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Protein

Coatomer subunit alpha

Gene

COP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.1 Publication

Miscellaneous

Present with 15200 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • structural molecule activity Source: InterPro
  • ubiquitin binding Source: SGD

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: SGD
  • intracellular protein transport Source: GO_Central
  • intra-Golgi vesicle-mediated transport Source: GO_Central
  • retrograde vesicle-mediated transport, Golgi to ER Source: SGD

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29542-MONOMER
ReactomeiR-SCE-6807878 COPI-mediated anterograde transport
R-SCE-6811434 COPI-dependent Golgi-to-ER retrograde traffic

Names & Taxonomyi

Protein namesi
Recommended name:
Coatomer subunit alpha
Alternative name(s):
Alpha-coat protein
Short name:
Alpha-COP
Retrieval from endoplasmic reticulum protein 1
Secretory protein 22
Suppressor of osmo-sensitivity 1
Gene namesi
Name:COP1
Synonyms:RET1, SEC33, SOO1
Ordered Locus Names:YDL145C
ORF Names:D1578
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL145C
SGDiS000002304 COP1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000509101 – 1201Coatomer subunit alphaAdd BLAST1201

Proteomic databases

MaxQBiP53622
PaxDbiP53622
PRIDEiP53622

PTM databases

CarbonylDBiP53622
iPTMnetiP53622

Interactioni

Subunit structurei

Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Interacts with the ESCRT-0 subunit VPS27. Interacts with KEI1 (via C-terminal region).2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi31916, 488 interactors
DIPiDIP-2582N
IntActiP53622, 74 interactors
MINTiP53622
STRINGi4932.YDL145C

Structurei

Secondary structure

11201
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi649 – 658Combined sources10
Helixi662 – 672Combined sources11
Helixi675 – 687Combined sources13
Helixi691 – 700Combined sources10
Helixi704 – 714Combined sources11
Helixi717 – 729Combined sources13
Helixi733 – 743Combined sources11
Helixi746 – 755Combined sources10
Helixi759 – 768Combined sources10
Helixi772 – 781Combined sources10
Helixi786 – 788Combined sources3
Helixi900 – 907Combined sources8
Helixi911 – 916Combined sources6
Helixi920 – 931Combined sources12
Helixi937 – 939Combined sources3
Helixi940 – 949Combined sources10
Beta strandi951 – 954Combined sources4
Beta strandi957 – 961Combined sources5
Beta strandi964 – 967Combined sources4
Beta strandi969 – 971Combined sources3
Beta strandi973 – 975Combined sources3
Helixi977 – 979Combined sources3
Helixi987 – 1002Combined sources16
Helixi1006 – 1021Combined sources16
Beta strandi1025 – 1027Combined sources3
Helixi1029 – 1055Combined sources27
Turni1059 – 1061Combined sources3
Helixi1062 – 1071Combined sources10
Helixi1072 – 1074Combined sources3
Helixi1079 – 1095Combined sources17
Helixi1099 – 1110Combined sources12
Helixi1117 – 1130Combined sources14
Beta strandi1147 – 1149Combined sources3
Turni1151 – 1153Combined sources3
Beta strandi1156 – 1161Combined sources6
Beta strandi1163 – 1165Combined sources3
Turni1167 – 1169Combined sources3
Beta strandi1172 – 1174Combined sources3
Helixi1175 – 1177Combined sources3
Beta strandi1179 – 1181Combined sources3
Beta strandi1183 – 1186Combined sources4
Beta strandi1188 – 1191Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MKQX-ray2.50B/D/F642-818[»]
3MV2X-ray2.90A/C/E900-1201[»]
3MV3X-ray3.25A/C/E900-1201[»]
ProteinModelPortaliP53622
SMRiP53622
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53622

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati9 – 39WD 1Add BLAST31
Repeati51 – 81WD 2Add BLAST31
Repeati93 – 123WD 3Add BLAST31
Repeati135 – 165WD 4Add BLAST31
Repeati207 – 237WD 5Add BLAST31
Repeati251 – 281WD 6Add BLAST31

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00520000055597
HOGENOMiHOG000195913
InParanoidiP53622
KOiK05236
OMAiGHDNGVM
OrthoDBiEOG092C10HM

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR016391 Coatomer_asu
IPR010714 Coatomer_asu_C
IPR006692 Coatomer_WD-assoc_reg
IPR020472 G-protein_beta_WD-40_rep
IPR011044 Quino_amine_DH_bsu
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF04053 Coatomer_WDAD, 1 hit
PF06957 COPI_C, 1 hit
PF00400 WD40, 5 hits
PIRSFiPIRSF003354 Coatomer_alpha_subunit, 1 hit
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50969 SSF50969, 1 hit
SSF50978 SSF50978, 2 hits
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 2 hits
PS50082 WD_REPEATS_2, 5 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

P53622-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMLTKFESK STRAKGIAFH PSRPWVLVAL FSSTIQLWDY RMGTLLHRFE
60 70 80 90 100
DHEGPVRGLD FHPTQPIFVS AGDDYTIKVW SLDTNKCLYT LTGHLDYVRT
110 120 130 140 150
VFFHRELPWI ISASDDQTIR IWNWQNRKEI ACLTGHNHFV MCAQFHPTDD
160 170 180 190 200
LIVSASLDET IRIWDISGLR KRHSAPGTSS FEEQMSAQQN LLDGSLGDCV
210 220 230 240 250
VKFILEGHTR GVNWASFHPT LPLIVSGSDD RQVKLWRMSA TKAWEVDTCR
260 270 280 290 300
GHTNNVDSVI FHPHQNLIIS VGEDKTLRVW DLDKRTPVKQ FKRENDRFWL
310 320 330 340 350
IAAHPHINLF GAAHDSGIMV FKLDRERPCS FIHQNQLFFV NAEKQIQSFN
360 370 380 390 400
FQKRVASLPY ASLKGIGQPW DAFRSISYNP SQHSVLVNEA NGKFALVILP
410 420 430 440 450
KQPVGAVEPT SVTQDTGNFA TFVGRNRFVV YNKNTESVEV RSLENKVTRN
460 470 480 490 500
IKVEETVRTI VAAGPGSVLV IHPREVILYD VQQGKKVSQL AVKNVKYVSW
510 520 530 540 550
SLDGQYVALM SKHTITLATK KLELINSMHE TIRIKSAAWD ETGVLIYSTL
560 570 580 590 600
NHIRYSLLNG DRGIIKTLEK TLYITKVQGK LVYCLNREGE IEILTIDPTE
610 620 630 640 650
YRFKKALVNK NFPEVLRLIK DSNLVGQNII SYLQKSGYPE IALQFVQDPH
660 670 680 690 700
IRFDLALEYG NLDVALDEAK KLNDSSTWER LIQEALAQGN ASLAEMIYQT
710 720 730 740 750
QHSFDKLSFL YLVTGDVNKL SKMQNIAQTR EDFGSMLLNT FYNNSTKERS
760 770 780 790 800
SIFAEGGSLP LAYAVAKANG DEAAASAFLE QAEVDEQDVT LPDQMDASNF
810 820 830 840 850
VQRPVISKPL EKWPLKEAEL SYFEKAVLGQ IDDLTIDDET PAVNTTQEQE
860 870 880 890 900
EPLGEENFND EDIGEDEGAW DLGDEDLDVG EELPEEVEQG EITSPAQEVE
910 920 930 940 950
TAIWIKNSKL PAVLVAAGAF DAAVQALSKQ VGVVKLEPLK KYFTNIYEGC
960 970 980 990 1000
RTYIPSTPCE LPAQLGYVRA YDDTVSEDQI LPYVPGLDVV NEKMNEGYKN
1010 1020 1030 1040 1050
FKLNKPDIAI ECFREAIYRI TLLMVDDAED EKLAHKILET AREYILGLSI
1060 1070 1080 1090 1100
ELERRSLKEG NTVRMLELAA YFTKAKLSPI HRTNALQVAM SQHFKHKNFL
1110 1120 1130 1140 1150
QASYFAGEFL KIISSGPRAE QARKIKNKAD SMASDAIPID FDPYAKFDIC
1160 1170 1180 1190 1200
AATYKPIYED TPSVSDPLTG SKYVITEKDK IDRIAMISKI GAPASGLRIR

V
Length:1,201
Mass (Da):135,607
Last modified:November 1, 1997 - v2
Checksum:iD3589073106F5570
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti753F → L in CAA86588 (PubMed:8001155).Curated1
Sequence conflicti753F → L in CAA58712 (PubMed:7724544).Curated1
Sequence conflicti905I → T in CAA86588 (PubMed:8001155).Curated1
Sequence conflicti905I → T in CAA58712 (PubMed:7724544).Curated1
Sequence conflicti1006P → L in CAA86588 (PubMed:8001155).Curated1
Sequence conflicti1006P → L in CAA58712 (PubMed:7724544).Curated1
Sequence conflicti1027 – 1028DA → NT in CAA86588 (PubMed:8001155).Curated2
Sequence conflicti1027 – 1028DA → NT in CAA58712 (PubMed:7724544).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46617 Genomic DNA Translation: CAA86588.1
X83754 Genomic DNA Translation: CAA58712.1
Z74193 Genomic DNA Translation: CAA98719.1
Z74192 Genomic DNA Translation: CAA98718.1
X97751 Genomic DNA Translation: CAA66346.1
BK006938 Genomic DNA Translation: DAA11713.1
PIRiS67692 ERBYA
RefSeqiNP_010136.1, NM_001180205.1

Genome annotation databases

EnsemblFungiiYDL145C; YDL145C; YDL145C
GeneIDi851410
KEGGisce:YDL145C

Similar proteinsi

Entry informationi

Entry nameiCOPA_YEAST
AccessioniPrimary (citable) accession number: P53622
Secondary accession number(s): D6VRK3, Q07595
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 176 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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