P53657 (KPYR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate kinase isozymes R/L
EC=2.7.1.40

Alternative name(s):
L-PK

Gene names

Name:

Pklr

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	574 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Plays a key role in glycolysis By similarity.
Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium. Potassium.
Enzyme regulation	Allosterically activated by fructose 1,6-bisphosphate By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Miscellaneous	There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium Pyruvate
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endocrine pancreas development Traceable author statement. Source: Reactome glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway response to other organism Inferred from mutant phenotype PubMed 14595440. Source: MGI
Cellular_component	cytosol Traceable author statement. Source: Reactome
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from direct assay PubMed 15155459PubMed 7295297. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Select]

Isoform R-type (identifier: P53657-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform L-type (identifier: P53657-2)

The sequence of this isoform is not available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 574	574	Pyruvate kinase isozymes R/L		PRO_0000112095
Regions
	Region	475 – 480	6	Allosteric activator binding By similarity
	Region	559 – 564	6	Allosteric activator binding By similarity
Sites
	Metal binding	118	1	Potassium By similarity
	Metal binding	120	1	Potassium By similarity
	Metal binding	156	1	Potassium By similarity
	Metal binding	157	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	315	1	Magnesium By similarity
	Metal binding	339	1	Magnesium By similarity
	Binding site	116	1	Substrate By similarity
	Binding site	338	1	Substrate; via amide nitrogen By similarity
	Binding site	339	1	Substrate; via amide nitrogen By similarity
	Binding site	371	1	Substrate By similarity
	Binding site	525	1	Allosteric activator By similarity
	Binding site	532	1	Allosteric activator By similarity
	Site	313	1	Transition state stabilizer By similarity
Amino acid modifications
	Modified residue	43	1	Phosphoserine; by PKA By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform R-type [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 6C4689DE954E6166	FASTA	574	62,309
	10 20 30 40 50 60 MSVQENELPQ QLWPWIFKSQ KDLAKSALSG APGGPAGYLR RASVAQLTQE LGTAFFQQQQ 70 80 90 100 110 120 LPAAMADTFL EHLCLLDIDS EPVAARSTSI IATIGPASRS VDRLKEMIKA GMNIARLNFS 130 140 150 160 170 180 HGSHEYHAES IANIREAAES FATSPLSYRP VAIALDTKGP EIRTGVLQGG PESEVEIVKG 190 200 210 220 230 240 SQVLVTVDPK FRTRGDAKTV WVDYHNITQV VAVGGRIYID DGLISLVVRK IGPEGLVTEV 250 260 270 280 290 300 EHGGFLGNRK GVNLPNAEVD LPGLSEQDLL DLRFGVEHYV DIIFASFVRK ASDVVAVRDA 310 320 330 340 350 360 LGPEGRGIKI ISKIENHEGV KKFDEILEVS DGIMMARGDL GIEIPAEKVF LAQKMMIGRC 370 380 390 400 410 420 NLAGKPVVCA TQMLESMITK ARPTRAETSD VANAVLDGAD CIMLSGETAK GSFPVEAVKM 430 440 450 460 470 480 QHAIAREAEA AVYHRQLFEE LRRAAPLSRD PTEVTAIGAV EASFKCCAAA IIVLTKTGRS 490 500 510 520 530 540 AQLLSRYRPR AAVIAVTRSA QAARQVHLSR GVFPLLYREP PEAVWADDVD RRVQFGIESG 550 560 570 KLRGFLRVGD LVIVVTGWRP GSGYTNIMRV LTIS
	Isoform L-type (Sequence not available).		–	–

References

[1]	"Primary structure of murine red blood cell-type pyruvate kinase (PK) and molecular characterization of PK deficiency identified in the CBA strain." Kanno H., Morimoto M., Fujii H., Tsujimura T., Asai H., Noguchi T., Kitamura Y., Miwa S. Blood 86:3205-3210(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CBA/N. Tissue: Bone marrow.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S79731 mRNA. Translation: AAB35435.1. D63764 mRNA. Translation: BAA23642.1.
IPI	IPI00133605.
UniGene	Mm.383180.
3D structure databases
ProteinModelPortal	P53657.
SMR	P53657. Positions 57-573.
ModBase	Search...
Protein-protein interaction databases
IntAct	P53657. 1 interaction.
PTM databases
PhosphoSite	P53657.
Proteomic databases
PaxDb	P53657.
PRIDE	P53657.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
MGI	MGI:97604. Pklr.
Phylogenomic databases
eggNOG	COG0469.
HOGENOM	HOG000021559.
HOVERGEN	HBG000941.
InParanoid	P53657.
OrthoDB	EOG40GCQJ.
Enzyme and pathway databases
Reactome	REACT_112621. Metabolism. REACT_127416. Developmental Biology. REACT_13641. Regulation of Beta-Cell Development.
SABIO-RK	P53657.
UniPathway	UPA00109; UER00188.
Gene expression databases
CleanEx	MM_PKLR.
Genevestigator	P53657.
GermOnline	ENSMUSG00000041237. Mus musculus.
Family and domain databases
Gene3D	2.40.33.10. 1 hit. 3.20.20.60. 2 hits. 3.40.1380.20. 1 hit.
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase. IPR011037. Pyrv_Knase-like_insert_dom. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. IPR015795. Pyrv_Knase_C. IPR015806. Pyrv_Knase_insert_dom. [Graphical view]
PANTHER	PTHR11817. PTHR11817. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
SUPFAM	SSF50800. PK_B_barrel_like. 1 hit. SSF52935. Pyruvate_kinase. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PKLR. mouse.
SOURCE	Search...

Entry information

Entry name

KPYR_MOUSE

Accession

Primary (citable) accession number: P53657

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program