Reviewed, UniProtKB/Swiss-Prot P53627 (ABFA_STRLI)

Names and origin

Protein names

Recommended name:
    Alpha-N-arabinofuranosidase
      Short name=Arabinosidase
      Short name=ABF
    EC=3.2.1.55
Alternative name(s):
    Alpha-L-AF

Gene names

Name:

abfA

Organism

Streptomyces lividans

Taxonomic identifier

1916 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	662 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Important role in the degradation of arabinose-containing hemicelluloses. Hydrolyzes rapidly the short-chain arabino-oligoxylosides from digestion of xylan with xylanases. Acts slowly on arabinan and arabinoxylan from wheat and rye flour to release L-arabinofuranose. Does not act on oat-spelts xylan or arabinogalactan.
Catalytic activity	Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.
Subunit structure	Oligomer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glycosyl hydrolase 51 family.
Biophysicochemical properties	pH dependence: Optimum pH is 6.0. Temperature dependence: Optimum temperature is 60 degrees Celsius.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	L-arabinose metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-N-arabinofuranosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 662	662	Alpha-N-arabinofuranosidase		PRO_0000057704

Sequences

	Sequence		Length	Mass (Da)
	P53627-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: DAAF66A577C1D6D1	FASTA	662	72,497
	10 20 30 40 50 60 MRTARFTLDP AFTVGAVNPR LFGSFVEHLG RCVYTGVFEP GHPTADAEGL RQDVLELVRE 70 80 90 100 110 120 LGVTAVRYPG GNFVSGYKWE DSVGPVEDRP RRLDLAWRST ETNRFGLSEY IAFLKKIGPQ 130 140 150 160 170 180 AEPMMAVNLG TRGVAEALEL QEYANHPSGT ALSDLRAEHG DKDPFGIRLW CLGNEMDGPW 190 200 210 220 230 240 QTGHKTAEEY GRVAAETARA MRQIDPDVEL VACGSSGQSM ETFAEWEATV LKETYDLVDH 250 260 270 280 290 300 ISLHAYYEPH DGDVDSFLAS AVDMESFIEN VVATCDHVGA RLKSKKKINL SFDEWNVWYM 310 320 330 340 350 360 TKTQAEVSAL DWPEAPRLLE DNYSVMDAVV FGSLLIALLR HADRVTVACL AQLVNVIAPI 370 380 390 400 410 420 MTEPGGPAWR QTTFFPFSQA SKYGRGEVLD VRVDSPTYDT AKYGEADLLH ATAVVRARRS 430 440 450 460 470 480 VTVFAVNRSR TGALPLEVAL SGLELTEVVE HSALADADPD ARNTLAEPER VVPHPVDGTS 490 500 510 520 530 540 LRDGRLTAAL EPLSWNSIRC ADPAPGQPPR RPGEGTGFTG TPPAAPPSSS SAPRPDPTAR 550 560 570 580 590 600 RSPDRTARAR VLAAARVRRM PFGRTKVCGA PVRPPTYAPR FQPFRKTWTR WAPAPRSGSP 610 620 630 640 650 660 SRRSPTEASI PGGTSSRNVV RYQVTPWRRS PPGSAPGTPA PTRRRRTRAG ASRGAPRTAR RC

References

[1]

"Purification and characterization of an alpha-L-arabinofuranosidase from Streptomyces lividans 66 and DNA sequence of the gene (abfA)." Manin C., Shareek F., Morosoli R., Kluepfel D. Biochem. J. 302:443-449(1994) [PubMed: 8092996] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-23. Strain: 66 / 1326.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U04630 Genomic DNA. Translation: AAA61708.1.
PIR	S55274.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH51. Glycoside Hydrolase Family 51.
Enzyme and pathway databases
BRENDA	3.2.1.55. 15238.
Family and domain databases
InterPro	IPR010720. Alpha-L-AF_C. [Graphical view]
Pfam	PF06964. Alpha-L-AF_C. 1 hit. [Graphical view]
SMART	SM00813. Alpha-L-AF_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ABFA_STRLI

Accession

Primary (citable) accession number: P53627

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)