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Protein

Succinyl-CoA ligase [ADP-forming] subunit alpha, mitochondrial

Gene

LSC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + succinate + CoA = ADP + phosphate + succinyl-CoA.1 Publication

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes succinate from succinyl-CoA (ligase route).
Proteins known to be involved in this subpathway in this organism are:
  1. Succinyl-CoA ligase [ADP-forming] subunit alpha, mitochondrial (LSC1), Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial (LSC2)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinate from succinyl-CoA (ligase route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei284 – 2841Tele-phosphohistidine intermediateBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cofactor binding Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • succinate-CoA ligase (ADP-forming) activity Source: SGD

GO - Biological processi

  • succinyl-CoA metabolic process Source: SGD
  • tricarboxylic acid cycle Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YOR142W-MONOMER.
ReactomeiR-SCE-163765. ChREBP activates metabolic gene expression.
R-SCE-71403. Citric acid cycle (TCA cycle).
R-SCE-75105. Fatty Acyl-CoA Biosynthesis.
UniPathwayiUPA00223; UER00999.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinyl-CoA ligase [ADP-forming] subunit alpha, mitochondrial (EC:6.2.1.5)
Alternative name(s):
Succinyl-CoA synthetase subunit alpha
Short name:
SCS-alpha
Gene namesi
Name:LSC1
Ordered Locus Names:YOR142W
ORF Names:YOR3352W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR142W.
SGDiS000005668. LSC1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial nucleoid Source: SGD
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 329Succinyl-CoA ligase [ADP-forming] subunit alpha, mitochondrialPRO_0000033351
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

MaxQBiP53598.
PeptideAtlasiP53598.

2D gel databases

UCD-2DPAGEP53598.

PTM databases

iPTMnetiP53598.

Expressioni

Inductioni

Induced during growth on nonfermentable carbon sources and repressed during growth on glucose.1 Publication

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit.By similarity

Protein-protein interaction databases

BioGridi34536. 57 interactions.
DIPiDIP-6542N.
IntActiP53598. 6 interactions.
MINTiMINT-693383.

Structurei

3D structure databases

ProteinModelPortaliP53598.
SMRiP53598. Positions 24-329.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00530000063275.
HOGENOMiHOG000239685.
InParanoidiP53598.
KOiK01899.
OMAiFEQDPQT.
OrthoDBiEOG7XPZGS.

Family and domain databases

Gene3Di3.40.50.261. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR017440. Cit_synth/succinyl-CoA_lig_AS.
IPR003781. CoA-bd.
IPR005810. CoA_lig_alpha.
IPR005811. CoA_ligase.
IPR016040. NAD(P)-bd_dom.
IPR016102. Succinyl-CoA_synth-like.
[Graphical view]
PfamiPF02629. CoA_binding. 1 hit.
PF00549. Ligase_CoA. 1 hit.
[Graphical view]
PIRSFiPIRSF001553. SucCS_alpha. 1 hit.
PRINTSiPR01798. SCOASYNTHASE.
SMARTiSM00881. CoA_binding. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF52210. SSF52210. 1 hit.
PROSITEiPS01216. SUCCINYL_COA_LIG_1. 1 hit.
PS00399. SUCCINYL_COA_LIG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53598-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRSTVSKAS LKICRHFHRE SIPYDKTIKN LLLPKDTKVI FQGFTGKQGT
60 70 80 90 100
FHASISQEYG TNVVGGTNPK KAGQTHLGQP VFASVKDAIK ETGATASAIF
110 120 130 140 150
VPPPIAAAAI KESIEAEIPL AVCITEGIPQ HDMLYIAEML QTQDKTRLVG
160 170 180 190 200
PNCPGIINPA TKVRIGIQPP KIFQAGKIGI ISRSGTLTYE AVQQTTKTDL
210 220 230 240 250
GQSLVIGMGG DAFPGTDFID ALKLFLEDET TEGIIMLGEI GGKAEIEAAQ
260 270 280 290 300
FLKEYNFSRS KPMPVASFIA GTVAGQMKGV RMGHSGAIVE GSGTDAESKK
310 320
QALRDVGVAV VESPGYLGQA LLDQFAKFK
Length:329
Mass (Da):35,032
Last modified:October 1, 1996 - v1
Checksum:i17DD3AFC47E92644
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94335 Genomic DNA. Translation: CAA64059.1.
Z75050 Genomic DNA. Translation: CAA99342.1.
BK006948 Genomic DNA. Translation: DAA10914.1.
PIRiS61696.
RefSeqiNP_014785.3. NM_001183561.3.

Genome annotation databases

EnsemblFungiiYOR142W; YOR142W; YOR142W.
GeneIDi854310.
KEGGisce:YOR142W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94335 Genomic DNA. Translation: CAA64059.1.
Z75050 Genomic DNA. Translation: CAA99342.1.
BK006948 Genomic DNA. Translation: DAA10914.1.
PIRiS61696.
RefSeqiNP_014785.3. NM_001183561.3.

3D structure databases

ProteinModelPortaliP53598.
SMRiP53598. Positions 24-329.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34536. 57 interactions.
DIPiDIP-6542N.
IntActiP53598. 6 interactions.
MINTiMINT-693383.

PTM databases

iPTMnetiP53598.

2D gel databases

UCD-2DPAGEP53598.

Proteomic databases

MaxQBiP53598.
PeptideAtlasiP53598.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR142W; YOR142W; YOR142W.
GeneIDi854310.
KEGGisce:YOR142W.

Organism-specific databases

EuPathDBiFungiDB:YOR142W.
SGDiS000005668. LSC1.

Phylogenomic databases

GeneTreeiENSGT00530000063275.
HOGENOMiHOG000239685.
InParanoidiP53598.
KOiK01899.
OMAiFEQDPQT.
OrthoDBiEOG7XPZGS.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00999.
BioCyciYEAST:YOR142W-MONOMER.
ReactomeiR-SCE-163765. ChREBP activates metabolic gene expression.
R-SCE-71403. Citric acid cycle (TCA cycle).
R-SCE-75105. Fatty Acyl-CoA Biosynthesis.

Miscellaneous databases

PROiP53598.

Family and domain databases

Gene3Di3.40.50.261. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR017440. Cit_synth/succinyl-CoA_lig_AS.
IPR003781. CoA-bd.
IPR005810. CoA_lig_alpha.
IPR005811. CoA_ligase.
IPR016040. NAD(P)-bd_dom.
IPR016102. Succinyl-CoA_synth-like.
[Graphical view]
PfamiPF02629. CoA_binding. 1 hit.
PF00549. Ligase_CoA. 1 hit.
[Graphical view]
PIRSFiPIRSF001553. SucCS_alpha. 1 hit.
PRINTSiPR01798. SCOASYNTHASE.
SMARTiSM00881. CoA_binding. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF52210. SSF52210. 1 hit.
PROSITEiPS01216. SUCCINYL_COA_LIG_1. 1 hit.
PS00399. SUCCINYL_COA_LIG_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: FUNCTION, CATALYTIC ACTIVITY, INDUCTION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  8. "Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase."
    Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.
    Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSUCA_YEAST
AccessioniPrimary (citable) accession number: P53598
Secondary accession number(s): D6W2J8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 18400 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.