P53662 (SYE_MYCPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	MYPU_6820

Organism

Mycoplasma pulmonis (strain UAB CTIP) [Complete proteome] [HAMAP]

Taxonomic identifier

272635 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000119609
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022_B
	Motif	252 – 256	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	255	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P53662 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 73026ACA2ED2941B	FASTA	468	55,066
	10 20 30 40 50 60 MKKLRTRYAP SPTGYLHIGG ARTALFNYLL AKHYNGDFII RIEDTDVKRN IADGEASQIE 70 80 90 100 110 120 NLKWLNIEAN ESPLKPNEKY GPYRQSQKLE KYLKIAHELI EKGYAYKAYD NSEELEEQKK 130 140 150 160 170 180 HSEKLGVASF RYQRDFLKIS EEEKQKRDAS GAYSIRVICP KNTTYQWDDL VRGNIAVNSN 190 200 210 220 230 240 DIGDWIIIKS DDYPTYNFAV VIDDIDMEIS HILRGEEHIT NTPKQMMIYD YLNAPKPLFG 250 260 270 280 290 300 HLTIITNMEG KKLSKRDLSL KQFIHEYKEE GYNSQAIFNF LTLLGWTDEK ARELMDHDEI 310 320 330 340 350 360 IKSFLYTRLS KSPSKFDITK MQWFSKQYWK NTPNEELIKI LNLNDYDNDW INLFLDLYKE 370 380 390 400 410 420 NIYSLNQLKN YLKIYKQANL NQEKDLDLND AEKNVVKSFS SYIDYSNFSV NQIQEAINKT 430 440 450 460 QEKLSIKGKN LFLPIRKATT FQEHGPELAK AIYLFGSEII EKRMKKWK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regulation of a restriction and modification system via DNA inversion in Mycoplasma pulmonis." Dybvig K., Yu H. Mol. Microbiol. 12:547-560(1994) [PubMed: 7934878] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: KD735-16.
[2]	"The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis." Chambaud I., Heilig R., Ferris S., Barbe V., Samson D., Galisson F., Moszer I., Dybvig K., Wroblewski H., Viari A., Rocha E.P.C., Blanchard A. Nucleic Acids Res. 29:2145-2153(2001) [PubMed: 11353084] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UAB CTIP.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L25415 Genomic DNA. Translation: AAA65629.1. AL445565 Genomic DNA. Translation: CAC13855.1.
PIR	S49391.
RefSeq	NP_326513.1. NC_002771.1.
3D structure databases
ProteinModelPortal	P53662.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	911061.
GenomeReviews	Gene locus MYPU_6820 in contig AL445566_GR.
KEGG	mpu:MYPU_6820.
PATRIC	20024318. VBIMycPul29704_0695.
Organism-specific databases
GenoList	MYPU_6820.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	ESIIQFV.
PhylomeDB	P53662.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	MPUL272635:MYPU_6820-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_MYCPU

Accession

Primary (citable) accession number: P53662

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program