Reviewed, UniProtKB/Swiss-Prot P53626 (E13B_TRIHA)

Names and origin

Protein names

Recommended name:
    Glucan endo-1,3-beta-glucosidase BGN13.1
    EC=3.2.1.39
Alternative name(s):
    (1->3)-beta-glucan endohydrolase BGN13.1
      Short name=(1->3)-beta-glucanase BGN13.1
    Basic beta-1,3-endoglucanase BGN13.1

Gene names

Name:

bgn13.1

Organism

Trichoderma harzianum (Hypocrea lixii)

Taxonomic identifier

5544 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Hypocreaceae › Hypocrea

Protein attributes

Sequence length	762 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in mycoparasitism, hydrolyzes yeast and fungal cell walls. Classified as a small-oligosaccharide-producing type based its the end products: glucose, laminaribiose or laminaritetraose.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Enzyme regulation	Inhibited by glucose.
Subcellular location	Secreted.
Domain	The C-terminal cysteine-rich region may function as a fungal cell wall binding domain.
Post-translational modification	Does not seem to be glycosylated.
Sequence similarities	Belongs to the glycosyl hydrolase 55 family.
Biophysicochemical properties	Temperature dependence: Optimum temperature is 40 degrees Celsius. Inactive at 55 degrees Celsius.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Cleavage on pair of basic residues
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Propeptide	17 – 33	17			PRO_0000012227
	Chain	34 – 762	729	Glucan endo-1,3-beta-glucosidase BGN13.1		PRO_0000012228
Regions
	Compositional bias	520 – 762	243	Cys-rich
Experimental info
	Sequence conflict	40	1	P → F AA sequence Ref.1
	Sequence conflict	759	1	R → P AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P53626-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 0CC25C3C10897AF1	FASTA	762	81,247
	10 20 30 40 50 60 MLKLTALVAL LLGAASATPT PSPPASDEGI TKRATSFYYP NMDHVNAPRG FAPDLDGDFN 70 80 90 100 110 120 YPIYQTVNAG DGNALQNAIT TDGKGGSRHP QWFASQPRVV YIPPGTYTIS KTLRFNTDTI 130 140 150 160 170 180 LMGDPTNPPI IKAAAGFSGD QTLISAQDPS TNEKGELSFA VAIKNVVLDT TAIPGGNSFT 190 200 210 220 230 240 ALWWGVAQAA HLQNVRITMS SSSGGNGHTG IRMGRGSTLG LADVRVERGQ NGIWIDGHQQ 250 260 270 280 290 300 ASFHNIYFFQ NTIGMLISSG NTFSIFSSTF DTCGTAFPTL AGSPWIALID AKSINSGVTF 310 320 330 340 350 360 TTNQFPSFMI ENLTKDNGTP VVVVRGSTLV GASSHVNTYS YGNTVGRNPT YGDVTSSNTR 370 380 390 400 410 420 PSALAPGGRY PYVAPPTYGD LPISSFLNVK DPAQNGNRQV KGDNTINEAD TLNAILELAA 430 440 450 460 470 480 SQNKVAYFPF GKYRVDSTLF IPKGSRIVGE AWATITGNGN FFKNENSPQP VVSVGRAGDV 490 500 510 520 530 540 GIAQLQDLRV TTNDVLPGAI LVQFNMAGNN PGDVALWNSL VTVGGTRGAQ ALANACTNNS 550 560 570 580 590 600 NECKGAFIGI HVAKGSSPYI QNVWELGLRD HIAENFSGGT SHRRERWNFG PIRRNATCLY 610 620 630 640 650 660 PIGSGHWWLY QLNLHNAANV VVSLLQAETN YHQGANTQQI PPAPWVANVG TWGDPDFSWC 670 680 690 700 710 720 NGGDKRCRMG PANFINGGSN IYTYASAAWA FFSGPGQGCA QFECQQTIHW IASTPSNLQA 730 740 750 760 FGLCSKDSVN TLRLGDGTFI NTQNGYTGGW TPGGGDVARY TT

References

[1]

"A novel endo-beta-1,3-glucanase, BGN13.1, involved in the mycoparasitism of Trichoderma harzianum." de la Cruz J., Pintor-Toro J.A., Benitez T., Llobell A., Romero L.C. J. Bacteriol. 177:6937-6945(1995) [PubMed: 7592488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 48131 / CBS 354.33 / CECT 2413 / VTT D-80150.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X84085 mRNA. Translation: CAA58889.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH55. Glycoside Hydrolase Family 55.
Enzyme and pathway databases
BRENDA	3.2.1.39. 3745.
Family and domain databases
InterPro	IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13B_TRIHA

Accession

Primary (citable) accession number: P53626

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 24, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)