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P53657 (KPYR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase PKLR

EC=2.7.1.40
Alternative name(s):
L-PK
Pyruvate kinase isozymes L/R
Gene names
Name:Pklr
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length574 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Plays a key role in glycolysis By similarity.

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium.

Potassium.

Enzyme regulation

Allosterically activated by fructose 1,6-bisphosphate By similarity.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Miscellaneous

There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues.

Sequence similarities

Belongs to the pyruvate kinase family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Select]
Isoform R-type (identifier: P53657-1)

Also known as: PKR;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform L-type (identifier: P53657-2)

Also known as: PKL;

The sequence of this isoform is not available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 574574Pyruvate kinase PKLR
PRO_0000112095

Regions

Region475 – 4806Allosteric activator binding By similarity
Region559 – 5646Allosteric activator binding By similarity

Sites

Metal binding1181Potassium By similarity
Metal binding1201Potassium By similarity
Metal binding1561Potassium By similarity
Metal binding1571Potassium; via carbonyl oxygen By similarity
Metal binding3151Magnesium By similarity
Metal binding3391Magnesium By similarity
Binding site1161Substrate By similarity
Binding site3381Substrate; via amide nitrogen By similarity
Binding site3391Substrate; via amide nitrogen By similarity
Binding site3711Substrate By similarity
Binding site5251Allosteric activator By similarity
Binding site5321Allosteric activator By similarity
Site3131Transition state stabilizer By similarity

Amino acid modifications

Modified residue431Phosphoserine; by PKA By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform R-type (PKR) [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 6C4689DE954E6166

FASTA57462,309
        10         20         30         40         50         60 
MSVQENELPQ QLWPWIFKSQ KDLAKSALSG APGGPAGYLR RASVAQLTQE LGTAFFQQQQ 

        70         80         90        100        110        120 
LPAAMADTFL EHLCLLDIDS EPVAARSTSI IATIGPASRS VDRLKEMIKA GMNIARLNFS 

       130        140        150        160        170        180 
HGSHEYHAES IANIREAAES FATSPLSYRP VAIALDTKGP EIRTGVLQGG PESEVEIVKG 

       190        200        210        220        230        240 
SQVLVTVDPK FRTRGDAKTV WVDYHNITQV VAVGGRIYID DGLISLVVRK IGPEGLVTEV 

       250        260        270        280        290        300 
EHGGFLGNRK GVNLPNAEVD LPGLSEQDLL DLRFGVEHYV DIIFASFVRK ASDVVAVRDA 

       310        320        330        340        350        360 
LGPEGRGIKI ISKIENHEGV KKFDEILEVS DGIMMARGDL GIEIPAEKVF LAQKMMIGRC 

       370        380        390        400        410        420 
NLAGKPVVCA TQMLESMITK ARPTRAETSD VANAVLDGAD CIMLSGETAK GSFPVEAVKM 

       430        440        450        460        470        480 
QHAIAREAEA AVYHRQLFEE LRRAAPLSRD PTEVTAIGAV EASFKCCAAA IIVLTKTGRS 

       490        500        510        520        530        540 
AQLLSRYRPR AAVIAVTRSA QAARQVHLSR GVFPLLYREP PEAVWADDVD RRVQFGIESG 

       550        560        570 
KLRGFLRVGD LVIVVTGWRP GSGYTNIMRV LTIS 

« Hide

Isoform L-type (PKL) (Sequence not available).

References

[1]"Primary structure of murine red blood cell-type pyruvate kinase (PK) and molecular characterization of PK deficiency identified in the CBA strain."
Kanno H., Morimoto M., Fujii H., Tsujimura T., Asai H., Noguchi T., Kitamura Y., Miwa S.
Blood 86:3205-3210(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: CBA/N.
Tissue: Bone marrow.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S79731 mRNA. Translation: AAB35435.1.
D63764 mRNA. Translation: BAA23642.1.
UniGeneMm.383180.

3D structure databases

ProteinModelPortalP53657.
SMRP53657. Positions 57-573.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP53657. 5 interactions.
MINTMINT-1854544.

PTM databases

PhosphoSiteP53657.

Proteomic databases

PaxDbP53657.
PRIDEP53657.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:97604. Pklr.

Phylogenomic databases

eggNOGCOG0469.
HOGENOMHOG000021559.
HOVERGENHBG000941.
InParanoidP53657.
PhylomeDBP53657.

Enzyme and pathway databases

ReactomeREACT_13641. Regulation of Beta-Cell Development.
REACT_188576. Developmental Biology.
REACT_188937. Metabolism.
SABIO-RKP53657.
UniPathwayUPA00109; UER00188.

Gene expression databases

CleanExMM_PKLR.
GenevestigatorP53657.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPKLR. mouse.
PROP53657.
SOURCESearch...

Entry information

Entry nameKPYR_MOUSE
AccessionPrimary (citable) accession number: P53657
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot