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P53624

- MA121_DROME

UniProt

P53624 - MA121_DROME

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Protein

Mannosyl-oligosaccharide alpha-1,2-mannosidase isoform A

Gene

alpha-Man-I

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Cofactori

Ca2+By similarity

Pathwayi

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. determination of adult lifespan Source: FlyBase
  2. encapsulation of foreign target Source: FlyBase
  3. protein glycosylation Source: UniProtKB-UniPathway
  4. response to anesthetic Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiREACT_180226. Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl-oligosaccharide alpha-1,2-mannosidase isoform A (EC:3.2.1.113)
Alternative name(s):
Man(9)-alpha-mannosidase
Mannosidase-1
Gene namesi
Name:alpha-Man-I
Synonyms:ALPHA-MAN-1, mas-1
ORF Names:CG32684
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome X

Organism-specific databases

FlyBaseiFBgn0259170. alpha-Man-I.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1818CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei19 – 3921Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini40 – 667628LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 667667Mannosyl-oligosaccharide alpha-1,2-mannosidase isoform APRO_0000210316Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi278 – 2781N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP53624.

Expressioni

Tissue specificityi

Complex spatial distribution during embryogenesis, including expression in lobula plate giant neurons. Also expressed in adult wing and eyes.

Developmental stagei

Expressed both maternally and zygotically during embryonic stages.

Gene expression databases

BgeeiP53624.
ExpressionAtlasiP53624. differential.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
mbmQ9VPM51EBI-179043,EBI-178893
stepQ9V9Q61EBI-179043,EBI-164677

Protein-protein interaction databases

BioGridi58390. 2 interactions.
IntActiP53624. 2 interactions.
MINTiMINT-946839.

Structurei

3D structure databases

ProteinModelPortaliP53624.
SMRiP53624. Positions 194-647.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG300315.
GeneTreeiENSGT00390000016529.
InParanoidiP53624.
KOiK01230.
OMAiYLIKSYV.
OrthoDBiEOG7X0VH1.
PhylomeDBiP53624.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERiPTHR11742. PTHR11742. 1 hit.
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform A (identifier: P53624-1) [UniParc]FASTAAdd to Basket

Also known as: C, D, E, F, G

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYRISPIGRK SNFHSREKCL IGLVLVTLCF LCFGGIFLLP DNFGSDRVLR
60 70 80 90 100
VYKHFRKAGP EIFIPAPPLA AHAPHRSEDP HFIGDRQRLE QKIRAELGDM
110 120 130 140 150
LDEPPAAGGG EPGQFQVLAQ QAQAPAPVAA LADQPLDQDE GHAAIPVLAA
160 170 180 190 200
PVQGDNAASQ ASSHPQSSAQ QHNQQQPQLP LGGGGNDQAP DTLDATLEER
210 220 230 240 250
RQKVKEMMEH AWHNYKLYAW GKNELRPLSQ RPHSASIFGS YDLGATIVDG
260 270 280 290 300
LDTLYIMGLE KEYREGRDWI ERKFSLDNIS AELSVFETNI RFVGGMLTLY
310 320 330 340 350
AFTGDPLYKE KAQHVADKLL PAFQTPTGIP YALVNTKTGV AKNYGWASGG
360 370 380 390 400
SSILSEFGTL HLEFAYLSDI TGNPLYRERV QTIRQVLKEI EKPKGLYPNF
410 420 430 440 450
LNPKTGKWGQ LHMSLGALGD SYYEYLLKAW LQSGQTDEEA REMFDEAMLA
460 470 480 490 500
ILDKMVRTSP GGLTYVSDLK FDRLEHKMDH LACFSGGLFA LGAATRQNDY
510 520 530 540 550
TDKYMEVGKG ITNTCHESYI RAPTQLGPEA FRFSEAVEAR ALRSQEKYYI
560 570 580 590 600
LRPETFESYF VLWRLTHDQK YRDWGWEAVL ALEKHCRTAH GYCGLRNVYQ
610 620 630 640 650
QEPQKDDVQQ SFFLAETLKY LYLLFSDDSV LPLDEWVFNT EAHPLPIKGA
660
NAYYRQAPVT LPVSNAS
Length:667
Mass (Da):74,966
Last modified:March 15, 2004 - v2
Checksum:iD707F491E4AE47F2
GO
Isoform B (identifier: P53625-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry P53625.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:643
Mass (Da):72,525
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti21 – 211I → T in CAA57962. (PubMed:7729592)Curated
Sequence conflicti568 – 5681D → E in CAA57962. (PubMed:7729592)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82640 mRNA. Translation: CAA57962.1.
AE014298 Genomic DNA. Translation: AAF46570.1.
AE014298 Genomic DNA. Translation: AAS65302.1.
AE014298 Genomic DNA. Translation: AAS65303.1.
AE014298 Genomic DNA. Translation: AAS65304.1.
AE014298 Genomic DNA. Translation: AAS65305.1.
AE014298 Genomic DNA. Translation: AAS65306.1.
BT011036 mRNA. Translation: AAR30196.1.
PIRiS60709.
RefSeqiNP_727407.1. NM_167223.3. [P53624-1]
NP_996395.1. NM_206672.3. [P53624-1]
NP_996396.1. NM_206673.3. [P53624-1]
NP_996397.1. NM_206674.3. [P53624-1]
NP_996398.1. NM_206675.3. [P53624-1]
NP_996399.1. NM_206676.3. [P53624-1]
UniGeneiDm.7316.

Genome annotation databases

EnsemblMetazoaiFBtr0299632; FBpp0288907; FBgn0259170. [P53624-1]
FBtr0300511; FBpp0289738; FBgn0259170. [P53624-1]
FBtr0300513; FBpp0289740; FBgn0259170. [P53624-1]
FBtr0300514; FBpp0289741; FBgn0259170. [P53624-1]
FBtr0300515; FBpp0289742; FBgn0259170. [P53624-1]
FBtr0300516; FBpp0289743; FBgn0259170. [P53624-1]
GeneIDi31957.
KEGGidme:Dmel_CG42275.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82640 mRNA. Translation: CAA57962.1 .
AE014298 Genomic DNA. Translation: AAF46570.1 .
AE014298 Genomic DNA. Translation: AAS65302.1 .
AE014298 Genomic DNA. Translation: AAS65303.1 .
AE014298 Genomic DNA. Translation: AAS65304.1 .
AE014298 Genomic DNA. Translation: AAS65305.1 .
AE014298 Genomic DNA. Translation: AAS65306.1 .
BT011036 mRNA. Translation: AAR30196.1 .
PIRi S60709.
RefSeqi NP_727407.1. NM_167223.3. [P53624-1 ]
NP_996395.1. NM_206672.3. [P53624-1 ]
NP_996396.1. NM_206673.3. [P53624-1 ]
NP_996397.1. NM_206674.3. [P53624-1 ]
NP_996398.1. NM_206675.3. [P53624-1 ]
NP_996399.1. NM_206676.3. [P53624-1 ]
UniGenei Dm.7316.

3D structure databases

ProteinModelPortali P53624.
SMRi P53624. Positions 194-647.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 58390. 2 interactions.
IntActi P53624. 2 interactions.
MINTi MINT-946839.

Protein family/group databases

CAZyi GH47. Glycoside Hydrolase Family 47.

Proteomic databases

PaxDbi P53624.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0299632 ; FBpp0288907 ; FBgn0259170 . [P53624-1 ]
FBtr0300511 ; FBpp0289738 ; FBgn0259170 . [P53624-1 ]
FBtr0300513 ; FBpp0289740 ; FBgn0259170 . [P53624-1 ]
FBtr0300514 ; FBpp0289741 ; FBgn0259170 . [P53624-1 ]
FBtr0300515 ; FBpp0289742 ; FBgn0259170 . [P53624-1 ]
FBtr0300516 ; FBpp0289743 ; FBgn0259170 . [P53624-1 ]
GeneIDi 31957.
KEGGi dme:Dmel_CG42275.

Organism-specific databases

CTDi 31957.
FlyBasei FBgn0259170. alpha-Man-I.

Phylogenomic databases

eggNOGi NOG300315.
GeneTreei ENSGT00390000016529.
InParanoidi P53624.
KOi K01230.
OMAi YLIKSYV.
OrthoDBi EOG7X0VH1.
PhylomeDBi P53624.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_180226. Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2.

Miscellaneous databases

ChiTaRSi alpha-Man-I. fly.
GenomeRNAii 31957.
NextBioi 776122.

Gene expression databases

Bgeei P53624.
ExpressionAtlasi P53624. differential.

Family and domain databases

Gene3Di 1.50.10.50. 1 hit.
InterProi IPR001382. Glyco_hydro_47.
[Graphical view ]
PANTHERi PTHR11742. PTHR11742. 1 hit.
Pfami PF01532. Glyco_hydro_47. 1 hit.
[Graphical view ]
PRINTSi PR00747. GLYHDRLASE47.
SUPFAMi SSF48225. SSF48225. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular and genetic analysis of the Drosophila mas-1 (mannosidase-1) gene which encodes a glycoprotein processing alpha 1,2-mannosidase."
    Kerscher S., Albert S., Wucherpfennig D., Heisenberg M., Schneuwly S.
    Dev. Biol. 168:613-626(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Berlin.
    Tissue: Ovary.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiMA121_DROME
AccessioniPrimary (citable) accession number: P53624
Secondary accession number(s): A4V480, Q9W2W7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 15, 2004
Last modified: November 26, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3