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Protein

Coatomer subunit alpha

Gene

COPA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).By similarity
Xenin stimulates exocrine pancreatic secretion. It inhibits pentagastrin-stimulated secretion of acid, to induce exocrine pancreatic secretion and to affect small and large intestinal motility. In the gut, xenin interacts with the neurotensin receptor.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hormone

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000122218-MONOMER.
ReactomeiR-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
SignaLinkiP53621.

Names & Taxonomyi

Protein namesi
Recommended name:
Coatomer subunit alpha
Alternative name(s):
Alpha-coat protein
Short name:
Alpha-COP
HEP-COP
Short name:
HEPCOP
Cleaved into the following 2 chains:
Alternative name(s):
Xenopsin-related peptide
Gene namesi
Name:COPA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:2230. COPA.

Subcellular locationi

Xenin :

GO - Cellular componenti

  • COPI vesicle coat Source: UniProtKB
  • cytoplasm Source: MGI
  • cytosol Source: Reactome
  • endoplasmic reticulum membrane Source: Reactome
  • extracellular exosome Source: UniProtKB
  • extracellular space Source: MGI
  • Golgi membrane Source: Reactome
  • membrane Source: UniProtKB
  • transport vesicle Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Involvement in diseasei

Autoimmune interstitial lung, joint, and kidney disease (AILJK)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionAn autoimmune disease characterized by inflammatory arthritis, interstitial lung disease, and immune complex-mediated renal disease.
See also OMIM:616414
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_073844230K → N in AILJK; causes a defect in retrograde transport from the Golgi to the endoplasmic reticulum. 1 Publication1
Natural variantiVAR_073845233R → H in AILJK. 1 PublicationCorresponds to variant rs794727993dbSNPEnsembl.1
Natural variantiVAR_073846241E → K in AILJK; causes a defect in retrograde transport from the Golgi to the endoplasmic reticulum. 1 PublicationCorresponds to variant rs794727995dbSNPEnsembl.1
Natural variantiVAR_073847243D → G in AILJK. 1 PublicationCorresponds to variant rs794727994dbSNPEnsembl.1

Keywords - Diseasei

Disease mutation

Organism-specific databases

DisGeNETi1314.
MIMi616414. phenotype.
OpenTargetsiENSG00000122218.
PharmGKBiPA26746.

Polymorphism and mutation databases

BioMutaiCOPA.
DMDMi205371746.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002233071 – 1224Coatomer subunit alphaAdd BLAST1224
PeptideiPRO_00000414001 – 35ProxeninAdd BLAST35
PeptideiPRO_00000414011 – 25XeninAdd BLAST25

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei173PhosphoserineCombined sources1
Modified residuei185PhosphothreonineCombined sources1
Modified residuei402PhosphoserineCombined sources1
Modified residuei591PhosphothreonineCombined sources1
Modified residuei895PhosphoserineCombined sources1
Modified residuei965Omega-N-methylarginineCombined sources1
Modified residuei1193PhosphoserineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiP53621.
MaxQBiP53621.
PaxDbiP53621.
PeptideAtlasiP53621.
PRIDEiP53621.

PTM databases

iPTMnetiP53621.
PhosphoSitePlusiP53621.
SwissPalmiP53621.

Expressioni

Tissue specificityi

Uniformly expressed in a wide range of adult and fetal tissues. Xenin is found in gastric, duodenal and jejunal mucosa. Circulates in the blood. Seems to be confined to specific endocrine cells.

Developmental stagei

Xenin is released into the circulation after a meal.

Gene expression databases

BgeeiENSG00000122218.
CleanExiHS_COPA.
GenevisibleiP53621. HS.

Organism-specific databases

HPAiHPA028024.

Interactioni

Subunit structurei

Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Probably interacts with PEX11A. Interacts with SCYL1 (By similarity). Interacts with JAGN1.By similarity1 Publication

Protein-protein interaction databases

BioGridi107709. 86 interactors.
IntActiP53621. 63 interactors.
MINTiMINT-1144184.
STRINGi9606.ENSP00000357048.

Structurei

3D structure databases

ProteinModelPortaliP53621.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati3 – 38WD 1Add BLAST36
Repeati42 – 80WD 2Add BLAST39
Repeati84 – 122WD 3Add BLAST39
Repeati126 – 164WD 4Add BLAST39
Repeati195 – 234WD 5Add BLAST40
Repeati241 – 278WD 6Add BLAST38
Repeati282 – 319WD 7Add BLAST38

Sequence similaritiesi

Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0292. Eukaryota.
ENOG410XPZS. LUCA.
GeneTreeiENSGT00520000055597.
HOGENOMiHOG000195913.
HOVERGENiHBG005379.
InParanoidiP53621.
KOiK05236.
OMAiKSLFMQT.
OrthoDBiEOG091G00SH.
PhylomeDBiP53621.
TreeFamiTF105693.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024977. Apc4_WD40_dom.
IPR016391. Coatomer_asu.
IPR010714. Coatomer_asu_C.
IPR006692. Coatomer_WD-assoc_reg.
IPR020472. G-protein_beta_WD-40_rep.
IPR011048. Haem_d1.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12894. ANAPC4_WD40. 1 hit.
PF04053. Coatomer_WDAD. 1 hit.
PF06957. COPI_C. 1 hit.
PF00400. WD40. 3 hits.
[Graphical view]
PIRSFiPIRSF003354. Coatomer_alpha_subunit. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
SSF51004. SSF51004. 3 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P53621-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLTKFETKSA RVKGLSFHPK RPWILTSLHN GVIQLWDYRM CTLIDKFDEH
60 70 80 90 100
DGPVRGIDFH KQQPLFVSGG DDYKIKVWNY KLRRCLFTLL GHLDYIRTTF
110 120 130 140 150
FHHEYPWILS ASDDQTIRVW NWQSRTCVCV LTGHNHYVMC AQFHPTEDLV
160 170 180 190 200
VSASLDQTVR VWDISGLRKK NLSPGAVESD VRGITGVDLF GTTDAVVKHV
210 220 230 240 250
LEGHDRGVNW AAFHPTMPLI VSGADDRQVK IWRMNESKAW EVDTCRGHYN
260 270 280 290 300
NVSCAVFHPR QELILSNSED KSIRVWDMSK RTGVQTFRRD HDRFWVLAAH
310 320 330 340 350
PNLNLFAAGH DGGMIVFKLE RERPAYAVHG NMLHYVKDRF LRQLDFNSSK
360 370 380 390 400
DVAVMQLRSG SKFPVFNMSY NPAENAVLLC TRASNLENST YDLYTIPKDA
410 420 430 440 450
DSQNPDAPEG KRSSGLTAVW VARNRFAVLD RMHSLLIKNL KNEITKKVQV
460 470 480 490 500
PNCDEIFYAG TGNLLLRDAD SITLFDVQQK RTLASVKISK VKYVIWSADM
510 520 530 540 550
SHVALLAKHA IVICNRKLDA LCNIHENIRV KSGAWDESGV FIYTTSNHIK
560 570 580 590 600
YAVTTGDHGI IRTLDLPIYV TRVKGNNVYC LDRECRPRVL TIDPTEFKFK
610 620 630 640 650
LALINRKYDE VLHMVRNAKL VGQSIIAYLQ KKGYPEVALH FVKDEKTRFS
660 670 680 690 700
LALECGNIEI ALEAAKALDD KNCWEKLGEV ALLQGNHQIV EMCYQRTKNF
710 720 730 740 750
DKLSFLYLIT GNLEKLRKMM KIAEIRKDMS GHYQNALYLG DVSERVRILK
760 770 780 790 800
NCGQKSLAYL TAATHGLDEE AESLKETFDP EKETIPDIDP NAKLLQPPAP
810 820 830 840 850
IMPLDTNWPL LTVSKGFFEG TIASKGKGGA LAADIDIDTV GTEGWGEDAE
860 870 880 890 900
LQLDEDGFVE ATEGLGDDAL GKGQEEGGGW DVEEDLELPP ELDISPGAAG
910 920 930 940 950
GAEDGFFVPP TKGTSPTQIW CNNSQLPVDH ILAGSFETAM RLLHDQVGVI
960 970 980 990 1000
QFGPYKQLFL QTYARGRTTY QALPCLPSMY GYPNRNWKDA GLKNGVPAVG
1010 1020 1030 1040 1050
LKLNDLIQRL QLCYQLTTVG KFEEAVEKFR SILLSVPLLV VDNKQEIAEA
1060 1070 1080 1090 1100
QQLITICREY IVGLSVETER KKLPKETLEQ QKRICEMAAY FTHSNLQPVH
1110 1120 1130 1140 1150
MILVLRTALN LFFKLKNFKT AATFARRLLE LGPKPEVAQQ TRKILSACEK
1160 1170 1180 1190 1200
NPTDAYQLNY DMHNPFDICA ASYRPIYRGK PVEKCPLSGA CYSPEFKGQI
1210 1220
CRVTTVTEIG KDVIGLRISP LQFR
Length:1,224
Mass (Da):138,346
Last modified:September 2, 2008 - v2
Checksum:i5A8BC35CE78F155D
GO
Isoform 2 (identifier: P53621-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     509-509: H → HEHSCPLPLT

Show »
Length:1,233
Mass (Da):139,324
Checksum:iF43D084894C9AD98
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti703L → V in AAB70879 (PubMed:8647451).Curated1

RNA editingi

Edited at position 164.1 Publication
Edited at about 31%.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_066525164I → V in RNA edited version. 1
Natural variantiVAR_073844230K → N in AILJK; causes a defect in retrograde transport from the Golgi to the endoplasmic reticulum. 1 Publication1
Natural variantiVAR_073845233R → H in AILJK. 1 PublicationCorresponds to variant rs794727993dbSNPEnsembl.1
Natural variantiVAR_073846241E → K in AILJK; causes a defect in retrograde transport from the Golgi to the endoplasmic reticulum. 1 PublicationCorresponds to variant rs794727995dbSNPEnsembl.1
Natural variantiVAR_073847243D → G in AILJK. 1 PublicationCorresponds to variant rs794727994dbSNPEnsembl.1
Natural variantiVAR_0338031040V → G.Corresponds to variant rs34997807dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_035043509H → HEHSCPLPLT in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24105 mRNA. Translation: AAB70879.1.
AL513282, AL445230 Genomic DNA. Translation: CAI12454.1.
AL513282, AL445230 Genomic DNA. Translation: CAI12455.1.
AL445230, AL513282 Genomic DNA. Translation: CAI15004.1.
AL445230, AL513282 Genomic DNA. Translation: CAI15005.1.
CH471121 Genomic DNA. Translation: EAW52723.1.
CH471121 Genomic DNA. Translation: EAW52725.1.
BC038447 mRNA. Translation: AAH38447.1.
CCDSiCCDS1202.1. [P53621-1]
CCDS41424.1. [P53621-2]
PIRiJC4668. ERHUAH.
RefSeqiNP_001091868.1. NM_001098398.1. [P53621-2]
NP_004362.2. NM_004371.3. [P53621-1]
UniGeneiHs.162121.
Hs.685025.

Genome annotation databases

EnsembliENST00000241704; ENSP00000241704; ENSG00000122218. [P53621-1]
ENST00000368069; ENSP00000357048; ENSG00000122218. [P53621-2]
GeneIDi1314.
KEGGihsa:1314.
UCSCiuc001fvv.5. human. [P53621-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism, RNA editing

Cross-referencesi

Web resourcesi

Wikipedia

Xenin entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24105 mRNA. Translation: AAB70879.1.
AL513282, AL445230 Genomic DNA. Translation: CAI12454.1.
AL513282, AL445230 Genomic DNA. Translation: CAI12455.1.
AL445230, AL513282 Genomic DNA. Translation: CAI15004.1.
AL445230, AL513282 Genomic DNA. Translation: CAI15005.1.
CH471121 Genomic DNA. Translation: EAW52723.1.
CH471121 Genomic DNA. Translation: EAW52725.1.
BC038447 mRNA. Translation: AAH38447.1.
CCDSiCCDS1202.1. [P53621-1]
CCDS41424.1. [P53621-2]
PIRiJC4668. ERHUAH.
RefSeqiNP_001091868.1. NM_001098398.1. [P53621-2]
NP_004362.2. NM_004371.3. [P53621-1]
UniGeneiHs.162121.
Hs.685025.

3D structure databases

ProteinModelPortaliP53621.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107709. 86 interactors.
IntActiP53621. 63 interactors.
MINTiMINT-1144184.
STRINGi9606.ENSP00000357048.

PTM databases

iPTMnetiP53621.
PhosphoSitePlusiP53621.
SwissPalmiP53621.

Polymorphism and mutation databases

BioMutaiCOPA.
DMDMi205371746.

Proteomic databases

EPDiP53621.
MaxQBiP53621.
PaxDbiP53621.
PeptideAtlasiP53621.
PRIDEiP53621.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000241704; ENSP00000241704; ENSG00000122218. [P53621-1]
ENST00000368069; ENSP00000357048; ENSG00000122218. [P53621-2]
GeneIDi1314.
KEGGihsa:1314.
UCSCiuc001fvv.5. human. [P53621-1]

Organism-specific databases

CTDi1314.
DisGeNETi1314.
GeneCardsiCOPA.
HGNCiHGNC:2230. COPA.
HPAiHPA028024.
MIMi601924. gene.
616414. phenotype.
neXtProtiNX_P53621.
OpenTargetsiENSG00000122218.
PharmGKBiPA26746.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0292. Eukaryota.
ENOG410XPZS. LUCA.
GeneTreeiENSGT00520000055597.
HOGENOMiHOG000195913.
HOVERGENiHBG005379.
InParanoidiP53621.
KOiK05236.
OMAiKSLFMQT.
OrthoDBiEOG091G00SH.
PhylomeDBiP53621.
TreeFamiTF105693.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000122218-MONOMER.
ReactomeiR-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
SignaLinkiP53621.

Miscellaneous databases

ChiTaRSiCOPA. human.
GeneWikiiCOPA_(gene).
GenomeRNAii1314.
PROiP53621.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000122218.
CleanExiHS_COPA.
GenevisibleiP53621. HS.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024977. Apc4_WD40_dom.
IPR016391. Coatomer_asu.
IPR010714. Coatomer_asu_C.
IPR006692. Coatomer_WD-assoc_reg.
IPR020472. G-protein_beta_WD-40_rep.
IPR011048. Haem_d1.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12894. ANAPC4_WD40. 1 hit.
PF04053. Coatomer_WDAD. 1 hit.
PF06957. COPI_C. 1 hit.
PF00400. WD40. 3 hits.
[Graphical view]
PIRSFiPIRSF003354. Coatomer_alpha_subunit. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
SSF51004. SSF51004. 3 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCOPA_HUMAN
AccessioniPrimary (citable) accession number: P53621
Secondary accession number(s): Q5T201, Q8IXZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 2, 2008
Last modified: November 30, 2016
This is version 177 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.