQEKDLDLND AEKNVVKSFS
410 420 430 440 450
SYIDYSNFSV NQIQEAINKT QEKLSIKGKN LFLPIRKATT FQEHGPELAK
460
AIYLFGSEII EKRMKKWK
Length:468
Mass (Da):55,066
Last modified:October 1, 1996 - v1
Checksum:i73026ACA2ED2941B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25415 Genomic DNA. Translation: AAA65629.1.
AL445565 Genomic DNA. Translation: CAC13855.1.
PIRiS49391.
RefSeqiNP_326513.1. NC_002771.1.
WP_010925483.1. NC_002771.1.

Genome annotation databases

EnsemblBacteriaiCAC13855; CAC13855; CAC13855.
KEGGimpu:MYPU_6820.
PATRICi20024318. VBIMycPul29704_0695.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25415 Genomic DNA. Translation: AAA65629.1.
AL445565 Genomic DNA. Translation: CAC13855.1.
PIRiS49391.
RefSeqiNP_326513.1. NC_002771.1.
WP_010925483.1. NC_002771.1.

3D structure databases

ProteinModelPortaliP53662.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272635.MYPU_6820.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC13855; CAC13855; CAC13855.
KEGGimpu:MYPU_6820.
PATRICi20024318. VBIMycPul29704_0695.

Organism-specific databases

GenoListiMYPU_6820.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiAFRCFCT.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Regulation of a restriction and modification system via DNA inversion in Mycoplasma pulmonis."
    Dybvig K., Yu H.
    Mol. Microbiol. 12:547-560(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: KD735-16.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: UAB CTIP.

Entry informationi

Entry nameiSYE_MYCPU
AccessioniPrimary (citable) accession number: P53662
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 27, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.