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P53657

- KPYR_MOUSE

UniProt

P53657 - KPYR_MOUSE

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Protein
Pyruvate kinase PKLR
Gene
Pklr
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Plays a key role in glycolysis By similarity.

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium.
Potassium.

Enzyme regulationi

Allosterically activated by fructose 1,6-bisphosphate By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei116 – 1161Substrate By similarity
Metal bindingi118 – 1181Potassium By similarity
Metal bindingi120 – 1201Potassium By similarity
Metal bindingi156 – 1561Potassium By similarity
Metal bindingi157 – 1571Potassium; via carbonyl oxygen By similarity
Sitei313 – 3131Transition state stabilizer By similarity
Metal bindingi315 – 3151Magnesium By similarity
Binding sitei338 – 3381Substrate; via amide nitrogen By similarity
Metal bindingi339 – 3391Magnesium By similarity
Binding sitei339 – 3391Substrate; via amide nitrogen By similarity
Binding sitei371 – 3711Substrate By similarity
Binding sitei525 – 5251Allosteric activator By similarity
Binding sitei532 – 5321Allosteric activator By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: MGI

GO - Biological processi

  1. endocrine pancreas development Source: Reactome
  2. glycolytic process Source: UniProtKB-UniPathway
  3. response to other organism Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

ReactomeiREACT_214529. Regulation of gene expression in beta cells.
SABIO-RKP53657.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase PKLR (EC:2.7.1.40)
Alternative name(s):
L-PK
Pyruvate kinase isozymes L/R
Gene namesi
Name:Pklr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:97604. Pklr.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 574574Pyruvate kinase PKLR
PRO_0000112095Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei43 – 431Phosphoserine; by PKA By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53657.
PaxDbiP53657.
PRIDEiP53657.

PTM databases

PhosphoSiteiP53657.

Expressioni

Gene expression databases

CleanExiMM_PKLR.
GenevestigatoriP53657.

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

IntActiP53657. 5 interactions.
MINTiMINT-1854544.

Structurei

3D structure databases

ProteinModelPortaliP53657.
SMRiP53657. Positions 57-573.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni475 – 4806Allosteric activator binding By similarity
Regioni559 – 5646Allosteric activator binding By similarity

Sequence similaritiesi

Belongs to the pyruvate kinase family.

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021559.
HOVERGENiHBG000941.
InParanoidiP53657.
PhylomeDBiP53657.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform R-type (identifier: P53657-1) [UniParc]FASTAAdd to Basket

Also known as: PKR

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSVQENELPQ QLWPWIFKSQ KDLAKSALSG APGGPAGYLR RASVAQLTQE    50
LGTAFFQQQQ LPAAMADTFL EHLCLLDIDS EPVAARSTSI IATIGPASRS 100
VDRLKEMIKA GMNIARLNFS HGSHEYHAES IANIREAAES FATSPLSYRP 150
VAIALDTKGP EIRTGVLQGG PESEVEIVKG SQVLVTVDPK FRTRGDAKTV 200
WVDYHNITQV VAVGGRIYID DGLISLVVRK IGPEGLVTEV EHGGFLGNRK 250
GVNLPNAEVD LPGLSEQDLL DLRFGVEHYV DIIFASFVRK ASDVVAVRDA 300
LGPEGRGIKI ISKIENHEGV KKFDEILEVS DGIMMARGDL GIEIPAEKVF 350
LAQKMMIGRC NLAGKPVVCA TQMLESMITK ARPTRAETSD VANAVLDGAD 400
CIMLSGETAK GSFPVEAVKM QHAIAREAEA AVYHRQLFEE LRRAAPLSRD 450
PTEVTAIGAV EASFKCCAAA IIVLTKTGRS AQLLSRYRPR AAVIAVTRSA 500
QAARQVHLSR GVFPLLYREP PEAVWADDVD RRVQFGIESG KLRGFLRVGD 550
LVIVVTGWRP GSGYTNIMRV LTIS 574
Length:574
Mass (Da):62,309
Last modified:October 1, 1996 - v1
Checksum:i6C4689DE954E6166
GO
Isoform L-type (identifier: P53657-2)

Also known as: PKL

Sequence is not available
Length:
Mass (Da):

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S79731 mRNA. Translation: AAB35435.1.
D63764 mRNA. Translation: BAA23642.1.
CCDSiCCDS17489.1. [P53657-1]
UniGeneiMm.383180.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S79731 mRNA. Translation: AAB35435.1 .
D63764 mRNA. Translation: BAA23642.1 .
CCDSi CCDS17489.1. [P53657-1 ]
UniGenei Mm.383180.

3D structure databases

ProteinModelPortali P53657.
SMRi P53657. Positions 57-573.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P53657. 5 interactions.
MINTi MINT-1854544.

PTM databases

PhosphoSitei P53657.

Proteomic databases

MaxQBi P53657.
PaxDbi P53657.
PRIDEi P53657.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

MGIi MGI:97604. Pklr.

Phylogenomic databases

eggNOGi COG0469.
HOGENOMi HOG000021559.
HOVERGENi HBG000941.
InParanoidi P53657.
PhylomeDBi P53657.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
Reactomei REACT_214529. Regulation of gene expression in beta cells.
SABIO-RK P53657.

Miscellaneous databases

ChiTaRSi PKLR. mouse.
PROi P53657.
SOURCEi Search...

Gene expression databases

CleanExi MM_PKLR.
Genevestigatori P53657.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Primary structure of murine red blood cell-type pyruvate kinase (PK) and molecular characterization of PK deficiency identified in the CBA strain."
    Kanno H., Morimoto M., Fujii H., Tsujimura T., Asai H., Noguchi T., Kitamura Y., Miwa S.
    Blood 86:3205-3210(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: CBA/N.
    Tissue: Bone marrow.

Entry informationi

Entry nameiKPYR_MOUSE
AccessioniPrimary (citable) accession number: P53657
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues.

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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