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Protein

Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial

Gene

LSC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA (PubMed:9874242). The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit (By similarity).UniRule annotation1 Publication

Miscellaneous

Present with 18400 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + succinate + CoA = ADP + phosphate + succinyl-CoA.UniRule annotation1 Publication

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes succinate from succinyl-CoA (ligase route).UniRule annotation1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (LSC2), Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial (LSC1)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinate from succinyl-CoA (ligase route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei71Coenzyme AUniRule annotation1
Binding sitei189Substrate; shared with subunit betaUniRule annotation1
Active sitei284Tele-phosphohistidine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • succinyl-CoA metabolic process Source: SGD
  • tricarboxylic acid cycle Source: SGD

Keywordsi

Molecular functionLigase
Biological processTricarboxylic acid cycle
LigandNucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YOR142W-MONOMER.
ReactomeiR-SCE-163765. ChREBP activates metabolic gene expression.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-71403. Citric acid cycle (TCA cycle).
R-SCE-75105. Fatty acyl-CoA biosynthesis.
UniPathwayiUPA00223; UER00999.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrialUniRule annotation1 Publication (EC:6.2.1.5UniRule annotation1 Publication)
Alternative name(s):
Succinyl-CoA synthetase subunit alphaUniRule annotation
Short name:
SCS-alphaUniRule annotation
Gene namesi
Name:LSC11 Publication
Ordered Locus Names:YOR142WImported
ORF Names:YOR3352W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR142W.
SGDiS000005668. LSC1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 24MitochondrionUniRule annotationAdd BLAST24
ChainiPRO_000003335125 – 329Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrialUniRule annotationAdd BLAST305

Proteomic databases

MaxQBiP53598.
PRIDEiP53598.

2D gel databases

UCD-2DPAGEiP53598.

PTM databases

iPTMnetiP53598.

Expressioni

Inductioni

Induced during growth on nonfermentable carbon sources and repressed during growth on glucose.1 Publication

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit.UniRule annotation

Protein-protein interaction databases

BioGridi34536. 150 interactors.
DIPiDIP-6542N.
IntActiP53598. 8 interactors.
MINTiMINT-693383.
STRINGi4932.YOR142W.

Structurei

3D structure databases

ProteinModelPortaliP53598.
SMRiP53598.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni45 – 48Coenzyme A bindingUniRule annotation4
Regioni124 – 126Coenzyme A bindingUniRule annotation3

Sequence similaritiesi

Belongs to the succinate/malate CoA ligase alpha subunit family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00530000063275.
HOGENOMiHOG000239685.
InParanoidiP53598.
KOiK01899.
OMAiIIFVPPA.
OrthoDBiEOG092C3IM6.

Family and domain databases

Gene3Di3.40.50.261. 1 hit.
HAMAPiMF_01988. Succ_CoA_alpha. 1 hit.
InterProiView protein in InterPro
IPR017440. Cit_synth/succinyl-CoA_lig_AS.
IPR033847. Citrt_syn/SCS-alpha_CS.
IPR003781. CoA-bd.
IPR005810. CoA_lig_alpha.
IPR005811. CoA_ligase.
IPR036291. NAD(P)-bd_dom_sf.
IPR016102. Succinyl-CoA_synth-like.
PfamiView protein in Pfam
PF02629. CoA_binding. 1 hit.
PF00549. Ligase_CoA. 1 hit.
PIRSFiPIRSF001553. SucCS_alpha. 1 hit.
SMARTiView protein in SMART
SM00881. CoA_binding. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF52210. SSF52210. 1 hit.
PROSITEiView protein in PROSITE
PS01216. SUCCINYL_COA_LIG_1. 1 hit.
PS00399. SUCCINYL_COA_LIG_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53598-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRSTVSKAS LKICRHFHRE SIPYDKTIKN LLLPKDTKVI FQGFTGKQGT
60 70 80 90 100
FHASISQEYG TNVVGGTNPK KAGQTHLGQP VFASVKDAIK ETGATASAIF
110 120 130 140 150
VPPPIAAAAI KESIEAEIPL AVCITEGIPQ HDMLYIAEML QTQDKTRLVG
160 170 180 190 200
PNCPGIINPA TKVRIGIQPP KIFQAGKIGI ISRSGTLTYE AVQQTTKTDL
210 220 230 240 250
GQSLVIGMGG DAFPGTDFID ALKLFLEDET TEGIIMLGEI GGKAEIEAAQ
260 270 280 290 300
FLKEYNFSRS KPMPVASFIA GTVAGQMKGV RMGHSGAIVE GSGTDAESKK
310 320
QALRDVGVAV VESPGYLGQA LLDQFAKFK
Length:329
Mass (Da):35,032
Last modified:October 1, 1996 - v1
Checksum:i17DD3AFC47E92644
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94335 Genomic DNA. Translation: CAA64059.1.
Z75050 Genomic DNA. Translation: CAA99342.1.
BK006948 Genomic DNA. Translation: DAA10914.1.
PIRiS61696.
RefSeqiNP_014785.3. NM_001183561.3.

Genome annotation databases

EnsemblFungiiYOR142W; YOR142W; YOR142W.
GeneIDi854310.
KEGGisce:YOR142W.

Similar proteinsi

Entry informationi

Entry nameiSUCA_YEAST
AccessioniPrimary (citable) accession number: P53598
Secondary accession number(s): D6W2J8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 25, 2017
This is version 171 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names