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Protein

MAP kinase kinase kinase SSK2

Gene

SSK2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Activates the PBS2 MAP kinase kinase by phosphorylation.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei1295 – 12951ATPPROSITE-ProRule annotation
Active sitei1390 – 13901Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1272 – 12809ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • actin binding Source: SGD
  • ATP binding Source: UniProtKB-KW
  • MAP kinase kinase kinase activity Source: SGD

GO - Biological processi

  • cellular hyperosmotic response Source: SGD
  • MAPK cascade involved in osmosensory signaling pathway Source: SGD
  • osmosensory signaling via phosphorelay pathway Source: SGD
  • positive regulation of actin cytoskeleton reorganization Source: SGD
  • protein autophosphorylation Source: SGD
  • protein phosphorylation Source: SGD
  • stress-activated MAPK cascade Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33342-MONOMER.
BRENDAi2.7.11.25. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
MAP kinase kinase kinase SSK2 (EC:2.7.11.25)
Alternative name(s):
Suppressor of sensor kinase 2
Gene namesi
Name:SSK2
Ordered Locus Names:YNR031C
ORF Names:N3276
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNR031C.
SGDiS000005314. SSK2.

Subcellular locationi

GO - Cellular componenti

  • cell cortex Source: SGD
  • cellular bud neck Source: SGD
  • cellular bud tip Source: SGD
  • cytosol Source: SGD
  • incipient cellular bud site Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15791579MAP kinase kinase kinase SSK2PRO_0000086682Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei57 – 571PhosphoserineCombined sources
Modified residuei62 – 621PhosphoserineCombined sources
Modified residuei78 – 781PhosphoserineCombined sources
Modified residuei118 – 1181PhosphoserineCombined sources
Modified residuei290 – 2901PhosphoserineCombined sources
Modified residuei1424 – 14241PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53599.
TopDownProteomicsiP53599.

PTM databases

iPTMnetiP53599.

Interactioni

Subunit structurei

Interacts with by SSK1.

Binary interactionsi

WithEntry#Exp.IntActNotes
SLT2Q007722EBI-18191,EBI-17372
SSK1Q070846EBI-18191,EBI-18184

GO - Molecular functioni

  • actin binding Source: SGD

Protein-protein interaction databases

BioGridi35855. 158 interactions.
DIPiDIP-2436N.
IntActiP53599. 9 interactions.
MINTiMINT-691754.

Structurei

3D structure databases

ProteinModelPortaliP53599.
SMRiP53599. Positions 1184-1558.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1266 – 1558293Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00590000083157.
HOGENOMiHOG000207441.
InParanoidiP53599.
KOiK11230.
OMAiAPWILKA.
OrthoDBiEOG092C05H5.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR017240. MAPKKK_Ssk2/Ssk22.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037579. MAPKKK_SSK22. 1 hit.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53599-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHSDYFNYK PYGDSTEKPS SSKMRQSSSS SSSRLRSESL GRNSNTTQAR
60 70 80 90 100
VASSPISPGL HSTQYFRSPN AVYSPGESPL NTVQLFNRLP GIPQGQFFHQ
110 120 130 140 150
NAISGSSSSS ARSSRRPSNI GLPLPKNPQQ SLPKLSTQPV PVHKKVEASK
160 170 180 190 200
TESEIIKKPA PVNSNQDPLL TTPTLVISPE LASLNTTNTS IMSTPQNITN
210 220 230 240 250
QTSNKHIPTR SQPNGSTSSS TLQDIVTTNS SQRSVGHHGG STTSLRTYKK
260 270 280 290 300
QYVLNEQLYL RKMRNRANDD YYTRGIVASS NFEDDEENFS NKGEDDLELE
310 320 330 340 350
MDDLLKVEGE DKDNDFNFGY NFITSSTKNN ENVVSMSLNY LKGKLDWLRD
360 370 380 390 400
VNNDQPCEIE DEEWHSILGS EDLLSKLLQN PMVNNRFEWQ TMLSKVLKGD
410 420 430 440 450
IVRNEKTKIA NQGKGPGFNT QFSDDIWIEL KAWMNGRTVE DQNKSLRIFR
460 470 480 490 500
DSTDSVFQEI MAFKLEDNMS ADEAAETIKS LVDKYYRVLN LWPNIKRMHA
510 520 530 540 550
EKPITKTEAF RNRIDTLNSW LNFKFNFDTN IAYLKKWIVG NKELESTTEV
560 570 580 590 600
DNTTVNLDDP AVFATNCKRF AEQIMKEKDI ELIFQKKIFF PLAPWILKAK
610 620 630 640 650
FFFLKYQKTW NELNLSYLDQ DLEFLLMFPM RLVKDIILIR LSYAKKIQNP
660 670 680 690 700
TLMMIDQMMD DFSTYIKLAV QMKFTVASYC NDWFFKVKID PEFDHTVVEG
710 720 730 740 750
LEYFFSILEL RILYSGKNSF KTSKEPDLLL KYWEMFRNVG YYIDDAGELI
760 770 780 790 800
AAEFTKLTLR LVHRLHAYLL RQQNTPPKLE NEAAAEKWLV QIFEILGSMK
810 820 830 840 850
RKLNRFTNIL TKAFQNFVRY KIEDHNYLLK QLKETGHFLI YTGGYLEQNG
860 870 880 890 900
TYLIGSPELL GCKDDDILRI IKNSDIGCDL VPKLEINNSL TIYNALDDNW
910 920 930 940 950
NSNSSLGSDI SNDGTPFYYI KNDLTTQPRS YNGNRVNREP DFENSRSTEE
960 970 980 990 1000
EFYELETRLN SLGYVLVLTP QEPLLWEGEM YNLSDNKTIK PEGLNLKVIP
1010 1020 1030 1040 1050
NSIDLMCQGS SYALEYQCDR FQQISGSSVS FLEKKSSSET VKNNLQRINK
1060 1070 1080 1090 1100
AYFRCTYSVL KNYTKIVTTF KKVSPVNDLL NNIFLFGRDF GLNFLRINVA
1110 1120 1130 1140 1150
NNEKRSIIIL LMMRLSIGWL KFLAEDCDPT DQRVFRWCVT SMEFAMHMVS
1160 1170 1180 1190 1200
GWNILALDEC QFSSLKQKIS ECMSLLISHF DIIGARSIEV EKINQQARSN
1210 1220 1230 1240 1250
LDLEDVFDDD MMLQVNSEFR VQSIMELEER IKRNPHQTGK VIDDSDKGNK
1260 1270 1280 1290 1300
YLVSLASSIS NVSMRWQKRN FIGGGTFGRV YSAVDLDNGE ILAVKEINIQ
1310 1320 1330 1340 1350
DSKSMQKIFP LIKEEMSVLE ILNHPNIVSY YGVEVHRDKV NIFMEYCEGG
1360 1370 1380 1390 1400
SLAALLEHGR IEDEMVTQVY TLQLLEGLAY LHESGIVHRD VKPENILLDF
1410 1420 1430 1440 1450
NGVIKYVDFG AAKKIANNGT RLASMNKIEN ADGEHEDVTH VSDSKAVKNN
1460 1470 1480 1490 1500
ENALLDMMGT PMYMAPESIT GSTTKGKLGA DDVWSLGCVV LEMITGRRPW
1510 1520 1530 1540 1550
ANLDNEWAIM YHVAAGHTPQ FPTKDEVSSA GMKFLERCLI QNPSKRASAV
1560 1570
ELLMDPWIVQ IREIAFGDDS SSTDTEERE
Length:1,579
Mass (Da):180,528
Last modified:October 1, 1996 - v1
Checksum:i91A07A195CB3772E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41927 Genomic DNA. Translation: AAC41665.1.
Z71646 Genomic DNA. Translation: CAA96311.1.
BK006947 Genomic DNA. Translation: DAA10571.1.
PIRiS59801.
RefSeqiNP_014428.1. NM_001183208.1.

Genome annotation databases

EnsemblFungiiYNR031C; YNR031C; YNR031C.
GeneIDi855765.
KEGGisce:YNR031C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41927 Genomic DNA. Translation: AAC41665.1.
Z71646 Genomic DNA. Translation: CAA96311.1.
BK006947 Genomic DNA. Translation: DAA10571.1.
PIRiS59801.
RefSeqiNP_014428.1. NM_001183208.1.

3D structure databases

ProteinModelPortaliP53599.
SMRiP53599. Positions 1184-1558.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35855. 158 interactions.
DIPiDIP-2436N.
IntActiP53599. 9 interactions.
MINTiMINT-691754.

PTM databases

iPTMnetiP53599.

Proteomic databases

MaxQBiP53599.
TopDownProteomicsiP53599.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNR031C; YNR031C; YNR031C.
GeneIDi855765.
KEGGisce:YNR031C.

Organism-specific databases

EuPathDBiFungiDB:YNR031C.
SGDiS000005314. SSK2.

Phylogenomic databases

GeneTreeiENSGT00590000083157.
HOGENOMiHOG000207441.
InParanoidiP53599.
KOiK11230.
OMAiAPWILKA.
OrthoDBiEOG092C05H5.

Enzyme and pathway databases

BioCyciYEAST:G3O-33342-MONOMER.
BRENDAi2.7.11.25. 984.

Miscellaneous databases

PROiP53599.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR017240. MAPKKK_Ssk2/Ssk22.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037579. MAPKKK_SSK22. 1 hit.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSSK2_YEAST
AccessioniPrimary (citable) accession number: P53599
Secondary accession number(s): D6W1K5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 217 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.