Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P53626

- E13B_TRIHA

UniProt

P53626 - E13B_TRIHA

Protein

Glucan endo-1,3-beta-glucosidase BGN13.1

Gene

bgn13.1

Organism
Trichoderma harzianum (Hypocrea lixii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in mycoparasitism, hydrolyzes yeast and fungal cell walls. Classified as a small-oligosaccharide-producing type based its the end products: glucose, laminaribiose or laminaritetraose.

    Catalytic activityi

    Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

    Enzyme regulationi

    Inhibited by glucose.

    Temperature dependencei

    Optimum temperature is 40 degrees Celsius. Inactive at 55 degrees Celsius.

    GO - Molecular functioni

    1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH55. Glycoside Hydrolase Family 55.
    mycoCLAPiLAM55M_TRIHA.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucan endo-1,3-beta-glucosidase BGN13.1 (EC:3.2.1.39)
    Alternative name(s):
    (1->3)-beta-glucan endohydrolase BGN13.1
    Short name:
    (1->3)-beta-glucanase BGN13.1
    Basic beta-1,3-endoglucanase BGN13.1
    Gene namesi
    Name:bgn13.1
    OrganismiTrichoderma harzianum (Hypocrea lixii)
    Taxonomic identifieri5544 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesHypocreaceaeTrichoderma

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Sequence AnalysisAdd
    BLAST
    Propeptidei17 – 3317PRO_0000012227Add
    BLAST
    Chaini34 – 762729Glucan endo-1,3-beta-glucosidase BGN13.1PRO_0000012228Add
    BLAST

    Post-translational modificationi

    Does not seem to be glycosylated.

    Keywords - PTMi

    Cleavage on pair of basic residues

    Structurei

    3D structure databases

    ProteinModelPortaliP53626.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi520 – 762243Cys-richAdd
    BLAST

    Domaini

    The C-terminal cysteine-rich region may function as a fungal cell wall binding domain.

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 55 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.160.20.10. 1 hit.
    InterProiIPR012334. Pectin_lyas_fold.
    IPR011050. Pectin_lyase_fold/virulence.
    [Graphical view]
    SUPFAMiSSF51126. SSF51126. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P53626-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKLTALVAL LLGAASATPT PSPPASDEGI TKRATSFYYP NMDHVNAPRG    50
    FAPDLDGDFN YPIYQTVNAG DGNALQNAIT TDGKGGSRHP QWFASQPRVV 100
    YIPPGTYTIS KTLRFNTDTI LMGDPTNPPI IKAAAGFSGD QTLISAQDPS 150
    TNEKGELSFA VAIKNVVLDT TAIPGGNSFT ALWWGVAQAA HLQNVRITMS 200
    SSSGGNGHTG IRMGRGSTLG LADVRVERGQ NGIWIDGHQQ ASFHNIYFFQ 250
    NTIGMLISSG NTFSIFSSTF DTCGTAFPTL AGSPWIALID AKSINSGVTF 300
    TTNQFPSFMI ENLTKDNGTP VVVVRGSTLV GASSHVNTYS YGNTVGRNPT 350
    YGDVTSSNTR PSALAPGGRY PYVAPPTYGD LPISSFLNVK DPAQNGNRQV 400
    KGDNTINEAD TLNAILELAA SQNKVAYFPF GKYRVDSTLF IPKGSRIVGE 450
    AWATITGNGN FFKNENSPQP VVSVGRAGDV GIAQLQDLRV TTNDVLPGAI 500
    LVQFNMAGNN PGDVALWNSL VTVGGTRGAQ ALANACTNNS NECKGAFIGI 550
    HVAKGSSPYI QNVWELGLRD HIAENFSGGT SHRRERWNFG PIRRNATCLY 600
    PIGSGHWWLY QLNLHNAANV VVSLLQAETN YHQGANTQQI PPAPWVANVG 650
    TWGDPDFSWC NGGDKRCRMG PANFINGGSN IYTYASAAWA FFSGPGQGCA 700
    QFECQQTIHW IASTPSNLQA FGLCSKDSVN TLRLGDGTFI NTQNGYTGGW 750
    TPGGGDVARY TT 762
    Length:762
    Mass (Da):81,247
    Last modified:October 1, 1996 - v1
    Checksum:i0CC25C3C10897AF1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti40 – 401P → F AA sequence (PubMed:7592488)Curated
    Sequence conflicti759 – 7591R → P AA sequence (PubMed:7592488)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X84085 mRNA. Translation: CAA58889.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X84085 mRNA. Translation: CAA58889.1 .

    3D structure databases

    ProteinModelPortali P53626.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH55. Glycoside Hydrolase Family 55.
    mycoCLAPi LAM55M_TRIHA.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.160.20.10. 1 hit.
    InterProi IPR012334. Pectin_lyas_fold.
    IPR011050. Pectin_lyase_fold/virulence.
    [Graphical view ]
    SUPFAMi SSF51126. SSF51126. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "A novel endo-beta-1,3-glucanase, BGN13.1, involved in the mycoparasitism of Trichoderma harzianum."
      de la Cruz J., Pintor-Toro J.A., Benitez T., Llobell A., Romero L.C.
      J. Bacteriol. 177:6937-6945(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Strain: ATCC 48131 / CBS 354.33 / CECT 2413 / VTT D-80150.

    Entry informationi

    Entry nameiE13B_TRIHA
    AccessioniPrimary (citable) accession number: P53626
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3