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Protein

Coatomer subunit gamma-1

Gene

COPG1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte triglyceride lipase (PNPLA2) with the lipid droplet surface to mediate lipolysis (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • intracellular protein transport Source: InterPro
  • intra-Golgi vesicle-mediated transport Source: UniProtKB
  • retrograde vesicle-mediated transport, Golgi to ER Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Coatomer subunit gamma-1
Alternative name(s):
Gamma-1-coat protein
Short name:
Gamma-1-COP
Gene namesi
Name:COPG1
Synonyms:COPG
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  • COPI vesicle coat Source: UniProtKB
  • cytosol Source: UniProtKB-SubCell
  • Golgi membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001938571 – 874Coatomer subunit gamma-1Add BLAST874

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei594PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP53620.
PeptideAtlasiP53620.
PRIDEiP53620.

Expressioni

Gene expression databases

BgeeiENSBTAG00000013212.

Interactioni

Subunit structurei

Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Interacts with ZNF289/ARFGAP2 through its C-terminal appendage domain (By similarity). Interacts with EGFR upon EGF treatment; interaction is essential for regulation of EGF-dependent nuclear transport of EGFR by retrograde trafficking from the Golgi to the ER (By similarity). Interacts with COPB1 (By similarity). Interacts with TMED10 (via C-terminus). Interacts with TMED2, TMED3, TMED7 and TMED9.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GBF1Q925382EBI-8511600,EBI-359050From a different organism.

Protein-protein interaction databases

BioGridi160192. 1 interactor.
DIPiDIP-739N.
IntActiP53620. 1 interactor.
STRINGi9913.ENSBTAP00000017588.

Structurei

Secondary structure

1874
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi24 – 30Combined sources7
Helixi31 – 33Combined sources3
Beta strandi36 – 38Combined sources3
Helixi41 – 57Combined sources17
Helixi63 – 74Combined sources12
Helixi75 – 78Combined sources4
Helixi82 – 95Combined sources14
Turni96 – 98Combined sources3
Helixi102 – 105Combined sources4
Helixi106 – 114Combined sources9
Helixi118 – 131Combined sources14
Turni134 – 136Combined sources3
Helixi137 – 148Combined sources12
Helixi153 – 166Combined sources14
Turni167 – 169Combined sources3
Helixi171 – 175Combined sources5
Helixi178 – 184Combined sources7
Helixi190 – 204Combined sources15
Helixi208 – 219Combined sources12
Helixi226 – 236Combined sources11
Helixi264 – 274Combined sources11
Helixi292 – 297Combined sources6
Beta strandi298 – 303Combined sources6
Helixi304 – 309Combined sources6
Helixi609 – 618Combined sources10
Helixi621 – 623Combined sources3
Beta strandi644 – 654Combined sources11
Beta strandi656 – 667Combined sources12
Beta strandi672 – 686Combined sources15
Beta strandi688 – 693Combined sources6
Beta strandi695 – 698Combined sources4
Beta strandi704 – 711Combined sources8
Beta strandi722 – 735Combined sources14
Turni737 – 739Combined sources3
Beta strandi747 – 752Combined sources6
Beta strandi756 – 758Combined sources3
Helixi760 – 763Combined sources4
Beta strandi764 – 766Combined sources3
Helixi772 – 779Combined sources8
Beta strandi785 – 793Combined sources9
Helixi797 – 808Combined sources12
Turni814 – 817Combined sources4
Beta strandi824 – 834Combined sources11
Beta strandi839 – 862Combined sources24
Helixi863 – 872Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PZDX-ray2.31A555-874[»]
3TJZX-ray2.90B/E1-355[»]
ProteinModelPortaliP53620.
SMRiP53620.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53620.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati64 – 101HEAT 1Add BLAST38
Repeati283 – 320HEAT 2Add BLAST38
Repeati322 – 355HEAT 3Add BLAST34
Repeati356 – 392HEAT 4Add BLAST37

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni609 – 874Interaction with ZNF289/ARFGAP2By similarityAdd BLAST266

Sequence similaritiesi

Belongs to the COPG family.Curated
Contains 4 HEAT repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1078. Eukaryota.
COG5240. LUCA.
HOGENOMiHOG000184434.
HOVERGENiHBG004368.
InParanoidiP53620.
KOiK17267.
TreeFamiTF300324.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
2.60.40.1480. 1 hit.
3.30.310.30. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR015873. Clathrin_a/coatomer_app_sub_C.
IPR009028. Coatomer/calthrin_app_sub_C.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR032154. Coatomer_g_Cpla.
IPR017106. Coatomer_gsu.
IPR013040. Coatomer_gsu_app_Ig-like-sub.
[Graphical view]
PANTHERiPTHR10261. PTHR10261. 1 hit.
PfamiPF01602. Adaptin_N. 1 hit.
PF16381. Coatomer_g_Cpla. 1 hit.
PF08752. COP-gamma_platf. 1 hit.
[Graphical view]
PIRSFiPIRSF037093. Coatomer_gamma_subunit. 1 hit.
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49348. SSF49348. 1 hit.
SSF55711. SSF55711. 1 hit.

Sequencei

Sequence statusi: Complete.

P53620-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKKFDKKDE ESGGGSNPFQ HLEKSAVLQE ARVFNETPIN PRKCAHILTK
60 70 80 90 100
ILYLINQGEH LGTTEATEAF FAMTKLFQSN DPTLRRMCYL TIKEMSCIAE
110 120 130 140 150
DVIIVTSSLT KDMTGKEDSY RGPAVRALCQ ITDSTMLQAI ERYMKQAIVD
160 170 180 190 200
KVPSVSSSAL VSSLHLLKCS FDVVKRWVNE AQEAASSDNI MVQYHALGLL
210 220 230 240 250
YHVRKNDRLA VSKMISKFTR HGLKSPFAYC MMIRVASRQL EDEDGSRDSP
260 270 280 290 300
LFDFIESCLR NKHEMVVYEA ASAIVNLPGC SAKELAPAVS VLQLFCSSPK
310 320 330 340 350
AALRYAAVRT LNKVAMKHPS AVTACNLDLE NLVTDANRSI ATLAITTLLK
360 370 380 390 400
TGSEGSIDRL MKQISSFMSE ISDEFKVVVV QAISALCQKY PRKHAVLMNF
410 420 430 440 450
LFSMLREEGG FEYKRAIVDC IISIIEENAE SKETGLSHLC EFIEDCEFTV
460 470 480 490 500
LATRILHLLG QEGPRTSNPS KYIRFIYNRV VLEHAEVRAG AVSALAKFGA
510 520 530 540 550
QNEEMLPSIL VLLKRCVMDD DNEVRDRATF YLNVLEQKQK ALNAGYILNG
560 570 580 590 600
LAVSIPGLER ALQQYTLEPS EKPFDLKSVP LATAPLAEQR TESTPVTAAK
610 620 630 640 650
QPEKVAATRQ EIFQEQLAAV PEFQGLGPLF KSSPEPVALT ESETEYVIRC
660 670 680 690 700
TKHTFTDHMV FQFDCTNTLN DQTLENVTVQ MEPSEAYEVL CYVPARSLPY
710 720 730 740 750
NQPGTCYTLV ALPKEDPTAV ACTFSCVMKF TVKDCDPTTG EADDEGYEDE
760 770 780 790 800
YVLEDLEVTI ADHIQKVMKL NFEAAWDEVG DEFQKEETFT LSTIKTLEEA
810 820 830 840 850
VGNIVKFLGM HPCERSDKVP DNKNTHTLLL AGVFRGGHDI LVRSRLLLLD
860 870
TVTMQVTARS SEELPVDIVL ASVG
Length:874
Mass (Da):97,386
Last modified:October 1, 1996 - v1
Checksum:iA9072FA70C1A0005
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti684S → T in AAI11323 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92987 mRNA. Translation: CAA63574.1.
BC111322 mRNA. Translation: AAI11323.1.
X70019 mRNA. Translation: CAA49616.1.
PIRiS33957.
RefSeqiNP_001032904.1. NM_001037815.2.
UniGeneiBt.65158.

Genome annotation databases

GeneIDi338055.
KEGGibta:338055.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92987 mRNA. Translation: CAA63574.1.
BC111322 mRNA. Translation: AAI11323.1.
X70019 mRNA. Translation: CAA49616.1.
PIRiS33957.
RefSeqiNP_001032904.1. NM_001037815.2.
UniGeneiBt.65158.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PZDX-ray2.31A555-874[»]
3TJZX-ray2.90B/E1-355[»]
ProteinModelPortaliP53620.
SMRiP53620.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi160192. 1 interactor.
DIPiDIP-739N.
IntActiP53620. 1 interactor.
STRINGi9913.ENSBTAP00000017588.

Proteomic databases

PaxDbiP53620.
PeptideAtlasiP53620.
PRIDEiP53620.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi338055.
KEGGibta:338055.

Organism-specific databases

CTDi22820.

Phylogenomic databases

eggNOGiKOG1078. Eukaryota.
COG5240. LUCA.
HOGENOMiHOG000184434.
HOVERGENiHBG004368.
InParanoidiP53620.
KOiK17267.
TreeFamiTF300324.

Miscellaneous databases

EvolutionaryTraceiP53620.

Gene expression databases

BgeeiENSBTAG00000013212.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
2.60.40.1480. 1 hit.
3.30.310.30. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR015873. Clathrin_a/coatomer_app_sub_C.
IPR009028. Coatomer/calthrin_app_sub_C.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR032154. Coatomer_g_Cpla.
IPR017106. Coatomer_gsu.
IPR013040. Coatomer_gsu_app_Ig-like-sub.
[Graphical view]
PANTHERiPTHR10261. PTHR10261. 1 hit.
PfamiPF01602. Adaptin_N. 1 hit.
PF16381. Coatomer_g_Cpla. 1 hit.
PF08752. COP-gamma_platf. 1 hit.
[Graphical view]
PIRSFiPIRSF037093. Coatomer_gamma_subunit. 1 hit.
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49348. SSF49348. 1 hit.
SSF55711. SSF55711. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCOPG1_BOVIN
AccessioniPrimary (citable) accession number: P53620
Secondary accession number(s): Q2T9Q0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.