P53599 (SSK2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
MAP kinase kinase kinase SSK2
EC=2.7.11.25

Alternative name(s):
Suppressor of sensor kinase 2

Gene names

Name:	SSK2
Ordered Locus Names:	YNR031C
ORF Names:	N3276

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1579 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Activates the PBS2 MAP kinase kinase by phosphorylation.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with by SSK1.
Miscellaneous	Present with 217 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	MAPKKK cascade involved in osmosensory signaling pathway Inferred from mutant phenotype Ref.1. Source: SGD actin cytoskeleton organization Inferred from direct assay. Source: SGD osmosensory signaling pathway via two-component system Inferred from physical interaction. Source: SGD protein autophosphorylation Inferred from direct assay. Source: SGD
Cellular component	cellular bud neck Inferred from direct assay. Source: SGD cellular bud tip Inferred from direct assay. Source: SGD cytosol Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activity Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SLT2	Q00772	2	EBI-18191,EBI-17372
SSK1	Q07084	7	EBI-18191,EBI-18184

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1579	1579	MAP kinase kinase kinase SSK2		PRO_0000086682
Regions
	Domain	1266 – 1558	293	Protein kinase
	Nucleotide binding	1272 – 1280	9	ATP By similarity
Sites
	Active site	1390	1	Proton acceptor By similarity
	Binding site	1295	1	ATP By similarity
Amino acid modifications
	Modified residue	54	1	Phosphoserine Ref.6
	Modified residue	57	1	Phosphoserine Ref.6
	Modified residue	74	1	Phosphoserine Ref.6
	Modified residue	78	1	Phosphoserine Ref.6
	Modified residue	82	1	Phosphothreonine Ref.6
	Modified residue	118	1	Phosphoserine Ref.5 Ref.6
	Modified residue	290	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P53599 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 91A07A195CB3772E	FASTA	1,579	180,528
	10 20 30 40 50 60 MSHSDYFNYK PYGDSTEKPS SSKMRQSSSS SSSRLRSESL GRNSNTTQAR VASSPISPGL 70 80 90 100 110 120 HSTQYFRSPN AVYSPGESPL NTVQLFNRLP GIPQGQFFHQ NAISGSSSSS ARSSRRPSNI 130 140 150 160 170 180 GLPLPKNPQQ SLPKLSTQPV PVHKKVEASK TESEIIKKPA PVNSNQDPLL TTPTLVISPE 190 200 210 220 230 240 LASLNTTNTS IMSTPQNITN QTSNKHIPTR SQPNGSTSSS TLQDIVTTNS SQRSVGHHGG 250 260 270 280 290 300 STTSLRTYKK QYVLNEQLYL RKMRNRANDD YYTRGIVASS NFEDDEENFS NKGEDDLELE 310 320 330 340 350 360 MDDLLKVEGE DKDNDFNFGY NFITSSTKNN ENVVSMSLNY LKGKLDWLRD VNNDQPCEIE 370 380 390 400 410 420 DEEWHSILGS EDLLSKLLQN PMVNNRFEWQ TMLSKVLKGD IVRNEKTKIA NQGKGPGFNT 430 440 450 460 470 480 QFSDDIWIEL KAWMNGRTVE DQNKSLRIFR DSTDSVFQEI MAFKLEDNMS ADEAAETIKS 490 500 510 520 530 540 LVDKYYRVLN LWPNIKRMHA EKPITKTEAF RNRIDTLNSW LNFKFNFDTN IAYLKKWIVG 550 560 570 580 590 600 NKELESTTEV DNTTVNLDDP AVFATNCKRF AEQIMKEKDI ELIFQKKIFF PLAPWILKAK 610 620 630 640 650 660 FFFLKYQKTW NELNLSYLDQ DLEFLLMFPM RLVKDIILIR LSYAKKIQNP TLMMIDQMMD 670 680 690 700 710 720 DFSTYIKLAV QMKFTVASYC NDWFFKVKID PEFDHTVVEG LEYFFSILEL RILYSGKNSF 730 740 750 760 770 780 KTSKEPDLLL KYWEMFRNVG YYIDDAGELI AAEFTKLTLR LVHRLHAYLL RQQNTPPKLE 790 800 810 820 830 840 NEAAAEKWLV QIFEILGSMK RKLNRFTNIL TKAFQNFVRY KIEDHNYLLK QLKETGHFLI 850 860 870 880 890 900 YTGGYLEQNG TYLIGSPELL GCKDDDILRI IKNSDIGCDL VPKLEINNSL TIYNALDDNW 910 920 930 940 950 960 NSNSSLGSDI SNDGTPFYYI KNDLTTQPRS YNGNRVNREP DFENSRSTEE EFYELETRLN 970 980 990 1000 1010 1020 SLGYVLVLTP QEPLLWEGEM YNLSDNKTIK PEGLNLKVIP NSIDLMCQGS SYALEYQCDR 1030 1040 1050 1060 1070 1080 FQQISGSSVS FLEKKSSSET VKNNLQRINK AYFRCTYSVL KNYTKIVTTF KKVSPVNDLL 1090 1100 1110 1120 1130 1140 NNIFLFGRDF GLNFLRINVA NNEKRSIIIL LMMRLSIGWL KFLAEDCDPT DQRVFRWCVT 1150 1160 1170 1180 1190 1200 SMEFAMHMVS GWNILALDEC QFSSLKQKIS ECMSLLISHF DIIGARSIEV EKINQQARSN 1210 1220 1230 1240 1250 1260 LDLEDVFDDD MMLQVNSEFR VQSIMELEER IKRNPHQTGK VIDDSDKGNK YLVSLASSIS 1270 1280 1290 1300 1310 1320 NVSMRWQKRN FIGGGTFGRV YSAVDLDNGE ILAVKEINIQ DSKSMQKIFP LIKEEMSVLE 1330 1340 1350 1360 1370 1380 ILNHPNIVSY YGVEVHRDKV NIFMEYCEGG SLAALLEHGR IEDEMVTQVY TLQLLEGLAY 1390 1400 1410 1420 1430 1440 LHESGIVHRD VKPENILLDF NGVIKYVDFG AAKKIANNGT RLASMNKIEN ADGEHEDVTH 1450 1460 1470 1480 1490 1500 VSDSKAVKNN ENALLDMMGT PMYMAPESIT GSTTKGKLGA DDVWSLGCVV LEMITGRRPW 1510 1520 1530 1540 1550 1560 ANLDNEWAIM YHVAAGHTPQ FPTKDEVSSA GMKFLERCLI QNPSKRASAV ELLMDPWIVQ 1570 IREIAFGDDS SSTDTEERE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Activation of yeast PBS2 MAPKK by MAPKKKs or by binding of an SH3-containing osmosensor." Maeda T., Takekawa M., Saito H. Science 269:554-558(1995) [PubMed: 7624781] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-118, MASS SPECTROMETRY. Strain: ADR376.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-54; SER-57; SER-74; SER-78; THR-82; SER-118 AND SER-290, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L41927 Genomic DNA. Translation: AAC41665.1. Z71646 Genomic DNA. Translation: CAA96311.1. BK006947 Genomic DNA. Translation: DAA10571.1.
PIR	S59801.
RefSeq	NP_014428.1. NM_001183208.1.
3D structure databases
ProteinModelPortal	P53599.
SMR	P53599. Positions 1269-1558.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2436N.
IntAct	P53599. 14 interactions.
MINT	MINT-691754.
STRING	P53599.
Proteomic databases
PeptideAtlas	P53599.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNR031C; YNR031C; YNR031C.
GeneID	855765.
KEGG	sce:YNR031C.
NMPDR	fig|4932.3.peg.5508.
Organism-specific databases
CYGD	YNR031c.
SGD	S000005314. SSK2.
Phylogenomic databases
eggNOG	fuNOG05884.
GeneTree	EFGT00050000000597.
HOGENOM	HBG398769.
OMA	ISHFDIM.
OrthoDB	EOG4GF6P7.
Enzyme and pathway databases
BRENDA	2.7.11.25. 984.
Gene expression databases
ArrayExpress	P53599.
Genevestigator	P53599.
GermOnline	YNR031C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR017240. MAPKKK_SSK22. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K11230.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PIRSF	PIRSF037579. MAPKKK_SSK22. 1 hit.
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980205.

Entry information

Entry name

SSK2_YEAST

Accession

Primary (citable) accession number: P53599
Secondary accession number(s): D6W1K5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program