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Protein

Mannosyl-oligosaccharide alpha-1,2-mannosidase IA

Gene

alpha-Man-Ia

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.By similarity

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.By similarity

Cofactori

Ca2+By similarity, Mg2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei529 – 5291Proton donorBy similarity
Metal bindingi640 – 6401CalciumBy similarity

GO - Molecular functioni

GO - Biological processi

  • determination of adult lifespan Source: FlyBase
  • encapsulation of foreign target Source: FlyBase
  • N-glycan processing Source: GO_Central
  • response to anesthetic Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-DME-964827. Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl-oligosaccharide alpha-1,2-mannosidase IAImported (EC:3.2.1.113By similarity)
Alternative name(s):
Man(9)-alpha-mannosidase
Mannosidase-1
Gene namesi
Name:alpha-Man-IaImported
Synonyms:alpha-man-1Imported, mas-1Imported
ORF Names:CG32684Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0259170. alpha-Man-Ia.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1818CytoplasmicSequence analysisAdd
BLAST
Transmembranei19 – 3921Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini40 – 667628LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 667667Mannosyl-oligosaccharide alpha-1,2-mannosidase IAPRO_0000210316Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi278 – 2781N-linked (GlcNAc...)Sequence analysis
Disulfide bondi483 ↔ 515By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Complex spatial distribution during embryogenesis, including expression in lobula plate giant neurons. Also expressed in adult wing and eyes.1 Publication

Developmental stagei

Expressed both maternally and zygotically during embryonic stages.1 Publication

Gene expression databases

BgeeiP53624.
GenevisibleiP53624. DM.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
mbmQ9VPM51EBI-179043,EBI-178893
stepQ9V9Q61EBI-179043,EBI-164677

Protein-protein interaction databases

BioGridi58390. 2 interactions.
IntActiP53624. 2 interactions.
MINTiMINT-946839.

Structurei

3D structure databases

ProteinModelPortaliP53624.
SMRiP53624. Positions 194-647.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.UniRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000016529.
InParanoidiP53624.
OMAiHEAREMY.
OrthoDBiEOG7X0VH1.
PhylomeDBiP53624.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERiPTHR11742. PTHR11742. 1 hit.
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

Isoform HImported (identifier: P53624-1) [UniParc]FASTAAdd to basket

Also known as: A1 Publication

, JImported, LImported, MImported, NImported, OImported

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYRISPIGRK SNFHSREKCL IGLVLVTLCF LCFGGIFLLP DNFGSDRVLR
60 70 80 90 100
VYKHFRKAGP EIFIPAPPLA AHAPHRSEDP HFIGDRQRLE QKIRAELGDM
110 120 130 140 150
LDEPPAAGGG EPGQFQVLAQ QAQAPAPVAA LADQPLDQDE GHAAIPVLAA
160 170 180 190 200
PVQGDNAASQ ASSHPQSSAQ QHNQQQPQLP LGGGGNDQAP DTLDATLEER
210 220 230 240 250
RQKVKEMMEH AWHNYKLYAW GKNELRPLSQ RPHSASIFGS YDLGATIVDG
260 270 280 290 300
LDTLYIMGLE KEYREGRDWI ERKFSLDNIS AELSVFETNI RFVGGMLTLY
310 320 330 340 350
AFTGDPLYKE KAQHVADKLL PAFQTPTGIP YALVNTKTGV AKNYGWASGG
360 370 380 390 400
SSILSEFGTL HLEFAYLSDI TGNPLYRERV QTIRQVLKEI EKPKGLYPNF
410 420 430 440 450
LNPKTGKWGQ LHMSLGALGD SYYEYLLKAW LQSGQTDEEA REMFDEAMLA
460 470 480 490 500
ILDKMVRTSP GGLTYVSDLK FDRLEHKMDH LACFSGGLFA LGAATRQNDY
510 520 530 540 550
TDKYMEVGKG ITNTCHESYI RAPTQLGPEA FRFSEAVEAR ALRSQEKYYI
560 570 580 590 600
LRPETFESYF VLWRLTHDQK YRDWGWEAVL ALEKHCRTAH GYCGLRNVYQ
610 620 630 640 650
QEPQKDDVQQ SFFLAETLKY LYLLFSDDSV LPLDEWVFNT EAHPLPIKGA
660
NAYYRQAPVT LPVSNAS
Length:667
Mass (Da):74,966
Last modified:March 15, 2004 - v2
Checksum:iD707F491E4AE47F2
GO
Isoform KImported (identifier: P53624-2) [UniParc] [UniParc]FASTAAdd to basket

Also known as: B1 Publication

, PImported

The sequence of this isoform differs from the canonical sequence as follows:
     1-206: MYRISPIGRK...LEERRQKVKE → MCPKTSKTTP...DYEKRNQVVK

Show »
Length:643
Mass (Da):72,525
Checksum:i8725B99D31DE7AC3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti21 – 211I → T in CAA57962 (PubMed:7729592).Curated
Sequence conflicti568 – 5681D → E in CAA57962 (PubMed:7729592).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 206206MYRIS…QKVKE → MCPKTSKTTPLLLIGGICFV IVLVGITGITLINNINLSNI IRLNEKVASDSVSNNENQIK ELNYVNNHPRNVYLKLNASS RDDEDDEQMQKEQEQLELPK ISAVIGGSKPKVEDNQVKES SEVISPSTSTFSMRSSAGEL TSTSAPLSSIVSVTAPPMPF GGVKYNQSSGLIDYEKRNQV VK in isoform K. VSP_057904Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82640 mRNA. Translation: CAA57962.1.
X82641 mRNA. Translation: CAA57963.1.
AE014298 Genomic DNA. Translation: AAF46570.1.
AE014298 Genomic DNA. Translation: AAF46571.3.
AE014298 Genomic DNA. Translation: AAS65302.1.
AE014298 Genomic DNA. Translation: AAS65303.1.
AE014298 Genomic DNA. Translation: AAS65304.1.
AE014298 Genomic DNA. Translation: AAS65305.1.
AE014298 Genomic DNA. Translation: AAS65306.1.
AE014298 Genomic DNA. Translation: AGB95245.1.
BT011036 mRNA. Translation: AAR30196.1.
PIRiS60709.
RefSeqiNP_001259402.1. NM_001272473.2. [P53624-2]
NP_511105.2. NM_078550.4. [P53624-2]
NP_727407.1. NM_167223.3. [P53624-1]
NP_996395.1. NM_206672.3. [P53624-1]
NP_996396.1. NM_206673.3. [P53624-1]
NP_996397.1. NM_206674.3. [P53624-1]
NP_996398.1. NM_206675.3. [P53624-1]
NP_996399.1. NM_206676.3. [P53624-1]
UniGeneiDm.7316.

Genome annotation databases

EnsemblMetazoaiFBtr0299632; FBpp0288907; FBgn0259170. [P53624-1]
FBtr0300511; FBpp0289738; FBgn0259170. [P53624-1]
FBtr0300512; FBpp0289739; FBgn0259170. [P53624-2]
FBtr0300513; FBpp0289740; FBgn0259170. [P53624-1]
FBtr0300514; FBpp0289741; FBgn0259170. [P53624-1]
FBtr0300515; FBpp0289742; FBgn0259170. [P53624-1]
FBtr0300516; FBpp0289743; FBgn0259170. [P53624-1]
FBtr0331759; FBpp0304147; FBgn0259170. [P53624-2]
GeneIDi31957.

Keywords - Coding sequence diversityi

Alternative promoter usage

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82640 mRNA. Translation: CAA57962.1.
X82641 mRNA. Translation: CAA57963.1.
AE014298 Genomic DNA. Translation: AAF46570.1.
AE014298 Genomic DNA. Translation: AAF46571.3.
AE014298 Genomic DNA. Translation: AAS65302.1.
AE014298 Genomic DNA. Translation: AAS65303.1.
AE014298 Genomic DNA. Translation: AAS65304.1.
AE014298 Genomic DNA. Translation: AAS65305.1.
AE014298 Genomic DNA. Translation: AAS65306.1.
AE014298 Genomic DNA. Translation: AGB95245.1.
BT011036 mRNA. Translation: AAR30196.1.
PIRiS60709.
RefSeqiNP_001259402.1. NM_001272473.2. [P53624-2]
NP_511105.2. NM_078550.4. [P53624-2]
NP_727407.1. NM_167223.3. [P53624-1]
NP_996395.1. NM_206672.3. [P53624-1]
NP_996396.1. NM_206673.3. [P53624-1]
NP_996397.1. NM_206674.3. [P53624-1]
NP_996398.1. NM_206675.3. [P53624-1]
NP_996399.1. NM_206676.3. [P53624-1]
UniGeneiDm.7316.

3D structure databases

ProteinModelPortaliP53624.
SMRiP53624. Positions 194-647.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi58390. 2 interactions.
IntActiP53624. 2 interactions.
MINTiMINT-946839.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0299632; FBpp0288907; FBgn0259170. [P53624-1]
FBtr0300511; FBpp0289738; FBgn0259170. [P53624-1]
FBtr0300512; FBpp0289739; FBgn0259170. [P53624-2]
FBtr0300513; FBpp0289740; FBgn0259170. [P53624-1]
FBtr0300514; FBpp0289741; FBgn0259170. [P53624-1]
FBtr0300515; FBpp0289742; FBgn0259170. [P53624-1]
FBtr0300516; FBpp0289743; FBgn0259170. [P53624-1]
FBtr0331759; FBpp0304147; FBgn0259170. [P53624-2]
GeneIDi31957.

Organism-specific databases

CTDi31957.
FlyBaseiFBgn0259170. alpha-Man-Ia.

Phylogenomic databases

GeneTreeiENSGT00390000016529.
InParanoidiP53624.
OMAiHEAREMY.
OrthoDBiEOG7X0VH1.
PhylomeDBiP53624.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-DME-964827. Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2.

Miscellaneous databases

ChiTaRSialpha-Man-I. fly.
GenomeRNAii31957.
NextBioi776122.

Gene expression databases

BgeeiP53624.
GenevisibleiP53624. DM.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERiPTHR11742. PTHR11742. 1 hit.
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular and genetic analysis of the Drosophila mas-1 (mannosidase-1) gene which encodes a glycoprotein processing alpha 1,2-mannosidase."
    Kerscher S., Albert S., Wucherpfennig D., Heisenberg M., Schneuwly S.
    Dev. Biol. 168:613-626(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS H AND K), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Berlin.
    Tissue: Head1 Publication and Ovary1 Publication.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiMA1A1_DROME
AccessioniPrimary (citable) accession number: P53624
Secondary accession number(s): A4V480
, M9PH54, P53625, Q9W2W6, Q9W2W7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 15, 2004
Last modified: January 20, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.