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P53657

- KPYR_MOUSE

UniProt

P53657 - KPYR_MOUSE

Protein

Pyruvate kinase PKLR

Gene

Pklr

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Plays a key role in glycolysis.By similarity

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.
    Potassium.

    Enzyme regulationi

    Allosterically activated by fructose 1,6-bisphosphate.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei116 – 1161SubstrateBy similarity
    Metal bindingi118 – 1181PotassiumBy similarity
    Metal bindingi120 – 1201PotassiumBy similarity
    Metal bindingi156 – 1561PotassiumBy similarity
    Metal bindingi157 – 1571Potassium; via carbonyl oxygenBy similarity
    Sitei313 – 3131Transition state stabilizerBy similarity
    Metal bindingi315 – 3151MagnesiumBy similarity
    Binding sitei338 – 3381Substrate; via amide nitrogenBy similarity
    Metal bindingi339 – 3391MagnesiumBy similarity
    Binding sitei339 – 3391Substrate; via amide nitrogenBy similarity
    Binding sitei371 – 3711SubstrateBy similarity
    Binding sitei525 – 5251Allosteric activatorBy similarity
    Binding sitei532 – 5321Allosteric activatorBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: MGI

    GO - Biological processi

    1. endocrine pancreas development Source: Reactome
    2. glycolytic process Source: UniProtKB-UniPathway
    3. response to other organism Source: MGI

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    ReactomeiREACT_214529. Regulation of gene expression in beta cells.
    SABIO-RKP53657.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase PKLR (EC:2.7.1.40)
    Alternative name(s):
    L-PK
    Pyruvate kinase isozymes L/R
    Gene namesi
    Name:Pklr
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:97604. Pklr.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 574574Pyruvate kinase PKLRPRO_0000112095Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei43 – 431Phosphoserine; by PKABy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53657.
    PaxDbiP53657.
    PRIDEiP53657.

    PTM databases

    PhosphoSiteiP53657.

    Expressioni

    Gene expression databases

    CleanExiMM_PKLR.
    GenevestigatoriP53657.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    IntActiP53657. 5 interactions.
    MINTiMINT-1854544.

    Structurei

    3D structure databases

    ProteinModelPortaliP53657.
    SMRiP53657. Positions 57-573.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni475 – 4806Allosteric activator bindingBy similarity
    Regioni559 – 5646Allosteric activator bindingBy similarity

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0469.
    HOGENOMiHOG000021559.
    HOVERGENiHBG000941.
    InParanoidiP53657.
    PhylomeDBiP53657.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform R-type (identifier: P53657-1) [UniParc]FASTAAdd to Basket

    Also known as: PKR

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSVQENELPQ QLWPWIFKSQ KDLAKSALSG APGGPAGYLR RASVAQLTQE    50
    LGTAFFQQQQ LPAAMADTFL EHLCLLDIDS EPVAARSTSI IATIGPASRS 100
    VDRLKEMIKA GMNIARLNFS HGSHEYHAES IANIREAAES FATSPLSYRP 150
    VAIALDTKGP EIRTGVLQGG PESEVEIVKG SQVLVTVDPK FRTRGDAKTV 200
    WVDYHNITQV VAVGGRIYID DGLISLVVRK IGPEGLVTEV EHGGFLGNRK 250
    GVNLPNAEVD LPGLSEQDLL DLRFGVEHYV DIIFASFVRK ASDVVAVRDA 300
    LGPEGRGIKI ISKIENHEGV KKFDEILEVS DGIMMARGDL GIEIPAEKVF 350
    LAQKMMIGRC NLAGKPVVCA TQMLESMITK ARPTRAETSD VANAVLDGAD 400
    CIMLSGETAK GSFPVEAVKM QHAIAREAEA AVYHRQLFEE LRRAAPLSRD 450
    PTEVTAIGAV EASFKCCAAA IIVLTKTGRS AQLLSRYRPR AAVIAVTRSA 500
    QAARQVHLSR GVFPLLYREP PEAVWADDVD RRVQFGIESG KLRGFLRVGD 550
    LVIVVTGWRP GSGYTNIMRV LTIS 574
    Length:574
    Mass (Da):62,309
    Last modified:October 1, 1996 - v1
    Checksum:i6C4689DE954E6166
    GO
    Isoform L-type (identifier: P53657-2)

    Also known as: PKL

    Sequence is not available
    Length:
    Mass (Da):

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S79731 mRNA. Translation: AAB35435.1.
    D63764 mRNA. Translation: BAA23642.1.
    CCDSiCCDS17489.1. [P53657-1]
    UniGeneiMm.383180.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S79731 mRNA. Translation: AAB35435.1 .
    D63764 mRNA. Translation: BAA23642.1 .
    CCDSi CCDS17489.1. [P53657-1 ]
    UniGenei Mm.383180.

    3D structure databases

    ProteinModelPortali P53657.
    SMRi P53657. Positions 57-573.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P53657. 5 interactions.
    MINTi MINT-1854544.

    PTM databases

    PhosphoSitei P53657.

    Proteomic databases

    MaxQBi P53657.
    PaxDbi P53657.
    PRIDEi P53657.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:97604. Pklr.

    Phylogenomic databases

    eggNOGi COG0469.
    HOGENOMi HOG000021559.
    HOVERGENi HBG000941.
    InParanoidi P53657.
    PhylomeDBi P53657.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    Reactomei REACT_214529. Regulation of gene expression in beta cells.
    SABIO-RK P53657.

    Miscellaneous databases

    ChiTaRSi PKLR. mouse.
    PROi P53657.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_PKLR.
    Genevestigatori P53657.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of murine red blood cell-type pyruvate kinase (PK) and molecular characterization of PK deficiency identified in the CBA strain."
      Kanno H., Morimoto M., Fujii H., Tsujimura T., Asai H., Noguchi T., Kitamura Y., Miwa S.
      Blood 86:3205-3210(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: CBA/N.
      Tissue: Bone marrow.

    Entry informationi

    Entry nameiKPYR_MOUSE
    AccessioniPrimary (citable) accession number: P53657
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues.

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3