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Protein

Sterol O-acyltransferase 2

Gene

ARE2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Ensures probably most of the acyltransferase activity. Suppression of ARE2 reduces sterol ester levels to 25% of the normal value.

Catalytic activityi

Acyl-CoA + cholesterol = CoA + cholesterol ester.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei579 – 5791Sequence analysis

GO - Molecular functioni

  • ergosterol O-acyltransferase activity Source: SGD

GO - Biological processi

  • ergosterol metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:YNR019W-MONOMER.
YEAST:YNR019W-MONOMER.
BRENDAi2.3.1.26. 984.
ReactomeiR-SCE-1482883. Acyl chain remodeling of DAG and TAG.
R-SCE-75109. Triglyceride Biosynthesis.

Chemistry

SwissLipidsiSLP:000000707.
SLP:000000708.

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol O-acyltransferase 2 (EC:2.3.1.26)
Alternative name(s):
Sterol-ester synthase 2
Gene namesi
Name:ARE2
Synonyms:SAT1
Ordered Locus Names:YNR019W
ORF Names:N3206
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNR019W.
SGDiS000005302. ARE2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei215 – 23521HelicalSequence analysisAdd
BLAST
Transmembranei292 – 31221HelicalSequence analysisAdd
BLAST
Transmembranei404 – 42421HelicalSequence analysisAdd
BLAST
Transmembranei442 – 46221HelicalSequence analysisAdd
BLAST
Transmembranei485 – 50521HelicalSequence analysisAdd
BLAST
Transmembranei567 – 58721HelicalSequence analysisAdd
BLAST
Transmembranei622 – 64221HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 642642Sterol O-acyltransferase 2PRO_0000207651Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei175 – 1751PhosphoserineCombined sources
Modified residuei176 – 1761PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53629.

PTM databases

iPTMnetiP53629.

Interactioni

Protein-protein interaction databases

BioGridi35844. 92 interactions.
DIPiDIP-4050N.
IntActiP53629. 5 interactions.
MINTiMINT-476870.

Structurei

3D structure databases

ProteinModelPortaliP53629.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000063122.
HOGENOMiHOG000210250.
InParanoidiP53629.
KOiK00637.
OMAiQIEYPRT.
OrthoDBiEOG092C2MBI.

Family and domain databases

InterProiIPR004299. MBOAT_fam.
IPR014371. Oat_ACAT_DAG_ARE.
[Graphical view]
PANTHERiPTHR10408. PTHR10408. 1 hit.
PfamiPF03062. MBOAT. 1 hit.
[Graphical view]
PIRSFiPIRSF000439. Oat_ACAT_DAG_ARE. 1 hit.

Sequencei

Sequence statusi: Complete.

P53629-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKKKDLLEN EQFLRIQKLN AADAGKRQSI TVDDEGELYG LDTSGNSPAN
60 70 80 90 100
EHTATTITQN HSVVASNGDV AFIPGTATEG NTEIVTEEVI ETDDNMFKTH
110 120 130 140 150
VKTLSSKEKA RYRQGSSNFI SYFDDMSFEH RPSILDGSVN EPFKTKFVGP
160 170 180 190 200
TLEKEIRRRE KELMAMRKNL HHRKSSPDAV DSVGKNDGAA PTTVPTAATS
210 220 230 240 250
ETVVTVETTI ISSNFSGLYV AFWMAIAFGA VKALIDYYYQ HNGSFKDSEI
260 270 280 290 300
LKFMTTNLFT VASVDLLMYL STYFVVGIQY LCKWGVLKWG TTGWIFTSIY
310 320 330 340 350
EFLFVIFYMY LTENILKLHW LSKIFLFLHS LVLLMKMHSF AFYNGYLWGI
360 370 380 390 400
KEELQFSKSA LAKYKDSIND PKVIGALEKS CEFCSFELSS QSLSDQTQKF
410 420 430 440 450
PNNISAKSFF WFTMFPTLIY QIEYPRTKEI RWSYVLEKIC AIFGTIFLMM
460 470 480 490 500
IDAQILMYPV AMRALAVRNS EWTGILDRLL KWVGLLVDIV PGFIVMYILD
510 520 530 540 550
FYLIWDAILN CVAELTRFGD RYFYGDWWNC VSWADFSRIW NIPVHKFLLR
560 570 580 590 600
HVYHSSMSSF KLNKSQATLM TFFLSSVVHE LAMYVIFKKL RFYLFFFQML
610 620 630 640
QMPLVALTNT KFMRNRTIIG NVIFWLGICM GPSVMCTLYL TF
Length:642
Mass (Da):74,023
Last modified:October 1, 1996 - v1
Checksum:i035FC4ED9C7CD830
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti80 – 801G → D in AAC49441 (PubMed:8798656).Curated
Sequence conflicti184 – 1841G → E in AAC49441 (PubMed:8798656).Curated
Sequence conflicti211 – 2111I → L in AAC49441 (PubMed:8798656).Curated
Sequence conflicti612 – 6121F → S in AAC49441 (PubMed:8798656).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51790 Genomic DNA. Translation: AAB02203.1.
U55383 Genomic DNA. Translation: AAC49441.1.
Z71634 Genomic DNA. Translation: CAA96298.1.
BK006947 Genomic DNA. Translation: DAA10560.1.
PIRiS63350.
RefSeqiNP_014416.1. NM_001183196.1.

Genome annotation databases

EnsemblFungiiYNR019W; YNR019W; YNR019W.
GeneIDi855753.
KEGGisce:YNR019W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51790 Genomic DNA. Translation: AAB02203.1.
U55383 Genomic DNA. Translation: AAC49441.1.
Z71634 Genomic DNA. Translation: CAA96298.1.
BK006947 Genomic DNA. Translation: DAA10560.1.
PIRiS63350.
RefSeqiNP_014416.1. NM_001183196.1.

3D structure databases

ProteinModelPortaliP53629.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35844. 92 interactions.
DIPiDIP-4050N.
IntActiP53629. 5 interactions.
MINTiMINT-476870.

Chemistry

SwissLipidsiSLP:000000707.
SLP:000000708.

PTM databases

iPTMnetiP53629.

Proteomic databases

MaxQBiP53629.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNR019W; YNR019W; YNR019W.
GeneIDi855753.
KEGGisce:YNR019W.

Organism-specific databases

EuPathDBiFungiDB:YNR019W.
SGDiS000005302. ARE2.

Phylogenomic databases

GeneTreeiENSGT00530000063122.
HOGENOMiHOG000210250.
InParanoidiP53629.
KOiK00637.
OMAiQIEYPRT.
OrthoDBiEOG092C2MBI.

Enzyme and pathway databases

BioCyciMetaCyc:YNR019W-MONOMER.
YEAST:YNR019W-MONOMER.
BRENDAi2.3.1.26. 984.
ReactomeiR-SCE-1482883. Acyl chain remodeling of DAG and TAG.
R-SCE-75109. Triglyceride Biosynthesis.

Miscellaneous databases

PROiP53629.

Family and domain databases

InterProiIPR004299. MBOAT_fam.
IPR014371. Oat_ACAT_DAG_ARE.
[Graphical view]
PANTHERiPTHR10408. PTHR10408. 1 hit.
PfamiPF03062. MBOAT. 1 hit.
[Graphical view]
PIRSFiPIRSF000439. Oat_ACAT_DAG_ARE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiARE2_YEAST
AccessioniPrimary (citable) accession number: P53629
Secondary accession number(s): D6W1J4, Q12673
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 279 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.