Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sterol O-acyltransferase 2

Gene

ARE2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ensures probably most of the acyltransferase activity. Suppression of ARE2 reduces sterol ester levels to 25% of the normal value.

Miscellaneous

Present with 279 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Acyl-CoA + cholesterol = CoA + cholesterol ester.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei579Sequence analysis1

GO - Molecular functioni

  • ergosterol O-acyltransferase activity Source: SGD

GO - Biological processi

  • ergosterol metabolic process Source: SGD

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:YNR019W-MONOMER
YEAST:YNR019W-MONOMER
BRENDAi2.3.1.26 984
ReactomeiR-SCE-1482883 Acyl chain remodeling of DAG and TAG
R-SCE-6798695 Neutrophil degranulation
R-SCE-75109 Triglyceride biosynthesis
R-SCE-8964038 LDL clearance

Chemistry databases

SwissLipidsiSLP:000000707
SLP:000000708

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol O-acyltransferase 2 (EC:2.3.1.26)
Alternative name(s):
Sterol-ester synthase 2
Gene namesi
Name:ARE2
Synonyms:SAT1
Ordered Locus Names:YNR019W
ORF Names:N3206
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNR019W
SGDiS000005302 ARE2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei215 – 235HelicalSequence analysisAdd BLAST21
Transmembranei292 – 312HelicalSequence analysisAdd BLAST21
Transmembranei404 – 424HelicalSequence analysisAdd BLAST21
Transmembranei442 – 462HelicalSequence analysisAdd BLAST21
Transmembranei485 – 505HelicalSequence analysisAdd BLAST21
Transmembranei567 – 587HelicalSequence analysisAdd BLAST21
Transmembranei622 – 642HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002076511 – 642Sterol O-acyltransferase 2Add BLAST642

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei175PhosphoserineCombined sources1
Modified residuei176PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53629
PaxDbiP53629
PRIDEiP53629

PTM databases

iPTMnetiP53629

Interactioni

Protein-protein interaction databases

BioGridi35844, 143 interactors
DIPiDIP-4050N
IntActiP53629, 8 interactors
MINTiP53629
STRINGi4932.YNR019W

Structurei

3D structure databases

ProteinModelPortaliP53629
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000063122
HOGENOMiHOG000210250
InParanoidiP53629
KOiK00637
OMAiQIEYPRT
OrthoDBiEOG092C2MBI

Family and domain databases

InterProiView protein in InterPro
IPR004299 MBOAT_fam
IPR014371 Oat_ACAT_DAG_ARE
PANTHERiPTHR10408 PTHR10408, 1 hit
PfamiView protein in Pfam
PF03062 MBOAT, 1 hit
PIRSFiPIRSF000439 Oat_ACAT_DAG_ARE, 1 hit

Sequencei

Sequence statusi: Complete.

P53629-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKKKDLLEN EQFLRIQKLN AADAGKRQSI TVDDEGELYG LDTSGNSPAN
60 70 80 90 100
EHTATTITQN HSVVASNGDV AFIPGTATEG NTEIVTEEVI ETDDNMFKTH
110 120 130 140 150
VKTLSSKEKA RYRQGSSNFI SYFDDMSFEH RPSILDGSVN EPFKTKFVGP
160 170 180 190 200
TLEKEIRRRE KELMAMRKNL HHRKSSPDAV DSVGKNDGAA PTTVPTAATS
210 220 230 240 250
ETVVTVETTI ISSNFSGLYV AFWMAIAFGA VKALIDYYYQ HNGSFKDSEI
260 270 280 290 300
LKFMTTNLFT VASVDLLMYL STYFVVGIQY LCKWGVLKWG TTGWIFTSIY
310 320 330 340 350
EFLFVIFYMY LTENILKLHW LSKIFLFLHS LVLLMKMHSF AFYNGYLWGI
360 370 380 390 400
KEELQFSKSA LAKYKDSIND PKVIGALEKS CEFCSFELSS QSLSDQTQKF
410 420 430 440 450
PNNISAKSFF WFTMFPTLIY QIEYPRTKEI RWSYVLEKIC AIFGTIFLMM
460 470 480 490 500
IDAQILMYPV AMRALAVRNS EWTGILDRLL KWVGLLVDIV PGFIVMYILD
510 520 530 540 550
FYLIWDAILN CVAELTRFGD RYFYGDWWNC VSWADFSRIW NIPVHKFLLR
560 570 580 590 600
HVYHSSMSSF KLNKSQATLM TFFLSSVVHE LAMYVIFKKL RFYLFFFQML
610 620 630 640
QMPLVALTNT KFMRNRTIIG NVIFWLGICM GPSVMCTLYL TF
Length:642
Mass (Da):74,023
Last modified:October 1, 1996 - v1
Checksum:i035FC4ED9C7CD830
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti80G → D in AAC49441 (PubMed:8798656).Curated1
Sequence conflicti184G → E in AAC49441 (PubMed:8798656).Curated1
Sequence conflicti211I → L in AAC49441 (PubMed:8798656).Curated1
Sequence conflicti612F → S in AAC49441 (PubMed:8798656).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51790 Genomic DNA Translation: AAB02203.1
U55383 Genomic DNA Translation: AAC49441.1
Z71634 Genomic DNA Translation: CAA96298.1
BK006947 Genomic DNA Translation: DAA10560.1
PIRiS63350
RefSeqiNP_014416.1, NM_001183196.1

Genome annotation databases

EnsemblFungiiYNR019W; YNR019W; YNR019W
GeneIDi855753
KEGGisce:YNR019W

Similar proteinsi

Entry informationi

Entry nameiARE2_YEAST
AccessioniPrimary (citable) accession number: P53629
Secondary accession number(s): D6W1J4, Q12673
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health