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P53627

- IABF_STRLI

UniProt

P53627 - IABF_STRLI

Protein

Intracellular exo-alpha-(1->5)-L-arabinofuranosidase

Gene

abfA

Organism
Streptomyces lividans
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the cleavage of terminal alpha-(1->5)-arabinofuranosyl bonds in different hemicellulosic homopolysaccharides (arabino-oligoxylosides, branched and debranched arabinans). It acts rapidly on the short-chain arabino-oligoxylosides from digestion of xylan with xylanases. It hydrolyzes slowly arabinan and arabinoxylan from wheat and rye flour.1 Publication

    Catalytic activityi

    Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.1 Publication

    Kineticsi

    1. KM=0.6 mM for p-nitrophenyl alpha-L-arabinofuranoside (pNP-Araf) (at 60 degrees Celsius and at pH 6)1 Publication

    Vmax=180 µmol/min/mg enzyme (at 60 degrees Celsius and at pH 6)1 Publication

    pH dependencei

    Optimum pH is 6.0.1 Publication

    Temperature dependencei

    Optimum temperature is 60 degrees Celsius.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei27 – 271SubstrateBy similarity
    Binding sitei72 – 721Substrate; via amide nitrogenBy similarity
    Active sitei175 – 1751Proton donor/acceptorBy similarity
    Binding sitei246 – 2461SubstrateBy similarity
    Active sitei294 – 2941NucleophileBy similarity
    Binding sitei294 – 2941SubstrateBy similarity
    Sitei298 – 2981Important for substrate recognitionBy similarity
    Binding sitei352 – 3521SubstrateBy similarity
    Sitei352 – 3521Important for substrate recognitionBy similarity

    GO - Molecular functioni

    1. alpha-L-arabinofuranosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. arabinan catabolic process Source: UniProtKB-UniPathway
    2. L-arabinose metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism

    Enzyme and pathway databases

    UniPathwayiUPA00667.

    Protein family/group databases

    CAZyiGH51. Glycoside Hydrolase Family 51.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Intracellular exo-alpha-(1->5)-L-arabinofuranosidase (EC:3.2.1.55)
    Short name:
    ABF
    Alternative name(s):
    Alpha-L-AF
    Arabinoxylan arabinofuranohydrolase
    Intracellular arabinan exo-alpha-(1->5)-L-arabinosidase
    Short name:
    Arabinosidase
    Gene namesi
    Name:abfA
    OrganismiStreptomyces lividans
    Taxonomic identifieri1916 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 662662Intracellular exo-alpha-(1->5)-L-arabinofuranosidasePRO_0000057704Add
    BLAST

    Interactioni

    Subunit structurei

    Homohexamer; trimer of dimers.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP53627.
    SMRiP53627. Positions 3-500.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni174 – 1752Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 51 family.Curated

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR010720. Alpha-L-AF_C.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF06964. Alpha-L-AF_C. 1 hit.
    [Graphical view]
    SMARTiSM00813. Alpha-L-AF_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P53627-1 [UniParc]FASTAAdd to Basket

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    MRTARFTLDP AFTVGAVNPR LFGSFVEHLG RCVYTGVFEP GHPTADAEGL    50
    RQDVLELVRE LGVTAVRYPG GNFVSGYKWE DSVGPVEDRP RRLDLAWRST 100
    ETNRFGLSEY IAFLKKIGPQ AEPMMAVNLG TRGVAEALEL QEYANHPSGT 150
    ALSDLRAEHG DKDPFGIRLW CLGNEMDGPW QTGHKTAEEY GRVAAETARA 200
    MRQIDPDVEL VACGSSGQSM ETFAEWEATV LKETYDLVDH ISLHAYYEPH 250
    DGDVDSFLAS AVDMESFIEN VVATCDHVGA RLKSKKKINL SFDEWNVWYM 300
    TKTQAEVSAL DWPEAPRLLE DNYSVMDAVV FGSLLIALLR HADRVTVACL 350
    AQLVNVIAPI MTEPGGPAWR QTTFFPFSQA SKYGRGEVLD VRVDSPTYDT 400
    AKYGEADLLH ATAVVRARRS VTVFAVNRSR TGALPLEVAL SGLELTEVVE 450
    HSALADADPD ARNTLAEPER VVPHPVDGTS LRDGRLTAAL EPLSWNSIRC 500
    ADPAPGQPPR RPGEGTGFTG TPPAAPPSSS SAPRPDPTAR RSPDRTARAR 550
    VLAAARVRRM PFGRTKVCGA PVRPPTYAPR FQPFRKTWTR WAPAPRSGSP 600
    SRRSPTEASI PGGTSSRNVV RYQVTPWRRS PPGSAPGTPA PTRRRRTRAG 650
    ASRGAPRTAR RC 662
    Length:662
    Mass (Da):72,497
    Last modified:October 1, 1996 - v1
    Checksum:iDAAF66A577C1D6D1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U04630 Genomic DNA. Translation: AAA61708.1.
    PIRiS55274.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U04630 Genomic DNA. Translation: AAA61708.1 .
    PIRi S55274.

    3D structure databases

    ProteinModelPortali P53627.
    SMRi P53627. Positions 3-500.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH51. Glycoside Hydrolase Family 51.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00667 .

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR010720. Alpha-L-AF_C.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF06964. Alpha-L-AF_C. 1 hit.
    [Graphical view ]
    SMARTi SM00813. Alpha-L-AF_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Purification and characterization of an alpha-L-arabinofuranosidase from Streptomyces lividans 66 and DNA sequence of the gene (abfA)."
      Manin C., Shareek F., Morosoli R., Kluepfel D.
      Biochem. J. 302:443-449(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-23, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, SUBSTRATE SPECIFICITY, SUBUNIT.
      Strain: 66 / 1326.

    Entry informationi

    Entry nameiIABF_STRLI
    AccessioniPrimary (citable) accession number: P53627
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3