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P53625

- MA122_DROME

UniProt

P53625 - MA122_DROME

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Protein
Mannosyl-oligosaccharide alpha-1,2-mannosidase isoform B
Gene
alpha-Man-I, ALPHA-MAN-1, mas-1, CG32684
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Cofactori

Calcium By similarity.

Pathwayi

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. determination of adult lifespan Source: FlyBase
  2. encapsulation of foreign target Source: FlyBase
  3. protein glycosylation Source: UniProtKB-UniPathway
  4. response to anesthetic Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiREACT_180226. Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl-oligosaccharide alpha-1,2-mannosidase isoform B (EC:3.2.1.113)
Alternative name(s):
Man(9)-alpha-mannosidase
Mannosidase-1
Gene namesi
Name:alpha-Man-I
Synonyms:ALPHA-MAN-1, mas-1
ORF Names:CG32684
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome X

Organism-specific databases

FlyBaseiFBgn0259170. alpha-Man-I.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei12 – 3221Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini33 – 643611Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 643643Mannosyl-oligosaccharide alpha-1,2-mannosidase isoform B
PRO_0000210317Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi36 – 361N-linked (GlcNAc...) Reviewed prediction
Glycosylationi77 – 771N-linked (GlcNAc...) Reviewed prediction
Glycosylationi166 – 1661N-linked (GlcNAc...) Reviewed prediction
Glycosylationi254 – 2541N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Complex spatial distribution during embryogenesis, including expression in lobula plate giant neurons. Also expressed in adult wing and eyes.

Developmental stagei

Expressed both maternally and zygotically during embryonic stages.

Gene expression databases

BgeeiP53625.

Interactioni

Protein-protein interaction databases

BioGridi58390. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP53625.
SMRiP53625. Positions 158-623.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000016529.
InParanoidiP53625.
KOiK01230.
OMAiYFREENS.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERiPTHR11742. PTHR11742. 1 hit.
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform B (identifier: P53625-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MCPKTSKTTP LLLIGGICFV IVLVGITGIT LINNINLSNI IRLNEKVASD    50
SVSNNENQIK ELNYVNNHPR NVYLKLNASS RDDEDDEQMQ KEQEQLELPK 100
ISAVIGGSKP KVEDNQVKES SEVISPSTST FSMRSSAGEL TSTSAPLSSI 150
VSVTAPPMPF GGVKYNQSSG LIDYEKRNQV VKMMEHAWHN YKLYAWGKNE 200
LRPLSQRPHS ASIFGSYDLG ATIVDGLDTL YIMGLEKEYR EGRDWIERKF 250
SLDNISAELS VFETNIRFVG GMLTLYAFTG DPLYKEKAQH VADKLLPAFQ 300
TPTGIPYALV NTKTGVAKNY GWASGGSSIL SEFGTLHLEF AYLSDITGNP 350
LYRERVQTIR QVLKEIEKPK GLYPNFLNPK TGKWGQLHMS LGALGDSYYE 400
YLLKAWLQSG QTDEEAREMF DEAMLAILDK MVRTSPGGLT YVSDLKFDRL 450
EHKMDHLACF SGGLFALGAA TRQNDYTDKY MEVGKGITNT CHESYIRAPT 500
QLGPEAFRFS EAVEARALRS QEKYYILRPE TFESYFVLWR LTHDQKYRDW 550
GWEAVLALEK HCRTAHGYCG LRNVYQQEPQ KDDVQQSFFL AETLKYLYLL 600
FSDDSVLPLD EWVFNTEAHP LPIKGANAYY RQAPVTLPVS NAS 643
Length:643
Mass (Da):72,525
Last modified:March 15, 2004 - v2
Checksum:i8725B99D31DE7AC3
GO
Isoform A (identifier: P53624-1) [UniParc]FASTAAdd to Basket

Also known as: C, D, E, F, G

The sequence of this isoform can be found in the external entry P53624.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:667
Mass (Da):74,966
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti544 – 5441D → E in CAA57963. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X82641 mRNA. Translation: CAA57963.1.
AE014298 Genomic DNA. Translation: AAF46571.3.
PIRiS60710.
RefSeqiNP_001259402.1. NM_001272473.1. [P53625-1]
NP_511105.2. NM_078550.3. [P53625-1]
UniGeneiDm.7316.

Genome annotation databases

EnsemblMetazoaiFBtr0300512; FBpp0289739; FBgn0259170. [P53625-1]
FBtr0331759; FBpp0304147; FBgn0259170. [P53625-1]
GeneIDi31957.
KEGGidme:Dmel_CG42275.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X82641 mRNA. Translation: CAA57963.1 .
AE014298 Genomic DNA. Translation: AAF46571.3 .
PIRi S60710.
RefSeqi NP_001259402.1. NM_001272473.1. [P53625-1 ]
NP_511105.2. NM_078550.3. [P53625-1 ]
UniGenei Dm.7316.

3D structure databases

ProteinModelPortali P53625.
SMRi P53625. Positions 158-623.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 58390. 2 interactions.

Chemistry

BindingDBi P53625.
ChEMBLi CHEMBL1697673.

Protein family/group databases

CAZyi GH47. Glycoside Hydrolase Family 47.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0300512 ; FBpp0289739 ; FBgn0259170 . [P53625-1 ]
FBtr0331759 ; FBpp0304147 ; FBgn0259170 . [P53625-1 ]
GeneIDi 31957.
KEGGi dme:Dmel_CG42275.

Organism-specific databases

CTDi 31957.
FlyBasei FBgn0259170. alpha-Man-I.

Phylogenomic databases

GeneTreei ENSGT00390000016529.
InParanoidi P53625.
KOi K01230.
OMAi YFREENS.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_180226. Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2.

Miscellaneous databases

ChiTaRSi alpha-Man-I. drosophila.
GenomeRNAii 31957.
NextBioi 776122.

Gene expression databases

Bgeei P53625.

Family and domain databases

Gene3Di 1.50.10.50. 1 hit.
InterProi IPR001382. Glyco_hydro_47.
[Graphical view ]
PANTHERi PTHR11742. PTHR11742. 1 hit.
Pfami PF01532. Glyco_hydro_47. 1 hit.
[Graphical view ]
PRINTSi PR00747. GLYHDRLASE47.
SUPFAMi SSF48225. SSF48225. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular and genetic analysis of the Drosophila mas-1 (mannosidase-1) gene which encodes a glycoprotein processing alpha 1,2-mannosidase."
    Kerscher S., Albert S., Wucherpfennig D., Heisenberg M., Schneuwly S.
    Dev. Biol. 168:613-626(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Berlin.
    Tissue: Head.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.

Entry informationi

Entry nameiMA122_DROME
AccessioniPrimary (citable) accession number: P53625
Secondary accession number(s): Q9W2W6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 15, 2004
Last modified: September 3, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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