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Protein

Mannosyl-oligosaccharide alpha-1,2-mannosidase isoform B

Gene

alpha-Man-I

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Cofactori

Ca2+By similarity

Pathwayi

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. determination of adult lifespan Source: FlyBase
  2. encapsulation of foreign target Source: FlyBase
  3. protein glycosylation Source: UniProtKB-UniPathway
  4. response to anesthetic Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiREACT_283988. Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl-oligosaccharide alpha-1,2-mannosidase isoform B (EC:3.2.1.113)
Alternative name(s):
Man(9)-alpha-mannosidase
Mannosidase-1
Gene namesi
Name:alpha-Man-I
Synonyms:ALPHA-MAN-1, mas-1
ORF Names:CG32684
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0259170. alpha-Man-I.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei12 – 3221Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini33 – 643611LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endomembrane system Source: FlyBase
  2. Golgi membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 643643Mannosyl-oligosaccharide alpha-1,2-mannosidase isoform BPRO_0000210317Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi36 – 361N-linked (GlcNAc...)Sequence Analysis
Glycosylationi77 – 771N-linked (GlcNAc...)Sequence Analysis
Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis
Glycosylationi254 – 2541N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Complex spatial distribution during embryogenesis, including expression in lobula plate giant neurons. Also expressed in adult wing and eyes.

Developmental stagei

Expressed both maternally and zygotically during embryonic stages.

Gene expression databases

BgeeiP53625.

Interactioni

Protein-protein interaction databases

BioGridi58390. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP53625.
SMRiP53625. Positions 158-623.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000016529.
KOiK01230.
OMAiYFREENS.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERiPTHR11742. PTHR11742. 1 hit.
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B (identifier: P53625-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCPKTSKTTP LLLIGGICFV IVLVGITGIT LINNINLSNI IRLNEKVASD
60 70 80 90 100
SVSNNENQIK ELNYVNNHPR NVYLKLNASS RDDEDDEQMQ KEQEQLELPK
110 120 130 140 150
ISAVIGGSKP KVEDNQVKES SEVISPSTST FSMRSSAGEL TSTSAPLSSI
160 170 180 190 200
VSVTAPPMPF GGVKYNQSSG LIDYEKRNQV VKMMEHAWHN YKLYAWGKNE
210 220 230 240 250
LRPLSQRPHS ASIFGSYDLG ATIVDGLDTL YIMGLEKEYR EGRDWIERKF
260 270 280 290 300
SLDNISAELS VFETNIRFVG GMLTLYAFTG DPLYKEKAQH VADKLLPAFQ
310 320 330 340 350
TPTGIPYALV NTKTGVAKNY GWASGGSSIL SEFGTLHLEF AYLSDITGNP
360 370 380 390 400
LYRERVQTIR QVLKEIEKPK GLYPNFLNPK TGKWGQLHMS LGALGDSYYE
410 420 430 440 450
YLLKAWLQSG QTDEEAREMF DEAMLAILDK MVRTSPGGLT YVSDLKFDRL
460 470 480 490 500
EHKMDHLACF SGGLFALGAA TRQNDYTDKY MEVGKGITNT CHESYIRAPT
510 520 530 540 550
QLGPEAFRFS EAVEARALRS QEKYYILRPE TFESYFVLWR LTHDQKYRDW
560 570 580 590 600
GWEAVLALEK HCRTAHGYCG LRNVYQQEPQ KDDVQQSFFL AETLKYLYLL
610 620 630 640
FSDDSVLPLD EWVFNTEAHP LPIKGANAYY RQAPVTLPVS NAS
Length:643
Mass (Da):72,525
Last modified:March 14, 2004 - v2
Checksum:i8725B99D31DE7AC3
GO
Isoform A (identifier: P53624-1) [UniParc]FASTAAdd to basket

Also known as: C, D, E, F, G

The sequence of this isoform can be found in the external entry P53624.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:667
Mass (Da):74,966
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti544 – 5441D → E in CAA57963 (PubMed:7729592).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82641 mRNA. Translation: CAA57963.1.
AE014298 Genomic DNA. Translation: AAF46571.3.
PIRiS60710.
RefSeqiNP_001259402.1. NM_001272473.2. [P53625-1]
NP_511105.2. NM_078550.4. [P53625-1]
UniGeneiDm.7316.

Genome annotation databases

EnsemblMetazoaiFBtr0300512; FBpp0289739; FBgn0259170. [P53625-1]
FBtr0331759; FBpp0304147; FBgn0259170. [P53625-1]
GeneIDi31957.
KEGGidme:Dmel_CG42275.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82641 mRNA. Translation: CAA57963.1.
AE014298 Genomic DNA. Translation: AAF46571.3.
PIRiS60710.
RefSeqiNP_001259402.1. NM_001272473.2. [P53625-1]
NP_511105.2. NM_078550.4. [P53625-1]
UniGeneiDm.7316.

3D structure databases

ProteinModelPortaliP53625.
SMRiP53625. Positions 158-623.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi58390. 2 interactions.

Chemistry

BindingDBiP53625.
ChEMBLiCHEMBL1697673.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0300512; FBpp0289739; FBgn0259170. [P53625-1]
FBtr0331759; FBpp0304147; FBgn0259170. [P53625-1]
GeneIDi31957.
KEGGidme:Dmel_CG42275.

Organism-specific databases

CTDi31957.
FlyBaseiFBgn0259170. alpha-Man-I.

Phylogenomic databases

GeneTreeiENSGT00390000016529.
KOiK01230.
OMAiYFREENS.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_283988. Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2.

Miscellaneous databases

ChiTaRSialpha-Man-I. fly.
GenomeRNAii31957.
NextBioi776122.

Gene expression databases

BgeeiP53625.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERiPTHR11742. PTHR11742. 1 hit.
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular and genetic analysis of the Drosophila mas-1 (mannosidase-1) gene which encodes a glycoprotein processing alpha 1,2-mannosidase."
    Kerscher S., Albert S., Wucherpfennig D., Heisenberg M., Schneuwly S.
    Dev. Biol. 168:613-626(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Berlin.
    Tissue: Head.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.

Entry informationi

Entry nameiMA122_DROME
AccessioniPrimary (citable) accession number: P53625
Secondary accession number(s): Q9W2W6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1996
Last sequence update: March 14, 2004
Last modified: March 31, 2015
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.