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P53622

- COPA_YEAST

UniProt

P53622 - COPA_YEAST

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Protein

Coatomer subunit alpha

Gene
COP1, RET1, SEC33, SOO1, YDL145C, D1578
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.1 Publication

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. structural molecule activity Source: InterPro
  3. ubiquitin binding Source: SGD

GO - Biological processi

  1. ER to Golgi vesicle-mediated transport Source: SGD
  2. intracellular protein transport Source: InterPro
  3. retrograde vesicle-mediated transport, Golgi to ER Source: SGD
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29542-MONOMER.
ReactomeiREACT_33101. COPI Mediated Transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Coatomer subunit alpha
Alternative name(s):
Alpha-coat protein
Short name:
Alpha-COP
Retrieval from endoplasmic reticulum protein 1
Secretory protein 22
Suppressor of osmo-sensitivity 1
Gene namesi
Name:COP1
Synonyms:RET1, SEC33, SOO1
Ordered Locus Names:YDL145C
ORF Names:D1578
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDL145c.
SGDiS000002304. COP1.

Subcellular locationi

Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicleCOPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side
Note: The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.3 Publications

GO - Cellular componenti

  1. COPI vesicle coat Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12011201Coatomer subunit alphaPRO_0000050910Add
BLAST

Proteomic databases

MaxQBiP53622.
PaxDbiP53622.
PeptideAtlasiP53622.

Expressioni

Gene expression databases

GenevestigatoriP53622.

Interactioni

Subunit structurei

Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Interacts with the ESCRT-0 subunit VPS27. Interacts with KEI1 (via C-terminal region).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SEC27P4181117EBI-4860,EBI-4898
SEC28P405095EBI-4860,EBI-4884
WBP1P337673EBI-4860,EBI-12658

Protein-protein interaction databases

BioGridi31916. 164 interactions.
DIPiDIP-2582N.
IntActiP53622. 37 interactions.
MINTiMINT-422687.
STRINGi4932.YDL145C.

Structurei

Secondary structure

1
1201
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi649 – 65810
Helixi662 – 67211
Helixi675 – 68713
Helixi691 – 70010
Helixi704 – 71411
Helixi717 – 72913
Helixi733 – 74311
Helixi746 – 75510
Helixi759 – 76810
Helixi772 – 78110
Helixi786 – 7883
Helixi900 – 9078
Helixi911 – 9166
Helixi920 – 93112
Helixi937 – 9393
Helixi940 – 94910
Beta strandi951 – 9544
Beta strandi957 – 9615
Beta strandi964 – 9674
Beta strandi969 – 9713
Beta strandi973 – 9753
Helixi977 – 9793
Helixi987 – 100216
Helixi1006 – 102116
Beta strandi1025 – 10273
Helixi1029 – 105527
Turni1059 – 10613
Helixi1062 – 107110
Helixi1072 – 10743
Helixi1079 – 109517
Helixi1099 – 111012
Helixi1117 – 113014
Beta strandi1147 – 11493
Turni1151 – 11533
Beta strandi1156 – 11616
Beta strandi1163 – 11653
Turni1167 – 11693
Beta strandi1172 – 11743
Helixi1175 – 11773
Beta strandi1179 – 11813
Beta strandi1183 – 11864
Beta strandi1188 – 11914

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MKQX-ray2.50B/D/F642-818[»]
3MV2X-ray2.90A/C/E900-1201[»]
3MV3X-ray3.25A/C/E900-1201[»]
ProteinModelPortaliP53622.
SMRiP53622. Positions 1-818, 899-1201.

Miscellaneous databases

EvolutionaryTraceiP53622.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati9 – 3931WD 1Add
BLAST
Repeati51 – 8131WD 2Add
BLAST
Repeati93 – 12331WD 3Add
BLAST
Repeati135 – 16531WD 4Add
BLAST
Repeati207 – 23731WD 5Add
BLAST
Repeati251 – 28131WD 6Add
BLAST

Sequence similaritiesi

Contains 6 WD repeats.

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00520000055597.
HOGENOMiHOG000195913.
KOiK05236.
OMAiFWVLGAH.
OrthoDBiEOG7TN016.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR016391. Coatomer_asu.
IPR010714. Coatomer_asu_C.
IPR006692. Coatomer_WD-assoc_reg.
IPR020472. G-protein_beta_WD-40_rep.
IPR011044. Quino_amine_DH_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF04053. Coatomer_WDAD. 1 hit.
PF06957. COPI_C. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
PIRSFiPIRSF003354. Coatomer_alpha_subunit. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50969. SSF50969. 1 hit.
SSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53622-1 [UniParc]FASTAAdd to Basket

« Hide

MKMLTKFESK STRAKGIAFH PSRPWVLVAL FSSTIQLWDY RMGTLLHRFE     50
DHEGPVRGLD FHPTQPIFVS AGDDYTIKVW SLDTNKCLYT LTGHLDYVRT 100
VFFHRELPWI ISASDDQTIR IWNWQNRKEI ACLTGHNHFV MCAQFHPTDD 150
LIVSASLDET IRIWDISGLR KRHSAPGTSS FEEQMSAQQN LLDGSLGDCV 200
VKFILEGHTR GVNWASFHPT LPLIVSGSDD RQVKLWRMSA TKAWEVDTCR 250
GHTNNVDSVI FHPHQNLIIS VGEDKTLRVW DLDKRTPVKQ FKRENDRFWL 300
IAAHPHINLF GAAHDSGIMV FKLDRERPCS FIHQNQLFFV NAEKQIQSFN 350
FQKRVASLPY ASLKGIGQPW DAFRSISYNP SQHSVLVNEA NGKFALVILP 400
KQPVGAVEPT SVTQDTGNFA TFVGRNRFVV YNKNTESVEV RSLENKVTRN 450
IKVEETVRTI VAAGPGSVLV IHPREVILYD VQQGKKVSQL AVKNVKYVSW 500
SLDGQYVALM SKHTITLATK KLELINSMHE TIRIKSAAWD ETGVLIYSTL 550
NHIRYSLLNG DRGIIKTLEK TLYITKVQGK LVYCLNREGE IEILTIDPTE 600
YRFKKALVNK NFPEVLRLIK DSNLVGQNII SYLQKSGYPE IALQFVQDPH 650
IRFDLALEYG NLDVALDEAK KLNDSSTWER LIQEALAQGN ASLAEMIYQT 700
QHSFDKLSFL YLVTGDVNKL SKMQNIAQTR EDFGSMLLNT FYNNSTKERS 750
SIFAEGGSLP LAYAVAKANG DEAAASAFLE QAEVDEQDVT LPDQMDASNF 800
VQRPVISKPL EKWPLKEAEL SYFEKAVLGQ IDDLTIDDET PAVNTTQEQE 850
EPLGEENFND EDIGEDEGAW DLGDEDLDVG EELPEEVEQG EITSPAQEVE 900
TAIWIKNSKL PAVLVAAGAF DAAVQALSKQ VGVVKLEPLK KYFTNIYEGC 950
RTYIPSTPCE LPAQLGYVRA YDDTVSEDQI LPYVPGLDVV NEKMNEGYKN 1000
FKLNKPDIAI ECFREAIYRI TLLMVDDAED EKLAHKILET AREYILGLSI 1050
ELERRSLKEG NTVRMLELAA YFTKAKLSPI HRTNALQVAM SQHFKHKNFL 1100
QASYFAGEFL KIISSGPRAE QARKIKNKAD SMASDAIPID FDPYAKFDIC 1150
AATYKPIYED TPSVSDPLTG SKYVITEKDK IDRIAMISKI GAPASGLRIR 1200
V 1201
Length:1,201
Mass (Da):135,607
Last modified:November 1, 1997 - v2
Checksum:iD3589073106F5570
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti753 – 7531F → L in CAA86588. 1 Publication
Sequence conflicti753 – 7531F → L in CAA58712. 1 Publication
Sequence conflicti905 – 9051I → T in CAA86588. 1 Publication
Sequence conflicti905 – 9051I → T in CAA58712. 1 Publication
Sequence conflicti1006 – 10061P → L in CAA86588. 1 Publication
Sequence conflicti1006 – 10061P → L in CAA58712. 1 Publication
Sequence conflicti1027 – 10282DA → NT in CAA86588. 1 Publication
Sequence conflicti1027 – 10282DA → NT in CAA58712. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46617 Genomic DNA. Translation: CAA86588.1.
X83754 Genomic DNA. Translation: CAA58712.1.
Z74193 Genomic DNA. Translation: CAA98719.1.
Z74192 Genomic DNA. Translation: CAA98718.1.
X97751 Genomic DNA. Translation: CAA66346.1.
BK006938 Genomic DNA. Translation: DAA11713.1.
PIRiS67692. ERBYA.
RefSeqiNP_010136.1. NM_001180205.1.

Genome annotation databases

EnsemblFungiiYDL145C; YDL145C; YDL145C.
GeneIDi851410.
KEGGisce:YDL145C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46617 Genomic DNA. Translation: CAA86588.1 .
X83754 Genomic DNA. Translation: CAA58712.1 .
Z74193 Genomic DNA. Translation: CAA98719.1 .
Z74192 Genomic DNA. Translation: CAA98718.1 .
X97751 Genomic DNA. Translation: CAA66346.1 .
BK006938 Genomic DNA. Translation: DAA11713.1 .
PIRi S67692. ERBYA.
RefSeqi NP_010136.1. NM_001180205.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3MKQ X-ray 2.50 B/D/F 642-818 [» ]
3MV2 X-ray 2.90 A/C/E 900-1201 [» ]
3MV3 X-ray 3.25 A/C/E 900-1201 [» ]
ProteinModelPortali P53622.
SMRi P53622. Positions 1-818, 899-1201.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31916. 164 interactions.
DIPi DIP-2582N.
IntActi P53622. 37 interactions.
MINTi MINT-422687.
STRINGi 4932.YDL145C.

Proteomic databases

MaxQBi P53622.
PaxDbi P53622.
PeptideAtlasi P53622.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDL145C ; YDL145C ; YDL145C .
GeneIDi 851410.
KEGGi sce:YDL145C.

Organism-specific databases

CYGDi YDL145c.
SGDi S000002304. COP1.

Phylogenomic databases

eggNOGi COG2319.
GeneTreei ENSGT00520000055597.
HOGENOMi HOG000195913.
KOi K05236.
OMAi FWVLGAH.
OrthoDBi EOG7TN016.

Enzyme and pathway databases

BioCyci YEAST:G3O-29542-MONOMER.
Reactomei REACT_33101. COPI Mediated Transport.

Miscellaneous databases

EvolutionaryTracei P53622.
NextBioi 968591.
PROi P53622.

Gene expression databases

Genevestigatori P53622.

Family and domain databases

Gene3Di 2.130.10.10. 2 hits.
InterProi IPR016391. Coatomer_asu.
IPR010714. Coatomer_asu_C.
IPR006692. Coatomer_WD-assoc_reg.
IPR020472. G-protein_beta_WD-40_rep.
IPR011044. Quino_amine_DH_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF04053. Coatomer_WDAD. 1 hit.
PF06957. COPI_C. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view ]
PIRSFi PIRSF003354. Coatomer_alpha_subunit. 1 hit.
PRINTSi PR00320. GPROTEINBRPT.
SMARTi SM00320. WD40. 7 hits.
[Graphical view ]
SUPFAMi SSF50969. SSF50969. 1 hit.
SSF50978. SSF50978. 2 hits.
PROSITEi PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Coatomer is essential for retrieval of dilysine-tagged proteins to the endoplasmic reticulum."
    Letourneur F., Gaynor E.C., Hennecke S., Demolliere C., Durden R., Emr S.D., Riezman H., Cosson P.
    Cell 79:1199-1207(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-20.
    Strain: PC70.
  2. "Non-clathrin-coat protein alpha is a conserved subunit of coatomer and in Saccharomyces cerevisiae is essential for growth."
    Gerich B., Orci L., Tschochner H., Lottspeich F., Ravazolla M., Amherdt M., Wieland F., Harter C.
    Proc. Natl. Acad. Sci. U.S.A. 92:3229-3233(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-24, SUBCELLULAR LOCATION.
    Strain: RS453.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Analysis of a 23 kb region on the left arm of yeast chromosome IV."
    Delaveau T.T.D., Blugeon C., Jacq C., Perea J.
    Yeast 12:1587-1592(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 799-1201.
    Strain: ATCC 96604 / S288c / FY1679.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Involvement of specific COPI subunits in protein sorting from the late endosome to the vacuole in yeast."
    Gabriely G., Kama R., Gerst J.E.
    Mol. Cell. Biol. 27:526-540(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH VPS27.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Kei1: a novel subunit of inositolphosphorylceramide synthase, essential for its enzyme activity and Golgi localization."
    Sato K., Noda Y., Yoda K.
    Mol. Biol. Cell 20:4444-4457(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KEI1.
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCOPA_YEAST
AccessioniPrimary (citable) accession number: P53622
Secondary accession number(s): D6VRK3, Q07595
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: September 3, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 15200 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi