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P53625 (MA122_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mannosyl-oligosaccharide alpha-1,2-mannosidase isoform B

EC=3.2.1.113
Alternative name(s):
Man(9)-alpha-mannosidase
Mannosidase-1
Gene names
Name:alpha-Man-I
Synonyms:ALPHA-MAN-1, mas-1
ORF Names:CG32684
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length643 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.

Catalytic activity

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Cofactor

Calcium By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein.

Tissue specificity

Complex spatial distribution during embryogenesis, including expression in lobula plate giant neurons. Also expressed in adult wing and eyes.

Developmental stage

Expressed both maternally and zygotically during embryonic stages.

Sequence similarities

Belongs to the glycosyl hydrolase 47 family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform B (identifier: P53625-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform A (identifier: P53624-1)

Also known as: C; D; E; F; G;

The sequence of this isoform can be found in the external entry P53624.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 643643Mannosyl-oligosaccharide alpha-1,2-mannosidase isoform B
PRO_0000210317

Regions

Topological domain1 – 1111Cytoplasmic Potential
Transmembrane12 – 3221Helical; Signal-anchor for type II membrane protein; Potential
Topological domain33 – 643611Lumenal Potential

Amino acid modifications

Glycosylation361N-linked (GlcNAc...) Potential
Glycosylation771N-linked (GlcNAc...) Potential
Glycosylation1661N-linked (GlcNAc...) Potential
Glycosylation2541N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict5441D → E in CAA57963. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform B [UniParc].

Last modified March 15, 2004. Version 2.
Checksum: 8725B99D31DE7AC3

FASTA64372,525
        10         20         30         40         50         60 
MCPKTSKTTP LLLIGGICFV IVLVGITGIT LINNINLSNI IRLNEKVASD SVSNNENQIK 

        70         80         90        100        110        120 
ELNYVNNHPR NVYLKLNASS RDDEDDEQMQ KEQEQLELPK ISAVIGGSKP KVEDNQVKES 

       130        140        150        160        170        180 
SEVISPSTST FSMRSSAGEL TSTSAPLSSI VSVTAPPMPF GGVKYNQSSG LIDYEKRNQV 

       190        200        210        220        230        240 
VKMMEHAWHN YKLYAWGKNE LRPLSQRPHS ASIFGSYDLG ATIVDGLDTL YIMGLEKEYR 

       250        260        270        280        290        300 
EGRDWIERKF SLDNISAELS VFETNIRFVG GMLTLYAFTG DPLYKEKAQH VADKLLPAFQ 

       310        320        330        340        350        360 
TPTGIPYALV NTKTGVAKNY GWASGGSSIL SEFGTLHLEF AYLSDITGNP LYRERVQTIR 

       370        380        390        400        410        420 
QVLKEIEKPK GLYPNFLNPK TGKWGQLHMS LGALGDSYYE YLLKAWLQSG QTDEEAREMF 

       430        440        450        460        470        480 
DEAMLAILDK MVRTSPGGLT YVSDLKFDRL EHKMDHLACF SGGLFALGAA TRQNDYTDKY 

       490        500        510        520        530        540 
MEVGKGITNT CHESYIRAPT QLGPEAFRFS EAVEARALRS QEKYYILRPE TFESYFVLWR 

       550        560        570        580        590        600 
LTHDQKYRDW GWEAVLALEK HCRTAHGYCG LRNVYQQEPQ KDDVQQSFFL AETLKYLYLL 

       610        620        630        640 
FSDDSVLPLD EWVFNTEAHP LPIKGANAYY RQAPVTLPVS NAS 

« Hide

Isoform A (C) (D) (E) (F) (G) [UniParc].

See P53624.

References

« Hide 'large scale' references
[1]"Molecular and genetic analysis of the Drosophila mas-1 (mannosidase-1) gene which encodes a glycoprotein processing alpha 1,2-mannosidase."
Kerscher S., Albert S., Wucherpfennig D., Heisenberg M., Schneuwly S.
Dev. Biol. 168:613-626(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Berlin.
Tissue: Head.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X82641 mRNA. Translation: CAA57963.1.
AE014298 Genomic DNA. Translation: AAF46571.3.
PIRS60710.
RefSeqNP_001259402.1. NM_001272473.1.
NP_511105.2. NM_078550.3.
UniGeneDm.7316.

3D structure databases

ProteinModelPortalP53625.
SMRP53625. Positions 158-623.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid58390. 2 interactions.

Chemistry

BindingDBP53625.
ChEMBLCHEMBL1697673.

Protein family/group databases

CAZyGH47. Glycoside Hydrolase Family 47.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0300512; FBpp0289739; FBgn0259170. [P53625-1]
FBtr0331759; FBpp0304147; FBgn0259170. [P53625-1]
GeneID31957.
KEGGdme:Dmel_CG42275.

Organism-specific databases

CTD31957.
FlyBaseFBgn0259170. alpha-Man-I.

Phylogenomic databases

GeneTreeENSGT00390000016529.
InParanoidP53625.
KOK01230.
OMAYFREENS.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

BgeeP53625.

Family and domain databases

Gene3D1.50.10.50. 1 hit.
InterProIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERPTHR11742. PTHR11742. 1 hit.
PfamPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSPR00747. GLYHDRLASE47.
SUPFAMSSF48225. SSF48225. 1 hit.
ProtoNetSearch...

Other

ChiTaRSalpha-Man-I. drosophila.
GenomeRNAi31957.
NextBio776122.

Entry information

Entry nameMA122_DROME
AccessionPrimary (citable) accession number: P53625
Secondary accession number(s): Q9W2W6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 15, 2004
Last modified: April 16, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase