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Protein

Geranylgeranyl transferase type-1 subunit beta

Gene

Pggt1b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to a cysteine at the fourth position from the C-terminus of proteins with the C-terminal sequence Cys-aliphatic-aliphatic-X. Known substrates include RAC1, RAC2, RAP1A and RAP1B.1 Publication

Catalytic activityi

Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate.1 Publication

Cofactori

Zn2+3 PublicationsNote: Binds 1 zinc ion per subunit.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi269Zinc; catalyticCombined sources1 Publication1
Metal bindingi271Zinc; catalyticCombined sources1 Publication1
Metal bindingi321Zinc; via tele nitrogen; catalyticCombined sources1 Publication1

GO - Molecular functioni

  • CAAX-protein geranylgeranyltransferase activity Source: UniProtKB
  • protein geranylgeranyltransferase activity Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • negative regulation of nitric-oxide synthase biosynthetic process Source: RGD
  • positive regulation of cell cycle Source: RGD
  • positive regulation of cell proliferation Source: RGD
  • protein geranylgeranylation Source: UniProtKB
  • response to cytokine Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Prenyltransferase, Transferase

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Geranylgeranyl transferase type-1 subunit beta (EC:2.5.1.59)
Alternative name(s):
Geranylgeranyl transferase type I subunit beta
Short name:
GGTase-I-beta
Type I protein geranyl-geranyltransferase subunit beta
Gene namesi
Name:Pggt1b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 18

Organism-specific databases

RGDi621754. Pggt1b.

Subcellular locationi

GO - Cellular componenti

  • CAAX-protein geranylgeranyltransferase complex Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2111479.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001197711 – 377Geranylgeranyl transferase type-1 subunit betaAdd BLAST377

Proteomic databases

PaxDbiP53610.
PRIDEiP53610.

Expressioni

Gene expression databases

BgeeiENSRNOG00000003541.
GenevisibleiP53610. RN.

Interactioni

Subunit structurei

Heterodimer of FNTA and PGGT1B. PGGT1B mediates interaction with substrate peptides.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
FntaQ046315EBI-602610,EBI-602447

Protein-protein interaction databases

DIPiDIP-33953N.
IntActiP53610. 1 interactor.
STRINGi10116.ENSRNOP00000004723.

Structurei

Secondary structure

1377
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi22 – 34Combined sources13
Helixi38 – 46Combined sources9
Helixi47 – 60Combined sources14
Helixi64 – 66Combined sources3
Helixi69 – 77Combined sources9
Helixi90 – 92Combined sources3
Beta strandi97 – 99Combined sources3
Helixi122 – 134Combined sources13
Helixi144 – 154Combined sources11
Beta strandi161 – 164Combined sources4
Helixi172 – 184Combined sources13
Helixi188 – 190Combined sources3
Helixi193 – 202Combined sources10
Beta strandi208 – 213Combined sources6
Helixi220 – 233Combined sources14
Helixi236 – 239Combined sources4
Helixi242 – 253Combined sources12
Beta strandi256 – 260Combined sources5
Helixi272 – 282Combined sources11
Helixi286 – 288Combined sources3
Helixi291 – 299Combined sources9
Turni304 – 306Combined sources3
Beta strandi311 – 315Combined sources5
Helixi319 – 331Combined sources13
Turni342 – 345Combined sources4
Helixi348 – 361Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1N4PX-ray2.65B/D/F/H/J/L1-377[»]
1N4QX-ray2.40B/D/F/H/J/L1-377[»]
1N4RX-ray2.80B/D/F/H/J/L1-377[»]
1N4SX-ray2.60B/D/F/H/J/L1-377[»]
1S64X-ray2.55B/D/F/H/J/L1-377[»]
1TNBX-ray2.85B/D/F/H/J/L1-377[»]
1TNOX-ray2.70B/D/F/H/J/L1-377[»]
1TNUX-ray2.70B/D/F/H/J/L1-377[»]
1TNYX-ray2.70B/D/F/H/J/L1-377[»]
1TNZX-ray2.90B/D/F/H/J/L1-377[»]
ProteinModelPortaliP53610.
SMRiP53610.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53610.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati144 – 186PFTB 1Sequence analysisAdd BLAST43
Repeati193 – 234PFTB 2Sequence analysisAdd BLAST42
Repeati245 – 284PFTB 3Sequence analysisAdd BLAST40
Repeati291 – 333PFTB 4Sequence analysisAdd BLAST43

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni219 – 221Geranylgeranyl diphosphate bindingCombined sources1 Publication3
Regioni263 – 266Geranylgeranyl diphosphate bindingCombined sources1 Publication4
Regioni272 – 275Geranylgeranyl diphosphate bindingCombined sources1 Publication4

Sequence similaritiesi

Contains 4 PFTB repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0367. Eukaryota.
COG5029. LUCA.
GeneTreeiENSGT00530000063392.
HOGENOMiHOG000180333.
HOVERGENiHBG008181.
InParanoidiP53610.
KOiK11713.
OMAiQMDGFQG.
OrthoDBiEOG091G01YZ.
PhylomeDBiP53610.
TreeFamiTF105968.

Family and domain databases

Gene3Di1.50.10.20. 1 hit.
InterProiIPR001330. PFTB_repeat.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00432. Prenyltrans. 4 hits.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.

Sequencei

Sequence statusi: Complete.

P53610-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATEDDRLA GSGEGERLDF LRDRHVRFFQ RCLQVLPERY SSLETSRLTI
60 70 80 90 100
AFFALSGLDM LDSLDVVNKD DIIEWIYSLQ VLPTEDRSNL DRCGFRGSSY
110 120 130 140 150
LGIPFNPSKN PGTAHPYDSG HIAMTYTGLS CLIILGDDLS RVDKEACLAG
160 170 180 190 200
LRALQLEDGS FCAVPEGSEN DMRFVYCASC ICYMLNNWSG MDMKKAISYI
210 220 230 240 250
RRSMSYDNGL AQGAGLESHG GSTFCGIASL CLMGKLEEVF SEKELNRIKR
260 270 280 290 300
WCIMRQQNGY HGRPNKPVDT CYSFWVGATL KLLKIFQYTN FEKNRNYILS
310 320 330 340 350
TQDRLVGGFA KWPDSHPDAL HAYFGICGLS LMEESGICKV HPALNVSTRT
360 370
SERLRDLHQS WKTKDSKQCS DNVHISS
Length:377
Mass (Da):42,414
Last modified:October 1, 1996 - v1
Checksum:iAADEC7301A4A4011
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24116 mRNA. Translation: AAA17756.1.
PIRiB53044.
RefSeqiNP_112344.1. NM_031082.1.
UniGeneiRn.10031.

Genome annotation databases

EnsembliENSRNOT00000004723; ENSRNOP00000004723; ENSRNOG00000003541.
GeneIDi81746.
KEGGirno:81746.
UCSCiRGD:621754. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24116 mRNA. Translation: AAA17756.1.
PIRiB53044.
RefSeqiNP_112344.1. NM_031082.1.
UniGeneiRn.10031.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1N4PX-ray2.65B/D/F/H/J/L1-377[»]
1N4QX-ray2.40B/D/F/H/J/L1-377[»]
1N4RX-ray2.80B/D/F/H/J/L1-377[»]
1N4SX-ray2.60B/D/F/H/J/L1-377[»]
1S64X-ray2.55B/D/F/H/J/L1-377[»]
1TNBX-ray2.85B/D/F/H/J/L1-377[»]
1TNOX-ray2.70B/D/F/H/J/L1-377[»]
1TNUX-ray2.70B/D/F/H/J/L1-377[»]
1TNYX-ray2.70B/D/F/H/J/L1-377[»]
1TNZX-ray2.90B/D/F/H/J/L1-377[»]
ProteinModelPortaliP53610.
SMRiP53610.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-33953N.
IntActiP53610. 1 interactor.
STRINGi10116.ENSRNOP00000004723.

Chemistry databases

ChEMBLiCHEMBL2111479.

Proteomic databases

PaxDbiP53610.
PRIDEiP53610.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000004723; ENSRNOP00000004723; ENSRNOG00000003541.
GeneIDi81746.
KEGGirno:81746.
UCSCiRGD:621754. rat.

Organism-specific databases

CTDi5229.
RGDi621754. Pggt1b.

Phylogenomic databases

eggNOGiKOG0367. Eukaryota.
COG5029. LUCA.
GeneTreeiENSGT00530000063392.
HOGENOMiHOG000180333.
HOVERGENiHBG008181.
InParanoidiP53610.
KOiK11713.
OMAiQMDGFQG.
OrthoDBiEOG091G01YZ.
PhylomeDBiP53610.
TreeFamiTF105968.

Miscellaneous databases

EvolutionaryTraceiP53610.
PROiP53610.

Gene expression databases

BgeeiENSRNOG00000003541.
GenevisibleiP53610. RN.

Family and domain databases

Gene3Di1.50.10.20. 1 hit.
InterProiIPR001330. PFTB_repeat.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00432. Prenyltrans. 4 hits.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPGTB1_RAT
AccessioniPrimary (citable) accession number: P53610
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.