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Protein

Intracellular exo-alpha-(1->5)-L-arabinofuranosidase

Gene

abfA

Organism
Streptomyces lividans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the cleavage of terminal alpha-(1->5)-arabinofuranosyl bonds in different hemicellulosic homopolysaccharides (arabino-oligoxylosides, branched and debranched arabinans). It acts rapidly on the short-chain arabino-oligoxylosides from digestion of xylan with xylanases. It hydrolyzes slowly arabinan and arabinoxylan from wheat and rye flour.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.1 Publication

Kineticsi

  1. KM=0.6 mM for p-nitrophenyl alpha-L-arabinofuranoside (pNP-Araf) (at 60 degrees Celsius and at pH 6)1 Publication
  1. Vmax=180 µmol/min/mg enzyme (at 60 degrees Celsius and at pH 6)1 Publication

pH dependencei

Optimum pH is 6.0.1 Publication

Temperature dependencei

Optimum temperature is 60 degrees Celsius.1 Publication

Pathway:iL-arabinan degradation

This protein is involved in the pathway L-arabinan degradation, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway L-arabinan degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei27 – 271SubstrateBy similarity
Binding sitei72 – 721Substrate; via amide nitrogenBy similarity
Active sitei175 – 1751Proton donor/acceptorBy similarity
Binding sitei246 – 2461SubstrateBy similarity
Active sitei294 – 2941NucleophileBy similarity
Binding sitei294 – 2941SubstrateBy similarity
Sitei298 – 2981Important for substrate recognitionBy similarity
Binding sitei352 – 3521SubstrateBy similarity
Sitei352 – 3521Important for substrate recognitionBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BRENDAi3.2.1.55. 6052.
UniPathwayiUPA00667.

Protein family/group databases

CAZyiGH51. Glycoside Hydrolase Family 51.

Names & Taxonomyi

Protein namesi
Recommended name:
Intracellular exo-alpha-(1->5)-L-arabinofuranosidase (EC:3.2.1.55)
Short name:
ABF
Alternative name(s):
Alpha-L-AF
Arabinoxylan arabinofuranohydrolase
Intracellular arabinan exo-alpha-(1->5)-L-arabinosidase
Short name:
Arabinosidase
Gene namesi
Name:abfA
OrganismiStreptomyces lividans
Taxonomic identifieri1916 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 662662Intracellular exo-alpha-(1->5)-L-arabinofuranosidasePRO_0000057704Add
BLAST

Interactioni

Subunit structurei

Homohexamer; trimer of dimers.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP53627.
SMRiP53627. Positions 3-500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni174 – 1752Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 51 family.Curated

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR010720. Alpha-L-AF_C.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF06964. Alpha-L-AF_C. 1 hit.
[Graphical view]
SMARTiSM00813. Alpha-L-AF_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

P53627-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTARFTLDP AFTVGAVNPR LFGSFVEHLG RCVYTGVFEP GHPTADAEGL
60 70 80 90 100
RQDVLELVRE LGVTAVRYPG GNFVSGYKWE DSVGPVEDRP RRLDLAWRST
110 120 130 140 150
ETNRFGLSEY IAFLKKIGPQ AEPMMAVNLG TRGVAEALEL QEYANHPSGT
160 170 180 190 200
ALSDLRAEHG DKDPFGIRLW CLGNEMDGPW QTGHKTAEEY GRVAAETARA
210 220 230 240 250
MRQIDPDVEL VACGSSGQSM ETFAEWEATV LKETYDLVDH ISLHAYYEPH
260 270 280 290 300
DGDVDSFLAS AVDMESFIEN VVATCDHVGA RLKSKKKINL SFDEWNVWYM
310 320 330 340 350
TKTQAEVSAL DWPEAPRLLE DNYSVMDAVV FGSLLIALLR HADRVTVACL
360 370 380 390 400
AQLVNVIAPI MTEPGGPAWR QTTFFPFSQA SKYGRGEVLD VRVDSPTYDT
410 420 430 440 450
AKYGEADLLH ATAVVRARRS VTVFAVNRSR TGALPLEVAL SGLELTEVVE
460 470 480 490 500
HSALADADPD ARNTLAEPER VVPHPVDGTS LRDGRLTAAL EPLSWNSIRC
510 520 530 540 550
ADPAPGQPPR RPGEGTGFTG TPPAAPPSSS SAPRPDPTAR RSPDRTARAR
560 570 580 590 600
VLAAARVRRM PFGRTKVCGA PVRPPTYAPR FQPFRKTWTR WAPAPRSGSP
610 620 630 640 650
SRRSPTEASI PGGTSSRNVV RYQVTPWRRS PPGSAPGTPA PTRRRRTRAG
660
ASRGAPRTAR RC
Length:662
Mass (Da):72,497
Last modified:October 1, 1996 - v1
Checksum:iDAAF66A577C1D6D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04630 Genomic DNA. Translation: AAA61708.1.
PIRiS55274.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04630 Genomic DNA. Translation: AAA61708.1.
PIRiS55274.

3D structure databases

ProteinModelPortaliP53627.
SMRiP53627. Positions 3-500.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH51. Glycoside Hydrolase Family 51.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00667.
BRENDAi3.2.1.55. 6052.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR010720. Alpha-L-AF_C.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF06964. Alpha-L-AF_C. 1 hit.
[Graphical view]
SMARTiSM00813. Alpha-L-AF_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Purification and characterization of an alpha-L-arabinofuranosidase from Streptomyces lividans 66 and DNA sequence of the gene (abfA)."
    Manin C., Shareek F., Morosoli R., Kluepfel D.
    Biochem. J. 302:443-449(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-23, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, SUBSTRATE SPECIFICITY, SUBUNIT.
    Strain: 66 / 1326.

Entry informationi

Entry nameiIABF_STRLI
AccessioniPrimary (citable) accession number: P53627
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 24, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.